ObjectiveTo investigate the outcomes of mother a nd baby smaller than 37 gestational age caused by preeclampsia. Method sThe data for 122 preterm birth that was single pregnancy with preecla mpsia in our hospital from January 1993 to December 2002 were collected. The pri nciple of diagnosis and treatment, the outcomes were analyzed retrospectively. T he 122 pregnant women were divided into 3 groups according to their gestational age, more than 28 weeks and smaller than 31 +6 weeks was the first group (28 cases), more than 32 weeks and smaller than 33 +6 weeks was the second grou p (26 cases), more than 34 weeks and smaller than 36 +6 weeks was the thir d group (68 cases). The data were statisted with SAS software.Results The earlier the pregnancy-induced hypertension happened, the earlier the preeclampsia occured, the mean time was 14 03 d, 16 85 d,22 22 d, respect ively, (P0.05), the outcomes of mothers were good. Increasing the gestational age under the cond ition that the occurrene of comptications was not increased, 60 64% pregnant wo men accepted the treatment of improving the maturity of fetal lung. There were n o differences among the three groups at the morbidity of perinatal SGA, intracra nial hemorrhage (P 0.05) .ConclusionWe could improve the outcomes of pret erm birth caused by preeclampsia by enhancing the perinatal care, treating and t erminating pregnancy activey Increasing the gestational age, improving the matur ity of fetal lung would decrease the morbidity of severe RDS and improve the pe rinatal outcome effectively.

AIM:To investigate the effects of tanshinone IIA ( Tan) on cardiac action potential in rats with a-cute cerebral ischemia (ACI).METHODS:ACI was established in rats accordingly .Animals were divided into 3 groups:sham group, ACI group, and ACI with Tan treatment group .The defect of neural function in each group was graded , elec-trocardiogram was measured , monophasic action potential was recorded , and the levels of cardiac troponin I ( cTnI ) and creatine kinase-MB ( CK-MB) were examined by commercially available kits .RESULTS:Compared with ACI group , Tan significantly decreased the scores of the defect of neural function , reduced the incidence and duration of the abnormalities in electrocardiogram , prolonged the effective refractory period , action potential duration at 50%repolarization and action po-tential duration at 90%repolarization, and inhibited the elevation of cTnI and CK-MB levels.CONCLUSION:Tan main-tains a stable heart rhythm in ACI rats , which may be related to its protective effect on ischemic myocardium .

ObjectiveTo compare the injury to hepatocarcinoma and normal liver tissues following ischemia and reperfusion. MethodsThe hepatocarcinoma(HC) models were established by injectin of VX2 tumor (tissue) suspension fluid into the left-middle lobe of liver of rabbits. After the models were established, the models left hepatic vessels were occluded for 60 minus, then followed by various intervals of reperfusion, and the levels of superoxide dismutase (SOD) and malondialdehyde (MDA) were measured, and apoptotic (changes) in the hepatocarcinoma and normal hepatic tissues were observed by means of HE staining and TUNEL method. ResultsThe concentration of SOD decreased significantly in hepatocarcinoma tissue and reached the lowest point at 1h after ischemia and reperfusion (64.594.97NU/mgprot), then gradually rebounded, but, at 7d after reperfusion, it still retained at a lower level(121.126.88NU/mgprot) than that before reperfusion. In contrast with normal liver tissues,the concentration of MDA decreased in the hepatocarcinoma tissues following ischemia and reperfusion, the changs of MDA were similar to SOD. The apoptotic cells in hepatocarcinoma tissues increased to the highest point at 1 day following reperfusion, but 7d after reperfusion, the number of positive cells were more than before reperfusion, and the apoptotic rate was higher in (hepatocarcinoma) tissues compared with the normal liver tissues. ConclusionsIn comparison with normal liver tissue, hepatocarcinoma tissue is more suscepible to the injury of ischemia and reperfusion.

Objective To study far femoral malignant bone tumor salvage limb treatment with allogeneic heterotopic half-joint transplantation and the use of GSH nail Methods Eleven cases with far femoral malignant bone tumor were administered tumor resection,transplanted with allogeneic heterotoeic bone half-joint,and fixed with GSH nail Results Nine out of the 11 cases were followed up.None of them had infection,rejection or heterotopic bone fracture.All of them can move with walking after 6 months.The effects of surgery was rated as excellent for 3 cases,fine for 4 cases andbad for 2 cases according to Mankin's rating criteria.Conclusion Salvage limb treatment for bone tumor can improve patients' quality of life.Allogeneic heterotopic bone,with rich source and acceptable joint surface,is a good graft for the treatment of bone defect after bone tumor resection.

The co-existence of physician-patient disharmony and the high rate of cesarean section suggest that there must be some internal relationship between the two medical phenomena.The physician-patient disharmony not only increases the rate of non-indicative cesarean section,but also leads to artificial expansion of the cesarean section indicators.Building up a harmonious physician-patient relationship is an important safeguard to reduce the rate of cesarean section and protect the benefits of mothers and infants.

OBJECTIVE:To establish an HPLC method to determine the content of main component in idrocilamide ointment. METHODS: The samples were separated on HP-ODS column with column temperature maintained at room temperature. The mobile phase consisted of methanol-water (65∶35) with a flow rate of 1.0 mL?min-1. The detection wavelength was set at 276 nm and the injection volume was 20 ?L. RESULTS: The linear range of idrocilamide was 0.05～4 ?g?mL-1,the average recovery rate was 99.97% (RSD=0.80%). CONCLUSION: This method is sensitive,accurate and applicable for the determination of the content of main component in idrocilamide ointment.

Changes of free amino acids in plasma and brain of rats with fulminanthepatic failure were measured with a Beckman 6300 amino acid analyzer.Results indicat-ed that as hepatic encephalopathy developed, concentrations of almost all amino acids inplasma and brain were increased.The levels of most amino acids in brain showed no cor-relation with their changes in the plasma.However,changes of concentrations of theseamino acids in brain that were related to the development of hepatic encephalopathy wereclosely correlated with their concentrations in plasma.Not any correlations between chan-ges of any amino acids in plasma and brain were found in normal control rats.Theseresults suggested that amino acid transport mechanisms appeared to be specific in the blo-cd-brain barrier during fulminant hepatic failure,a phenomenon that could contribute tothe neural disturbance of hepatic encephalopathy.

Objective To investigate the expression of Vav1 in gastric cancer(GC), and analyze its potential relevance to clinicopathological characteristics and prognostic significance in GC patients. Methods The mRNA expression level of Vav1 was detected by real-time quantitative PCR in GC cell lines (HGC-27,SGC7901 and MGC803) and normal gastric mucosa cell line (GES-1). In addition, the protein expression of Vav1 was analyzed by immunohistochemistry in formalin-fixed samples from 105 GC patients. The associations between clinical pathological features and Vav1 protein expression were evaluated in GC patients. Kaplan-Meier method and Cox regression analysis were used to analyze the related factors influencing the prognosis of GC. Results The mRNA expression levels of Vav1 were significantly higher in GC cell lines (HGC-27, SGC7901, MGC803) than those in normal gastric mucosa cell line (GES-1, P<0.05). The positive expression of Vav1 in GC tissues was correlated with diameter of tumor and lymph node metastasis (P<0.05). The univariate analysis showed that size of tumor, degree of differentiation, serosal invasion, lymph node metastasis and Vav1 expression were significantly related with prognosis of GC (P<0.05). Results of Cox regression showed that tumor invasion depth (HR=2.764, 95%CI 1.316-5.817, P=0.007), lymph node metastasis (HR=1.298, 95%CI 1.098-1.534, P=0.002) and Vav1 expression (HR=2.577, 95%CI 1.066-3.946, P=0.006) were the risk factors affecting prognosis of patients with gastric cancer. Conclusion Vav1 performs important role in the aggressiveness of GC, and Vav1 may serve as a novel prognostic factor in GC.

Focal adhesion kinase( FAK) ,a cytoplasmic non-receptor protein tyrosine kinase,serves as both a molecular scaffold and a mediator participating in multiple signal transduction pathways.FAK is involved in tumor cell survival,proliferation,migration and metastasis.Recent studies have shown that FAK is expressed in many tumor cells.Currently,FAK has been regarded as a potential target for cancer therapy.This review is to summarize the relationship between FAK and tumor progression.

OBJECTIVE:To explore the method of pharmaceutical care which influence the effect of the treatment for diabetes mellitus at home. METHODS:163 patients with diabetes mellitus in community who received treatment at home were selected, and then randomly divided into control group (58 cases),family visit group (51 cases) and group education group (54 cases);control group were given pharmaceutical intervention with traditional dispensing window;family visit group received door-to-door service or telephone follow-up service;group education group were given collective education and interactive way. The latter two groups were all given regular drug education,and the advisory desk was set up and provided consulting services about medication knowledge. RESULTS:After providing pharmaceutical care,the regular medication rate of 3 groups were 43.1%,64.8% and 78.4%;medication adherence were 51.7%,70.4% and 82.4%;the rates of blood glucose at target level were 29.3%,48.1% and 54.9%;the rates of glycosylated hemoglobin at target level were 13.7%,31.5% and 31.4%,ADR occurance rates were 31.0%, 13.0%,5.9%. These index of family visit group and group education group were all better than those of control group significantly (P<0.05). CONCLUSIONS:Humane pharmaceutical care can improve the quality and effect of the treatment for diabetes mellitus.