Cathepsin S is one of the cathepsins that distributes generally at lysosomes of histiocyte in humans body.It has variety of actions when it is unbalancing,including promote inflammation,atherosclerosis,diabetes and obesity,etc.Studies in recent years have demonstrated that there are certain correlations of adiponectin with vascular risk factor and cerebrovascular disease.

Objective To explore the expressions and significances of apoptotic modulating genes Fas and Bcl-2 mRNA in peripheral blood monocytes(PBMC)of patients with myasthenia gravis (MG). Methods The levels of Fas and Bcl-2 mRNA in PBMC were detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) in 44 patients with MG as well as 30 healthy donors served as a normal control (NC).Results (1) The level of Fas mRNA in MG group was no significantly difference compared with NC group. The level of Bcl-2 mRNA in MG group was higher than that in NC group(P

Objective To determine whether rapid and delayed tolerance to focal cerebral ischemia can be induced by pretreatment with single electroacupuncture (EA) at the Baihui acupoint in rats. Methods Forty-eight healthy male SD rats weighing 280-320 g were randomly divided into 6 equal groups with 8 animals in each group: (1) control group received no pretreatment before focal cerebral ischemia; (2) electroacupuncture group was further divided into 5 groups in which EA was performed at Baihui acupoint and electric stimulation was maintained for30min0.5 h(EAl),1 h(EA2),2 h(EA3),3 h(EA4) or 24 h(EA5) before focal cerebral ischemia. The animals were anesthetized with isoflurane and spontaneous respiration was maintained. Middle cerebral artery occlusion (MCAO) was performed using a nylon thread with a rounded tip inserted into internal carotid artery and thread cranially until resistance was felt. MCAO was maintained for 2 h, then the nylon thread was withdrawn to allow reperfusion . After MCAO the animals were observed for 24 h for neurological deficits which were assessed by a 0-5 grade scoring system (0 = no deficit, 5 = death) . The animals were then sacrificed and brain was immediately removed for estimation for the size of cerebral infarction. Results There were significantly less neurological deficits and the size of infarction was smaller in group EA3 and EA5 than those in control group. There were no significant differences in neurological deficits and size of infarction between group EA1,EA2,EA4 and control group. Conclusion Pretreatment with single electroacupuncture stimulation for 30 min at Baihui acupoint can induce rapid and delayed tolerance to focal cerebral ischemia. Rapid tolerance develops 2 h after pretreatment while delayed tolerance develops 24 h after pretreatment.

Objective To evaluate the effective treatment for and prevention of complications. Methods The diagnosis and treatment of 32 patients with acute arterial ischemia of the lower extremity were analyzed (retrospectively). 4 cases were treated with thrombolytic therapy, 28 cases underwent emergency operation, of which 3 cases underwent amputation after embolectomy, and 25 cases were treated by arteriotomy of femoral artery and embolectomy with Fogarty catheter(7 cases underwent concomitant arterioplasty).Results One (patient) in the nonoperative group died; but there was no mortality in the operated group. Limb amputation was done in 3 cases, and limbs were saved in 28 cases. Conclusions For acute arterial ischemia of the lower (extremity), emergency arteriotomy of femoral artery plus embolectomy is the treatment of choice. This (therapeutic) approach can lower mortality and limb amputation rate.

Objective To detect COX-2 mRNA in cervical cancer and normal cervix, and explore the pathogenesis of adenocarcinoma of the uterine cervix. Methods COX-2 expression was detected by RT-PCR to explore the relationship between COX-2 and the adenocarcinogenon of cervical cancer in 36 cases of adenocarcinoma of the uterine cervix and 15 cases normal cervical tissue. Results The positive rate of COX-2 mRNA in adenocarcinoma of the uterine cervix tissues (55.6%) was higher than that of normal tissues ( 20. 0% ), the difference was significant ( P ＜ 0. 05). The expression of COX-2 mRNA was associated with stages and clinical grading of tumor ( P ＜ 0. 05), but no significant difference was found between stages and clinical grading of uterine cervix adenocarcinoma( P ＞ 0. 05). Conclusion The hyper expression of COX-2 mRNA in uterine cervix adenocarcinoma took part in the carcinogenesis of tumor.

MicroRNA are endogenous non-coding small RNA composed of approximately 18 - 23 nucleotides, mainly participating in post-transcription gene regulation. More and more data show that miR-21 is over-expressed in many human cancer cells and it was involved in cell differentiation, proliferation and apoptosis, closely related to tumorigenesis and the development of carcinoma as oncogene. Here, we will make a review about miR-21' s target gene, biosynthesis and regulation and clinical significance in order to provide a valued data for the future study and clinical application.

Objective To summarize the nursing methods and effect of artificial femoral head replacement in treatment of osteoporotic interttochanteric fracture in elderly patients. Methods 55 patients with esteoporotic intertrochanteric fracture were selected in our hospital from February 2007 to February 2011.All patients were cared by targeted preoperative and postoperative nursing,helping them conducting functional training in time,giving discharge counseling and follow-up.The hip functional recovery of patients was evaluated by using the Harris evaluation standard,and the excellent rate of surgery was also evaluated. Results After the targeted care,55 patients went through the perioperative period safely,the average length of stay was ( 14.0 ± 3.1 )days,a total of 5 cases (9.09％)patiants suffered varying degrees of complications which were improved after symptomatic treatment.Patients were followed up for 3 to 36 months,only 2 patients died of cerebral vascular accident,all patients gained good recovery of hip function.Harris score was (84.45 ± 9.38),the excellent rate of surgery reached 78.18％. Conclusions Atificial femoral head replacement proves significant effect in treatment of osteoporotic intertrochanteric fractures in elderly patients,but with the accompanying surgical compliuations,special care and timely functional exercise methods should be paid attention to.

OBJECTIVE: To discuss the influences of the concentrations of sodium cholate, cholesterol and drug, preparation temperature, ultrasonic time and power on the particle size and encapsulation efficiency of Flos Magnoliae volatile oil nano-liposome through film-ultrasonic wave technique together with membrane filter method. METHODS: The nano-liposomes were prepared by film-ultrasonic wave technique together with membrane filter method. The drug content in liposomes was determined by gas chromatography and the particle size was determined by laser light scattering granularity equipment. RESULTS: By film-ultrasonic wave technique together with membrane filter method, the liposomes in the range of 30-40 nm were prepared. The prepared nano-liposomes demonstrated good decentralization and homogeneity, and the encapsulation efficiency was (91.7+/-3)%. CONCLUSION: Film-ultrasonic wave together with membrane filter method is a simple method to fabricate nano-magnoliae liposome.

While the field of ATP synthase research has a long history filled with landmark discoveries, recent structural works provide us with important insights into the mechanisms that links the proton movement with the rotation of the Fo motor. Here, we propose a mechanism of unidirectional rotation of the Fo complex, which is in agreement with these new structural insights as well as our more general ΔΨ-driving hypothesis of membrane proteins: A proton path in the rotor-stator interface is formed dynamically in concert with the rotation of the Fo rotor. The trajectory of the proton viewed in the reference system of the rotor (R-path) must lag behind that of the stator (S-path). The proton moves from a higher energy site to a lower site following both trajectories simultaneously. The two trajectories meet each other at the transient proton-binding site, resulting in a relative rotation between the rotor and stator. The kinetic energy of protons gained from ΔΨ is transferred to the c-ring as the protons are captured sequentially by the binding sites along the proton path, thus driving the unidirectional rotation of the c-ring. Our ΔΨ-driving hypothesis on Fo motor is an attempt to unveil the robust mechanism of energy conversion in the highly conserved, ubiquitously expressed rotary ATP synthases.
Membrane Potentials ; physiology ; Membrane Proteins ; chemistry ; metabolism ; Mitochondrial Proton-Translocating ATPases ; chemistry ; metabolism ; Protein Conformation