OBJECTIVETo study the relationship between the genetic diversity and chemical variation of Cinnamomum migao.

METHODISSR marker technique was used to research the genetic structure of 9 population, GC-MS was used to analyze the main ingredients of the volatile oil in C. migao.

RESULTThe analysis on the main ingredients of the volatile oil showed that there were significant or extremely significant differences in 9 populations. The minimum variation index of population was Yunnan Funing and the maximum variation index of population was Guangxi Yueye. ISSR marker analysis showed that the average of polymorphic loci percentage (P) was 42.41%, expected heterozygosity (H) was 0.181 0, Shannon's information index (I) was 0.293 8, the Nei's genetic diversity (H(s)) in the group was 0.188 9, genetic differentiation index (G(st)) was 2.269 1. The relationship between the genetic diversity and chemical variation showed that there was no significant correlation between the main ingredients of the volatile oil and 4 indexes of genetic structure of C. migao.

CONCLUSIONThe genetic diversity of C. migao was relatively high at the population levels, while it is low within the population levels, the relationship between chemical variation and genetic diversity was not obvious, that may indicate that other factors causes the chemical variation of C. migao.

China ; Cinnamomum ; chemistry ; genetics ; DNA ; genetics ; isolation & purification ; Gas Chromatography-Mass Spectrometry ; Genetic Markers ; Genetic Variation ; genetics ; Oils, Volatile ; analysis ; chemistry ; isolation & purification ; Plant Oils ; chemistry ; Polymorphism, Genetic ; genetics ; Repetitive Sequences, Nucleic Acid ; genetics

OBJECTIVETo compare the constituents of the volatile oil in Cinnamomum migao from different regions of southwest in China in order to evaluate the quality of C. migao.

METHODGC-MS was employed to analyze the constituents of the volatile oil in C. migao.

RESULTThe volatile oil compositions of C. migao collected from 27 of cultivation regions were obviously different. Based on the chemical differences of the volatile oil compositions, C. migao was divided into four chemotype, they were eucalyptol, eucalyptol -cyclohexene, eucalyptol -alpha-terpineol, and eucalyptol -sabinene. The eucalyptol-type was cultivated in Luodian, guizhou province and Funing regions, Yunan province. The eucalyptol-cyclohexene-type was cultivated in Zhengfeng and Wangmo regions, Guizhou province. The type of eucalyptol, eucalyptol -sabinene and eucalyptol -alpha-terpineol were cultivated in Ceheng and Libo regions, Guizhou province.

CONCLUSIONCombined with the geographical distribution, It is indicated that the volatile oil compositions in fruit of C. migao may have some relations to the specie itself characteristics and different elevations environment.

Cinnamomum ; chemistry ; Drugs, Chinese Herbal ; chemistry ; Fruit ; chemistry ; Oils, Volatile ; analysis