Objective To investigate the relationship between the autoantibody detection and abortion of pregnant women.Meth-ods The dot immunogold method,indirect immunofluorescence and enzyme-linked immunosorbent assay(ELISA)were used to de-tect serum anti-double stranded DNA antibody (dsDNA),anti-smooth muscle antibody (SMA),antinuclear antibody (ANA),anti-endometrial antibody (EMAb),anti-cardiolipin antibody(ACA),anti-sperm antibody (ASAb),anti-ovarian antibody(AoAb).Re-sults Compared with healthy pregnant women,the values of EMAb,ACA,AoAb,ANA and SMA in abortion pregnant women were increased with statistical differences (P <0.05).Conclusion EMAb,ACA,AoAb,ANA and SMA have high correlation with abortion of pregnant women.Confirming the causes of unexplained abortion and prenatal screening are especially important for the pregnant women in the border area.

The multiple primary carcinoma (MPC) is defined as more than 2 or up carcinomas occured with in same or multiple organs in an individual.Along with the science development and advancement of human society,the life-span of human being has been prolonged,and more of MPC are identified.But the misdiagnosis of MPC as the metastases of primary tumors or missed diagnosis will mislead therapeutic regimens and exacerbate the diseases.Therefore,the accurate differentiation of MPC is becoming increasingly important.This article mainly reviews the epidemiology,diagnostic criteria,etiology,frequent sites of involvement,classification,and diagnostic protocols of MPC.

Objective:To construct an expression vector containing RNA interfering(RNAi) sequence targeting human vitamin D_3 receptor(VDR) protein gene,and to investigate its inhibitory effect on the expression and function of VDR in a human osteosarcoma cell line.Methods: Two small interfering RNA sequences targeting VDR were designed by online screening and were cloned into the expression vector(pSilencer-2.1-U6).Then the products,pSilencer-2.1-U6-VDR1 and pSilencer-2.1-U6-VDR2,were separately transfected into a human osteosarcoma cell line(HOS-8603) by liposome.Western blot was employed to determine their effect on VDR protein expression and 1,25(OH)_2D_3-induced phosphorylation of PKC.Cell counting and MTT were used to evaluate their influence on the inhibitory effects of 1,25(OH)_2D_3 on HOS-8603 cells proliferation.Results: Compared to HOS-8603 cells transfected with control vectors,those transfected with pSilencer2.1-U6-VDR1 or pSilencer2.1-U6-VDR2 had significant decreases in the expression of VDR protein, in the inhibitory effects of 1,25(OH)_2D_3 on the cell proliferation,and in quick phosphorylation of PKC induced by 1,25(OH)_2D_3.Conclusion: The vectors constructed in the present study(pSilencer2.1-U6-VDR1 and pSilencer2.1-U6-VDR2) can significantly suppress the VDR expression in HOS-8603 cells,and block some biological activities of 1,25(OH)_2D_3 mediated by VDR, which paves a way for further study on the biological functions of VDR and vitamin D_3.

Objective:To study the structure of teacher’s app raisal of pupil’s self-regulationMethod:Based on the open que stionnair es that teachers provided of their students’ characteristics, a self-regulation questionnaire of primary school students was developed 736 students were asse ssed by 56 teachersResults:Through exploratory factor analysis and confirmatory factor analysis, four factors, independence and conscientiousn ess, assault and resisting, friendship and obedience, irritability and activenes s, were obtained The ideal reliability and validity were gotConclusi on:In the view of teachers, the self-regulation structure of primary sch ool students includes four factors

Like other mammals,human has obvious circadian rhythm,and light is recognized as the principal circadian synchronizer.Rod/cone photoreceptors of the outer retina and the melanopsin-containing retinal ganglion cells(mcRGCs) of the inner retina mediate non-image forming visual responses including entrainment of the circadian clock to the ambient light,the pupillary light reflex (PLR),and light modulation of activity by means of a set of mcRGCs projecting to the suprachiasmatic nucleus (SCN)through retinohypothalamic tract (RHT).Glaucoma is a group of diseases which cause degenerative changes and loss of RGCs including mcRGCs,so the circadian rhythm of patients with glaucoma will be interrupted.The progress in the study on the effect of glaucoma and experimental hypertension on circadian rhythm was reviewed.

Glaucoma is a common eye disease which may cause irreversible blindness.This ocular degenerative disease affects retinal ganglion cells (RGCs) and eventually causes optic nerve dysfunction by way of axonal loss.Patients with glaucoma may show characteristic changes of optic disc,damages of visual field and impairment of vision.Detection and evaluation of the impairment of visual field in patients with glaucoma are essential for the diagnosis and estimation of progression of this disease.Numerous techniques have been developed to detect field progression,but no standard has been widely accepted.Assessment Methods including visual field indices,glaucoma hemifield test (GHT),Advanced Glaucoma Intervention Study (AGIS),Early Manifest Glaucoma Trial (EMGT),Collaborative Initial Glaucoma Treatment Study(CIGTS),as well as the strength and limitation of each method were reviewed in this article in order to find out suitable ways to evaluate visual field defects in patients with glaucoma in clinical works and researches.

Objective]To explore Professor YE Jin’s clinical experiences for dwarfism treatment based on spleen-insufficiency constitution related theory. [Methods]From the concept of dwarfism based on TCM, etiology and pathogenesis, key symptoms of spleen-insufficiency constitution and features medication to analyse the academic perspective and clinical experience of Professor YE Jin in the treatment of dwarfism, and list 1 proven case.[Results]Professor YE Jin thinks there’re 5 types constitutions of dwarfism, and attaches importance to adjust the unbalanced constitution of the patients. She thinks spleen-insufficiency constitution is basis of the other four types. She does well in enlivening and invigorating the spleen and activating spleen-energy.[Conclusion] Professor YE Jin has unique clinical experience for dwarfism treatment based on spleen-insufficiency constitution related theory, with significant effect.

New method of prostate cancer has grown more precise with the development of medical imaging.Multi-parametric MRI targeted biopsy and fusion biopsy reduce the cores,at the same time they have similar positive rates compared to systematic biopsy.The most used biopsy methods are still transrectal guided transrectal biopsy and transperineal biopsy in China.If we can predict biopsy result before the operation by physical and laboratory examination,it will help improve the detection rate and avoid repeating biopsies.And it has been indicated that age,tPSA,PSAD,fPSA/tPSA,PV,DRE,PHI and PCA3 are the predicting factors.Chinese Prostate Cancer Consortium Risk Calculator is fit for Chinses people.

BACKGROUND:Some Chinese herbal ingredients produce the analogical and pharmacological effects equivalent to growth factors, which can promote cel proliferation as wel as tissue and organ recovery and regeneration. Additional y, they possess low price and relative stable physicochemical property. Thus, the applications of traditional Chinese medicine in tissue engineering has attracted more attentions of researchers al over the world. OBJECTIVE:To review the application of the traditional Chinese medicine in stem cel proliferation and tissue engineering. METHODS:A computer-based retrieval of PubMed and CNKI databases was performed for articles concerning the application of traditional Chinese medicine in stem cel proliferation and tissue engineering published from 1978 to 2015. The keywords were“the traditional Chinese medicine, tissue engineering, application, stem cel , anti-inflammatory, cross linking, surface modification, osteoblast, blood vessel”in English and Chinese, respectively. RESULTS AND CONCLUSION:Natural medicine and the traditional Chinese medicine are expected to be applied in tissue engineering as a surrogate of growth factor and antibiotics, which exert anti-inflammatory, angiogenesis, surface modification, anticoagulation and cross-linking roles as wel as promote proliferation and differentiation of stem cel s in tissue engineering.

Epstein-Barr virus (EBV) is a DNA virus linked to a variety of human malignant tumors.In the 2008 WHO classification of lymphatic hematopoietic tissue tumor,EBV positive DLBCL of the elderly had been a new variant.The characteristic of this variant is different from other DLBCL subtypes.This paper summarized the etiology,pathogenesis,clinicopathologic features,immunophenotype,genetic changes,differential diagnosis and prognosis of EBV positive DLBCL of the elderly.