With the improvement of cataract operation, the cataract surgery has become increasingly perfect. The cataract patients show greater expectation for the result of cataract operation. As a result, refractive cataract surgery has become the main trend. Detailed investigations of corneal diseases, lens density, corneal topography, preferable intraocular lens ( IOL ) choice, and IOL power calculation can help us get a better knowledge of preoperative conditions on patients, which can be conducted with pentacam. So we can have a better forecast of post - operative outcome and improve the quality of vision for cataract patients after surgery.

Objective:To investigate the protective effects of matrine(MT) preconditioning against hepatic ischemia-reperfusion injury in rats. Methods:Fifty male SD rats were randomly allocated into 5 groups:sham operation(SO) group,ischemia-reperfusion (NS) group and MT preconditioning groups (of 3.75,7.5,15 mg/kg). Rats of NS and MT groups were injected normal saline or MT,respectively; and 15 min later,the pedicales of the left and median liver lobes were clampped for 45 minutes,and reperfused for 40 min. The portal trial were only separated but not obstructed in SO rats. After reperfusion,the serum activity of ALT,AST and LDH was determined,the activity of SOD and content of MDA in liver tissue were examined,and histopathological changes of liver were observed. Results:Compared with those of NS group,the serum levels of ALT,AST,LDH,and the content of MDA in liver tissue of MT groups were lower,and the activity of SOD was higher(P

BACKGROUND:To improve the survival rate of transplanted tissue, most scholars focus on cel therapy, particularly cel-assisted fat grafting.
OBJECTIVE:To analyze the effect of recombinant adenovirus-mediated hypoxia inducible factor 1alpha (HIF1α) mutant on survival rate of transplanted fat particles through transfection of adipose-derived mesenchymal stem cels.
METHODS:Recombinant adenovirus-mediatedtriple-mutantHIF1αwas inserted into an adenovirus pAdEasy-1 system, folowed by viral packaging and titer determination. Human adipose-derived mesenchymal stem cels were cultured, passaged and identified, and subsequently transfected with three kinds of viruses and blank vector (experimental group with transfection of the triple mutantof HIF1α;positive control group; negative control group; blank control group). Transfection efficiency was determined using enhanced green fluorescent protein labeling. Additionaly, MTT assay was used to detect cel proliferation.
RESULTS AND CONCLUSION:The recombinant adenovirus was successfuly constructed and packaged in line with transfection requirements. Moreover, adipose-derived mesenchymal stem cels were successfuly identified by adipogenic, osteogenic and chondrogenic induction and could be used asseed cels for subsequent experiments. RT-PCR results showed that HIF1α mRNA expression in the experimental group and positive control group was significantly higher than that in the other two groups (P< 0.05). Western blot analysis showed that the relative absorbance value in the experimental group was significantly higher than that in the other three groups (P< 0.05). A significant increase in the cel proliferation was found in the experimental group, significantly different from the other three groups (P<0.05). Therefore, our findings indicate that transfection of adenovirus-mediated triple-mutant HIF1α not only can sustain the expression of target protein in transfected adipose-derived mesenchymal stem cels under normoxic conditions, but also can promote the proliferation of transfected adipose-derived mesenchymal stem cels.

Objective To evaluate the clinical efficacy of facial acupoint catgut embedding therapy in the treatment of skin aging.Methods The sixty patients with facial skin aging were randomly divided into catgut embedding group and sham embedding group,30 cases each.Catgut embedding group received catgut embedding therapy; the control group also received acupuncture but not the filling line.Two groups were operated 2 times,once every 21 days,a total of 42 days.The facial skin aging were evaluated and analyzed using VISIA and CK complexion analysis system.Results In catgut embedding group,wrinkles,pores,texture,moisture and elasticity were improved signficantly (P＜0.05).Ultraviolet,pigmentation,telangiectasia and melasma were not obviously improved.There was no significant change in the sham embedding group.Conclusions Facial acupoint catgut embedding therapy is an effective method for the treatment of skin aging with no significant side effects.

Finite element analysis (FEA) is broadly used in biomechanics.Being widely used in clinical studies on cervical spine injury,cervical degeneration and stimulating a variety of cervical spine surgeries,cervical finite element model is becoming more and more accurate in recent years.This paper aims to review the development of cervical finite element model,to introduce the application progress of the modeling and analysis in cervical spine injury,cervical disc arthroplasty,cervical interbody fusion,cervical degeneration and cervical instability,and to prospect the foreground of cervical finite element model in future.

Objective:To observed the longitudinal changes of the facial growth in vertical dimension in the Chinese adolescents with normal occlusion from 13 to 15 years old.Methods:The subjects were from Beijing area and consisted of 9 males and 14 females aged 12.5-13.5 years with Class I skeletal and dental pattern and without previous orthodontic treatment. Serial 2-year- annual cephalometric radiographs were taken and digitalized. Descriptive statistics for the absolute and incremental changes of the skeletal cephalometric measurements were conducted.Results:Significant gender differences in the changes of facial growth in vertical dimension from 13 to 15 years old were observed. Most of the skeletal measurements in males showed greater value than those in females. The number of the measurements which showed gender difference increased with age. The annual incremental changes of TAFH, TPFH, LAFH, U6-PP in males and those of TPFH and RH in females from 13 to 14 years old were greater than those from 14 to 15 years, However, the average increment of L6-MP in males and that of UI-PP, LI-MP, U6-PP and OB in females from 13 to 14 years old were smaller than those from 14 to 15 years old. There was no significant gender difference in TAFH/TPFH from 13 to 15 years of age. Gender difference from 14 to 15 years old was found in the growth of dental-alveolar height. The main dento-alveolar height changes happened in lower incisors and lower molars in males and in the region of upper molars and upper incisor in females.Conclusion:The skeletal facial growth in vertical dimension from 13 to 14 year old falls into the accelerating period. After 14 years old, the growth velocity is slowed down. There are significant gender differences in the skeletal changes of facial growth in vertical dimension from 13 to 15 years old. From 14 to 15 years old, the gender differences are mainly found in the growth of the dento-alveolar height.

To observe the perioperative changes of erythrocyte (RBC) phosphofructokinase (PFK) activity and content of ATP and Mg~(2+) in patients undergoing open heart surgery. Method: Fifteen patients undergoing open heart surgery were admitted to this study. Arterial blood samples were collected for the measurements of PFK aczivity, contents of ATP and Mg~(2+) in RBC at 9 points: before anesthesia induction, spiliting sternum, the beginning of CPB.5 minutes after aorta occlusion, at the end of CPB, at the end of operation.on the lst,2nd and 3rd postoperative day. Result: Erythrocyte PFK activity had no marked change during anesthesia, but was decreased significantly on the 1st postoperative day compared with beseline (P

Objective To investigate the protection of panax notoginseng saponins(PNS) against ischemia reperfusion injury by liver graft in rats.Method The experimental rat model of orthotopic liver transplantation was set up according to Kamada's technique and the rats were randomly divided into three groups: normal saline control group(group N),PNS preconditioning group(group P) and sham-operated groups(group S).The expression levels of NF-?B and intercellular adhesion molecule-1(ICAM-1) were detected by immunohistochemistry and the filtration of polymorphonuclear neutrophils(PMNs) was measured with myeloperoxidase(MPO) enzyme method in 2h,6h and 24h after ischemia reperfusion respectively.The activities of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) in the serum were also detected.Result The activation of NF-?B in group N increased significantly at 2h after reperfusion and then decreased.The expression level of ICAM-1 increased significantly at 2h after reperfusion,and reached its peak level at 6h in group N.The expression level of ICAM-1 was significantly lower in group P than that in group N(P

Objective To evaluate the features of bacterial spectrum and drug resistance in diabetic ulcer.Methods Excretion specimen of diabetic ulcer from 130 patients admitted into our hospital from Mar.2010 to Sep.2011 were collected for bacterial culture and drug sensitivity test.28 antibiotics were chosen from 35commonly used antibiotics to test antibiotic sensitivity to decide whether the antibiotic was sensitive (S),intermediate sensitive (I),or resistant (R).Results 19 strains were isolated from 130 cases.Staphylococcus aureus with 14.62 ％ (19 cases)rated the first,followed by escherichia coli with 13.85％ (18 cases),proteus mirabilis with 13.08％ (17 cases),staphylococcus epidermidis with 12.31％ (16 cases),enterococcus faecalis with 12.31％ (16 cases),acinetobacter baumannii with 10.77％ (14 cases).Antibiotics with high sensitivity to Gram-positive bacteria were tigecycline and vancomycin while with high sensitivity to Gram-negative bacteria were imipenem and amikacin.Conclusions Bacteria of diabetic ulcer are commonly resistant to antibiotics.It is of great importance for clinical rational drug use to know the distribution and resistance of commonly encountered bacteria.

Objective To observe the findings of 1H magnetic resonance spectroscopy ( 1H MRS) and explore its value for diagnosis of radiation induced-temporal lobe necrosis in nasopharyngeal carcinoma (NPC). Methods Thirteen patients with definite clinical diagnosis of radiation induced temporal lobe necrosis in NPC underwent 1H MRS. All 1H MRS examinations were performed on a Singa Horizont 1.5 T whole body MR system with point resolved spectroscopy (PRESS) and chemical-shift selective saturation (CHESS) (TR=2000?ms,TE=144 ?ms). The voxel (2?cm ? 2?cm ? 2?cm) was positioned in both of the lesion and the contralateral side of the temporal lobe as the control (except for bilateral lesions). 2THZ]Results The interval between radiation and the examination ranged from 32 to 172 months ( median, 42 months). Eighteen lesions were observed in 13 patients (5 patients with bilateral lesions). 1H MRS was performed on 17 lesions and 8 control temporal lobes. The mean values of NAA/ Cr in the lesions and the control temporal lobes were 1.33?0.28、1.78?0.34 respectively(t=3.22, P=0.005). Three lesions showed NAA、Cho and Cr peak absent (2 lesions with Lip peak ). Among 8 patients with the control temporal lobes 1H MRS examined, Cho/Cr decreased in 6 patients (75%). Four lesions showed Lip , Lac and MI, respectively. Conclusions The radiation-induced temporal lobe necrosis shows abnormal features on 1H MRS. Significant decrease of NAA/ Cr as well as Cho/Cr are observed in most patients. 1H MRS may play a role in the diagnosis of radiation induced temporal lobe necrosis.