OBJECTIVE: To investigate the influence of aluminum hydroxide adjuvant on a murine model of allergic rhinitis (AR) and to confirm an appropriate method of establishing a mouse model of AR. METHOD: Establishing two types of BALB/c mice models of AR, one was identified as Local group which was characterized through intranasal sensitization and challenge using ovalbumin (OVA), and the other Systemic group which was made by intraperitoneal sensitization with OVA plus aluminum hydroxide and intranasal challenge through OVA. Then the numbers of sneezing and nasal rubbing were counted after the last challenge and the eosinophils in the nasal mucosa of mice models were observed and counted though Luna stain. Furthermore, morphological hyperplasia was examined in intraepithelial goblet cells and submucosal glands with HE stain. In addition, interlukin (IL) -4, IL-5, OVA specific IgE (sIgE) and interferon (IFN)-gamma in nasal lavage fluid (NLF) and serum of mice were examined u sing enzyme linked immunosorbent assay (ELISA). RESULT: The counts of sneezing and nasal rubbing in local group were more than those in systemic group and eosinophilia in the nasal mucosa of former group was greater than that in the latter one. Morphological hyperplasia was stronger in intraepithelial goblet cells and submucosal glands in local group compared with that in systemic group. Furthermore, the contents of IL-4, IL-5 and sIgE increased in the NLF and serum of mice of local group compared to those of systemic one. However, the production of IFN-gamma of mice in local group decreased when compared with that in Systemic group. CONCLUSION OVA plus aluminum hydroxide adjuvant may promote Th1 type immune response as well as Th2 response. OVA intranasal sensitization and challenge locally is an appropriate way in the establishment of AR mice models.
Adjuvants, Immunologic ; pharmacology ; Aluminum Hydroxide ; pharmacology ; Animals ; Disease Models, Animal ; Immunoglobulin E ; immunology ; Interferon-gamma ; immunology ; Interleukin-4 ; immunology ; Interleukin-5 ; immunology ; Male ; Mice ; Mice, Inbred BALB C ; Rhinitis, Allergic ; immunology

Benzene ; poisoning ; DNA Damage ; DNA Repair ; genetics ; Deoxyguanosine ; analogs & derivatives ; genetics ; Humans ; Mutagens ; poisoning ; Poisoning ; genetics

China ; epidemiology ; Disease Notification ; standards ; Epidemiologic Methods ; Humans ; Models, Statistical ; Occupational Diseases ; epidemiology ; prevention & control ; Occupational Health Services ; statistics & numerical data ; Registries ; Wounds and Injuries ; epidemiology ; etiology ; prevention & control

China ; Education, Medical ; Occupational Health

Objective To study and compare the present situation and the difference in subjective well-being between students in junior high schools for the blind and normal junior high school students, and provide basis for the construction of education mode for the well-being of the blind students. Methods By random sampling method, subjective well-being questionnaire was used to investigate the 155 blind students in five blind schools and 436 normal junior high school students in three junior high schools in Shandong Province. Results ( 1 ) The blind students had shown significant differences in many aspects of subjective well-being such as gender, family e-conomic status, sight and parents'education degree (P<0. 05) . (2) Blind school students'social confidence experience (4. 59 ±1.13) was significantly higher than that of the normal students (4.31 ±1.13), and had a significant difference (P<0.05). Blind students got significantly lower scores ((4. 38 ± 1.26) , (4.00 ± 1. 29) ,(3. 58 ± 1. 37) ,(3.89 ± 1. 35) ,(4.41 ± 1.04) , (4. 20 ± 1. 33) ) than normal school students( (4.68 ± 1. 19) , (4. 36 ±1.14),(3.88±1.27),(4.41 ±1.25),(4.61 ±0.99), (4. 52 ± 1. 18)) in target value experience, physical health experience, mental health experience, interpersonal adaptability experience self-acceptance experience, and emotional balance experience. Conclusion There exists a significant difference between the blind students and the normal junior high school students in the different aspects of subjective well-being.

Objective To investigate the changes of coagulation and fibrinolysis systems in patients with solid cancer. Determine the mechanism of thrombosis formation in solid cancer and the the metastasis mechanism of solid cancer.Methods To measure plasma concentrations of TF,TFPI,t-PA,u-PA,PAI-1 by ELISA and test protein C activity(PC:A)by chromogenic substrate assay.Results Plasma concentrations of TF,TFPI,u-PA,PAI-1 were all higher in solid cancer patients than normal control and higher in metasta- sized team than non-metastasized team.In the dead team,u-PA and PAI-1 were higher while TFPI was low- er. t-PA was higher in cancer patients combined with venous thromboembolism while protein C activity was lower.Conclusion Disorders of coagulation and fibrinolysis system are related to the thrombosis formation in solid cancer.Coagulation and fibrinolysis factors take part in the metastasis progress of solid cancer. High concentration of u-PA and PAI-1 or low concentration of TFPI are considered to be related with poor outcome.

OBJECTIVETo investigate the influence of normal saline nasal irrigation with different temperature on allergic rhinitis (AR) and to confirm the appropriate temperature of nasal irrigation.

METHODSSixty patients with AR were randomly divided into 3 groups (according to random number table) and received 15 °C, 25 °C or 40 °C normal saline nasal irrigation respectively, and 20 healthy controls received no treatment. Visual analogue scale ( VAS) was used to assess the symptoms among the control group and the various intervention groups and the enzyme linked immunosorbent assay (ELISA) was applied to detect the contents of histamine, leukotriene C4 (LTC4), house dust mite D1 specific IGE (sIGE) and eosinophil cationic protein (ECP) among the control group and the various intervention groups. GraphPad Prism 5 software was used to analyze the data.

RESULTSThere was no statistical difference between 15 °C normal saline nasal irrigation group and 25 °C normal saline nasal irrigation group. Pre-intervention and post intervention whether is VAS scores of rhinorrhea, sneezing, nasal obstruction and nasal pruritis or in contents of histamine, LTC4, sIgE and ECP showed no difference (all P > 0.05). However, the symptoms of (sneezing VAS score: 3.765 ± 0.291; nasal obstruction VAS score: 3.529 ± 0.365). The difference was significant (t = 4.678, P < 0.001; t = 3.901, P < 0.01). The contents of histamine [ (21.78 ± 0.62) ng/ml] and LTC4 (17.43 ± 0.67 ng/ml) were also decreased after intervention of 40 °C normal saline nasal irrigation [ histamine content: (18.82 ± 0.52) ng/ml; LTC4 content: (13.14 ± 0.59) ng/ml ]. The difference was significant (t = 3.632, P < 0.01; t = 4.79, P < 0.001). Nonetheless, there were no statistical differences in VAS scores of rhinorrhea (t = 2.02, P = 0.051) and nasal pruritis (t = 1.984, P =0.056) and in contents of sIgE (t = 1.891, P = 0.066) and ECP (t = 2.021, P = 0.05). There were statistical differences between the 40 °C group and the 25 °C group normal saline nasal irrigation group in symptoms of sneezing (t = 2.060, P = 0.048) and nasal obstruction (t = 2.1053, P = 0.048). and inflammatory factors of histamine (t = 2.078, P = 0.045) and LTC4 (t = 2.149, P = 0.038). In symptoms of rhinorrhea and nasal pruritis and local production of sIgE and ECP, there was no statistical difference (all P > 0.05).

CONCLUSION40 °C normal saline nasal irrigation can improve the symptoms of sneezing and nasal obstruction and can alleviate the local levels of inflammatory factors like histamine and LTC4 in AR patients, and is the appropriate irrigation temperature.

Adult ; Case-Control Studies ; Female ; Humans ; Male ; Middle Aged ; Nasal Lavage ; methods ; Rhinitis, Allergic ; therapy ; Sodium Chloride ; administration & dosage ; Temperature

In this study we implanted magnetically labeled neural stem cells (NSCs) in PD rats and then monitored their survival and migration in the host brain by magnetic resonance imaging (MRI). The mesencephalic NSCs were obtained from the brain of SD rats. Superparamagnetic iron oxide (SPIO) was transferred to NSCs by Lipofectamine transfection. Eighteen PD lesioned rats were selected for transplantation by evaluation of their rotational behavior in response to amphetamine and randomly assigned to 3 groups, i.e., sham group, PBS group and NSCs transplanted group, with 6 rats in each group. MR scanning was performed at 1, 2, 4, 6, 8 and 10 week(s) following transplantation. At the meantime, rotational behavior was assessed in each group. Our results showed that SPIO particles were clearly visible with Prissian blue staining in neurospheres and cells derived from NSCs. The rotational behavior of the NSCs transplanted group was remarkably improved compared with that of sham group and PBS group (P < 0.05). In vivo MR tracking of NSCs showed that SPIO labeling led to a strong susceptibility change of signal 1 week after transplantation on T2 weighted images. And a large circular hypointense signal appeared in the transplanted area on T2* gradient echo images. Ten weeks following transplantation, the hypointense signal on T2 weighted and T2* gradient echo images was still displayed. It is concluded that SPIO particles could label NSCs effectively, and MRI detection of SPIO labeled cells is a promising method and novel approach to analyzing the NSCs following transplantation in the treatment of PD.

Carcinogens ; Carcinogens, Environmental ; Retrospective Studies ; Threshold Limit Values

Acupuncture Points ; Acupuncture Therapy ; Adult ; Aged ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Male ; Middle Aged ; Stroke ; complications ; Urinary Retention ; drug therapy ; therapy