The nanoparticle-based electrochemical technology for early detection of cancer is an imjportant research topic in the area of biomedicine.This article introduces the concept of tumor marker and principle of electrochemical detection of the tumor marker.The applications of nanoparticles in electrochemical early detection of cancer are reviewed in detail.Finally,the prospected application of research is discussed.

[Objective]To evaluate outcome of the treatment of unstable distal radius fractures in the elderly patient using a volar T-shape plate.[Method]From January 2001 to April 2003,42 cases with unstable distal radius fractures older than 60 years(range 60～75 years;mean age 65.1 years) were treated with a T-shape plate fixation.The fractures were classified according to the AO classification of distal radius fracture: 4 A2,7 A3,5 B1,3 B3,9 C l,11 C2,3 C3.Artificial bone graft substitutes were used in 28 cases with severe cortex destruction and insufficient buttress.[Result]All patients were followed up(mean 18.2months,range 12～24months).Final volar tilt was averaged 3.66? and radial tilt 20.1?,and radial shortening averaged 1.59 mm in 6 cases.Step-off or gap of the articular surface was found in 6 cases(mean

0 05) after treatment.At the same time,the clinical symptoms were improved significantly.Conclusion ① Testing expression level of HLA DR is helpful to diagnose and monitor the state of RA.② The change of expression level of HLA DR conforms to that of rheumatic factor.③ Examination of the change of expression level of HLA DR can help to judge the state and prognosis of RA.

Objective To explore the clinical characteristics and the prognosis of benin convulsions as-sociated with mild gastroenteritis( BICE) in children. Methods To study clinical features of 248 children with BCIE,they followed up for 12 months,and their prognosis were observe. Results Age between 3 months and 3 years,twice or more seizures were occurred on 124 patients. 64 patients suffered the seizures more than 30 mi-nutes. Cranial CT/MRI showed no abnormality. Laboratory examinations were normal, including plasma glu-cose,serum electrolytes and cerebrospinal fluid. Followed up for 12 months,14 patients (5. 6%) suffered from recurrence of BICE,12 patients (4. 8%) suffered from simple febrile seizures,and 8 patients (3. 2%) devel-oped epilepsy. Conclusion The prognosis is good in majority of cases,but some cases may transform febrile seizure or epilepsy. It is more likely to transform epilepsy,when the children suffer from febrile seizure.

A size exclusion chromatography method ( SEC) was established for the screening of macromolecules in traditional Chinese medicine (TCM) injections. In the experiment, TCM was collected through 0. 45μm hybrid filter and separated by SEC with MAbPac SEC-1 column. Acetonitrile was added by post-column mode with a flow rate of 0. 2 mL/min to enhance the detection sensitivity, and 25 mmol/L ammonium formate was used as the mobile phase at flow rate of 0. 2 mL/min. The charged aerosol detection was carried out with Dextran and Tween as the reference standards. The molecular weight distribution of Dextran ranged from 2500 to 133800 had a good linear relationship with the retention time. No macromolecules were detected out in the selected traditional Chinese medicine injections except Kuhuang injections.

Objective Using 16S rRNA gene sequencing as the gold standard method,to compare the performance of two matrix-assisted laser desorption ionization time of flight mass spectrometry system (MALDI Biotyper and VITEK MS) for identifying clinical isolates of Streptococcus spp.Methods One hundred and sixty two clinical Streptococcus isolates were collected at the Second Affiliated Hospital of Zhejiang University,from April to June,2014,and confirmed by 16S rRNA gene sequencing analysis.MALDI Biotyper and VITEK MS mass spectrometry system were used for identification and further evaluated by performance respectively.Results Of all the isolates tested,155 (155/162,95.68％) Streptococcus isolates were accurately identified to species level by MALDI Biotyper.Besides,MALDI Biotyper identified three Streptococcus mitis group as S.pneumoniae and one S.parasanguinis as S.australis.Another three S.pneumonia isolates were not identified accurately (values ＜ 1.7).Although 156 (156/162,96.30％) isolates were accurately identified to species level (including subspecies) by VITEK MS system,two S.pneumoniae as S.mitis/S.oralis and one S.euinus as S.infantarius ssp.infantarius were misidentified.The two systems showed a 100％ (51/51) accuracy in identifying all S.pyogenes and S.agalactiae isolates,and an accuracy higher than 85％ for S.pneumoniae.Conclusions Both systems showed potent identification ability for Streptococcus spp.,VITEK MS system showed more clinical significance in accurately identifying some subspecies.Mass spectrometry system can be used as a rapid identification method for Streptococcs spp.in clinical practice.

Angiotensin II ; metabolism ; Cisplatin ; adverse effects ; Humans ; Kidney ; drug effects ; Kidney Diseases ; chemically induced ; metabolism

Purpose:To investigate method of early detection of breast cancer with microcalcification observed by mammograph but without palpable mass clinlcally.Methods:Stereotatic core needle biopsy (SCNB) were performed in 35 patients with calcification observed by mammography,and 29 people received stereotatic needle localized breast biopsies (NLBB).All tissues were routinely processed.Microscopic analysis of calcification and morphologic analysis of calcifica- tion were done,as well as histologic diagnosis.Results:Among the 35 specimens of SCNB,microscopic calcification,in- traductal carcinoma and invasive ductal carcinoma were detected in 24,8,and 4 respectively.Calcification was identified in 25 of the 29 cases of NLBB.Five cases of intraductal carcinoma,six cases of invasive ductal carcinoma as well as one case of invasive lobular carcinoma were diagnosed in these 29 patients.Conclusions:With close cooperation among pathol- ogists,surgeons and radiologists,the application of SCNB and NLBB may benefit the early detection of breast cancer with microcalcification observed by mammograph but without mass being palpable clinically and finaly improve the survival of breast cancer patients.

Objective To explore high stage affective theory of mind(ToM) of alexithymics.Methods 24 alexithymics (study group,TAS-20 ≥ 61) and 26 non-alexithymics (control group,TAS-20 ≤ 51) were screened from college students according to the sore of TAS-20.The scores of reading the mind in the eyes test(RMET),fixation numbers and fixation duration in area of interest (AOI) were recorded by HiSpeed Eye Tracker.The data were analyzed by Mann-Whitney U test and Chi-square test.Results There was no differences in scores of RMET between control group and study group (M(Q):23.50(5.00) vs 24.00 (5.00),Z=-1.044,P＞0.05).Fixation numbers and fixation duration in study group were higher than those in control group (fixation number:616.00(412.80) vs 579.00(371.30),Z=-2.010,P＜0.05;fixation duration:187363.35(106339.28)msvs 139087.55(67335.58)ms,Z=-2.632,P＜0.01).Conclusion Alexithymics are impaired in high stage affective ToM.