Objective:To investigate the changes of the protein expression in heart tissue of rats after ischemia-reperfusion(I/R) injury and pre-treatment of carvedilol, and to elucidate the new protective mechanism of carvedilol pre-treatment to I/R heart injury. Methods:18 rats were randomly divided into sham-operation group, I/R injury group and carvedilol pre-treated group. The model of myocardial I/R injury was established by 30min ligation of the left anterior descending branch of the coronary artery and 2h reperfusion. Serum malondiadehyde (MDA) was determined, and the cytosolic fraction of myocardial tissue homogenate was separated applying two-dimensional gel electrophoresis technique. Differentially expressed protein spots were figured out with PDQuest software, while preliminary protein-identification was carried out by means of the two-dimensional gel electrophoresis database on the web. Results:Compared with the sham-operation group, after I/R injury, serum MDA was increased(P

Objective To pave the way for clinical therapy and reasonable administration of antibiotics, and to analyze the kinds and drug-resistance of pathogens of nosocomial infection in patients with spinal cord injury. Methods Bacteriology of infection and drug resistance were analyzed in 395 patients with spinal cord injury in the hospital from Jun., 2012 to Dec., 2013. Results There were 132 cases with nosocomial infection. Most of the infections were found in urinary tract (71.1%) and lower respiratory tract (24.2%). The main pathogenic germs were Escherichia coli (45.6%), P. Aeruginosa (18.1%), K. pneumonia (11.4%) and P. mirabilis(8.7%), which were resistant to the second or third generation cephalosporins and quinolones moderately or severely, but sensitive to β-lactamase inhibitor combinations and aminoglycosides. Conclusion The main pathogenic bacteria in nosocomial infection of patients with spinal cord injury are G- bacilli. The bacteria are resistant moderately or severely to antibiotics, which should be selected rationally by bacterial culture and drug susceptibility test, and optimized rationally with PK/PD (pharmacokinetics and pharmacodynamics).

OBJECTIVE: To investigate the clinical application of carbon nanoparticles staining in cervical lymph node dissection on clinical neck lymph nodes the negative (cN0 period) thyroid papillary thyroid carcinoma (PTC). METHOD: This retrospective analysis comprised 100 papillary thyroid cancer patients who met inclusion criteria,and they were randomly divided into the nano-carbon group (50 cases) and control group (50 cases). They underwent lobectomy, subtotal thyroidectomy or total thyroidectomies and were given elective central compartment neck dissection (CCND). The number of detected lymph nodes in each group was summed, and pathological examination was conducted. The number of lymph nodes (dyedor not dyed) and the location of metastatic nodes were recorded separately. RESULT: In the nano-carbon group the average number of eliminated lymph nodes is significantly more than that of the control group(P<0.05). The metastasis lymph nodes in the nano-carbon group was higher than that in the control group(P<0.01). Among 100 cases, 2% had temporary vocal cord palsy, and 8% had temporary hypoparat hyroidism. No case of permanent vocal cord palsy or permanent hypocalcemia was observed. CONCLUSION The dyed lymph nodes can be easily identified and can be used as a guide for lymphnodes dissection in papillary thyroid carcinoma operation.
Carbon ; Carcinoma ; pathology ; surgery ; Carcinoma, Papillary ; Coloring Agents ; Humans ; Lymph Node Excision ; Lymph Nodes ; Lymphatic Metastasis ; diagnosis ; Nanoparticles ; Neck ; Neck Dissection ; Radiopharmaceuticals ; Retrospective Studies ; Thyroid Cancer, Papillary ; Thyroid Neoplasms ; pathology ; surgery ; Thyroidectomy ; Vocal Cord Paralysis

The pattern of immunohistochemical localization of monoclonal antibody MC5 was observed in 215 cases of colarectal adenocarcinoma.It was found that the polarized pattern was predominant in the well-differentiated adenocarcinomas while the deffuse pattern in poorly-differentiated ade-nocarcinomas.Followup study of 133 cases revealed that 5-year sunvival rate was 74.4% in cases with polarized pattern and 36.2% in those with diffuse pattern,a very significant difference between the 2 (P

Nrf2/Keap1/ARE is an important signaling pathway in maintaining the balance between peroxide and antioxidants. Once oxidative stress happened, Nrf2/Keap1/ARE would be ac-tivated to regulate the expression of downstream antioxidant pro-teins that could reduce the injury from oxidative stress and the level of oxidative stress. Recent researches show that Nrf2/Keap1/ARE signaling pathway is closely related to refractory re-spiratory diseases such as pulmonary fibrosis (PF), lung canc-er, chronic obstructive pulmonary disease (COPD), suggesting that Nrf2/Keap1/ARE signaling pathway may be a potential ther-apeutic target. This review focuses on the role of Nrf2/Keap1/ARE signaling pathway in refractory respiratory diseases in order to further understand the related mechanism and provide evidence for the diagnosis and treatment of refractory respiratory diseases.

It is a challenge in diagnosis of hemophagocytic lymphohistocytosis (HLH).Rapid diagnosis is the key to make decisions in clinical management of HLH.The timely and reliable diagnosis of HLH can be assisted by laboratory tests including nature killer (NK) cell activity, sCD25, hemophagocytosis, ferritin, surface CD107a, and genetic testing.Here is introduced lab assays and evaluate the clinical applications in HLH.The aim is to raise awareness of the values of laboratory evaluations in diagnosing HLH.

Objective To discuss the application effect of TCM constitution recognition on TCM chronic disease refining health management for type 2 diabetes mellitus.Methods Totally 140 outpatients with type 2 diabetes mellitus from Chaoyangmen Community Health Center were selected and divided evenly into two groups after TCM constitution assessment. The study lasted for 2 years (1st August 2013–31st July 2015). The control group (70 cases) received diabetes community health management. In addition to routine community health management, TCM constitutional management was also administrated in the intervention group. The latter group received medicine, food, sport guidance according to the results of TCM constitution assessment. The blood glucose, blood lipid, BMI and waist-hip ratio of the two groups were monitored, and the self-management and life quality of patients were evaluated. Results After the intervention, significant improvement was found for fasting blood glucose, 2 h postprandial blood glucose, BMI, waist-hip ratio and blood lipid both in the control group and the intervention group, and the improvement in the intervention group was more obvious than the control group (P<0.05). The whole level of self-management and life quality was greatly increased in both groups, while improvement of the intervention group was much higher than the control group (P<0.05).Conclusion Integrated application of TCM constitutional theory in TCM chronic disease refining health management for type 2 diabetes mellitus will contribute to improving patients’ self-management, changing their clinical symptoms and finally improving their life quality.

Objective To investigate the flora of pathogens and antibiotic resistance of urinary tract infection after spinal cord injury. Methods From June, 2014 to June, 2015, 145 inpatients with urinary tract infection after spinal cord injury were reviewed. Results The main pathogenic germs were found as E. coli (48.9%), K. pneumonia (19.7%) and P. mirabilis (9.0%), and they were resistant to the second or third generation cephalosporins and quinolones moderately or severely, but sensitive toβ-lactamase inhibitor combinations and aminogly-cosides. The strategy of antibiotics need more reasonable. Conclusion The main pathogens in urinary tract infection after spinal cord injury are Gram-negative bacilli, that are resistant to antibiotics. The antibiotics should be selected and used rationally according to bacterial culture.

It is still contradictory whether PGI_2 and TXA_2 can influence hepatic is-chemic damage. Rats were divided into three groups at random. Group 1 and 2 were givensaline; group 3 were pretreated with indomethacin. 30 min before the induction of liverischemia drugs were injected into peritoneal cavity. After 2 h of ischemia and 2 hof reperfusion, the results showed that the TXB_2 content of ischemia group was higherand the P/T ratio was lower than that of the indomethacin group. The control groupshowed no change. Massive necrosis was observed in the ischemia group, but only focalnecrosis was seen in the indomethacin group. There was no hepatic necrosis in the controlgroup. The results suggested: 1. TXA_2 was an important cell injury agent; 2. Indomethacinhave protective effect on the reperfusion liver cell injury.

Objective To investigate the distribution and drug resistance of pathogen for pulmonary infection in cervical spinal cord inju-ry (CSCI) patients, and analyze the application of antibiotics. Methods From January, 2014 to June, 2015, 156 patients with CSCI were re-viewed. The bacteriology and drug resistance were investigated, as well as the application of antibiotics. Results There were 45 cases with nosocomial infection of 68 times, and 106 strains of bacteria in total. The top four Gram-negative pathogens were Klebsiella pneumonia (17.9%), Pseudomonas aeruginosae (15.1%), Encherichia coli (11.3%) and Acinetobacter baumannii (8.5%). The main Gram-positive cocci was Staphylococcus aureus (9.4%), and the main fungi was Candida tropicalis (0.7%). Gram-negative bacteria was resistant to the second or third generation cephalosporins and quinolones moderately or severely, but sensitive toβ-lactamase inhibitor combinations and aminoglyco-sides. The main Gram-positive cocci were sensitive to vancomycin. The application of antibiotics needed to be improved. Conclusion The Gram-negative bacteria are the chief pathogen for pulmonary infection in CSCI patients, which show multiple drug-resistant. It is significant to monitor the drug resistance of bacteria and optimize antibiotics.