Acupuncture Points ; Acupuncture Therapy ; Adult ; Esophageal Achalasia ; therapy ; Humans ; Male

AIM: To clarify the role and mechanism of hippocampus in the formation of P3-like. METHODS: (1) The hippocampal and parietal P3-like were simultaneously recorded by chronically implanted electrodes in rats. (2) In forebrain ischemia/reperfusion model, the effects of the selective damage of hippocampal CA1 subfield on the hippocampal and parietal P3-like were examined. (3) The changes of hippocampal and parietal P3-like after the inhibition of acetylcholine activity in the septo-hippocampal pathway by intraperitoneal injection of atropine (30 mg/kg) were also detected. (4) The changes of conditional active avoidance response was tested by shuttle-box following forebrain ischemia/reperfusion. RESULTS: (1) The peak latencies of hippocampal and parietal P3-like were prolonged after forebrain ischemia/reperfusion. (2) The polarity of hippocampal P3-like were reversed after the inhibition of acetylcholine activity in the septo-hippocampal pathway by atropine. (3) The prolongation of P3-like peak latency was closely related to the lowering of the score of conditional active avoidance response after forebrain ischemia/reperfusion. CONCLUSION: (1) The formation of P3-like depends on the integrity of hippocampus formation. (2) Hippocampus is not the only generator of P3-like, P3-like could be produced in multiple way. (3) The activity of acetylcholine in the septo-hippocampal pathway has notable effects on the formation of P3-like. (4) P3-like could be used as the sensitive marker of cognitive function.

There are a number of potassium channels on the cerebrovascular smooth muscle cell membrane, generally they are divided to 4 categories: voltage-gated potassium channels, calcium-activated potassium channels, inward rectifier potassium channels and ATP sensitive potassium channels. They can modulate cerebrovascular tone, so as to impact on cerebral blood flow to adapt to different situations of physiological pathology. The structure and function of potassium channels change after subarachnoid hemorrhage, These changes may be associated with the occurrence and development of cerebral vasospasm. The application of potassium channel opener may relax cerebrovascular smooth muscle and attenuate cerebral vasospasm.

Objective To compare the treatment effects of catheter directed thrombolysis and thrombetomy for acute ar?terial ischemia of the lower extremity. Methods Patients who visited Tianjin Medical University General Hospital from 2011 to 2013 and were attacked by acute ischemic disease of lower extremities within 3 days (n=66) were investigated. Thrombetomy and thrombolysis treatment had been performed to 32 cases and 34 cases respectively according to patients’ own willingness. Postoperative symptom remission rate, limb salvage rate, fatality rate, reperfusion injury rate (including re?nal inadequacy, heart failure, muscle fascia compartment syndrome), bleeding rate and limb dysfunction rate were compared between these two groups. Results There was no statistical difference on the rate of symptom remission (93.8% vs 94.1%), limb salvage(96.9%vs 100%), case fatality rate(3.1%vs 0), bleeding rate(0 vs 8.8%)and limb dysfunction rate (0 vs 5.9%)between these two groups. However, the incidence of reperfusion injury was higher in the group of thrombectomy (37.5%vs 2.9%, P<0.05)compared to that of the other group. Conclusion Similar effect of treatment and less reperfusion injury, especially renal injury, had been achieved by CDT compared with thrombetomy.

Objective To investigate the incidence of drug resistance among new and retreatment TB patients from special hospital.Methods Totally 500 smear positive TB patients from June 2013 to December 2014 in Beijing Chest Hospital were enrolled.Phenotypic susceptibilities to cultured isolates were analyzed in 15 anti tuberculosis drugs by MGIT 960,include Isoniazid (INH),Rifampicin (RFP),Streptomycin (STR),Ethambuto (EMB),Kanamycin (Km),Amikacin (Am),Capreomycin (Cm),Ofloxacin (Ofx),Levofloxacin (Lfx),Moxifloxacin (Mfx),Paza-aminosalicylate (PAS),Protionamide (Pto),Linezolid (Lzd),Ethionamide (Eto),Pyrazinamide (PZA).Results A total of 500 TB cases were enrolled.71 samples among these were NTM infection and 12 samples were contaminted.The rest of 417 of cases infected with Mycobacteriuma,and the rate of drug resistance was 47.2％ (192/417) and the MDR rate was 28.2％ (120/417).The retreatment was significantly higher than that of the new in any drug resistance rate and MDR rate (P =0.000).100 of the retreatment isolates and 50 of the new isolates with Mycobacteriuma were selected to do the drug susceptibility test in 11 subsequent anti tuberculosis drugs (include:PZA,Am,Km,Cm,Ofx,Lfx,Mfx,PAS,Pto,Lzd,Eto).Five cases were contaminated,and in the rest of the cases,48 was the new and 97was the retreatment.The rate of the drug resistance to PZA,Am,Km,Cm,Ofx,Lfx,Mfx for the retreatment were significantly higher than the new (P ＜ 0.05).The rate of drug resistance to PAS,Pto,Lzd and Eto for the new and reteartment were no markedly differential in statistics.Conclusion This study further confirmed the rate of drug resistance in retreatment cases is higher,and the management of tuberculosis patients should be further strengthened.

Wireless Local Area Networks has been used increasingly in recent years. The technology is mainly used in a-cademia (such as campus)、medical areas 、 manufacturing 、 Storage industry 、 public area、serving industry ,and so on. And the related technologies are making progress all the time. This article analyzes the development of related standard 、the structures 、security and other descriptions of Wireless Local Area Networks, paying attention to the uses of the technology in medical areas .

Objective To observe the effect of acupuncture on post-stroke belching. Method Ninety-six patients with post-stroke belching were randomized into a control group and a treatment group by random number table, 48 cases in each group. The control group was intervened by conventional Western medication, while the treatment group was by acupuncture in addition to the medication given to the control group. The blood Cl﹣ and Ca2﹢ contents, as well as the symptoms score and occurrence of adverse reactions were compared before and after treatment. Result After intervention, the blood Cl﹣ and Ca2﹢ contents increased and the symptoms score decreased to different extent in both groups; except for the blood Ca2﹢ content, there were significant differences in comparing all the parameters between the two groups after intervention (P<0.05); there was a significant difference in comparing the occurrence of adverse reactions between the two groups (P<0.05), and there were less adverse reactions in the treatment group. Conclusion Acupuncture can effectively control the post-stroke belching and reduce the happening of adverse reactions.

Objective:To detect differences in results of routine biochemical indexesand serum anticoagulant heparin, explore the feasibility of plasma heparin in the emergency biochemical test in.Methods: Two hundred cases of patients in the emergency department of our hospital as the research object, peripheral venous blood samples, were installed on the ordinary drying tube and a heparin plasma in vitro, determination of 10 emergency patients in serum and plasma biochemical items using biochemical analyzer, comparative analysis of the effect of heparin plasmabiochemical test results, and to compare the two groups report time.Results: The difference of serum group and Mg2+ group, Cl-, plasma Ca2+, Cr, BUN, UA, ALT, GLU and other data is not obvious, no statistical significance, The plasma concentration of K+ group was significantly lower than serum group, serum concentration of Na+ was higher than group difference was significant, with statistical significance(t=8.167,t=4.361;P<0.05). Plasma group reported significantly less than the serum group(t=22.94,P<0.05).Conclusion: heparin sodium does not dissolve in the blood, with a strong ability for anticoagulation, emergency biochemical test can avoid the effect of blood coagulation test results, and shorten the time for patients toreport, to win the precious time, attention is needed to correct potassium ionconcentration difference test.

Objectives To establish a sensitive and special method for the detection of Ureaplasma urealyticum(Uu) using PCR microplate hybridization (PCR MPH). Methods A primer of ureasea gene was labeled by biotin. The amplification product was captured on streptavidin coated microplates, then products were quantified by hybridization with a digoxigenin labeled internal oligonucleotide probe. After revelation with an anti digoxigenin alkaline phosphatase coupled antibody(anti DIG AP), the amount was determined by optical reading. At the same time, PCR MPH was compared with Bio Merieux Mycosplasma IST. Results A method of PCR MPH for detecting Uu DNA was established. The morbidity among three groups for detecting 158 clinical samples was analysed. 65 were detected by PCR MPH and 56 by culture.Conclusion The results showed that this assay is rapid, sensitive, specific, and accurate, and is of value in clinical therapy.