OBJECTIVE: To investigate the antagonistic effect of Salvia miltiorrhiza on cyclosporine A (CsA)-induced chronic nephrotoxicity. METHODS: 54 patients underwent renal transplantation were divided into 3 groups according to dosage of salvia miltiorrhiza: CsA+high-dose of salvia miltiorrhiza group, CsA+loe-dose of salvia miltiorrhiza group (trial group) and CsA control group (control group). Plasma concentration of CsA, serum creatinine (Scr), blood urea nitrogen (Bun), 24 h urines and 24 h urinary protein were recorded. The parameters of morphology and hemodynamics were compared among 3 groups. The differences of index among 3 groups were analyzed statistically. RESULTS: The level of Scr, Bun and 24 h urinary protein in CsA+high-dose of salvia miltiorrhiza group were decreased significantly while 24 h urines was increased, compared with other 2 groups. There was significant difference among 3 groups (P0.05). The intrarenal blood flow and renal arterial resistance index were increased significantly in CsA+high-dose of salvia miltiorrhiza group. CONCLUSION: High-dose of salvia miltiorrhiza combined with CsA can reduce the CsA-induced nephrotoxicity perfently.

As the dressings currently used in clinic settings unflat shape in general, they can't be fitted completely on occiput, heel, elbow, knee and other body parts unflat. This paper developed one kind of foam dressing of special shape based on local anatomy. The foam dressing is waterproof and air permeable, it can cover the wound closely enough to prevent bacteria from invasion and infection. With a saturated absorption ratio of 1: 8 or higher, it can keep the wound clean and moisture by absorbing large amounts of wound inflammatory secretions and is almost completely permeable to oxygen and carbon dioxide. Assuring safety and effect meanwhile, it has better outcomes than common dressings in the same application settings.
Bandages ; Wound Healing

Adolescent ; Female ; Humans ; Leukemia ; etiology ; Leukemia, Promyelocytic, Acute ; complications ; Male ; Young Adult

Child ; Hematology ; history ; History, 20th Century ; History, 21st Century ; Humans ; Pediatrics ; history ; Societies, Medical ; history

Objective: To research the application of perspective imaging of intense laser proton beam in tumor cells, experi-ments are carried out on an ultra-intense picosecond pulsed laser facility, and the results of perspective imaging on biological samples by high energy proton beam, which is excitated among the interaction of picosecond laser with solid target, is showed. Methods: The laser energy is 12 J, the pulse width of laser is 1.5 ps, the laser focal spot diameter is 15 μm, the coefficientf or the off-axis paraboloid lens is 1.5. The target used in the experiment is made by coating a CH film with 1 μm on the back of the 15 pan-thick Cu. The distance between the proton source (laser spot) and the ant sample is 7 mm (i.e. the object distance), the distance between the sample and the RC film is 63 mm (i.e. the image distance). The detector is a stack of RCF or CR-39 films. Results: The ant is taken as the sample, a high energy proton beam, which is excitated as above on the rear surface of target, makes a 2-dimensional perspective imaging of an ant with high contrast, nanometer spatial resolution and density reso-lution. Conclusions: The result provided possibility of the application of perspective imaging of intense laser proton beam in tumor cells.

Objective To study the effect of cold and heat treatment on metastasis and the peripheral blood immune response in patients with breast cancer. Methods A total of 124 breast cancer patients were randomly di-vided into a hot and cold therapy group and a control group, each of 62. Both groups were given conventional surgical resections, and the hot and cold therapy group was given cooling and heating physical therapy for one month before the operation. The efficacy of the treatment and the patients′ spiritual state were compared after the treatment. The effect on lung metastases was evaluated using tomographic images of the chest, and the changes in immune cell levels in the peripheral blood were detected using flow cytometry. Results After the treatment, the remission rate (54.8%), spiritual life ratings on the Karnofsky scale and the area of lung cancer nodule transfer were significantly better in the hot and cold therapy group than in the control group. Twelve weeks after the treatment, the percentage of MDSCs in the control group patients was 72.5%, significantly higher than the 5.3% of the therapy group. The CD4+and CD8+ T cells were 2.2% and 1.8%, significantly lower than the 26.7% and 13.9% of the control group. Con-clusion Cold and hot therapy before a mastectomy can effectively inhibit the metastasis of breast cancer and promote patients′physical rehabilitation and spiritual life. It may regulate the microenvironment of their immunosuppression and stimulate antitumor immunity.

Objective To understand the working stress source of nurse anesthetists and its coping measures. Methods The semi-structured in-depth interview was conducted in 11 nurse anesthetists from 4 hospitals. The collected data were analyzed and concluded by using Colaizzi’s seven step method of phenomenological analysis. Results Four themes were extracted and a series of sub-themes, including work environment, work characteristics and nature, confusion of career prospects and coping styles. Conclusions Managers should pay close attention to anesthesia care. The laws and policy of the qualifications standards for nurse anesthetists should be made, which can reduce the stress of nurse anesthetists including healthy and harmonious working environment, a professional training system of nurse anesthetists and career planning. So that we can improve the professionalism of nurse anesthetists.

BACKGROUND:The Notch signaling pathway is evolutionarily highly conserved and mediated by cel-cel interactions, which is widely involved in cel differentiation, proliferation and apoptosis. This critical transduction system participates in coordinating various functions of vascular endothelial cels, thus playing a vital role in the formation and remodeling of vascular network. OBJECTIVE: To elucidate the components of Notch signaling pathway and its impact on angiogenesis. METHODS: A computer-based online search of PubMed and CBM databases was performed. High-quality articles related to mechanism of angiogenesis regulated by Notch signaling were included, and repetitive studies were excluded. RESULTS AND CONCLUSION:Totaly 31 literatures were selectively included in this review. Angiogenesis is a complicated and muti-stage biological event and has been delicately regulated. Notch signaling participates in the regulation of every stage of angiogenesis directly or indirectly via the crosstalk with vascular endothelial growth factor, BMP-SMAD signaling and extracelular matrix molecules. Furthermore, consistent output of Notch signaling is essential for maintaining the stability and integrity of adult vascular system. This review wil thoroughly clarify the latest progress on angiogenesis regulated by Notch signaling and provide theoretical basis and a potential target for designing therapeutic methods against vascular disease.

Objective To observe the impact of different culture conditions on the growth of the endothelial progenitor cells(EPCs) from rat peripheral blood. Methods Mononuclear cells obtained from rat peripheral blood were isolated by using density gradient centrifugation. According to the culture medium added with vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF) and the dishes were precoated with fibronectin or not, the mononuclear cells were divided into different groups and cultured under different conditions in vitro to compare the differences of the growth conditions. The different growth conditions were recorded and the result was calculated by statistics software. At last, the cells were identified by immunohistochemistry and immunofluorescence. Results The mononuclear cells from rat peripheral blood grew in the manner of keeping close to the wall in vitro. The cells and cell colonies cultured for 7 days hinted:under the same culture conditions, precoating FN was beneficial to the adherent proliferation of EPCs (t=4.43, P＜0.05; t=3.70, P＜0.05). Excluding the impact of fibronectin, growth factors could promote mononuclear cells differentiation to EPCs, indicating that growth factors enhanced proliferation of EPCs (t =-13.22, P＜0.01; t =-10.96, P＜0.01). Immunohistochemistry and immunofluorescence showed that, at day 4, 7, 10 of cells cultured, CD34 and CD133 expressions were increased gradually [(35.7±4.2)%, (60.1±3.8)%, (81.8±6.4)%; (3.2±0.9)%, (18.4±7.3)%,(32±3.8)%, respectively]; at day 14, both were decreased [(32.1±5.4)%, (1.9±2.7)%]; but Flk-1was increased at day 4, 7,10, 14 [(31.2±3.5)%, (40.6±5.3)%, (71.2±8.4)%, (81.5±4.1)%].Conclusions Fibronectin is conducive to adhesion and proliferation of EPCs. VEGF and bFGF play an important role in the differentiation of EPCs. The success culture of EPCs in vitro will provide a sufficient number of seed cells for its application in vascular tissue engineering, and offer new ideas for peripheral blood stem cell transplantation for the treatment of various diseases.

Misoprostol, a prostaglandin E1 analogue, have variety clinical applications. In early 80's, misoprostol is currently licensed primarily to prevent non-steroidal anti-inflammatory drug-associated gastric and duodenal ulcers. Further work shows the drugs have protection to hepatic and renal and prevention of inflammatory and allergic disorders. When combined with mifepristone,complete abortion rate is 95%. On the other hand, misoprostol is also an effective agent for cervical ripening and labor induction. It have no serious side effect, as a result, misoprostol is a drug of high effect and low toxicity, deserving popularization, but man and pregnant woman must prudent.