Objective To observe the influence of clopidogrel combined with proton pump inhibitor (PPI) on the postoperative complication of percutaneous coronary intervention.Methods The clinical data of 600 patients who were taking clopidogrel antiplatelet therapy were retrospectively analyzed.All patients were divided into observation group (with PPI,n =300) and control group (without PPI,n=300).In-stent restenosis (ISR) and the adverse event rates were compared between the two groups during the period of follow up.Results The incidence of gastrointestinal bleeding was less in observation group than in control group (6.7％ vs.13.7％,x2 =8.048,P＜0.01),but the incidences of ISR,target vessel revascularization (TVR) and non-fatal myocardial infarction were higher in observation group than in control group(x2 =6.426、5.511、4.718,all P＜0.05).Conclusions Clopidogrel combined with PPI treatment can significantly reduce the risk of gastrointestinal bleeding,but increase the incidences of major adverse cardiovascular events.We suggest that PPI should be used with caution for the patients who need longer-term PPI use.

Objective To investigate the expressions of interluekin-3 in BALB/c mice infected by mycoplasma pneumonia (MP)and to clarify the relationship between IL-3 and MP infection.Methods Total 36 BALB/c mice were randomly divid-ed into control group,MP infection group and clarithromycin treatment group.MP infection models were established with the method of dripping cultured MP into nasal cavity of mice.ELISA method was used to assay the levels of IL-3 in the the serum of the mice and bronchoalveolar lavage fluid in various groups.The lung tissue was fixed in formalin for sections,HE staining was performed for the observation of histological changes.Results The MP-infected mouse models were established successfully and MP strains were dripped into the respiratory system of mice.As showed in histopathological observasion re-sults,significantly increased after MP infection and then gradually decreased after clarithromycin treatment.The ELISA re-sults showed that the level of serum and bronchoalveolar lavage fluid of IL-3 of the mice in MP infection group were 96.4± 8.3 ng/L and 60.9 ±6.5 ng/L.Compared with the control group(203.1±14.5 ng/L,96.3±12.6ng/L),serum and bron-choalveolar lavage fluid of mice infected with MP in IL-3 levels were significantly lower (t serum = 19.50,t balf = 17.20,P <0.01),and after treatment with clarithromycin,serum and bronchoalveolar lavage fluid of IL-3 significantly increased,re-spectively(175.6±12.5 ng/L and 85.6± 10.2 ng/L,tserum = 17.41,tbalf = 14.62,P <0.01),the difference was statistically significant.Conclusion IL-3 levels were decreased in mycoplasma pneumonia mice.Clarithromycin treatment had effect on mycoplasma pneumonia infecttion.The expression level IL-3 may be related to the severity degree of MP infection.

Aim To observe the effect of DL-buthionine s ulfoximine(DL-BSO) on the radiosensitivity of rat C 6 glioma cells under the a erobic and the hypoxic condition. Methods The source of radiati on was 60Co ?-rays. The rats C 6 glioma cells were treated by radia tion alone or DL-BSO+radiation under the aerobic and the hypoxic condition. Col ony forming assay was used to measure effects of DL-BSO on the radiosensitivity . Results Radiosensitive effect of DL-BSO was time-depedent u nder the aerobic condition. After treatment with 0.1 mmol?L -1 DL-BSO fo r 2, 6, 12 hours, the radiosensitive effect was not observed, whereas an enhance ment of radiosensitivity was seen at 24 and 48 hours. An enhancement of radiosen sitivity was seen at 2～48 hours after treatment with 0.1 mmol?L -1 DL-B SO under the hypoxic condition. The radiosensitive effects related to DL-BSO co ncentration under the aerobic and the hypoxic condition. Conclusion Both under the aerobic and the hypoxic conditions DL-BSO can increase the radio sensitivity of rat C 6 glioma cells. DL-BSO increased the rat C 6 gliom a cells radiosensitivity especially under the hypoxic condition, and radiosensit ive effect of DL-BSO is time and concentration-dependent.

Thrombolytic therapy for ischemic stroke has received wide attention. Now the thrombolytic therapy mainly includes intervenous thrombolysis, intra-arterial thrombolysis, combination of intravenous and intra-arterial thrombolysis, and mechanical thrombolysis. The review mainly presents the recent progress in this field.

"Yongling"oral liquid composed of Cordyceps miliaris and Ganoderma lucidum was prepared by biochemical means. By comparing three different formulations,it was found that the optimum ratio between C. miliaris and G. lucidum was 7:3,which presented the entire active principles in proper proportion while retaining the original natural color and flavor.The preparation is a high protein,low fat,nontoxic oral liquid with diversified therapeutic actions and devoid of side effects

Objective To study the reverse roles of reverse effect of tetramethylpyrazine on the resistance to adriamycin in human ovarian carcinoma cell line A2780/ADM.Methods The drug sensitivity of A2780/ADM cells was measured by MTT assay.The intracellular concentration of ADM was determined by fluorescent spectrophotometry.The changes of cell apoptosis percentage of A2780/ADM was demonstrated by flow cytometry.Results The IC50 value of TMP in A2780/ADM cell line was significantly decreased by the non-cytotoxic dose or low-cytotoxic dose of TMP.The resistance reversing effect comparing with the IC50 value of ADM cells was respectively 2.07 folds.The intracellular ADM concentration in A2780/ADM cells was significantly elevated by TMP at the same dose and the cell apoptosis percentage was significantly increased by TMP.The expression of P-gp was reduced significantly by TMP.Conclusion TMP could reverse the resistance of human ovarian carcinoma of A2780/ADM cell line to ADM,which may be related to the increased intracellular.

Objective:To determine the expansion and differentiation effect of Staurosporine on human megalaryoblastic cell line(CMK).Methods:With MTT and colony assaies.Results:Staurosporine could densitily inhibited the expansion of CMK cell,IC 50 was 50 nmol/L,Staurosporine could inhibite CMK cell at G 1 phase,then decreased the cells of S and G 2M phases,ST in 80 nmol/L improve the expression of CD41 antigen in CMK cell,induce its differentiation.Conclusion:ST as a new kind of anti-tumor facor has different mechanism with other anti-tumor drugs. [

Nephrotic syndrome,a common glomerular disease in pediatric department,is characterized by its long course of disease and facility to repeat or relapse,thus severely influences the physical and mental health of pediatric patients.Besides physical pains,most family members and patients show different degrees of mental stress towards this specific disease.Based on investigating clinical cases,we explored the features of mental stress among children with nephritic syndrome and their family members,and came up with corresponding psychological interference by effective communication skills between doctors and children with nephritic syndrome,offering humane concern,and proper instruction in clinical treatment in order to increase the compliance of children patients and their family members to medical treatment.Therefore,children patients and their family members manifest favorable attitude and cooperate with medical staff in the treating process,thus a satisfactory treating outcome will be achieved.

Objective To assess the life cycle, focus of research and developments of transcranial magnetic stimulation (TMS) from the perspective of technology management and information research.Methods Based on data sources from the Web of Science Core Collection and Derwent Innovations Index,such research methods as statistical analysis and social network analysis were adopted.The whole dataset was loaded into Bicomb, Loglet Lab and UCINET for further bibliometric analysis and network drawing.Results and Conclusion TMS research is currently quite active.The technology trajectory is in the second phase, having developed from the stage of growth to the stage of maturity.Network structure of TMS is taking shape, and there is still the lack of core technology themes.The present research focus of TMS will provide strong and firm support for its future medical application.In the next phase, research on the mechanism and neurobiological effects of TMS will be a hot spot.For practical assessment of specific technology, we should strengthen data mining and analysis, while bringing in the expert consultation method.

Objective To investigate the expression of ACTH in normal and hyperplastic adrenal cortical tissue, and to explore the relationship between plasma ACTH concentration and expression level of ACTH in adrenal cortical tissue of patients with adrenal cortical hyperplasia. Methods The expression of ACTH in adrenal cortical tissue was examined by immunohistochemical technique, and the result was analyzed by computer-assisted image analyzing system. Besides, plasma ACTH concentration was measured by RIA. The correlation between plasma ACTH concentration and expression level of ACTH in adrenal cortical tissue of patients with adrenal cortical hyperplasia was studied. Results The expression of ACTH was significantly higher in adrenal cortical tissue of patients with ACTH-independent adrenal cortical hyperplasia than those with ACTH-dependent adrenal cortical hyperplasia and normal adrenocortical tissue (both P0.05). Conclusion ACTH expression exists in normal adrenal cortical tissue. Local ACTH overproduction appears to participate in the pathogenesis of ACTH-independent adrenal cortical hyperplasia.The raised concentration of plasma ACTH influences the expression of ACTH in cortical tissue of patients with ACTH-dependent adrenal hyperplasia.