Objective:To investigate the changes of the protein expression in heart tissue of rats after ischemia-reperfusion(I/R) injury and pre-treatment of carvedilol, and to elucidate the new protective mechanism of carvedilol pre-treatment to I/R heart injury. Methods:18 rats were randomly divided into sham-operation group, I/R injury group and carvedilol pre-treated group. The model of myocardial I/R injury was established by 30min ligation of the left anterior descending branch of the coronary artery and 2h reperfusion. Serum malondiadehyde (MDA) was determined, and the cytosolic fraction of myocardial tissue homogenate was separated applying two-dimensional gel electrophoresis technique. Differentially expressed protein spots were figured out with PDQuest software, while preliminary protein-identification was carried out by means of the two-dimensional gel electrophoresis database on the web. Results:Compared with the sham-operation group, after I/R injury, serum MDA was increased(P

OBJECTIVE: To investigate the clinical application of carbon nanoparticles staining in cervical lymph node dissection on clinical neck lymph nodes the negative (cN0 period) thyroid papillary thyroid carcinoma (PTC). METHOD: This retrospective analysis comprised 100 papillary thyroid cancer patients who met inclusion criteria,and they were randomly divided into the nano-carbon group (50 cases) and control group (50 cases). They underwent lobectomy, subtotal thyroidectomy or total thyroidectomies and were given elective central compartment neck dissection (CCND). The number of detected lymph nodes in each group was summed, and pathological examination was conducted. The number of lymph nodes (dyedor not dyed) and the location of metastatic nodes were recorded separately. RESULT: In the nano-carbon group the average number of eliminated lymph nodes is significantly more than that of the control group(P<0.05). The metastasis lymph nodes in the nano-carbon group was higher than that in the control group(P<0.01). Among 100 cases, 2% had temporary vocal cord palsy, and 8% had temporary hypoparat hyroidism. No case of permanent vocal cord palsy or permanent hypocalcemia was observed. CONCLUSION The dyed lymph nodes can be easily identified and can be used as a guide for lymphnodes dissection in papillary thyroid carcinoma operation.
Carbon ; Carcinoma ; pathology ; surgery ; Carcinoma, Papillary ; Coloring Agents ; Humans ; Lymph Node Excision ; Lymph Nodes ; Lymphatic Metastasis ; diagnosis ; Nanoparticles ; Neck ; Neck Dissection ; Radiopharmaceuticals ; Retrospective Studies ; Thyroid Cancer, Papillary ; Thyroid Neoplasms ; pathology ; surgery ; Thyroidectomy ; Vocal Cord Paralysis

Objective To pave the way for clinical therapy and reasonable administration of antibiotics, and to analyze the kinds and drug-resistance of pathogens of nosocomial infection in patients with spinal cord injury. Methods Bacteriology of infection and drug resistance were analyzed in 395 patients with spinal cord injury in the hospital from Jun., 2012 to Dec., 2013. Results There were 132 cases with nosocomial infection. Most of the infections were found in urinary tract (71.1%) and lower respiratory tract (24.2%). The main pathogenic germs were Escherichia coli (45.6%), P. Aeruginosa (18.1%), K. pneumonia (11.4%) and P. mirabilis(8.7%), which were resistant to the second or third generation cephalosporins and quinolones moderately or severely, but sensitive to β-lactamase inhibitor combinations and aminoglycosides. Conclusion The main pathogenic bacteria in nosocomial infection of patients with spinal cord injury are G- bacilli. The bacteria are resistant moderately or severely to antibiotics, which should be selected rationally by bacterial culture and drug susceptibility test, and optimized rationally with PK/PD (pharmacokinetics and pharmacodynamics).

OBJECTIVEThis study aimed to evaluate the effect of resin infiltration and fluoride solution on masking different demineralized white spot lesions by assessing color change.

METHODSArtificial white spot lesions were produced on 60 human molars. Each sample had at least two enamel surfaces opened (named A and B). The samples were randomly divided into groups 1, 2, and 3 according to their time of demineralization (24, 48, and 72 h). After demineralization, the A spot of each sample was treated by resin infiltration. The B spot was treated with 0.1% fluoride solution daily for 30 days. After the remineralization of the B spot, resin infiltration was used again on the B spot of each sample. Color assessment was performed by a spectrophotometer in five distinct stages: baseline, after the production of artificial caries, after resin infiltration of A spots, after 30 days of fluoride solution treatment of B spots, and after resin infiltration of remineralized B spots.

RESULTSBefore demineralization, the L* values of spots A and B in all groups were not significantly different (P > 0.05), whereas the L* values of spots A and B were significantly increased after demineralization. The L* values of A spots recovered significantly after treatment by resin infiltration (P<0.05), but only groups 1 and 2 reached the baseline. The L* values of B spots had no significant differences (P > 0.05) after fluoride treatment compared with that after demineralization. After resin infiltration on B spots, the L* values recovered but could not reach the baseline nor the level of A spots treated by resin infiltration only.

CONCLUSIONResin infiltration is a more effective treatment for masking white spot lesions than traditional fluoride treatment. The effect of masking white spot lesions has certain relationships with the degree of demineralization and activity of the lesion.

Objective To investigate the status quo and characteristics of medical students'job choosing view,to analyze the reasons and to propose countermeasures.Methods Questionnaires on job choosing view concerning job choosing cognition,ideals,method,motivation and values were conducted among 1700 medical students in Shandong University.Data entry and statistics were done by SPSS 17.0.Difference was analyzed by t-test and ANOVA.Results Significant differences were showed in sex,whether being the only-child in the family,father's education and family's economic status(P＜0.05).Conclusions Medical students'job choosing views are characterized by professional,highly expected,diversified and utilitarian,which is caused by society,school and family.Therefore,much importance should be attached to improving educational environment,making educational objective clear and constructing educational content system.

Objective To discuss incidence and related risk factors of vascular cognitive impairment (VCI) after acute subcortical infarction (SI).Methods One hundred and eighty-two patients with acute SI were divided into two groups:VCI group (81 cases) and no-VCI group ( 101 cases) according to VCI diagnostic standard.Sex proportion,age,culture degree between two groups were compared,and correlation was analyzed between the quantity of risk factors and the incidence of VCI.Results The incidence of VCI after acute SI was 44.5％(81/182).According to age stratification,the incidence of VCI in the patients of 56-65 years was 35.4％ (23/65),in the patients of 66-75 years was 45.3％ (48/106),and in the patients of 76-85 years was 90.9％ (10/11 ).The age in VCI group was significantly higher than that in no-VCI group [(69.93 ±6.91) years vs. (67.62 ±5.56) years,P =0.014],and culture degree was significantly lower than that in no-VCI group (P =0.028).The sex proportion between two groups had no significant difference (P =0.876).The more accompanying risk factors,the higher incidence of VCI.Conclusions Along with the increase of age,the incidence of VCI after acute SI is higher.The more accompanying risk factors,the higher incidence of VCI.

Objective: To explore rational drug usage for periodontitis treatment through a comparative study of the efficacy of four kinds of drugs on the experimental periodontitis. Methods: 126 Wistar rats,10 randomly selected as normal control group, the other 116 were modeled by using local wire ligation and systemic prednisolone acetate injection. Successful animal models were randomly divided into model control group and berberine hydrochloride, minocycline hydrochloride, ginsenoside Rg-1 and transforming growth factor-β1 treatment groups, which were treated with each kind of medicine separately and were killed at the end of 1, 2, 4 weekend. Detected IL-1β, TNF-α, IL-6, BGP levels by immunohistochemical SABC assay. Results: IL-1β, TNF-α, IL-6 of the treatment groups were lower and BGP was higher than model control group(P<0.05).Among them, the role of berberine hydrochloride to reduce IL-1β and TNF-α was the best. The role of minocycline hydrochloride to reduce IL-6 was the fastest. The role of transforming growth factor-β1 to increase BGP was the fastest. The role of ginsenoside Rg-1 to increase BGP was more lasting and better follow-up. Berberine and ginsenoside Rg-1 reduced IL-1β, TNF-α, IL-6 and increased BGP at the same time. Conclusion: The treatment of minocycline hydrochloride and transforming growth factor-β1 are quick. Berberine and ginsenoside Rg-1, particularly berberine have the advantage of multi-targets' role.

Heat shock protein (HSP) 105 is a 105x103 stress protein that belongs to the HSP-110 family. It is released by tissues in response to a wide variety of stresses including infection and ischaemia. Studies have shown that the molecule is involved as a biochemical mediator of heat induced apoptosis by binding to p53 at scrotal temperatures and dissociating from it at suprascrotal temperatures in testicular germ cells. Recent studies have shown that HSP-105 is over-expressed in various malignancies besides in normal testicular tissue. HSP-105 may be a candidate of testis antigen.

Objective To study the expression of both nuclear factor-κB(NF-κB)and tumor necrosis fac-tor-α(TNF-α)in pulmonary intravascular macrophages(PIM)of rats with hepatopulmonary syndrome (HPS)and the effect of pyrrolidine dithiocarbamate(PDTC)on their expression.Methods The Sprague-Dawley(SD)rats were randomly divided into four groups:control,control+PDTC1CCl4,CCl4+PDTC groups.Arterial blood was collected for measurement of blood gas.Venous blood was sampled for hepatic function and endotoxin level.The mesenteric lymph nodes were dissected for bacteriology studies.Proteins of NF-κB and TNF-a of lung tissue were examined by immunohistochemistry.The activity of NF-κB in lung tissues was measured using electrophoretic mobility shift assay (EMSA).By real-time polymerase chain reaction (PCB)using SYBR Green I,the mRNA expression of TNF-α in lung tissues were detected.Results CCl4 group developed HPS with decreased PaO2 and PaCO2,increased alveolar-arterial oxygen difference(A-aDO2),abnormal hepatic function and increased endotoxin level.Culture-positive mes-enteric lymph nodes were found in 62.5%(5/8)of CCl4,group and 66.7%(6/9)of CCl4+PDTC group(P>0.05 J.All lungs from CCl4 and CCl4+PDTC group showed no accumulation of larse mononuclear macrophagelike cells within the lumen of numerous small muscular and nonnulscular pulmonary vessels.The percentages of vessels with more than 1O adherent macrophages was 60.8%(292/480)in CCl4 group but only 19.6%(106/540)in CCl4+PDTC group(P<0.01).The protein expression of NF-κB and TNF-α Was localized to PIM in CCl4 group.The NF-κB activity and mRNA expression of TNF-α in CCl4 group was significantly higher than that in control,con-trol+PDTC group and CCl4+PDTC group(P<0.05).Conclusion The TNF-α expression in PIM induced by NF-κB play an important role in HPS.The inhibitor of NF-κB PDTC can repress PIM activation and decrease the ex-pression of TNF-α.As result.HPS severity is reduced.

Objective: To explore the Influence of ethics education on ethical beliefs of dual relationship of trainees who attend training of psychological consultation.Methods: Use of questionnaire,written and directed by "psychological counseling and treatment of ethical issues questionnaire(trainee attending psychological consultation training)" as a tool to evaluate,within the framework of Guangdong 368 trainees to investigate.Results: Compared with two groups of trainees,in refer to the ethical beliefs of dual relationship,the latter group who has a higher option of "not sure" shows varying degrees of decline in most of the items in the questionnaire.Two teams of trainees reach an identity of 80% or more(10 items in total) in the ethical judgment of dual relationship(choose "not").Two groups show significant differences in judging 5 items of the entries(p