Objective To test the hypothesis that advanced glycosylation end products(AGEs) increase cellular inflammation in atherosclerotic plaques. Methods Fifty rabbits were randomly divided into five groups. Hypercholesterolemic (0.5% cholesterol in diet) rabbits received repeated intravenous injection of either AGEs modified rabbit serum albumin (AGEs-RSA ) (group A) or unmodified RSA (group B) for 10 weeks. Rabbits treated with either hypercholesterolemic diet (group C)or with a normal diet(group D) or with a normal diet, and intravenous injection of AGEs-RSA (group E) were served as controls. Aortas were harvested at the 10th week, and lipid deposition was quantitated by oil red 0 staining. Macrophage (RAM-11 positive cells) and T lymphocyte (CD43 positive cells) infiltration, smooth muscle cell(?-actin positive cells) migration and proliferation were determined by using immunohistochemical staining and image-analysis techniques. Results Atherosclerotic plaques could be found in animals fed with hypercholesterolemic diet.Lipid deposition in plaque was significantly higher in group A (71.86%?8.3%) than those in group B (53.76%?3.72%)and group C (56.67%?9.2%). Infiltrations of macrophage[ (23.1?8.5)/0.01 mm2]and T lymphocyte[ (15.1 ? 3.8)/0.01 mm2]as well as migration and proliferation of smooth muscle cell [ (19.2?5.7)/0,01 mm2] in atherosclerotic lesions were significantly increased in animals treated with hypercholesterolemic diet and received injection of AGEs-RSA (group A) when compared with group B [macrophage (14.4? 5.9)70.01 mm2; T lymphocyte (9.1?2.6)/0.01 mm2; smooth muscle cell (12.9?3.8)/0.01 mm2]and group C[macrophage (15.4?4.4)/0.01 mm2; T lymphocyte (10.5?2.2)/0.01 mm2, smooth muscle cell (13.8?3.9)/0.01 mm2]. Neither plaque nor a cellular inflammation was found in animals fed with normal diet (group D)and in those received repeated injections of AGEs-RSA (group E). Conclusion AGEs increase cellular inflammation in atherosclerotic plaques and may accelerate formation of atherosclerosis in AGEs associated diseases.

Objective To investigate the blood pressure control effect of captopril sustained-releasetablets based combination, antihypertensive therapy on hospitalized high-risk patients with hypertension in high altitude region. Methods According to the blood pressure,risk factors and combined target organ damage,331 hospitalized patients with essential hypertension were divided into 2 groups and accepted different treatment:low-risk group had monotherapy (n=102) , and night-risk group had captopril sustained-release-tablets based combination antihypertensive therapy (n =229). The discharge blood pressure,extent of SBP/DBP decrease and the compliance rate of discharge blood pressure of two groups were compared. Results The extent of SBP/DBP decrease in hight-risk group was significantly greater than low-risk group (SBP [36. 83 ± 22. 23] mm Hg vs.[28. 74 ±18.71] mm Hg,t=-3. 207,P <0. 05;DBP[22. 04±13. 57]mm Hg vs. [17. 98 ± 13.63] mm Hg,t =-2. 509, P < 0.05). The average discharge blood pressure in both groups reach the standard criterion, but no significant difference was observed between the two groups (SBP [125. 62 ± 14. 74] mm Hg vs. [122. 28 ±13.13]mmHg,t=-1. 962,P>0. 05;DBP[80. 67 ±9. 82]mm Hg vs. [78. 40 ±9. 97]mm Hg,t =-1.910,P > 0. 05). Furthermore we found no significant difference in the control rate of blood pressure between high-risk and low-risk group (72. 06% vs. 71. 57% , x2 = 0.928, P > 0. 05). Conclusion The captopril sustainedrelease-tablets based combination antihypertensive therapy is a reliable treatment in high-risk patients with hypertension from high altitude region,which shows satisfying blood pressure control rate.

Objective:To explore the role of multiple mediating effects on scientific research creativity of nursing postgraduates.Methods:In the study, 127 nursing postgraduates from three affliated hospitals of Chongqing Medical University were selected by convenience sampling. The investigation scale included general information questionnaire, tutor support scale, nursing scientific research self-efficacy scale, autonomy motivation scale and scientific research creativity scale. The questionnaire of nursing postgraduates was collected through the Sojump platform, and the data of 127 postgraduates were effectively collected in strict accordance with the inclusion and exclusion criteria. The difference analysis and correlation analysis were carried out by SPSS.Results:The correlation analysis showed that nursing postgraduates' scientific research self-efficacy and autonomy motivation were significantly positively correlated with tutor support and scientific research creativity ( P<0.05). Multiple linear hierarchical regression analysis showed that nursing research self-efficacy and autonomy motivation played a partial mediating role between tutor support and scientific research creativity ( P<0.05), in which the mediating role explained 13.1% and 4.2% of the variation respectively. The structural equation model further verified the role of multiple mediating effects on the scientific research creativity of nursing postgraduates. Conclusion:The scientific research creativity of nursing postgraduates is closely related to tutor support, scientific research self-efficacy and autonomy motivation. Tutors can enhance nursing postgraduates' scientific research self-efficacy and autonomy motivation by improving the level of tutor support, so as to improve the scientific research creativity of postgraduates.

Objective:To investigate the status of continuous care for children with chronic diseases in public hospitals in Chongqing, so as to provide references for improving the quality of continuous care for children with chronic diseases.Methods:This study is a cross-sectional study. From April to May 2022, a total of 66 managers from 66 departments of 50 hospitals in Chongqing were selected as the research objects by the purposive sampling method. The Implementation of Transitional Care for Children's Chronic Diseases in Medical and Health Institutions Questionnaire was used to investigate the implementation, management status and managers' evaluation of continuous care for children with chronic diseases. A total of 66 questionnaires were distributed in this study, and 66 valid questionnaires were collected, with an effective recovery rate of 100.00% (66/66) .Results:Among 50 hospitals, 29 provided transitional care items for children with chronic diseases. The continuous care of children with chronic diseases had many problems, such as single content and form, low utilization rate of information platform, no standardized evaluation system and quality management program, and low satisfaction of continuous care for children with chronic diseases.Conclusions:Public hospitals in Chongqing have launched continuous care for children with chronic diseases, but the breadth and depth are limited, and there are still shortcomings in form, content, and effectiveness evaluation. In the future, it is necessary to further expand the content and form of continuous care for children with chronic diseases, establish standardized continuous care evaluation indicators, and improve the quality of continuous care for children with chronic diseases.