Objective To investigate the expression of serum interleukin-2(IL-2),tumor necrosis factor-α(TNF-α)in the pa-tients with pulmonary tuberculosis and diabetes,and prelininary explore its clinical significance.Methods Experiment were divided into control group(health check-up outpatient,n=10),pulmonary tuberculosis group(n=20),diabetes group(n=20),tuberculosis combine diabetes group(n=20),tuberculosis combine diabetes group was divided into improved group,diabetes group and deterio-rated group after 2 months with therapy according to disease outcome,ELISA assay was used to measured the level of serum IL-2 and TNF-α.Results Before treatment:serum IL-2 levels of pulmonary tuberculosis group,diabetes group and pulmonary tuberculo-sis combine diabetes group were reduced (P<0.05),and the pulmonary tuberculosis group was lower than the pulmonary tubercu-losis group and diabetes group (P<0.05);serum TNF-αlevels of pulmonary tuberculosis combine diabetes group,pulmonary tu-berculosis group and diabetes group increased (P<0.05),and that of pulmonary tuberculosis combine diabetes group was higher than that of diabetes group (P<0.05).After treatment:the level of serum IL-2 increased and TNF-αsignificantly decreased in pul-monary tuberculosis combine diabetes improved group (P<0.05),the deterioration group had no change(P>0.05).Conclusion IL-2 and TNF-αplay a role in the immune pathogenesis of tuberculosis combine diabetes,which dynamic changes can reflect the progression and outcome of pulmonary tuberculosis combine diabetes.

Presently, with the development of tissue engineering technology, medical workers have seen a more effective and safer therapy to repair and reconstruct tissue defects. Many domestic and abroad animal experiments have confirmed that it is feasible, effective and superior to use tissue-engineered bone and cartilage for repairing tissue defects. There have been achieved considerable results using tissue-engineered bone and cartilage to repair tissue defects and reconstruct tissue functions through the animal experiments and initial clinical studies. Compare with traditional operations, tissue engineering technology has a broader clinical application, especially in orthopedics, department of stomatology, cranio-facial surgery and ear, nose and throat, department. However, through the research, we recognize some deficiencies of tissue engineering, such as single tissue components, lack of biological activity, limited for scaffolds, tissue regeneration and productions.

Cardiomyopathies ; diagnosis ; Diagnostic Imaging ; Humans ; Ventricular Dysfunction, Left ; diagnosis

OBJECTIVE: To investigate the progress of clinical application of implant during distraction osteogenesis in the jaw bones, and to evaluate its biocompatibility.METHODS: The first author retrieved CNKI database from 1989 to 2009 in Chinese and Medline database from 1989 to 2009 in English with the key words of "jaw, distraction osteogenesis, treatment". A total of 56 articles were screened according to inclusion and exclusion criteria. Finally, 20 articles concerning treatment progress of distraction osteogenesis and clinical application of implant were included.RESULTS: Distraction osteogenesis implants contained intraoral or extraoral distraction device, which could be used for distraction of transport plate. A distraction was selected in accordance with patients' condition and requirement. Distraction osteogenesis had been an important manner for complicated tooth jaw facial deformity and jaw defect in oral dentofacial surgery and Orthodontic treatment. It could have effective osteogenesis in combination with various systemic therapy, local therapy and physical therapy. Presently, distraction osteogenesis implants were metal materials. Metal implants have great advantages in preventing bacterium growth, keeping implant fixation and distraction effects, and the price was high. However, the cost of Ni-Ti memory alloy wire was lower compared with common retractors. The fixing device and alloy wire closely adhered to the bone could be completely implanted into tissues, with the advantages of anti-infection and completely closing the exterior and interior wound. CONCLUSION: Distraction osteogenesis is a new method for treating dental and craniofacial hypodevelopment straightening of teeth and for repairing jaw bone defect deformation. Biocompatibility of metal implants was good such as internal small Ni-Ti memory alloy wire. The biocompatibility of other types of implants should be improved.

Coronary Angiography ; Coronary Disease ; diagnosis ; diagnostic imaging ; Diagnostic Imaging ; methods ; Humans

Objective To investigate the expressions of amyloid precursor protein and β-microtubule protein,and to study their clinical significance in patients with cerebral infarction.Methods 108 cases of acute cere-bral infarction as the observation group and 60 normal adults as the control group were collected in the study.The expressions of amyloid precursor protein and β-microtubule protein in different clinical characters were detected by western blot.Results The results of observation group [APP:(168.78 ±13.64)μg/L,β-tubulin:(124.64 ± 27.08)μg/L]were significantly higher than those in control group (t =22.81,P <0.01;t =21.08,P <0.01).Their expressions were correlated with CT scores and prognosis.Conclusion The higher expressions of amyloid precursor protein and β-microtubule protein may promote the occurrence and development in patients with cerebral infarction. The joint detection should be helpful to predict the prognosis and direct the treatment.

China ; Feeding Methods ; Female ; Humans ; Infant, Newborn ; Infant, Premature ; growth & development ; Infection ; complications ; therapy ; Obstetric Labor, Premature ; complications ; mortality ; Pregnancy ; Respiration, Artificial ; Respiratory Therapy ; Survival Rate

OBJECTIVE:To optimize the extraction of effective ingredients and purification for crude glycyrrhizic acid and liquiritin from Radix et Rhizoma Glycyrrhizae with different methods. METHODS:The contents and extraction percentages of glycyrrhizic acid and liquiritin were used as indexes to optimize the extraction solvent. Then the extraction process was optimized with orthogonal experiment taking the concentration of ammonium ethanol,the amount of solvent and the extracting times as indexes. Crude glycyrrhizic acid was purified with the method of recrystallization and crude liquiritin was purified with organic solvent method.RESULTS:The optimal extracting solvent was ammonium ethanol. The optimum extraction conditions were as follows:extracting the solvent 4 times(2 h/time) by adding 5 times volume of 60% ethanol(containing 0.3% ammonia water). The purity of glycyrrhizic acid was over 85% with total extraction rate at 43%,and content of liquiritin was above 15% with its total extraction rate at 67%. CONCLUSION:The method is simple and feasible and from which glycyrrhizic acid and liquiritin was been obtained,Radix et Rhizoma Glycyrrhizae can broaden the scope of the application.

Congenital central hypoventilation syndrome,also known as “Ondine′s curse”,is charac-terized by hypoventilation during sleep and impaired ventilatory responses to hypercapnia and hypoxemia. This disorder is associated with a malfunction of the nerves that control involuntary body functions and abnor-mal development of early embryonic cells that form the spinal cord. This review summarized the latest pro-gression in the pathogenesis,clinical symptoms,diagnosis,treatment and prognosis.