OBJECTIVE:To establish a simple and rapid method for the quality control of Tianxing dingxian capsules.METHODS:Pheretima,Borneolum Syntheticum,Salvia miltiorrhiza,Ramulus Uncariae Cum Uncis,Succinum,Calculus Bovis Artifactius,Radix et Rhizoma Glycyrrhizae,Ginsenoside Rg1 and Paeonia lactiflora were identified by TLC.The content of Salvianolic acid B in the Tianxing dingxian capsules was determined by HPLC.RESULTS:The TLC spots were clear,well-seperated yet without interference from negative Samples.The linear range of Salvianolic acid B was 0.061 44～1.92 ?g(r=0.999 8),and its average recovery was 99.33%(RSD=1.76%,n=9).CONCLUSION:The method can be used for the rapid quality control of Tianxing dingxian capsules.

AIM: To establish a method for the quality control of Xiao'er Ganmao Granules(Flos Chrysanthemi, Radix Cynanchi Atrati, Cortex Lycii, etc.). METHODS: Radix Cynanchi Atrati, Cortex Lycii and Flos Chrysanthemi were identified by TLC. The forsythin content in Xiao'er Ganmao Granules was determinated by HPLC. RESULTS: The methodological study showed that a good linear correlation for the forsythin existed in a range of 4.76～588ng. The average recovery of forsythin ( n =9) was 98.7% and RSD was 1.7%. CONCLUSION: The method is simple, accurate and with good repeating and high precision, and can be used for quality control of Xiao'er Ganmao Granules.

BACKGROUND:Interlocking intramedullary nailing exhibits unique superiority in treatment of multi-segment radial fracture,bone nonunion,and osteoporotic fracture. However,distal interlocking screw placement would injury the deep branch of radial nerves. OBJECTIVE:To analyze the anatomical characteristics of the deep branch of radial nerves during radial interlocking intramedullary nailing. DESIGN,TIME AND SETTING:An observational measurement was performed at the laboratory of Department of Orthopedics,Second Affiliated Hospital of Soochow University between November and December 2002. MATERIALS:A total of 44 pieces of fresh adult cadaver forearm specimens were provided by Department of Anatomy,Soochow University,China. A vernier caliper was purchased from Henan Yuanyang Zhenhua Instrument Factory,China. METHODS:The deep branches of radial nerve of 44 fresh forearm specimens were exposed. The lateral epicondyle of humerus was joined to the Lister tubercle of radius. The line passed through 6 horizontal planes,which were as follows in sequence:0,1.0,1.5,and 2.0 cm lower than the articular surface of radial head respectively,the horizontal planes of the deep branch of radial nerve entering the supinator and winding across the radius. The crossed points were named points A,B,C,D,E,and F accordingly. MAIN OUTCOME MEASURES:The distance between the deep branch of radial nerve and fixed points B,C,and D was measured separately when the forearm was kept in pronation,neutral and supination positions. RESULTS:The distance between the deep branch of radial nerve and fixed points B,C,D became nearer and nearer in the sequence of B,C,and D. With the forearm in pronation,neutral and supination positions in sequence,each fixed point became farther and farther from the deep branch of radial nerve. CONCLUSION:When interlocking intremedullary nailing is used to fix radius,it is relatively safe to keep the forearm in neutral and flexion positions,and drilling and insertion of distal interlocking screw at 1.5 cm lower the articular surface of radial head from the posterolateral to anteromedial approach should be selected.

OBJECTIVE:To establish a method for the simultaneous determination of ethambutol(EMB)and isoniazid(INH) in children’s plasma. METHODS:After precipitated with acetonitrile,plasma samples were determined by UPLC-MS/MS method. Using lamivudine as internal standard,Waters ACQUITY? UPLC HSS T3 column was adopted with mobile phase consisted of ace-tonitrile-water(containing 0.05% formic acid and 15 mmol/L ammonium formate)in gradient mode at the flow rate of 0.2 ml/min. By ESI,positive ion detection was conducted in MRM mode. The monitoring transition ion-pair was m/z 205.2→116.0 for EMB, m/z 138.1→121.1 for INH and m/z 230.2→111.9 for internal standard. RESULTS:The linear range of EMB and INH was 10-5 000 ng/ml and 50-5 000 ng/ml,respectively;the limits of quantitation were 10 and 50 ng/ml;RSDs of inter-day and intra-day were all lower than 15% and relative recoveries were 98.7%-105.5%. Plasma concentrations of EMB and INH in 4 children with tuberculo-sis were 94.5-99.7 and 55.1-80.9 ng/ml. CONCLUSIONS:The method is rapid and sensitive,which suitable for children’s plasma concentration monitoring of EMB and INH and pharmacokinetic study.

OBJECTIVE: To establish the preparation process and the quality control standards for Jinhuanglidan Granules (JHLD). METHODS: Rheum Officinale, Rhizoma Coptis, Radix Paeoniae Alba and Radix Scutellariae were identified by TLC. The content of berberine hydrochloride in the preparation was determined by TLCS. RESULTS: Rheum Officinale, Rhizoma Coptis, Radix Paeoniae Alba and Radix Scutellariae could be identified by TLC. Berberine hydrochloride showed a good linearity in the range of 0 .05 985ug- 0. 29 925ug, r = 0. 9 996(n = 5). The average recovery was 99. 64%, RSD = 2. 54%. CONCLUSIO- N: This method is simple, accurate and specific. It can be used for the quality control of JHLD.

OBJECTIVE: To facilitate separate-store management and cold-chain management for hospital drugs. METHODS: The temperature division of separate-store management of hospital drugs was analyzed. The cold-chain management of cold-storage and freeze-storage drugs was discussed. RESULTS & CONCLUSIONS: During the storage period of hospital drugs,the temperature of cold-storeroom should be kept at 2～8 ℃,that of shady and cool storeroom at 0～20 ℃,and that of the normal temperature storeroom at 0～30 ℃. In the cold-chain management,great importance should be attached to several key steps such as to standardize the transport vehicles,set up stores for the turnover of drugs,and to clarify the operational procedure and working standard. It is necessary to strengthen separate-storehouse management and cold-chain management for hospital drugs.

OBJECTIVE: To analyze the utilization of Chinese patent medicines in our hospital to provide scientific basis of rational use of drugs in clinic.METHODS: Data about annual consumption sum and consumption amount of Chinese patent medicines in our hospital during 2005～2009 were collected to analyze annual consumption sum,DDDs end daily cost of Chinese patent medicine statistically.RESULTS: The annual consumption sum of Chinese patent medicines showed a upward trend in our hospital during 2005～2009.The top 15 Chinese patent medicine in the list of consumption sum which took up a large proportion affected the total consumption sum.Injection was used more and more so that the ratio of its consumption sum to rank of DDDs was lower than one.CONCLUSION: Cheap and effective Chinese patent medicines play an important part in the clinic.DDDs order should be emphasized in the drug supervision and Chinese traditional medicines injection should be managed strictly.

Objective To develop a rapid ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/ MS) for determination of 11 sedative-hypnotics in human plasma. Methods The plasma samples were extracted with dichloromethane:n-hexane:acetoacetate (5∶4∶1). The separation was performed on a Waters ACQUITY UPLC HSS T3 column (2.1 mm×100 mm,1.8 μm) using the mobile phase composed of acetonitrile-0.1% ammonium solution at a flow rate of 0.2 mL?min-1 in gradient elution mode. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) via electrospray ionization (ESI) source in positive mode. Results Good linear relation was obtained over the investigated concentration range, with all correlation coefficients higher than 0.99. The limit of detection was 200 pg?mL-1 for dexzopiclone,and 10- 20 pg?mL-1 for other sedative-hypnotics. The intra- and inter-day RSD of 11 sedative-hypnotics were no more than 15.0%. The recoveries were in the range of 72.3%-108.0%, and the matrix effects were approximately 0.86- 1. 12. Conclusion The method is rapid, sensitive and reliable, and suitable for the simultaneous determination of 11 sedative-hypnotics in human plasma.

Objective To evaluate the correlation and difference of reversed phase high performance liquid chromatography (RP-HPLC) and fluorescence polarization immunoassay (FPIA) on determining serum concentration of carbamazepine.Methods Fifty serum samples were collected,both RP-HPLC and FPIA methods were employed to determine the concentration of carbamazepine.The results were analyzed by paired t test,Bland-Altman and Deming regression methods,respectively.Results The results of measuring 50 samples by the two methods showed that FPIA datas were significantly higher than RP-HPLC datas,and there was statistically significant difference(P<0.05) and poorer consistency between two methods;There was good correlation between carbamazepine concentrations determined by the two methods.Deming regression equation was CFPIA=1.195 3 CRP-HPLC-0.144 0,and Pearson correlation coefficient was 0.968 5.Conclusion Clinicians should pay more attention to the difference of carbamazepine concentration determination by different methods when carbamazepine individualized dosage regimen was adjusted according to therapeutic drug monitoring.

OBJECTIVE:To investigate the compatible stability of Calcium folinate for injection mixed with Glucose injection and Sodium chloride injection. METHODS:Referring to clinical common concentration,each 3 Calcium folinate for injection (each injection was equal to calcium folinate 100 mg)were respectively mixed with Glucose injection 250 mL or Sodium chloride injection 250 mL. At room temperature,under light or dark condition,the appearance of mixtures,pH value and the number of in-soluble particles were investigated 0,1,2,3,4,6,8,12,24,36,48 h. The contents of calcium folinate in mixtures were deter-mined by HPLC. RESULTS:Under above condition,the color of the mixtures had no change,and no gas,precipitation and turbid-ity was found;there was no evident change in pH values(RSD<2%,n=11). 0 h after mixing,there was large number of parti-cles≥10 μm in mixtures,but the number of particle was decreased as time;within 48 h,the number of particles ≥10 μm and ≥25 μm in mixtures were all in line with the standard of Chinese Pharmacopeia(2015 edition). Under the protection from light con-dition,relative contents of calcium folinate in mixtures had no significant change(RSD<2%,n=11). Under light condition,rela-tive contents of calcium folinate in mixtures decreased significantly,decreasing to 94.5%(mixed with Glucose injection) and 88.4%(mixed with Sodium chloride injection). CONCLUSIONS:Calcium folinate for injection is more stable in Glucose injec-tion,and the stability of compatibility can be affected by light conditions. After mixed with Glucose injection and Sodium chloride injection,Calcium folinate for injection should be kept away from light and used as soon as possible