The present study retrospectively analyzed 23 patients with ulnar fracture or bone nonunion who received treatment in the Department of Orthopedics, Second Affiliated Hospital of Soochow University between August 2001 and December 2008. These patients comprised 16 males, 7 females, and averaged 41.8 years old (range 20-72 years old). Of them, 14 had single ulnar fracture, 6 had monteggia fracture, 19 had fresh fracture, 1 had obsolete fracture, and 3 presented with bone nonunion and plate breakage following ulnar fracture. All patients received interlocking intramedullary nailing. Internal fixation time and the mean time to fracture healing were recorded. The function of nailed forearm was evaluated using Anderson criteria. All 23 patients were followed up in terms of intra- and post-operative complications for a period of 6 months -7.3 years. Following Hertel classification criteria, bone union occurring at a mean of 14.7 weeks was found in 23 patients. No intramedullary nail loosening, fragmentation, or incision infection was observed. Twenty patients had intramedullary nails removed but did not present recurred fracture. According to the Anderson evaluation criteria regarding forearm functions, the results were excellent in 22 patients and satisfactory in 1 patient. These findings indicated that interlocking intramedullary nailing for treatment of ulnar fracture provides less trauma, short recovery period, and low recurrence. For these advantages, it is suitable for treatment of ulnar shaft fracture, multi-segment ulnar fracture, bone defects, bone non-union, and the fractures failed after compression plating; in addition, it is a better choice in treating ulnar fracture in patients with severe soft tissue injury or osteoporosis.

[Objective]Ulnar fractures fixed by interlocking intramedullary nails or dynamic compression plates were tested to compare their biomechanical function,in order to provide the theoretical basis for clinical practice.[Method]In the experiment,12 pieces of fresh ulnars were used to produce middle-part transverse fracture models;which were fixed by interlocking intramedullary nails or six-hole 3.5 mm dynamic compression plates seperately.The diameter of intramedullary nail was 4mm,and the length was 200-230mm.The specimen was set on the MTS test machine.The rigidity and strength of ulnar fractures fixed by interlocking intramedullary nails were compared with those of ulnar fractures fixed by six-hole 3.5 mm dynamic compression plates in the anti-axial test,anti-bending test,anti-torsional test.[Result]In the anti-axial test,anti-bending test and anti-torsional test,the rigidity of ulnar fractures fixed by interlocking intramedullary nails was 450.00?38.42 N/mm,45.64?5.24 N?cm/Deg,11.42?1.21N?cm/Deg in sequence;while the rigidity fixed by dynamic compression plates was 405.40?29.26 N/mm,41.00?4.78 N.cm/Deg,10.05?1.32 N?cm/Deg accordingly.Burdened 1000N axial pressure,the displacement of interlocking intramedullay nail fixing specimen was 2.20?0.11 mm,and the compression plate fixing specimen was 2.48?0.15 mm.Given a 5 N?M bending burden,the maximum radial bending degree of interlocking intramedullay nailfixing specimen was 3.25?0.15 mm,which was 3.60?0.21 mm of compression plate fixing specimen.In the anti-torsional test,the interlocking intramedullay nail and compression plate fixing specimen could burden 2.40?0.13 N?M and 1.90?0.10 N?M respectively.The experimental data were analyzed by software SPSS.10,which came to a distinguished difference by t-test(P

Objective To find out the relationship between kidney- yang- deficiency syndrome and the Th1/Th2 expression, and to investigate the effect of kidney- yang warming therap yon the Th1/Th2 expression. Methods Kidney- yang- deficiency rat models were firstly induced by adenine and then were sensitizated by inhalation of ovalbumin to establish allergic rhinitis (AR)models .The treatment group was given Jinkui Shenqi pill. After treatment , the changes of behavior , secretion of nasal cavity and nasal mucosa were observed. The levels of Th1 cytokines IFN- ? , IL- 2 and Th2 cytokines IL- 4 , IL- 5 in serum were determined by ELISA. Results The behavioral score in AR group and kidney- yang- deficiency AR group was higher than that in the treatment group and normal control group ( P

BACKGROUND: Autogenous bone has been used in cervical vertebra graft bone fusion in earliest stage and at most. However, its source is limited, simultaneously, induced many complications such as infection, hemorrhage and postoperative pain in the donor bone region. Recently, above-mentioned complications were avoided or reduced with the usage of new graft bone fusion material. OBJECTIVE: To compare clinical efficacy using MC+~R combination of autogenous bone or calcium sulfate artificial bone in antador cervical fusion.METHODS: A total of 26 patients (34 levels) with cervical spondylotic myelopathy underwent anterior cervical discectomy and cervical intervertebral fusion from January to December 2008. Anterior cervical oblique cut was 3.0-4.0 cm. The endplate were preserved after the cervical intervertebral disc and the posterior longitudinal ligament were removed. Autogenous bone group was filled with autogenous bone. Calcium sulfate artificial bone group was filled with Wdght's Osteoset artificial bone. Anchoring clip was implanted between the cervical vertebrae. Every patient had a short neck incision was assessed with X-ray, JOA grade and Odom's evaluation scale.RESULTS AND CONCLUSION: The two groups of 26 patients (34 segments)were followed up. The JOA score of postoperation was no significant difference between the two groups. According to the Odom's evaluation scale, the excellent and good rate of calcium sulfate group was higher than autogenous bone group, but there was not statistical significance (P>0.05). The fusion rate of autogenous bone group was higher than calcium sulfate group at 3 and 6 months, but the fusion rate of two groups were 100% at 12 months. Although the calcium sulfate group at 6 months, lordosis angle lost more than 0.4°than the autogenous bone group,but no significant statistically between the two groups (P>0.05). MC+ combination of autogenous bone or Calcium sulfate had the same clinical efficacy in the treatment of cervical spondylotic myelopathy, but the calcium sulfate artificial bone could be effectively avoided the complications of donor site.

AIM: To investigate the genes differential expression in cortex during rat focal cerebral ischemia. METHODS: cDNA microarray chips containing numerous cDNAs were used to investigate the gene expression pattern between samples of focal cerebral ischemia and sham-control operation rats. RESULTS: Two hundred and eleven genes differentially expressed were screened out, among these genes, up-and down-regulated genes were 199 and 12, respectively. CONCLUSIONS: The analysis of gene expression pattern of focal cerebral ischemia based on cDNA microarray can realize high-throughput screening of the genes associated with the focal cerebral ischemia. The differential expression of genes may be related to the pathogenesis of focal cerebral ischemic diseases.

Nerve growth factor (NGF) can promote the regeneration of peripheral nerve as well as contraction and reepithelization of wound. We constructed a bioengineered dermis containing microencapsulated NGF-expressing NIH-3T3 cells and study the effect of the microencapsule to the bioengineered dermis and seed cells. NGF gene was transfected into NIH-3T3 cells and enclosed into alginate-poly-L-lysine-alginate (APA) microencapsulation and cultivated in vitro. Content of NGF in microencapsules culturing supernatant was measured by enzyme linked immunosorbent assay (ELISA) method. These microencapsules were co-cultured with epidermic cells and fibroblast cells. Bioengineered dermis was constructed with NGF-expressing micorencapsules as seed cells using tissue engineering method. NIH-3T3 microencapsules, empty microencapsules, normal culture media were control groups. After one week culture, the characteristics of the dermis were described by MTT test, the content of hydroxyproline (HP), HE staining and ultrastructure photograph. We found the NGF-expressing microencapsulates can secret NGF steadly after cultured 8w in vitro, promot the proliferation of epidermic cells and secret collagen of fibroblast cells. These functions can maintaine in bioengineered dermis. So NGF-expressing NIH-3T3 microencapsulates can promote the quality of bioengineered dermis.
Alginates ; chemistry ; Animals ; Biocompatible Materials ; chemistry ; Cell Proliferation ; Dermis ; cytology ; Gene Expression Regulation ; Mice ; NIH 3T3 Cells ; Nerve Growth Factor ; genetics ; Polylysine ; analogs & derivatives ; chemistry ; Skin Physiological Phenomena ; Tissue Engineering ; methods ; Transfection ; methods

FRMD6, a member of the 4.1 ezrin-radixin-moesin domain-containing protein family, has been reported to inhibit tumor progression in multiple cancers. Here, we demonstrate the involvement of FRMD6 in lung cancer progression. We find that FRMD6 is overexpressed in lung cancer tissues relative to in normal lung tissues. In addition, the enhanced expression of FRMD6 is associated with poor outcomes in patients with lung squamous cell carcinoma (n = 75, P = 0.0054) and lung adenocarcinoma (n = 94, P = 0.0330). Cell migration and proliferation in vitro and tumor formation in vivo are promoted by FRMD6 but are suppressed by the depletion of FRMD6. Mechanistically, FRMD6 interacts and colocalizes with mTOR and S6K, which are the key molecules of the mTOR signaling pathway. FRMD6 markedly enhances the interaction between mTOR and S6K, subsequently increasing the levels of endogenous pS6K and downstream pS6 in lung cancer cells. Furthermore, knocking out FRMD6 inhibits the activation of the mTOR signaling pathway in Frmd6^-/- gene KO MEFs and mice. Altogether, our results show that FRMD6 contributes to lung cancer progression by activating the mTOR signaling pathway.