Cardiovascular Diseases ; etiology ; prevention & control ; Humans ; Metabolic Syndrome

Cardiovascular Diseases ; prevention & control ; China ; Humans

In order to investigate the mutation of HBV polymerase gene reverse transcription conserved region (P region) in chronic hepatitis B (CHB) patients, 212 CHB patients who took antiretroviral treatment with nucleotide analogues were chosen. The drug resistance mutations of HBV P region and HBV genotype were detected by Pyrosequencing. Sequence analysis showed that the drug resistance sites of HBV P region located at sites 173; 180; 181; 184; 204; 236 and 250. The main site of HBV P region drug resistance was 204 and 180, accounting for 35.8% and 23.5%, respectively. There were significant differences in the mutation rate of site 180 among different age groups. There were also significant differences in the mutation rate of site 204 among younger than 30 age group, 41 to 50 age group and 51 to 60 age group. (P < 0.05, P < 0.01). The mutation rate of site 180 combined with site 204 was 66.6%. The mutation rate of site 181 combined with site 236 was 23.3%. The age of C genotype infected patients was significantly older than B genotype infected patients (P < 0.01). M204V/I mutation mostly existed in the form of joint L180M mutation, the mutation rate was age-related. The detection of HBV genotypes and drug resistance sites of HBV P region have important clinical implications for the treatment and prognosis of patients with CHB.
Adult ; Aged ; Antiviral Agents ; pharmacology ; China ; Drug Resistance, Viral ; Female ; Gene Products, pol ; genetics ; Genotype ; Hepatitis B virus ; classification ; drug effects ; enzymology ; genetics ; Hepatitis B, Chronic ; drug therapy ; virology ; Humans ; Male ; Middle Aged ; Mutation, Missense ; Young Adult

OBJECTIVE:To study the effects of mifepristone on related indexes of patients with myomectomy. METHODS:The information of 100 patients with uterine fibroids were analyzed retrospectively,and they were divided into observation group and control group according to therapy method,with 50 cases in each group. Control group only received myomectomy. Observa-tion group was given Mifepristone tablets 10 mg orally,qd,for consecutive 3 months,and then received myomectomy within 2 d after drug withdrawal;they were given Mifepristone tablets 10 mg/d orally after surgery,for consecutive 3 months. The 2 groups were followed up for 6 months. The maximual volume of uterine fibroids and the volume of uterus were compared between 2 groups as well as related operation indicator,levels of serun reproductive hormones,sex hormones of uterine myoma tissure and re-lated growth factors of uterus myomas. The recurrence and occurrence of ADR was recorded during treatment. RESULTS:Before surgery,the maximual volume of uterine fibroids and the volume of uterus in observation group were significantly lower than control group,with statistical significance(P<0.05). The operation time,vaginal bleeding time and hospital stay of observation group were significantly shorter than those of control group,the intraoperative blood loss and the recurrence rate were significantly lower than those of control group,with statistical significance (P<0.05). Six months after surgery,ER,PR,EGFR,TGF-β1R and IGF-1R of uterus myomas in observation group were significantly lower than those of control group,with statistical significance (P<0.05). There was no statistical significance in the levels of FSH、E2 and P level of uterus myomas between 2 groups in first di-agnosis and 6 months after surgery(P>0.05). No obvious ADR was found in 2 groups during treatment. CONCLUSIONS:Mife-pristone helps to reduce operative time,intraoperative blood loss,vaginal bleeding time and hospital stay of patients with myomecto-my,and may be related to the regulation of ER and PR levels and related growth factor receptors.

Objective:To evaluate the current situation and the developing trend of 5-HT3 receptor antagonists used in 25 hospital of Wuhan during the period of 2003～2005 in order to provide reference for clinical doctors and pharmacists. Method:The 5-HT3 receptor antagonists were statistically analyzed in respect to the purchase sum of money,DDDs and daily costs. Result:During the 3 years, purchase sum of money of 5-HT3 receptor antagonists were yearly increasing. The share of the market of domestic drugs showed an increasing tendency,while the imported drugs decreased year by year. Granisetron, ondansetron and tropisetron were much more frequently used than others. Conclusion:The market of the oral agents of 5-HT3 receptor antagonists will further expand in China.

Many studies had shown that the stigma is the important factor for diagnosis,treatment and re-covery of bipolar disorder.The stigma not only has negative effects on the patients and also on the marriage,fami-lies,study,work,medication treatment,and many other aspects.This paper reviews the development of definition of stigma,the characteristics,effects,interventions of stigma of bipolar disorder,finally,make a summary and put for-ward the prospects.

BACKGROUND:We tried to combine 8-bromo-7-methoxychrysin and sorafenib in order to offset the tolerance of hepatocelular cancer stem cels to sorafenib, thereby comprehensively improving the therapeutic efficacy on hepatocelular carcinoma. OBJECTIVE:To observe the effects of 8-bromo-7-methoxychrysin, sorafenib and their combination on apoptosis of liver cancer stem-like cels SMMC-7721, and to analyze their mechanisms. METHODS:SMMC-7721 cels were treated with 8-bromo-7-methoxychrysin, sorafenib alone and their combination for 24 hours. Then, flow cytometry was used to detect cel apoptosis and western blot assay was used to determine nuclear factor-κB protein expression. RESULTS AND CONCLUSION:Compared with 8-bromo-7-methoxychrysin group and sorafenib group, the apoptotic rates of SMMC-7721 cels were significantly enhanced after treatment with the combination of 8-bromo-7-methoxychrysin group and sorafenib (2.5, 5, 10, 50 μmol/L), and meanwhile, the protein expression of nuclear factor-κB was down-regulated significantly. These findings indicate that the combined therapy of 8-bromo-7-methoxychrysin group and sorafenib can enhance the apoptosis of SMMC-7721 cels, which may be associated with down-regulation of nuclear factor-κB protein.

Objective To investigate the clinical features of children with systemic lupus erythematosus (SLE).Methods Ninety-five patients hospitalized in Wuxi Children's Hospital from Jan.2003 to Dec.2013 were analyzed retrospectively.They were diagnosed as SLE for the first time.The clinical features of SLE between boys and girls were compared.Chi-square test was used for statistical analysis.Results Of the 95 cases with SLE,12 were boys and 83 were girls.Clinical manifestations showed 69 cases with facial rash (72.6％),43 cases with fever (45.3％),31 cases with arthritis (32.6％),24 cases with serositis (25.3％),23 cases with oralulcer (24.2％),13 cases with allergy to sunlight (13.7％),11 cases with nervous system damage (11.6％).Laboratory examination:94 cases (98.9％) with anti-nuclear antibody positive,91 cases (95.6％) with decreased C3 complement,71 cases (74.7 ％) with anti-dsDNA antibody positive,71 cases(74.7％) with decreased C4 complement,69 cases(72.6％) with anti-SSA antibody positive,62 cases (65.3％) with anti-Sm antibody positive,52 cases (54.7％) wiht proteinuria,anti-SSB antibody positive 49 cases(51.6％),46 cases (48.4％) with decreased leukocyte,38 cases(40.0％) with anti-nucleosome antibody,38 cases (40.0％) with anemia,26 cases(27.4％) with hematuresis,14 cases(14.7％) with decreased platelet,6 cases(6.3％) with injury of renal function.Renal injury,anti-Sm antibody and anti-nucleosome antibody of male children with SLE were significantly higher than those of female children(x2 =9.989,4.224,4.070,all P ＜ 0.05).The positive rate of anti-SSB antibody in female children with SLE was significantly higher than that of male children(x2 =3.885,P ＜ 0.05).Conclusions Male childre with SLE are easy to appear the renal injury,proteinuria is the most common.Anti-Sm antibody is a risk factor for the high incidence of renal injury in male children with SLE.Anti-nucleosome antibody is a risk factor for the high incidence of SLE disease activity in male children than that of female children.The risk of future Sjogren's syndrome in female children with SLE is higher than that of male children.

Objective To investigate the effect of transverse incision surgery for epicanthoplasty with double eyelid fold reconstruction. Design Retrospective case series. Participants 103 patients with epicanthus. Method Transverse incision was applied for epicanthoplasty in all patients, and incorporated with double eyelid fold reconstruction. Patients were followed up 3-6 months. Main Outcome Measures Size of lacrimal caruncle, length of palpebral fissure, inner canthic diameter and scarring degree. Results Lacrimal caruncle was revealed 3/4 merous in 21 cases, 2/3 merous in 56 cases, 1/2 merous in 8 cases. Extent of palpebral fissure was 30-31mm in 5 cases, 28-29 mm in 69 cases, 26-27 mm in 11 cases. Inner canthic diameter was 32-33mm in 61 cases, 30-31 mm in 23 cases, 29 mm in 1 case. The scarring was little in 56 cases, obviously in 29 cases. Epicanthus relapsed in 2 cases. 16 cases lost follow-up. Conclusion Transverse incision surgery for epicanthoplasty with double eyelid fold reconstruction has less tissue damage and minimal scarring produced at the medial cantha. It is a simple and reliable surgical approach for correction of epicanthus.

China is one of the countries where the prevalence rate of endemic fluorosis is very high.There are about one hundred million people suffering fluorosis.Many researchers are paying more and more attention to this field.Recently,some researches indicated that particular nutrients can influence fluorosis.This article reviewed the relationship between intake of protein and energy,mineral,vitamin and the occurrence and sequelae of fluorosis.