OBJECTIVE:To evaluate the effect of temozolomide(TMZ)in combination with dexamethasone(DXM)on the proliferation of human gliocytoma U251 cells in vitro.METHODS:Human U251 cells were assigned to 1 of the 3 groups: TMZ(10,25,50,100,200,400?mol?L~(-1),respectively)alone or in combination with 40?mol?L~(-1)DXM(TMZ+DXM group)or control group(none drug).After treatment for 72 hours,the cell morphology,cell inhibition ratio,cell cycle and the apoptotic rate were detected.RESULTS:When TMZ concentration was greater than 100?mol?L~(-1)the cell inhibition ratio was higher in TMZ-treated group than in TMZ+DXM-treated group(P

The event of “diagnosing mental disorder but actually not” violates patients′right of informed con-sent and refused to health , showing some problems of “diagnosing mental disorder but actually not” and mandatory admission process and psychiatric judicial authentication , and also have some ethical problems .Therefore, relevant departments should perfect the laws and regulation , establish a hospitalized psychiatric patients appeal , the appeal channels , at the same time , physicians should follow a correct understanding , respect patients rights , disinterest-ed, the ethical principles of self -supervision and also use constraints should be regulated .

Objective To study the effect and the mechanism of Tongxieyaofang (TXYF) on rat model of irritable bowel syndrome (IBS). Methods Model rats of IBS which were prepared by chronic stimulation in colon were randomly divided into groups: the normal control group (Group A), the model control group (Group B), the positive control group (Group C), the low dosage of TXYF group (Group D), the high dosage of TXYF group (Group E). Different treatments were given to every groups by ig administration for one month. The capability limens of the sacculus that caused abdomen-uplifting and back-arching and the times of contract of abdomen muscle in rats under different dilatations were observed. The levels of 5-hydroxytryptamine (5-HT), substance P (SP), and calcitonin gene related peptide (CGRP) were evaluated. Results Compared with the Group B, the TXYF could effectively improve the index of the behavior and electrophysiology, it also could lower the levels of 5-HT and SP, and increase the levels of CGRP of rat model of IBS with certain dose-effect relationship. Conclusion TXYF could lower the levels of 5-HT in serum and SP in plasma, and increase CGRP level of model rats of IBS, especially in high dosage. The mechanism of TXYF may be increasing the pain threshold of bowel, eliminating hypersusceptibility of bowel, and decreasing excitability of nerve cells by decreasing the 5-HT and SP levels in IBS rats.

Objective:To evaluate the current situation and the developing trend of 5-HT3 receptor antagonists used in 25 hospital of Wuhan during the period of 2003～2005 in order to provide reference for clinical doctors and pharmacists. Method:The 5-HT3 receptor antagonists were statistically analyzed in respect to the purchase sum of money,DDDs and daily costs. Result:During the 3 years, purchase sum of money of 5-HT3 receptor antagonists were yearly increasing. The share of the market of domestic drugs showed an increasing tendency,while the imported drugs decreased year by year. Granisetron, ondansetron and tropisetron were much more frequently used than others. Conclusion:The market of the oral agents of 5-HT3 receptor antagonists will further expand in China.

Objective To observe the clinical efficacy of S-1 plus oxaliplatin (SOX) in neoadjuvant chemotherapy for patients with local advanced gastric carcinoma.Methods Clinicopathological data of 68 patients with local advanced gastric cancer were retrospectively analyzed and enrolled as treatment group.All the patients in the treatment group achieved 3-4 cycles chemotherapy of SOX regimen preoperatively.Outcomes of tumor remission after neoadjuvant chemotherapy were evaluated according to RECISI1.1 criteria.Meanwhile,70 patients who underwent operation directly after pathological diagnosis of local advanced gastric cancer during the same period were recruited as control group.Resection rate,R0 resection rate,postoperative complications rate and postoperative progression free survival(PPFS) time were compared between the two groups.Results In the treatment group,after neoadjuvant chemotherapy,4 cases (5.9％) achieved complete renission,36 cases (52.9％) achieved partial remission.Meanwhile,the numbers of cases with stable disease and progressive disease were 15 cases(22.1％) and 13 cases (19.1％) respectively.The objective remission rate and disease control rate in the treatment group were 58.8％ and 80.9％ respectively.The tumor resection rate,R0 resection rate,postoperative complications rate in the treatment group were 92.6％,85.3％ and 14.7％ respectively.The R0 resection rate in the treatment group was higher than that in the control group,the difference was statistically significant(x2 =8.042,P ＜ 0.05).However,the differences of tumor resection rate and postoperative complications rate between the two groups were not statistically significant (x2 =1.711,0.673,all P ＞ 0.05).The median time of PPFS in the treatment group and the control group was 36 months and 30 months respectively,there was statistically significant difference in PPFS between the two groups (x2 =4.049,P ＜ 0.05).Conclusion The neoadjuvant chemotherapy of S-1 plus oxaliplatin for local advanced gastric carcminoma has satisfactory short-term effect and can also improve PPFS.However,further investigation is required to observe the effect of this neoadjuvant chemotherapy regimen on postoperative overall survival.

BACKGROUND: Distal humerus fracture is often comminuted, and is difficult to receive reduction and fixation due to its special anatomical structure. Choosing which surgical approach is still a controversy.OBJECTIVE: To compare and analyze the curative efficacy of the perpendicular double-locking plating system for the internal fixation of type C distal humerus fractures through two kinds of postcubital approaches.METHODS: Thirty-two cases of type C distal humerus fractures were enrolled and divided into groups A (osteotomy of olecranon approach) and B (tricep anconeus flap approach). There was no significant difference in the baseline data between two groups. The operation time, intraoperative blood loss, hospital stay, healing time,postoperative follow-up and complications were recorded and analyzed. The patients were followed up at 2 weeks, 1, 3,6, 12, and 18 months. The healing time and complications were recorded according to radiographs and physical examinations. The Mayo Elbow Performance Score was used to determine the elbow function at 1 year postoperatively.RESULTS AND CONCLUSION: (1) The group A had a longer operation time and more intraoperative blood loss than those in the group B (P < 0.05), but showed a better elbow flexion and extension degrees, and higher Mayo Elbow Performance Score at 1 year postoperatively (P < 0.05). (2) There was no significant difference in the healing time between two groups (P > 0.05). (3) These results suggest that the articular surface of the distal humerus fracture is displayed well through two approaches, and the perpendicular double-locking plating system for the internal fixation of type C distal humerus fractures is rational. Noticeably, the tricep anconeus flap approach dose little damage to the muscle, while the osteotomy of olecranon approach obtains better elbow function and less complications.

Chronic intestinal pseudo-obstruction(CIPO)is an intestinal motility dysfunction caused by intestinal myopathies or neuropathies or mesenchymopathies.The clinical manifestation is almost the same with mechanical intestinal obstruction,but in the absence of any obstruction in the gastrointestinal tract.According to etiology,it can be divided into primary and secondary.While according to gastrointestinal neuromuscular disorders,it can be categorized as myopathy,neuropathy and mesenchymopathy,which have different pathogenesis.The disease with the clinical manifestation,imaging examination or histopathology was diagnosed,but mechanical intestinal obstruction must be excluded.Presently,the therapies of CIPO mainly are stimulating the motility of intestine and ensuring the necessary nutrition of the children.The review is mainly focused on the research progress of the pathogenesis,clinical manifestation,diagnosis,therapy of pediatric CIPO in recent years.

0.05. CONCLUSION:Kaixin Powder could enhance VD rats' memory; while Tacrine could enhance VD rats' learning; Kaixin Powder could enhance VD rats' learning and memory as well as Tacrine. Tacrine could down-regulate AChE mRNA expression in hippocampus of VD rats,further studies are needed to elucidate the effects of Kaixin Powder on memory performance in VD rats.

Objective:to combine psychotherapy and medication in treatment of tension-type headache Method:84 patients with tension-type headache were randomly divided into study group (combining psychotherapy with medication) and control group (medication only) The psychotherapy was twice to three times a week, for 2 or 3 weeks and then follow up for at least 1 year Result:in the study group, the rate of improvement was 95%, that of marked improvement was 67%, were significantly higher than the counterparts in control group (were 69% and 38% respectively) Conclusion:combining psychotherapy with medication is more effective than medication only in treatment of tension-type headache