Objective To discuss the training effect driven by clinical demand in a cross-sectional research, and provide evidence for specialized nursing training.Methods One month before the survey, 46 nurses received various forms of comprehensive training, including multimedia lectures, workshop, case analysis, group discussion, clinical demonstration teaching of ostomy wound, research operations training, risk assessment training of pressure ulcer and incontinence associated dermatitis, etc. Then theory examinations, picture analysis examinations and operation examinations were conducted before and after training based on the unified papers and operating criteria. Results After training, the score of theoretical examination, operating examination and picture analysis examination of 46 nurses were higher than that before the training (P<0.05). 89.1% of nurses thought that the training content was very good;93.5% of nurses were satisfied with the training form, and 95. 7% of nurses thought that this training was significant for clinical work. Conclusions The comprehensive training method based on clinical demand can effectively improve the professional theory and skills of nurses, thus it can be used as a effective training and assessment methods before the survey of pressure ulcer and incontinence dermatitis in comprehensive hospitals.