With the understanding of the function mechanism of RNAi,it has been widely applied in various fields.RNAi is a powerful tool to study gene functions and provides a new technical method for the specific gene treatment as well.The further development of RNAi technique opens up a new way for tumor gene treatment.

Objective To analyze the sequence of the novel conjugative plasmid pO157_Sal detected in outbreak isolates of Escherichia coli O157∶H7 .Methods The traE genes of the outbreak isolates in China were amplified by polymerase chain reaction (PCR) and the products were sequenced .The TraE sequences of Escherichia coli O157 ∶ H7 strains from other sources were retrieved from GenBank . Phylogenetic tree based on the TraE sequences was constructed by Neibhor-joining analysis .The whole plasmid sequences of pO157_Sal and pEC4115 were compared .Results The sequences of traE gene were identical among the Chinese isolates . There were homologous sequences of TraE in Escherichia coli O157∶H7 isolates from different sources .Twenty-one out of the 52 pO157_Sal genes were homologous to genes of pEC4115 with amino acid level identity ranging from 28% to 51% .Conclusions Although similar TraE sequences and similar plasmid are found in Escherichia coli O157∶H7 isolates from different sources ,pO157_Sal is only observed in Chinese outbreak isolates .The TraE sequences are conservative among the outbreak isolates ,indicating they are from the same specific source .

BACKGROUND:With the development of tissue engineering technology, repairing large-area bone defects using tissue-engineered bone has become a hot spot.
OBJECTIVE:To introduce the bone tissue engineering seed cel s, cytokines, as wel as the characteristics of scaffold materials and their vascularization.
METHODS:With the key words of“bone tissue engineering, scaffold, vascularization”in Chinese and in English, respectively, a computer-based search of articles published from January 2000 to January 2012 was performed in CNKI and PubMed databases. Articles with the summary of bone tissue engineering, bone tissue engineering scaffolds and scaffold vascularization were included.
RESULTS AND CONCLUSION:The selection of seed cel s, application of cytokines, scaffold material performance and degree of vascularization in bone tissue engineering has an important influence on the repair of bone injuries. Appropriate seed cel s is the study foundation in bone tissue engineering, cytokines serve as catalysts, and scaffold materials with good three-dimensional structure can promote cel growth and proliferation, tissue ingrowth, osteogenesis and vascularization. Each scaffold has its own inadequacies, so the combination of a variety of materials can reach a combined effect to meet the clinical demand. In addition, it is important to actively seek new material preparation technology and improve the existing methods, in order to create a more excel ent scaffold. But the vascularization is stil a major test for bone tissue engineering. Current methods to promote vascularization of tissue-engineered bone have some defects. For examples, the use of growth factors to promote vascularization can lead to disease progression in patients with metabolic abnormalities during;microsurgical techniques for tissue engineering bone vascularization are easy to cause trauma and deformity at other parts, which is not conducive to the patient’s physical rehabilitation.

Objective To compare the renal effects of dopamine and dobutamine in patients with sepsis.Methods 90 patients with sepsis were admitted to this study.After resuscitation,each patient was randomly given different vasoactive agent.The changes in urine output,fractional excretion of sodium(FeNa),and creatinine clearance(CCr)were observed.Results The urine output and FeNa in dopamine group were increased significantly as compared with control group and dobutamine group[(3072±480),(2038±515)and(362±522)ml/24h,(3.80±1.09),(2.06±1.14)and(2.10±0.95)%](P＜0.05).Compared with control group and dopamine group,CCr increased significantly in dobutamine group[(79.2±39.1),(50.6±21.8)and(47.4±16.7)ml/min](P＜0.05).Conclusion Dopamine infusion markedly elevates urine output and FeNa,but has no effect on CCr.Dobutamine treatment misht significantly increase CCr,but has no effect on urine output.

Objective To study the effects of treating irritable bowel syndrome (IBS) with modified TongXieYaoFang. Methods 83 IBS patients were randomly recruited into a treatment group (45 patients) and a control group (38 patients). In the control group, Trimebutine, 200mg, and Bifid triple viable capsule, 420mg, 3 times/day was given orally to the patients, while on the basis of that, the treatment group was administrated with modified TongXieYaoFang in addition. Both groups were treated for one course of 4 weeks. Results The total effective rate of the treatment group and the control group was 93.3% and 71.1% respectively, showing significant difference between the two groups (X2=7.2938, P<0.01 ). Conclusion TongXieYaoFang combined with western medicine is effective in treating IBS.

Objective To explore the clinical characteristics of multiple primary carcinoma (MPC) in elderly patients and the effects of postoperative adjuvant chemotherapy on the prognosis and advanced cancer genesis. Methods The study population consisted of 220 elderly patients with malignant tumor. Thirty-seven elderly patients with MPC were selected in the study. The characteristics including onset age, carcinoma sites, effects of postoperative adjuvant chemotherapy, interval and survival time between first and advanced cancer were analyzed. Results of the 220 subjects,37(16.8%) patients were MPC, among whom 2 patients were synchronous multiple primary carcinomas (SMPC) and 35 patients were metachronous multiple primary carcinomas(MMPC). The median ages at onset were 70,77 and 77.5 years old for the first,second and third tumor respectively. Among a total of 82 tumor focus, the most predilection site of MPC was gastrointestinal tract, with a 34.2%(28 cases) in colorectum and a 13.4%(11 cases) in stomach. The postoperative patients were divided into two groups: 23 accepted adjuvant chemotherapy (chemotherapy group) and 14 accepted no adjuvant chemotherapy (no chemotherapy group). The survival time of chemotherapy group was significantly longer than no chemotherapy group (x2= 4.63, P = 0.0427 ). The median intervals between the first and the second cancer were 6 years and 4 years in chemotherapy group and no chemotherapy group, respectively(x2=1.63, P = 0.207), while the median intervals between the second and the third cancer were 7 years and 2 years, respectively(x2 = 4.255,P=0.043). The mediansurvival time of the third cancer postoperative patients were 3.5 years and 7 months in chemotherapy group and no chemotherapy group, respectively (x2 = 4.62, P = 0.0316 ) . ConclusionsPostoperative adjuvant chemotherapy may prolong the survival time and delay the advanced cancer genesis in elderly patients with MPC.

Thrombelastography (TEG) is an image presenting the viscoelasticity changes of blood clot in the process of blood clotting,consequently reflecting the ability of blood clotting and fibrinolysis.TEG is increasingly used in the field of guiding intraoperative blood transfusion,monitoring the Blood coagulation function,treating trauma patient,and monitoring the blood coagulation and fibrinolytic function of a variety of diseases,due to its rapid and accurate results.We discuss the clinical applications progress of TEG in this article.

Objective To explore the TCM pathogenesis based on the analyses of TCM pattern elements. Methods TCM pattern elements of 84 patients with middle-late pancreatic cancer to analyze the TCM disease location and characteristics by their frequency and the contribution scores. Results A total of 84 patients were included, TCM disease location with the top 10 rank of the contribution scores were spleen, stomach, liver, gallbladder, kidney, intestine, lung, heart, spleen, stomach, while they were significantly higher than others (P<0.05). And the TCM pattern elements with the top 5 rank of the contribution scores were qi deficiency, dampness, heat, blood stasis, toxin, while they were significantly higher than others (P<0.01). Conclusions TCM disease locations of middle-late pancreatic cancer were mainly in spleen and stomach, and TCM pattern element were excessive patterns with qi deficiency, deficiency patterns with dampness, heat, blood stasis and toxin.

Objective The study aimed to explore the application of fast track surgery in nursing of patients with colon cancer in a primary hospital.Methods The 60 colon cancer patients were identified with numbers to indicate randomization into the fast tract surgery group (group A) and the conventional treatment group (group B) with 30 cases in each group.Group A used the concept of fast track surgery (FST) perioperative nursing care,whereas traditional perioperative nursing care for colon cancer was used in group B.Patients in both groups were observed for postoperative time for passage of gas by anus,time for first food intake,length of stay in hospital,fee for hospitalization as well as incidence of postoperative complications,and comparative analysis was carried out.Results Time for passage of gas by anus(t value was 3.571),time for first food intake (t value was 8.944),length of stay in hospital (t value was 3.397),fee for hospitalization (t value was 3.950) as well as incidence of postoperative complication in group A were lower than those of group B.The satisfaction degree of group A was 96.67％(29/30) while 83.33％(25/30) in group B and the satisfaction degree between the two groups was statistically significant.Conclusions In the primary hospital,the fast track surgery care used in colon cancer can not only speed up the recovery process of the patients,shorten hospital stay,reduce hospital cost and the incidence of postoperative complications,but also can reduce the workload of nurses and improve the satisfaction degree of patients.

Objective: to observe the effect of Roux-en-Y Gastric Bypass (RYGB)on STZ induced type 2 diabetic rat model, and to clarify its initial mechanism. Methods: 42 type 2 diabeticrats were randomly allocated into operation group(group O 12 rats); sham group(group S 10 rats); diet control group(group F 10 rats); control group(group C 10 rats). Fasting glucose, Insulin Growth Factor-1 ( IGF1), fasting plasma insulin levels, weight and food intake were measured before and 1st , 2nd, 3 rd ,4th, 8th and 16 th week after operation. Results: 16 weeks after operation in the Group O, fasting glucose fell to (8.8?4.9)mmol/L, IGF1 increase to (148.6?7.3)ng/L ,fasting plasma increase to (74.7?9.2)mIU/L and weight decline to (212.6?15.1)g ,there was statistical significance compared with the levels of preoperation (P