OBJECTIVETo detect the levels of miR-499 and relative proteins in hearts of mice after exercise training, and investigate the mechanism of exercise-regulative apoptosis.

METHODSMale C57BL/6 mice were randomly divided into 3 groups( n = 14): sedentary (SE), exercise training 1 (ET1) and exercise training 2 (ET2) group. SE did not do any exercise. ET1 performed swimming training for 8 weeks. ET2 performed the same work as ET1 until the 5th week. Then, mice trained twice a day until the end of training. TUNEL assay was applied to test myocardial apoptosis, RT-PCR and Western blot were used to detect miR-499 and proteins levels respectively.

RESULTSCompared with SE, stress in ET1 failed to affect apoptotic index (AI) and miR-499-CaN-Drp-1 pathway (P > 0.05). In contrast, exercise load in ET2 increased miR-499 level, decreased Drp-1 level and AI with statistical significance respectively (P < 0.05), but neither CaN expression nor CaN activity was changed significantly (P > 0.05).

CONCLUSIONSwimming training can inhibit myocardial apoptosis, and the decrease in Drp-l may be responsible for the reduced myocardial apoptosis. CaN, the upstream protein, does not participate in exercise-regulative apoptosis.

Animals ; Apoptosis ; Dynamins ; metabolism ; Heart ; Male ; Mice ; Mice, Inbred C57BL ; MicroRNAs ; metabolism ; Mitochondria, Heart ; physiology ; Myocardium ; pathology ; Physical Conditioning, Animal ; Swimming

Objective To study the clinical characteristics of neonatal pertussis.Method From January 2011 to December 2015,clinical data of newborns with pertussis treated in our hospital were retrospectively analyzed,including the general information,clinical manifestations,laboratory examinations,treatment and prognosis.Result A total of 68 newborns with pertussis were found during the study period,including 1 case in 2011,5 cases in 2012,1 case in 2013,18 cases in 2014 and 43 cases in 2015.The time needed for diagnosis was 7 to 35 days after onset of symptoms,and 80.9％ (55/68) in 14 days.The predominant manifestation was paroxysmal cough (68 cases,100％).Other common symptoms included flushing in 45 cases (66.2％) and cyanosis with coughing in 40 cases (58.8％).The uncommon symptoms included whooping cough (20 cases,29.4％),wheezing (10 cases,14.7％),fever (3 cases,4.4％) and apnea with decreased heart rate during cough (2 cases,2.9％).12 patients (17.6％) had elevated peripheral white blood cells or lymphocytes.The clinical manifestations didn't disappear despite erythromycin therapy,and the whooping cough continued for 12 to 42 days during the course of disease.22 cases (32.4％) had complications,including pneumonia in 12 cases,myocardial damage in 10 cases,heart failure in 2 cases,respiratory failure in 1 case,atrial tachycardia in 1 case and lung consolidation in 1 case.Conclusion Neonatal pertussis is not uncommon and has a tendency to increase year by year,and it's challenging for early diagnosis.The patients who have a whooping cough without fever should be considered of pertussis until otherwise ruled out.Leukocyte and lymphocyte count are of little value in the diagnosis of this disease.

Primary hepatic carcinoma (PHC) is one of the most common cancers worldwide and is the leading cause of death in China. Multidisciplinary treatment is widely accepted as the way to improve the prognosis of PHC, and non-surgical therapy now plays a more and more important role. The purpose of this article is to review the current status and progress of non-surgical treatments of PHC, such as TACE, local ablation, radiotherapy and systemic therapy.

Objective To investigate the value of transvaginal ultrasound in the diagnosis of endometrial polyps. Methods One hundred and ten patients diagnosed as endometrial polyps by transvaginal ultrasound and undergone operative hysteroscopy and histologic examination, were analyzed retrospectively.Results Among 110 cases 75 cases (68.2%) were accurately diagnosed by transvaginal ultrasound,while 35 cases (31.8%) were misdiagnosed.The diseases misdiagnosed included endometrium hyperplasia,intrauterine adhesions and submucousmyoma.Conclusions Combining clinical symptoms with ultrasonogram characters and optimal time of detection can reduce the misdiagnostic rate of endometrial polyps.

Objective To compare the effectiveness of Pregabalin given by fixed dose method and flexible dose method on treating central post-stroke pain.Methods Patients with central post-stroke pain were consecutively enrolled and randomized into fixed group and flexible group.The patients in fixed group were given pregabalin 300 mg per day for 8 weeks.The patients in flexible group were given pregabalin as follows:150 mg per day for 2 weeks, 300 mg per day for 2 weeks and 450 mg per day for 4 weeks.The primary outcome was numerical rating scale ( NRS) . The secondary outcomes included adverse effects, hospital anxiety and depression scales ( HADS ) and Athens insomnia scale ( AIS) .Results A total of 132 participants were enrolled with 65 in fixed group and 67 in flexible group from Jul 2009 to Dec 2014.Two patients in fixed group gave up the treatment while 7 patients gave up in flexible group though no significant difference was observed.Finally, 63 patients in fixed group and 60 patients in flexible group were included into analysis.There was no significant difference on gender, age, medical history between two groups. Compared with baseline, the NRS、HADS-A、HADS-D and AIS score were significantly decreased ( all P<0.05 ) .However, there was no significant difference of these scores between the two groups. Conclusion Two regimens of pregabalin showed the similar efficiency and safety to treat CPSP, however, fixed dose seemed to be more acceptable.

Objective To explore the mechanism of imperfect recovery of the renal function after release of chronic ureteral obstruction. Methods Adult New Zealand rabbits(n=36) were randomly divided into normal control group(n=3),obstructive control group(n=3) and experimental group(n=30).The latter two groups were subjected to chronic unilateral ureteral obstruction.Two weeks later,3 rabbits were sacrificed and the others underwent ureterostomy,which were sacrificed separately following 12 hours,24 hours,3 days,7 days,14 days.The renal papilla and cortex were examined by scanning electron microscopy. Results Shortly after the release of ureteral obstruction,massive loss of brush border and ballooning of epithelial cells were noted in proximal tubules.Distal tubules and collecting tubules also showed severe ballooning of epithelial cells and normal struction were not seen.Two weeks later,many tubular components became patent with normal epithelial lining.Some of them,however,were deteriorated further in spite of the release of obstruction.Thus there was a marked tubular heterogeneity in recovery.Conclusion Further deterioration of some tubules might be one of the important pathologic basis for imperfect recovery of renal function after the release of chronic ureteral obstruction.

Objective:To explore clinical characteristics and diagnosis in aged patients with pulmonary embolism. Methods:The data of electrocardiogram (ECG),clinical characteristics and diagnosis were summarized and ana-lyzed in 56 aged patients with pulmonary embolism.Results:The most frequent symptom was exertional dyspnea (69.6%).ECG had characteristic changes in 12 patients (21.4%).All 56 patients received multi-slice spiral CT pulmonary arteriography,and thrombus within pulmonary artery was found in all patients (100%);38 cases re-ceived nuclide lung ventilation perfusion scanning,the results were 32 cases (84.2%)with mismatching pulmonary perfusion and ventilation imaging;Ultrasound examine found there were phlebothrombosis in deep vein of lower limb of 26 cases (46.4%).Misdiagnosis:The 26 cases (46.4%)were misdiagnosed.A total of 14 cases (53.8%) were misdiagnosed as respiratory system diseases,including eight cases of infectious shock and six cases with pulmo-nary infection;12 cases (46.2%)were misdiagnosed as circulatory system diseases,including five cases of acute cor-onary syndrome,four cases of heart failure and three cases of cardiogenic shock.Conclusion:The most frequent symptom is exertional dyspnea in aged patients with pulmonary embolism;ECG has characteristic changes only in few patients;the pulmonary arteriography is major diagnosis method.

Animals ; Annexin A2 ; chemistry ; genetics ; metabolism ; physiology ; DNA Replication ; Endocytosis ; Exocytosis ; Humans ; Neoplasm Invasiveness ; Neoplasm Metastasis ; Neoplasms ; metabolism ; pathology ; Prognosis ; Tissue Plasminogen Activator ; metabolism ; physiology

Objective:To explore the impact of continuous nursing education on the quality of life, psychological state and satisfaction of patients with inflammatory bowel disease based on micro-video.Methods:In chronological order, 83 patients with inflammatory bowel disease (hospitalized from January to June, 2019 in Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology) were divided into two groups, the control group with 42 cases (discharged from the hospital from January to March, 2019) carried out regular telephone continuing nursing education and the intervention group with 41 cases (discharged from hospital from April to June, 2019) were provided the continuous nursing education in the form of telephone plus micro-video. Before intervention and after 3 months of intervention, quality of life, levels of anxiety and depression and nursing satisfaction were evaluated by inflammatory bowel disease questionnaire (IBDQ), Self-rating Anxiety Scale(SAS), Self-rating Depression Scale(SDS), digital table method and compared between the two groups.Results:There was no significant difference in quality of life, levels of anxiety and depression before intervention between the two groups( P>0.05). After 3 months of intervention, the scores of IBDQ, SAS, SDS were 155.1±23.7, 58.7±15.1, 59.5±11.1 in the intervention group and 115.7±21.7, 71.5±16.7, 72.8±12.0 in the control group, the differences between the two groups were statistically significant ( t values were 7.910, 3.642, 5.223, P<0.01). The nursing satisfaction score was 89.32±6.34 in the intervention group and 81.45±7.21 in the control group, the difference between the two groups was statistically significant ( t value was -5.284, P<0.01). Conclusions:Continuous nursing education in the form of micro-videos can increase the quality of life of the inflammatory bowel disease patients, improve mental state and their nursing satisfaction in further.