Objective: The purpose of this study was to investigate the effect of TNF-? on osteoclast differentiation in primary murine bone marrow cell culture with and without RANKL. Methods: M-CSF-dependent bone marrow cells were isolated from 5-6 weeks old mice, and cultured in the presence of M-CSF (25 ?g/L) with different concentrations of TNF-? (0, 1, 10, 100 ?g/L) for 5 days, the formation of TRAP(+) multinucleated cells was observed. These cells were also cultured in the presence of both RANKL (30 ?g/L) and M-CSF (25 ?g/L) with or without 10 ?g/L TNF-? for 4, 5, 6 and 9 days. The number of TRAP(+) multinucleated cells and resorption pits on dentine slices were counted under light microscope. Results: In the absence of RANKL, TNF-? was unable to induce osteoclast formation from murine bone marrow precursors. In the presence of RANKL, TNF-? augmented osteoclastogenesis and bone resorption, and this effect occurred only on the early stage. Conclusion: TNF-? enhances RANKL-induced osteoclast formation and function, but can’t substitute for RANKL. TNF-? stimulates osteoclast differentiation, but not survival.

Objective To evaluate the efficacy and safety of small dose Urapidil in the treatment of patients with congestive heart failure. Methods 60 patients with congestive heart failure (NYHA class Ⅲ～Ⅳ) were randomly assigned to treatment with Urapidil (n=30) and Nitroglycerin (n=30). Intravenous Urapidil (100?g/min) or Nitroglycerin (10～20?g/min) was administered for 5～7 days in addition to ordinary treatment. Heart rate, blood pressure were measured and echocardiography was performed before and after the treatment. And side effects were recorded. Results The total effective rate was 86 7% in the Urapidil group, and 90% in Nitroglycerin group. No significant differences of heart rate and blood pressure were found after treatment between two groups. The ejection fraction measured by echocardiography increased significantly in two groups after the treatment, but more significantly in the Urapidil group. Conclusions It is efficacious, safe and well tolerated for intravaneus small dose Urapidil to treat congestive heart failure.

Objective To explore the allergic status and allergen composition in children with acute Henoch-Schonlein Purpura in Wuxi by allergen detection, thereby to establish appropriate therapeutic strategies. Methods Two hundred and forty-six patients with Henoch-Schonlein Purpura in their acute phase were examined for serum concentrations of total IgE and specific IgE using Germany AllergyScreen system. Results The positive rate of allergen-specific serum IgE was 48.7% (120/246) ,of which,positive reactions to two or more than two allergens were found in 86 cases (34.9%) and that to one kind of allergen were found in 34 cases (13.8%)The positive rate of total serum IgE was 78.9% (194/246). In inhalation allergen group, positive reaction to allergen was found in 134 cases (54.5%). The most common allergen was dust mite/flour mites (19.1%) ,and came the next were house dust(17.1%) and Penicillium notatum/Alternaria alternata(10.2%). In food allergen group,positive reaction to allergen was found in 77 cases (31.3%). The most common allergen was fish (6.5%),and came the next were egg (5.7%) and mutton (5.3%). We found no significantly different allergen positive rates between inhalation allergens group and food allergens group (χ2 = 2.59, P ＞ 0.05). Conclusions The combined allergens attacks were more common in children patients with acute Henoch-Schonlein Purpura by serum allergen detection. Various types of the allergens are associated with the occurrence of acute Henoch-Schonlein Purpura. To reduce the harmness of allergens,it is necessary to avoid touching these allergens.

Objective To investigate the impact of motor imaginary therapy on the recovery of upper limb function in acute cerebral infarction patients with bemiplegia. Methods Fifty cerebral infarction patients with hemiplegia were randomly divided into a control group (drug therapy + early exercise therapy) and a motor imaginary therapy group (drug therapy + early exercise therapy + motor imaginary therapy) using single-blind method (n = 25 in each group). Ipsilateral upper extremity function was assessed by the Fugl-Meyer assessment (FMA) before the treatment and at day 40. The active range of motion (AROM) of the ipsilateral wrist was measured by a conimeter. Eating, washing, dressing, and putting on and off clothes were assessed by the Functional Independence Measure (FIM) scale. Results The scores of FMA, AROM and FIM were increased more significantly than those before the treatment in both groups (P <0.05). All the scores in the motor imaginary therapy group after the treatment were superior to those in the control group (P <0. 05) (FMA 28.33 ± 8.63 versus 15.93 ± 5.39;AROM 19.55 ± 8.30 versus11.97 ± 6.59; FIM 16.83 ± 5.43 versus 12.51 ± 3.36).Conclusions Conventional motor rehabilitation training + motor imaginary therapy during the acute phase may promote the motor function reco,,ery in cerebral infarction patients with hemiplegia.

Abstracting and Indexing as Topic ; Humans ; Pediatrics ; Periodicals as Topic ; Translations ; Writing

The perineurial cells of the small nerve branch of the normal human abdominal wall are observed with electron microscope. The fibrous long-spacing bodies (FLS) are found within the interstitial substance of the endoneurium. 1. 2-5 layers of the perineurial cells surround the nerve fasciculus. The perineurial cells are squamous in shape and the cytoplasm contains microfilaments and pinocytotic vesicles. Each perineurial cell has an obvious basement membrane on its basal surface but the fibroblasts, whatever situated in the endoneurium or on the outer surface of the perineurium, has no basement membrane. Numerous desmosome and some gap junctions between the close attached perineurial cells are demostrated. The collagen fibrils between perineurial cells can be often shown. FLS bodies and collagen fibrils about 45 nm in diameter in the interstitial substance of endoneurium inside perineurial cells are demonstrated. In the connective tissue surrounding the outside of perineurial cells, the collagen fibrils of 80 nm in diameter can be seen, but no FLS bodies present there. 2. FLS bodies in the endoneurial matrix can be demostrated. Most of them closely associated with the basement membrane of the Schwann cells surrounding the unmyelinated nerves but no FLS bodies are found associate with those of the myelinated nerves. A few FLS bodies do not relate to basement membrane and they are invested only with the collagen fibrils. In the longitudinal sections, most of FLS bodies are in spindle shape of various sizes, their longitudinal axes parallel to that of the adjoining collagen fibrils. Sometimes the FLS bodies continue with the collagen fibrils are found. Occasionally, FLS body like a bridge locate between two Schwann cells and its dark bands continue to the basement membrane of the Schwann cells. Two or three FLS bodies may fuse together but their cross bands do not registered at the same level. In the oblique sections, FLS bodies are nearly rectangular in shape, with the cross bands shown, and their appearance do not show greaty difference from those in longitudinal sections. The periodicity of FLS bodies is about 133nm in length; the dark band in it is about 53 nm, which is made of the dense granular substance; the light band is about 80 nm, which is made of a network with approximated parallel microfilaments. There are no further crossstriated structures within the periods. Some problems, such as the role of the perineurial cells, the relationship of FLS bodies with basement membrane, are briefly discussed.

The hallmark of osteoarthnitis(OA) is the loss of articular cartilage caused by an imbalance between cartilage synthesis and degredation over a variable period of time.IL-1 plays an important role in the course.The IL-1receptor antagonist(IL-Ra) specific against IL-1 is becoming a pivot of wide attention.The article offers a review of the relationship between the IL-1Ra and OA.

Objective To study the effect of Shugan Tongluo Recipe on the patients with upper limb swelling after breast cancer surgery, and to explore the efficiency of treatment.Methods84 patients with breast cancer were selected from June to December 2015 in the traditional Chinese medicine clinic of Ge zhen,, were randomly divided into control group and observation group, control group using beta seven yezao sodium and conventional rehabilitation therapy, observation group based on the combined application of Shugan Tongluo Decoction, and compared the effectiveness of treatment.ResultsThe effective rate of the observation group was 92.8%, significantly better than the control group 78.6%, the difference between the two groups significantly, with statistical significance (P<0.05).At the same time, the observation group VAS score, IL-6 score and CCL-18 score were lower than the control group, EORTC QLQ score is higher than that of the control group, the treatment of feedback better(P<0.05).In addition, the incidence of adverse reactions was 4.7%, 9.5% in the control group, the observation group treatment.ConclusionThe prescription of Shugan Tongluo decoction has a good effect on the treatment of upper limb swelling after breast cancer surgery, and it has high safety and good patient satisfaction.

Objective:To screen the issues of management for radiation therapy equipment in an academic hospital with Delphi method.Methods: 6 specialists with different background were consulted with the questionnaire, and those questions were screened from questionnaire as being scored with the importance and analyzed by SPSS software.Results: 6 specialists included 2 radiation managers, 3 radiation professionals and 1 health administrative personnel. The authority coefficient was 0.88±0.06, and the activity coefficient was 100%. The Kendall concordance coefficient wereW=0.019(P=0.635) andW=0.237(P=0.001) in the 1st and 2nd round respectively. The coordination of the expert’s opinions was good and the results of the questionnaire were credible. There were 18 questions got from the questionnaire based on the averages and standard deviations.Conclusion: It was effectiveness that Delphi method can screen the issues of management for radiation equipment, on which we can get the direction of performance improvement based.

Objective To investigate the effects of early core muscles and manual respiratory function training on stroke patients with dysphagia. Methods From June, 2015 to January, 2016, 60 stroke patients with dysphagia were divided equally into control group and obser-vation group randomly. Both groups accepted routine swallowing function training, electrical stimulation and respiratory function training, while the observation group accepted core muscles training and manual respiratory function training, for four weeks. They were evaluated with Standardized Swallowing Assessment (SSA), forced vital capacity (FVC), maximum ventilatory volume (MVV) and the maximum ex-piratory time before and after treatment. Results All the indices improved in both groups after treatment (P<0.001), and improved more in the observation group than in the control group (P<0.001). Conclusion Core muscles and manual respiratory function training at early stage can obviously improve swallowing and respiratory function of stroke patients with dysphagia.