Objective: To investigate the effect of upper limb rehabilitation robot assisted training on the event-related potential P300, upper limb function and quality of life in stroke patients. Methods: A total of 90 stroke patients admitted to Yuyao People's Hospital from May 2017 to January 2018 were randomly divided into control group(45 cases) and observation group(45 cases) according to the digital table.The control group received conventional rehabilitation exercise, and the observation group was supplemented by Motorika ReoGo upper limb rehabilitation robot on the basis of the control group.The P300 latency, P300 amplitude, upper limb function and quality of life of the two groups were compared before and after treatment. Results: Before training, the latency of P300, amplitude of P300, upper limb function and quality of life score had no statistically significant differences between the two groups(all P>0.05). After training, the latency of P300, amplitude of P300 in the control group were (389.54±28.19)ms, (2.79±0.23)μV, respectively, which in the observation group were (356.25±24.54)ms, (3.06±0.19)μV, respectively.The latency of P300 in both two groups was significantly shorter than those before training(t=4.490, 12.342, all P<0.05), and the amplitude of P300 were significantly higher than those before training(t=-3.476, -10.061, all P<0.05), which of the observation group were significantly better than those of the control group(t=-5.975, 6.071, all P<0.05). After training, the Fugl-Meyer scale(FMA) and Barthel scale(MBI) scores of the control group were (35.62±9.72)points, (81.35±10.96)points, respectively, which of the observation group were (44.65±11.23)points, (89.63±11.59)points, respectively.The FMA and MBI scores in the two groups were significantly higher than those before training(t=-5.107, -8.595, -9.039, -11.417, all P<0.05), which of the observation group were significantly higher than those of the control group(t=4.078, 3.482, all P<0.05). Conclusion Motorika ReoGo assisted upper limb rehabilitation robot can significantly improve P300 latency, amplitude, upper limb function and quality of life in stroke patients.