In recent years,direct-acting antiviral agents (DAAs) have achieved great success in the treatment of hepatitis C and have replaced interferon/ribavirin.However,since DAAs were launched not long ago,there lacks sufficient knowledge of their toxic and side effects,interactions with other drugs,and safety in patients complicated by other serious chronic diseases.The results of many large-scale clinical trials show that DAAs have good safety in different populations and serious toxic and side effects are rare,but drug interactions need to be taken seriously.The addition of ribavirin in DAA regimen or prolongation of DAA treatment does not increase patients' benefits and may cause more adverse events.Moreover,at the same time of DAA treatment,liver injury caused by HCV cannot be neglected,and continuous treatment should be given.

In the present study, A fluorescent imaging-based high-throughput screening method was developed for identifying anti-migratory compounds with 96-well Transwell plates. The correlation, precision and stability of this method were examined and the incubation time of dye Hoechst 33342 in addition to migration time was optimized. In addition, The inhibitory activity of anti-cancer drug paclitaxel on tumor cell migration was assayed and an IC50 value of 0.717 micromol x L(-1) was obtained. Using this method, 24 components from Rhizoma Alismatis were screened and one component with anti-migration activity was found. These results show that the new proposed method with good precision, stability and linear range has the potential to assay the inhibitory activity of anticancer compounds.

The pharmacokinetics of ketamine were studied in 8 preschool children,aged 3.5～6 years. The ketamine was administrated by I. V. infusion at a dose of 3mg?kg~(-1) in one hour constantly. The plasma concentrations of ketamine were measured by high performance liquid chromatography. The disposition of intravenous infusion with ketamine could be described by two-compartment open model, and the variables of pharmacokinetics of ketamine were as follows: T_(1/2?)=0.15?0.04 hours,T_(1/2?)=2.84?0.44 hours,Cl=0.34?0.06L?kg~(-1)?h~(-1), AUC=9.21?1.00mg/L?h, and V_d=0.66?0.29 L?kg~(-1). This suggests that intravenous infusion of ketamine at 3mg?kg~(-1)?h~(-1) is safe for the maintenance of general anesthesia in preschool children, attention must he paid to the children anesthetized with long period of ketamine infusion because recovery may be delayed due to the prolonged elimination half-life.

Development and metastasis of NSCLC are closely related to angiogenesis.Several distinct groups of vascular-targeted therapies have evolved:anti-VEGF monoclonal antibody,small molecules inhibitors,vascular disrupting agents,endothelial cell growth inhibitors,et al.Antiangiogenic treatment could make longer survival than conventional treatment.The strategy of anti-angiogenic drugs combined conventional treatment is being studied.

Objective To investigate the relationship between serum plasma brain natriuretic peptide ( BNP ) levels and myocardial injury in neoborns after asphyxia. Methods Neoborns who were admitted to Department of neonatology, Zhongnan Hospital, Wuhan University from December 2012 to December 2013 within 3 days after birth were considered. According to the number organized in chronological order every other case, newborns with neonatal asphyxia were assigned to observation group. The observation group were further divided into myocardial injury subgroup and non-cardiac injury subgroup according to the diagnostic criteria of myocardial injury. Newborns without neonatal asphyxia or neonatal cardiovascular diseases were assigned to control group. Exclusion criteria for control group were electrolyte disturbance, liver and kidney dysfunction. Blood sample was drawn from patients within 2 hours of admission to hospital and again on day 14. Serum BNP , creatine kinase isoenzyme ( CK-MB) , serum sodium and calcium were detected for further analysis. Results In 107 cases with neonatal asphyxia, 77 infants who had complete clinical records were selected as observation group, of which 36 met the diagnostic criteria of myocardial injury and assigned to myocardial injury subgroup. Non-cardiac injury subgroup consisted of the rest 41 cases in observation group. Twenty-two cases were enrolled to control group. Within 2 hours after admission, the serum BNP level of myocardial injury subgroup were significantly higher than those of the non-cardiac injury subgroup and the control group ( 2. 35 ± 0. 44 , 2. 12±0. 64, 1. 88±0. 27, log transformed, respectively, P＜0. 05). The BNP level of non-cardiac injury subgroup were also significantly higher than those of the control group. Serum BNP and CK-MB levels of observation group were positively correlated (r=0. 212,P=0. 030). After treatment, serum BNP level of myocardial injury subgroup at 14 days after admission decreased significantly, compared to the level at 2 hours within admission (P＜0. 05). When the cutoff value for infants with myocardial injury was 108. 05 pg/mL, the area under the ROC curve was 0. 753, with a sensitivity of 75. 0％ and a specificity of 64. 5％, positive predictive value was 56. 4％ and negative predictive value was 72. 3％. Conclusions Serum BNP level can reflect myocardial injury in neonates with asphyxia and can guide clinical treatment.

Objective To evaluate the prevalence rate of pulmonary malignant disease detected by low dose spiral CT in people at high risk of lung cancer. Methods Low dose spiral CT scans and chest radiographs in 300 symptom free volunteers from an on going screening study were prospectively evaluated. The study has enrolled 240 smokers, aged 45 years or older, with at least 10 pack years of cigarette smoking and 60 individuals with chronic obstructive pulmonary disease, and without previous cancer history, who were medically fit to undergo thoracic surgery. Low dose CT scans were performed with SR 7000 scanner using spiral mode, 120 kV, 50 mA, pitch 2, 5mm thickness reconstruction and Lightspeed Plus multi slice scanner using spiral mode, 120 kV, 50 mA, pitch 6 to produce 2.5 mm thick image at 2.5 mm increments. All images were assessed with cine display mode on workstation monitor. Results Non calcified nodules were detected in 56 (19%) participants by low dose CT, compared with 9 (3%) by chest radiography. Malignant disease was detected in 4 (1.3%) by CT and 3 (1%) by chest radiography. All 4 cancers were stage I. Lobar or segmental bronchial abnormalities were detected in 9 (3%) participants by CT. Among them, 3 (1%) proved to be early central lung cancer. No bronchial abnormality was detected by chest radiography. The sensitivity and specificity of cancer screening was 43% and 89%, respectively for chest radiograph, 100% and 80%, respectively for CT. The sensitivity of CT was significantly higher than that of radiograph, whereas the specificity showed no statistical difference. Conclusion Preliminary screening study indicates that low dose CT can greatly improve the likelihood of detection for small non calcified nodules and mild bronchial abnormalities, and thus of peripheral and central lung cancer at an earlier stage.

Objective To study the CT findings of early central lung cancer and correlative pathologic basis, and to evaluate the role of CT in the differential diagnosis of central lung cancer Methods Seventeen patients with early central lung cancer and 10 patients with benign lesions resembling the central lung cancer were analyzed Thin section CT was performed with a section thickness of 3 mm or 1 5 mm at Philips SR 7000 or GE Lightspeed Plus scanner The visibility of the lesions was correlated with bronchoscopic and histopathologic findings Results All 17 lesions were visualized at CT Three lesions showed focal bronchial wall thickening and internal bronchial wall irregularity, bronchial mucous coarsening, redness and swollening at bronchoscopy Fourteen lesions showed focal narrowing or obliteration of the bronchial lumen, 4 complicated with subsegmental bronchial mucoid impaction, 10 with obstructive pneumonia or atelectasis Corresponding bronchoscopic view disclosed intraluminal polypoid protrusions The epithelia were involved in 1 case, subepithelia in 5, and cartilaginous involvement in 11 according to the depth of invasion of the bronchial wall at histopathology Among 10 patients with benign lesions, 6 cases were suspected as bronchogenic carcinoma and the possibility of bronchogenic carcinoma was not excluded at CT in 4 cases There were 5 patients with uneven thickening of internal bronchial wall, 2 with lumen blood clots, 1 with lumen nodule, and no abnormalities in 2 patients at bronchoscopy Conclusion Thin section CT has been proved to be a reliable method for demonstrating the mild changes of the bronchi and to be a valuable tool for the diagnosis of early central lung cancer

0.05),there were more apoptosis-starting cells found in normal skin(1738.33?348.89/mm2)than in keloid(891.67?395.00/mm2)(t=-5.565,P=0.000).The number of positive stained fibroblast cells in dermis significantly increased in keloid group(911.67?323.61/mm2),compared with that in normal skin group(220.00?80.00/mm2)(t=7.188,P=0.000).This result that the protein expression level of Bid was higher in keloid(0.46?0.08)than that in normal skin tissue(0.02?0.01)(t=18.905,P=0.000)was proven by western blotting test.Conclusions The number of apoptosis-starting cells in epidermis of normal skin is more than that in keloid.Bid protein in dermis of keloid is over expressed.

0.05).The blood gas analysis(BGA) and pulmonary function test(PFT) were performed in patients with stable COPD.The cognitive function was evaluated by ESD in both patients with stable COPD and healthy persons as control.The patients were divided into different groups by BGA and PFT(FEV1/Pred).Results:The total score of ESD and the subtest scores of ESD in leaning,memory,calculation,constructive function were obviously lower in the patient group than those in the control group(The mean difference of the total score was 16,with the two groups' total scores as 208.1?17.6/224.3?10.6,t=5.19,P

0.05). No significant association between Q/R 192 genotype and blood lipids was found.Conclusion The polymorphism of PON-1 Q/R 192 gene is not associated with CI.