Background and purpose: MicroRNAs are 19–25-nucleotide noncoding RNA molecules that regulate gene expression at the level of transcription and translation. The study aimed to confirm whether miR-216a suppresses cell proliferation and invasion by targeting PRKCA, thus to reveal molecular mechanism that miR-216a functions as a tumor suppressor in glioma. Methods: PRKCA 3’ untranslated region (UTR)-luciferase vector was constructed and dual-luciferase reporter gene assay was employed to examine the effect of miR-216a on luciferase activity. U251 cells were transfected with miR-216a mimics, and next Western blotting was performed to detect the expression of PRKCA protein. The effects of PRKCA downregulation on cell proliferation and invasion were observed after PRKCA siRNA was transfected into U251 cells. U251 cell proliferation assays were performed when cotransfected with miR-216a mimics. Results:The result demonstrated miR-216a could bind to the 3’UTR of PRKCA and inhibited the luciferase activity by 41%. PRKCA protein expressions were significantly down-regulated when miR-216a was overexpressed in U251. siRNA-mediated downregulation of PRKCA could suppress the potentials of cell proliferation and invasion. Conclusion:miR-216a suppresses cell proliferation and invasion by targeting PRKCA in glioma.

Objective To observe changes of ?-glycuronida se (?-G) activity in blood and intraabdominal exudate in a monkey model with ac ute necrotic pancreatitis and the effects of somatostatin.Method The ANP model was induced by intrapancreatic duct injection of bile and 5% trypsin. Somatostatin was administrated at a dosage of 10 ?g?kg~-1?h~-1 in the treatment group (6 monkeys), w hile normal saline was given in 6 control monkeys. Blood and exudate were col lected for the meas urement of ?-G activity (?g/h?ml~-1). Resul ts (1)the mortality rate was 100% and 50% in the control group and treatment group, and the average survival time was (31?9) h and (53 ?24) h (P

Child ; China ; Humans ; Rheumatic Diseases

Objective: To evaluate the result of distraction osteogenesis in patients with pretnaxillary deficiency using a self-made tooth-borne distraction device. Methods: A self-made tooth-bome distraction device was used for advancement of the maxillary anterior segment in 28 patients. Results; 26 cases were corrected satisfactorily and have no any complications. In 2 cases the distraction device did not work during distraction. Conclusion: This device can improve the soft tissue profile and correct the space deficiency of the maxilla.

Objective To investigate the control status of glycosylated hemoglobin (HbA1c) in the middle aged and elderly patients with type 2 diabetes mellitus (T2DM) in Lanzhou communities and analyze its influencing factors.Methods A total of 1 120 individuals having diagnosed T2DM aged 40-75 years in Lanzhou communities were selected.Questionnaire was conducted.Height,weight,blood pressure and lipid were examined.Body mass index (BM1) was calculated.The index were analyzed.Results The patients with HbA1c less than 7.0％ were 453 cases (40.4％,453/1 120),and HbA1c more than or equal to 7.0％ were 667cases (59.6％,667/1 120).The target rate of HbA1c in the patients receiving life style,one oral antidiabetic drug,more oral antidiabetic drug,insulin therapy were 49.2％(94/191),45.7％(182/398),41.5％(88/212) and 27.9％(89/319),respectively.The target rate of HbA1c were 38.6％(192/298),32.2％(49/152),32.7％(49/150),27.0％(85/315) in the patients overweight or obese,with macro or micro vascular complication,and 60.0％ (12/20),44.4％(200/450),41.6％(404/970),45.7％(368/805) in the patients without overweight or obese and macro or micro vascular complication,and there were significant differences (P ＜ 0.01 or ＜ 0.05).Multivariate Logistic regression analysis showed that the extension duration of diabetes,increased BMI,combining with macro or micro vascular complication and gradual strengthening antidiabetic treatment were independent risk factors for HbA1c target (P ＜ 0.05).Conclusions Current status of HbA1c control in the middle aged and elderly patients with T2DM in Lanzhou communities is not optimistic,which is affected by many factors,suggesting that professional education on management should be further strengthened.And optimal treatment programs and individualized comprehensive treatment are needed for most patients to improve glycemic control.

AIM:To observe the postoperative effect of the application of expanded artery island skin flap on the treatment of injuries of face.METHODS:Totally 20 patients,who were treated with expanded artery island flap transplantation for face injury at Cosmetic and Plastic Institute,Weifang Medical College,were selected between January 1997 and September 2004.Patients knew the trial and agreed to participate in it.All patients received two phases operation:During the first period of the treatment,according to the different characteristics and requirements of the patients,suitable expander was implanted and water was input regularly.During the second period,the expanded island skin flap was used to cure the injuries on the face or do organ reconstruction.The skin flap was 2.5 cm? 3.5 cm-5.0 cm?7.5 cm,and vessel pedicle was 12.5 cm at most.Follow up was done regularly after operation.RESULTS:A total of 20 patients were involved in the result analysis,no drop out.Follow-up was done for 6-24 months in 20 patients.There were 4 cases of at least 6 months and less than 12 months,7 cases of at least 12 months and less than 18 months,9 cases of at least 18 months and less than 24 months.After expandor implantation in the first period,the expanded skin received skin repair or organ reconstruction in the second period.The healing of the injuries was good and scars of the skin and organ displacement were not clearly seen.Their function was recovered well and shape was ameliorated.CONCLUSION:The expanded island skin flap can provide comparatively large-region flap and long vessel pedicle to increase the angle and flexibility of flap rotation,and make the repair looks closer to the normal area.

BACKGROUND: SSh as a Hedgehog signal protein can promote bone development, growth and remodeling.OBJECTIVE: To investigate the effect of bone marrow mesenchymal stem cells (BMSCs) combined with Shh modified nano-hydroxyapatite/collagen (nHAC) in the repair of femoral defects in rats.METHODS: Forty-eight Sprague-Dawley rats were randomly divided into four groups, and the model of femoral defects was established in these rats. At 14 days after modeling, experimental group was implanted with the BMSCs/Shh modified nHAC, scaffold group was implanted with simple nHAC, cell scaffold group was implanted with BMSCs/nHAC,and blank control group was without any implantation. At 3, 6, 9, 12 weeks after repair, X-ray examination, bone density measurement and bone biopsy in bone defect area were performed.RESULTS AND CONCLUSION: (1) X-ray examination: The Lane-Sandhu X-ray score and bone mineral density value in the experimental group at different time points after operation were significantly higher than those in the other three groups (P < 0.05). (2) Hematoxylin-eosin staining: 12 weeks after repair, a small amount of bone tissues but no bone marrow formed in the scaffold group; a small amount of bone tissues with absence of bone marrow formed in the cell scaffold group, and the residual scaffold was visible; in the experimental group, the scaffold was completely absorbed,and mature bone and medullary cavity formed with presence of bone marrow. (3) Scanning electron microscope observation: 12 weeks after repair, irregular arrangement of bone fibers and a large number of bone fossae were observed in the scaffold group; the cell scaffold group showed a large number of osteoblasts, but bone fibers still arranged irregularly; in the presence of the Haversian system, a large number of regularly arranged bone trabeculae were detective in the experimental group. These results elucidate that the Shh modified nHAC/BMSCs complex can promote the repair of bone defects.

Objective To carried out rat as deep inferior epigastic perforator (DIEP) flap necrosis model,to reveal the continuous blood fluid change in process of flap necrosis.Methods Seven SpragueDawley rats of male were used.The designed DIEP flap model was pedicled on the right-sided cranial perforator.Near-infrared fluorescent angiography was performed using SPY imaging system pre-and-aft operation and all angiography videos were compared and analyzed.Results Using SPY imaging system could observe the process of the blood fluid scattered from perforator through choke anastomoses to next vascular territories in living body.The study could clearly observe sequence change of blood fluid pr-and-aft operation,which could give the important information in revealing the reason of necrosis in DIEP flap.Cross-midline traffic vascular sparse and eventually could no longer wear through the contralateral abdominal wall vessel area and open in the region of lateral thoracic vascular occlusion of blood vessels to reach the flap to the far left side,and showed no blood flow in the dark zone.Conclusion The reasons of necrosis of contralateral distal zone of the flap model,are the choke anastomoses across the midline to the contralateral side appeared small,less intensity and the blood supply to contralatral distal zone should through twice choke anastomoses.

Objective To investigate the value of acoustic radiation force impulse (ARFI)and color Doppler flow imaging (CDFI)in the diagnostic of acute rejection (AR)after liver transplantation.Methods B mode ultrasound,CDFI and ARFI exams were performed on fifty-eight patients who was diagnosed with AR through liver biopsy in our hospital.The liver allograft size,mean velocity of portal vein,hepatic vein spectrum waveform and shear-wave velocity were obtained and compared with the results of 30 healthy subjects as control group.Results Compare to control group,the AR group had a significant increase in liver allograft,decrease in mean velocity of portal vein,the elimination of triphasic wave of hepatic vein,and a significant increase in shear-wave velocity (P =0.000,0.000,0.007,0.000,respectively).The correlation coefficient between four criteria and grouping was 0.478,0.557,0.286,0.483,respectively.The area under ROC curves for diagnosing AR using the four criterias mentioned above was 0.914.Conclusions Combination of CDFI and ARFI in diagnosing of AR after liver transplantation has higher sensitivity and accuracy.

Objective:To construct and express the fusion protein between ZZ affinity peptide and alkaline phosphatase and examine its biological activities.Methods:The alkaline phosphatase gene was cloned into pEZZ 18 vector containing ZZ peptide gene resulted in the pEZZ-AP recombinant vector.Then the vector was transformed and expressed in E.coli DH5α.And HisTrap affinity chromatography was employed to separate and purify the target protein.After analyzed by Western blot , ZZ-AP fusion protein was applied to immunocytochemistry as an alterative second antibody.Results:The result of SDS-PAGE showed that the fusion protein with a relative molecular weight was consistent with the theoretical values (62 kD).The fusion protein has rabbit IgG-binding ability and en-zymatic activity of alkaline phosphatase ,those were validated in Western blot;and it produced a good signal that was comparable in its staining pattern to that generated with goat anti-rabbit IgG-AP in immunocytochemistry.Conclusion: The ZZ-AP fusion protein was constructed successfully ,it has rabbit IgG-binding ability and enzymatic activity of alkaline phosphatase.We anticipate that the ZZ-AP fusion protein has a potential application in immunoassay field.