Objective To determine the effect of diffusion chamber for immune isolation and the proliferation of allogenic hybridoma within diffusion chamber in mice. Methods The hybridoma cell strain was established from SP2/0 cells and the splenocytes of BALB/c mouse by PEG mediate technique. The cells were injected peritoneally into BALB/c ( n =5) and C57 BL/6 (B6, n =5) mice. Diffusion chambers with microporous membrane containing hybridoma cells were implanted peritoneally into 5 BALB/c and 5 B6 mice. Results Within two months after peritoneal injection of the cell strain, four BALB/c mice developed bloody ascites and one developed abdominal tumor, but the B6 mice developed neither ascites nor tumor. After 30 and 101 days of the chamber implantation, the chambers were coated by omentum or mesenterium in all the animals of both strain groups and freshly formed blood vessels to the chamber membrane could be observed. Within the chambers, tumor developed, but the tumor could not fully fill the inner space of the chamber during the whole observation period. Conclusion The hybridoma cells established from SP2/0 cells and the splenocytes of BALB/c mouse may carry BALB/c MHC antigen. The diffusion chambers can effectively isolate immune rejection. Freshly formed blood vessels to the chamber membrane can support the cells within the chambers.

Objective To study and investigate the detection value of noninvasive cerebral hemodynamics and serum nerve func‐tion indexes in the patients with craniocerebral injury .Methods Totally 64 patients with craniocerebral injury in our hospital from September 2013 to May 2015 were selected as the observation group ,meanwhile 64 healthy persons with the same age were selected as the control group .Then the noninvasive cerebral hemodynamics and serum nerve function indexes were compared between the two groups ,furthermore the detection results in the observation group were compared among the patients with different severity de‐grees and intracranial pressures .Results The middle cerebral artery blood flow indexes of the observation group were all higher than those of the control group ,the serum nerve function indexes were also higher than those of the control group ,and the middle cerebral artery blood flow indexes and serum nerve function indexes of the observation group had obvious differences among the pa‐tients with different severity degrees and intracranial pressures too (P< 0 .05) ,showing statistical significance .Conclusion The noninvasive cerebral hemodynamics and serum nerve function indexes have highe detection value in the patients with craniocerebral injury ,and have active clinical role for the understand of disease severity degree and intracranial pressure situation .