People- oriented is the purpose of modem medicine pattern.To realize the purpose,the medical worker must improve humanity accomplishment all round,morals - oriented,and serve for common people with humanity character of the nobleness and perfect medical treatment technology.

Female ; Humans ; Incontinentia Pigmenti ; diagnosis ; pathology ; therapy ; Infant, Newborn

Objective To investigate the expression of PTPN13 in human gastric cancer and gastric cancer SGC-7901celllineanditsassociationwithproliferationandinvasion.Methods 106casesgastriccancertissuesamples and matched normal peritumorial tissues were collected .SGC-7901 cells were cultured and divided into two groups including pcDNA3.1-PTPN13 transfection group and without transfection group .Immunohistochemical technique was used to detecte protein expression of PTPN 13.The association of PTPN13 expression with tumor location , tumor size , depth of invasion and tumor metastasis were analyzed .The survival rate of patients with different PT-PN13 expression was calculated by Kaplan-Meier curves.CCK-8 assay was used to estimate the proliferation chan-ges.Using Trans-well assay analyzed the invasion of SGC-7901 cells.Moreover, Western blot was performed to de-tect the markers of EMT including E-cadherin, Snail and MMP9.Results Immunohistochemistry showed the expression rate of PTPN13 in gastric cancer tissues was lower than normal tissue (31 %vs 83%, P<0.05).The expression of PTPN13 in patients was related to with different tumor size , depth of invasion and tumor metastasis (P<0.05).The 2-year survival rate of patients with negative PTPN13 expression were lower.Overexpression reduced both proliferation and invasion in SGC-7901 cells.Up-regulated PTPN13 may increase E-cadherin level but decreases the level of Snail and MMP9 .Conclusions PTPN13 plays a role in gastric cancer tissue and cells as a tumor suppressor.Lower PTPN13 may predict a poor prognosis.PTPN13 may be used as therapy target in gastric cancer.

Objective To evaluate the epidemic status of main non-communicable chronic diseases among the residents of Jingan district in Shanghai.Methods Multistage cluster sampling was adopted.960 residents were investigated with questionnaire and physical examination.Results The prevalence rates of Hypertension,diabetes mellitus,Cerebral vessels and Heart-blood were 25.6%,5.8%,3.1%and 5.7%,respectively.The multivarate logistic regression analysis showed that the related risk factors to four non-communicable chronic diseases included family history,the OR(95%CI)1.6(1.2～2.3),6.9 (3.5～13.6),4.5(2.0～10.0),2.2(1.2～4.0)separately.Conclusion Hypertension,diabetes are the main non-communicable disease hazarding the public health.The government and professional institutes should raise the attention on this serious problem and finance more as well as supplying policy support.

Seven sinapine analogs (6a-6g) were synthesized using cinnamon acid or benzoic acid and their derivatives as starting materials, which obtained from substituted benzaldehyde and malonate. The structures of target compounds were characterized by IR, 1H NMR and elemental analysis. The effects of compounds 6a-6g on the smooth muscle of intestine isolated from rabbit were studied, and the experimental results showed that compounds 6a, 6d and 6g had diastolic action, while 6f had contractile action.

OBJECTIVE:To provide a new management mode for the rational use and standardized management of narcotics in hospital.METHODS:The fingerprint management system of narcotics was build up and applied.RESULTS:The fingerprint management system of narcotics could accurately help identify patients' identity and provide effective supervision for using narcotics.CONCLUSION:The fingerprint management system of narcotics can replace the current management mode for it makes up the shortage of the traditional management system.

Objective To establish a method for the determination of Puerarin in Huanglian Jiangtang Tablets. Methods The sample was extracted with 30 %ethanol. The chromatographic conditions were as follows:Diamonsil C18 chromatographic column(250 mm?4.6 mm,5?m)with a mobile phase of acetonitrile-0.5 %glacial acetic acid,the detection wavelength being at 250 nm and the flow rate being 1.0 mL?min-1. Results A linearity was obtained from 0.338 ?g to 2.336 ?g of Puerarin in Huanglian Jiangtang Tablets with a good correlation (r=0.999997,n=7).The average recovery was 100.2% and RSD=1.80%(n=6). Conclusion This method for determination of Puerarin in Huanglian Jiangtang Tablets is easy,sensitive,specific and accurate.

Objective To establish a method for the determination of 5-O-methylvisamminol in Shufeng oral liquid.Methods The sample was extracted with methyl and HPLC method was used.The chromatographic conditions were as follows: Alltima-C18 column(150 mm ? 4.6 mm,5 ?m)with a mobile phase of methanol-water(41∶59),the detection wavelength at 293 nm and the flow rate being 1.0mL?min-1.Results A linearity was obtained from 0.12 ?g to 0.84 ?g of 5-O-Methyvisammioside in Shufeng oral liquid with a good correlation(r = 0.99998,n = 7).The average recovery was 99.8 % and RSD = 2.05 %(n = 5).Conclusion This method for determination of 5-O-Methyvisammioside in Shufeng oral liquid is simple,sensitive,specific and accurate.

Objective To assess the value of video-assisted thoracoscopic mini-invasive lobectomy in the treatment of pulmonary benign lesions or malignancies. Methods A retrospectively analysis on clinical data of 35 cases of video-assisted thoracoscopic mini-invasive lobectomy, which were performed from February 2002 to June 2006, was made. The pathological diagnosis included 31 cases of peripheral lung cancer (T_1N_0-1M_0), 3 cases of bronchiectasis, and 1 case of atelectasis combined with pulmonary abscess. The procedures included 30 cases of anatomical lobectomy and 5 cases of non-anatomical lobectomy. Results No conversion to open surgery was required. The operation time was 85~210 min (115?35 min) and the intraoperative blood loss was 150~450 ml (210?55 ml). Postoperatively, acute respiratory failure was encountered in 2 cases, and mechanical ventilation was given for 3 and 5 days, respectively. Acute myocardial infarction occurred in 1 case, which was cured by early-stage thrombolysis. No postoperative complications were seen in the remaining 32 cases. The closed chest drainage was maintained for 2~10 days (mean, 5.4 days). In 3 cases of pulmonary benign lesions, follow-up checkups for 41, 33, and 13 months, respectively, found no long-term complications. In 25 cases of lung cancer, follow-up observations were made for 3~53 months (mean, 15.2 months), including 3~12 month in 6 cases, 12~24 months in 9 cases, 24~36 months in 7 cases, and 36~53 months in 3 cases. There were 4 cases of death due to cancer recurrence or metastasis (16%), 2 cases of with-tumor survival (8%), and 19 cases of disease-free survival (76%), respectively. Conclusions Video-assisted thoracoscopic mini-invasive lobectomy is a feasible and safe procedure for peripheral lung cancer and benign lung diseases.

Objective To study the feasibility of preoperative acute hypervolemic hemodilution for decreasing perioperative blood use. Methods Thirty two patients(ASAI-II) undergoing spinal surgery were randomized into AHH group and control group. After anesthesia 6% HES(200/0.5) was given through internal jugular vein for AHH. The blood volume was increased by about 25%. During the operation, blood loss was replaced by HES in equal volume. Blood was transfused when the Hct dropped below 25%. The HR, MAP, CVP, SPO2, Hb, Hct., Plt, FIB, APTT, PT, amount of blood loss, blood transfused and urine output, volume of drainage 24h after operation and VAS score (0 means no effect, 10 means maximal effect) were observed before AHH, at the end of AHH, at the end of operation, and 24h after the operation. Blood volume expansion was calculated. Results (1) HR increased significantly in both groups(P