Objective:To systematically evaluate the association between the hepatic lipase ( LIPC) gene rs10468017 polymorphism and susceptibility to age-related macular degeneration (AMD). Methods:A systematic search was performed in both English databases (PubMed, Embase, EBSCO, Web of Knowledge and Cochrane library) and Chinese databases (CNKI, WanFang, VIP and CBM) from database establishment to December 31, 2019.Literature about LIPC rs10468017 polymorphism and AMD was searched.Odds ratios ( OR) and 95% confidence intervals ( CI) of allele mode (T and C), heterozygous model (TC and CC) and homozygous model (TT and CC) as well as the correlation between types of AMD and races were calculated using Stata 12.0 software. Results:Twenty-one studies were included in the Meta-analysis.There were 25 649 AMD patients and 26 294 normal controls.There was a significant correlation between LIPC rs10468017 polymorphism and risk of AMD in different genetic models (T vs. C: OR=0.83, 95% CI: 0.80-0.87; TC vs. CC: OR=0.82, 95% CI: 0.75-0.90; TT vs. CC: OR=0.65, 95% CI: 0.56-0.76). The subgroup analysis showed that the LIPC rs10468017 polymorphism was significantly associated with the risk of early AMD ( OR=0.87, 95% CI: 0.78-0.96), advanced AMD ( OR=0.83, 95% CI: 0.77-0.88), geographic atrophy ( OR=0.79, 95% CI: 0.72-0.87) and choroidal neovascularization ( OR=0.83, 95% CI: 0.78-0.89). Stratified analysis by ethnicity showed that there was a significant association between T allele and the decreased risk of AMD in the Caucasian population (early AMD: OR=0.77, 95% CI: 0.67-0.89; advanced AMD: OR=0.80, 95% CI: 0.75-0.87), but not in the Asian population (early AMD: OR=0.98, 95% CI: 0.85-1.13; advanced AMD: OR=0.93, 95% CI: 0.81-1.06). Conclusions:There is a significant association between T allele of LIPC rs10468017 polymorphism and the reduced risk of AMD, which exists in different subtypes of AMD with certain racial differences.

ObjectiveTo investigate the correlation of the serum levels of adiponectin， omentin， and visfatin with the severity of acute pancreatitis （AP）. MethodsBlood samples were collected from 35 healthy individuals in the control group and 70 patients with AP who were admitted to Chenggong Hospital Affiliated to Xiamen University from March 2022 to October 2023， and enzyme-linked immunosorbent assay was used to measure the serum levels of adiponectin， omentin， and visfatin in each group within 24 hours after admission. The analysis of variance was used for comparison of continuous between groups， and the LSD-t test was used for further comparison between two groups； the chi-square test was used for comparison of categorical data between groups. The Pearson correlation analysis was used to investigate the correlation between indicators， and the receiver operating characteristic （ROC） curve was used to investigate the value of the three indicators in predicting severe acute pancreatitis （SAP）. ResultsCompared with the control group， the AP group had significantly higher serum levels of omentin and visfatin （t=5.51 and 9.41， both P<0.01）， and the level of visfatin gradually increased with the aggravation of the disease （F=43.32， P<0.01）， while there was no significant change in omentin with the aggravation of the disease （F=0.47， P>0.05）. The AP group had a significantly lower level of adiponectin than the control group （t=-14.47， P <0.01）， and the level of adiponectin gradually decreased with the aggravation of the disease （F=35.61， P<0.01）. The serum level of visfatin was positively correlated with Acute Physiology and Chronic Health Evaluation II （APACHE-II） score （r=0.547， P<0.01）， and the level of adiponectin was negatively correlated with APACHE-II score （r=-0.520， P<0.01）， while there was no significant correlation between omentin APACHE-II score （r=0.007， P>0.05）. The three indicators had an area under the ROC curve （AUC） of 0.893， 0.570， and 0.829， respectively， in predicting SAP， and combined measurement of adiponectin and visfatin with an AUC of >0.7 showed an AUC of 0.953 in predicting SAP， with a sensitivity of 0.900 and a specificity of 0.933. ConclusionThe serum levels of adiponectin and visfatin are correlated with the severity of AP and have an important clinical significance in predicting SAP， and combined measurement of the two indicators has a higher value， while further studies are needed to investigate the correlation of omentin level with the severity of AP.

More and more evidence suggests that microRNA is widely involved in the regulation of cardiovascular function. Our preliminary experiment showed that miR-494-3p was increased in heart of diabetic rats, and miR-494-3p was reported to be related to metabolism such as obesity and exercise. Therefore, this study was aimed to explore the role of miR-494-3p in diabetic myocardial insulin sensitivity and the related mechanism. The diabetic rat model was induced by high fat diet (45 kcal% fat, 12 weeks) combined with streptozotocin (STZ, 30 mg/kg), and cardiac tissue RNA was extracted for qPCR. The results showed that the level of miR-494-3p was significantly up-regulated in the myocardium of diabetic rats compared with the control (P < 0.05). The level of miR-494-3p in H9c2 cells cultured in high glucose and high fat medium (HGHF) was significantly increased (P < 0.01) with the increase of sodium palmitate concentration, whereas down-regulation of miR-494-3p in HGHF treated cells led to an increase in insulin-stimulated glucose uptake (P < 0.01) and the ratio of p-Akt/Akt (P < 0.05). Over-expression of miR-494-3p in H9c2 cell line significantly inhibited insulin-stimulated glucose uptake and phosphorylation of Akt (P < 0.01). Bioinformatics combined with Western blotting experiments confirmed insulin receptor substrate 1 (IRS1) as a target molecule of miR-494-3p. These results suggest that miR-494-3p reduces insulin sensitivity in diabetic cardiomyocytes by down-regulating IRS1.
Animals ; Diabetes Mellitus, Experimental ; physiopathology ; Down-Regulation ; Insulin ; Insulin Receptor Substrate Proteins ; physiology ; Insulin Resistance ; MicroRNAs ; genetics ; Myocytes, Cardiac ; physiology ; Rats