1.Analysis of Staphyloxanthin Biosynthesis Using Single Cell Raman Spectroscopy
Zhanhua TAO ; Junxian LIU ; Deqiang SHI ; Jian KANG
Chinese Journal of Analytical Chemistry 2016;(3):456-461
Laser tweezers Raman spectroscopy ( LTRS ) was used to study the inhibitory effect of indol on staphyloxanthin biosynthesis in Staphylococcus aureus cells and the dynamic changes of this pigment content inside bacterial cells during batch cultivation. The Raman spectra of Staphylococcus aureus cells cultivated for different time and exposed to various doses of indol were acquired. The intensity of 1523 cm-1 band was used for the quantification of staphyloxanthin, in the meantime, the pigment was measured by UV spectrometry. The experimental result showed that an excellent linear relationship existed between the intensities of Raman peak at 1523 cm-1 of bacterial cells and the pigment contents estimated by UV spectrometry, with a correlation coefficient of 0 . 9772 . The spectral data at population level as well as single cell level revealed that indol could inhibit the production of pigment in dose-dependent manner, and the pigment content in bacterial cells incubated with indol decreased by 70%. Under the batch growth condition, the pigment amount in Staphylococcus aureus cells reached the maximum value during the middle exponential growth phase ( 12 h ) and the heterogeneity of pigment content in bacterial cells within certain populations at various time points was relatively small, with RSDs of 39. 2% to 61. 1%. This investigation indicates that LTRS can be served as a reliable method for the analysis of staphyloxanthin content at single cell level.
2.Clinical and electrophysiological value of sensory tricks in cervical dystonia
Tao LI ; Chunli SONG ; Lina ZHOU ; Lu JING ; Lu ZHOU ; Zhanhua LIANG
Chinese Journal of Neurology 2017;50(9):660-664
Objective To investigate demography of sensory tricks in cervical dystonia (CD),and to discuss the relationship between sensory tricks and the function of sensorimotor integration of cerebral cortex,meanwhile to explore potential worthiness using sensory tricks in the management of CD.Methods We recruited 75 patients (23 male,52 female,male female ratio:1:2.26;aged 19-72 years,mean (42.45 ± 13.09) years) admitted to the Dystonia Clinic Center of the First Affiliated Hospital of Dalian Medical University from March 2016 to April 2013.Standardized questionnaire on sensory tricks was used to investigate the sensory tricks first recognized in 75 patients retrospectively.Median nerve somatosensory evoked potential via multipoint recording was measured in 43 CD patients who manifested as simple rotation of neck.Bilateral amplitudes of P22/N30 derived from F (3,4) were compared.Results Frequency of sensory tricks in CD was 85.53% (64/75) in our research.As disease progressed,the type of sensory tricks used by patients may transform from one to another or even disappear.No statistically significant differences were found in ages among patients using classic sensory tricks,forcible tricks and patients without sensory tricks,whereas the complexity of the disease showed increased tendency among the three groups.Bilateral P22/N30 amplitudes of patients with classic sensory tricks showed no significant differences.P22/N30 amplitudes contralateral to the head turn were significantly higher than ipsilateral in patients using forcible tricks ((3.16 ± 1.71) μV vs (2.47 ± 1.28) μV,t =2.243,P=0.038) and patients without sensory tricks ((3.62 ±1.58) μV vs (2.73 ±1.14) μV,t=2.893,P=0.023).Tsui scores among patients using classic sensory tricks (9.36 ± 3.52) and forcible tricks (12.67 ± 5.00) or patients without sensory tricks (15.18 ± 4.07) had statistically significant differences (classic sensory tricks vs forcible tricks,t =-3.020,P =0.004;classic sensory tricks vs patients without sensory tricks,t =-4.452,P =0.000).Conclusions Types of sensory tricks used by patients are associated with severity of the disease.Different types of sensory tricks may reflect status of adaptive compensatory mechanism of the cerebral cortex.
4.Analysis of correlation between hardiness and quality of life among patients with Parkinson disease
Tingting ZHANG ; Anchun YIN ; Tao LI ; Zhanhua LIANG
Chinese Journal of Behavioral Medicine and Brain Science 2018;27(4):348-351
Objective To investigate the current status of hardiness and quality of life (QoL) among patients with Parkinson disease (PD) and analyze the correlation between hardiness and QoL.Methods By convenience sampling,243 patients with PD were recruited from a tertiary hospital in Dalian during February 2017 and May 2017.General Scale,Hardiness Scale for Chinese Adults and Parkinson disease Questionnaire-39 item Version (PDQ39) were employed to measure hardiness and QoL among patients with PD.Results The hardiness score was (1.96±0.66) and the PDQ39 score was (35.46±20.38).The hardiness score was negatively correlated with PDQ39 score (r=-0.434,P<0.01).Conclusion Patients with PD have a low level of hardiness and QoL.The higher the level of hardiness,the better the QoL.