Objective To explore the effect of vasoactive intestinal peptide ( VIP) on the prevention and treatment of experimental autoimmune encephalomyelitis(EAE) rats by regulating the levels of IFN-γand IL-17A in brain tissue. Methods Sixty healthy female Wistar rats were randomly divided into 4 groups:normal control group, EAE control group, VIP low-dose group and VIP high-dose group.Myelin basic protein ( MBP)+complete Freurd′s adjuvant ( CFA) was used to establish an EAE model.The low and high-dose VIP groups were intraperitoneally injected with VIP 4 nmol/kg(0.2 ml) and 16 nmol/kg (0.8 ml) respectively every other day,while normal control group and EAE group with 0.8 ml saline for ten consecutive days.The incubation period, progression and peak of neurological dysfunction score ( NDS) changes of rats were recorded.The pathological changes, the GFAP+astrocyte activation in the brain at the morbidity peak of rats and the cytokine levels of IFN-γand IL-17A in brain tissue were observed.Results The incubation period was extended, the progression and peak NDS were shortened in the two VIP groups.In normal control group, there was no inflammatory cell infiltration or active astrocytes in brain tissue.The degree of infiltration of inflammatory cells and the degree of astrocyte activation in the VIP control group were significantly lower than in the EAE group.The cytokine levels of IFN-γand IL-17A in brain tissue were reduced in VIP groups.Conclusion By lowering IFN-γand IL-17A content in brain tissue, the infiltration of inflammatory cells and astrocyte activation are inhibited.VIP plays an important role in prevention and control of EAE.

Objective To explore the Caprini risk assessment model for the application of the prevention of VTE in clinical nursing. Methods With a cross:sectional survey research, 6 624 patients were interviewed of a hospital from March to June in 2015.The Caprini risk assessment model was used to screen patients with VTE risk assessment. Results Patients have VTE risk in 6 229 cases, accounting for 94.04%. The patients of low risk, moderate risk, high risk, and super high-risk was 1 889 cases, 1 507 cases, 1 930 cases, 903 cases, accounted for 28.52%, 22.75%, 29.14%and 13.64%respectively. Surgical patients with VTE risk, low risk, moderate, high risk and super high-risk rate was 5.15% (79/1 533), 15.72%(241/1 533), 28.77%(441/1 533), 30.59%(469/1 533), 19.77%(303/1 533), the no-surgery group were 6.21% (316/5 091), 32.37% (1 648/5 091), 20.94% (1 066/5 091), 28.68% (1 461/5 091), 11.79%(600/5 091), two groups compare was statistically significant (χ2= 53.46, P = 0.000); High-risk ratio of trauma surgery,thoracic surgery, neurosurgery, orthopedics, mammary gland and thyroid surgery were63.2%、62.9%、61.5%、58.3%、55.8%; High-risk ratio of oncology medical, respiratory medical, neurology medical, cardiology medical were 92.0% , 68.2% , 55.9% and 64.4% . Conclusions Hospitalized patients have high risk of VTE, it is suggested that include VTE in nursing quality evaluation standard, establish an effective system of VTE risk management. Caprini risk assessment model has been clear about the VTE patients should be special attention, and has been great significance to early screening and prevention of VTE.

Professor YANG Wen-hua has gradually formed her unique academic view and style on myeloproliferative disorders(MPD) during the past 30 years.She summed up and applied the diagnosis and treatment system of'liver heat and blood stasis,purging liver and eliminating stasis,flexibly in clinic.Method of integration of traditional Chinese and western medicine was adopted and got satisfactory effects,which can delay the disease process and transformation,improve the clinical symptoms,complications and quality of life,and prolong survival time.

Objective To evaluate the effects of percutaneous balloon mitral valvuloplasty (PBMV) on repeated rheumatism attacks after continual anti rheumatic treatment in vain over a long period of time.Methods 23 cases received treatment by PBMV and follow up visits were performed.Results After operation,patients' cardiac function and their physique were improved obviously and the activity of rheumatism ceased after routine anti rheumatic treatment and prevention of streptococcal infection.Follow up visits for 36～81 months showed that mitral re stenosis occurred to only 2 patients.Conclusion Patients with mitral stenosis whose rheumatism attacks repeatedly in spite of longterm anti rheumatic treatment may be treated with PBMV first and then given anti rheumatism.In this way,a better curative effect can be obtained.

[Objective]To observe and analyze the clinical results of ossification of posterior longitudinal ligament(OPLL) combined with high-signal intensity zone(HIZ) change. [Methods]Forty patients with OPLL combined with HIZ were treated from March 2004 to March 2008.Anterior approach surgery was given to 19 cases as group A and posterior approach surgery was given to 21 cases as group P.All cases were followed up.The pre-and post-operative JOA17 scores and improvement ratio were evaluated.[Results]The mean follow-up time were(19.5?3.4) months in group A and(20.3?4.5) months in group P.The mean improvement ratio were(56.2?8.4)% in group A and(52.2?12.2)% in group P.There were no statistical differences between those of groups A and P.Mean JOA scores at last follow-up were(12.8?0.9) in group A and(11.5?1.6) in group P,with significantly difference.[Conclusion]Treatment of OPLL combined with HIZ is a high risk procedure with limited recovery in neoro-function.Good surgical result could be got with correct judgement of type and location of OPLL,occupation ratio of spinal canal and selection of approach.

Objective: To investigate the relationship between serum uric acid(SUA) and prevalence of hyperuricemia(HUM) and the effects of dietary factors on HUM . Methods: Total 748 subjects from Guangzhou were questioned and SUA and body mass index (BMI) was measured by trained investigators. Results: The mean SUA concentration was (384.6?74.8)mmol/L in males vs (286.3?65.3) mmol/L in females, (P

Objective To investigate the relationship between carotid arteries atherosclersis and subclinical hypothyroidism in patients with type 2 diabetes mellitus (T2DM).Methods From January to December 2010,408 patients with T2DM were enrolled in this study.The patients were divided into euthyroidism group (376 patients) and subclinical hypothyroidism group (32 patients).The incidence of carotid arteries atherosclerosis in two groups was compared and the risk factors were analyzed by Logistic regression analysis in patients with T2DM.Results There was no significant difference in age,course of disease,body mass index,fasting plasma glucose,total cholesterol,high density lipoprotein cholesterol (HDL-C),low density lipoprotein cholesterol,triacylglycerol and glycosylated hemoglobin between two groups (P ＞ 0.05).The incidence of carotid arteries atherosclerosis in subclinical hypothyroidism group was higher than that in euthyroidism group [81.2％ (26/32) vs.61.4％ (231/376)],and the difference was significant (P =0.026).Logistic regression analysis showed that age and thyroid stimulating hormone (TSH)were risk factors (OR =1.178 and 1.227,P =0.000 and 0.019) and HDL-C was protective factor (OR =0.284,P =0.003) in T2DM with carotid arteries atherosclerosis.Conclusion Higher incidence of carotid arteries atherosclerosis is found in T2DM patients with subclinical hypothyroidism and increased TSH are independent risk factors that has not previously been described.

Objective To study the clinical effects on patients with brain metastases from lung cancer combined with Elemene Injection and radiotherapy. Method 86 patients with brain metastases from lung cancer were randomly divided into control group and treatment group, 43 cases in each group. Control group was given conventional radiotherapy treatment, while treatment group combined Elemene Injection with radiotherapy. Results Compared with control group, treatment group’s disease control effect was obviously better. Survival rates after treatment for six months, one year, three years were higher than control group, and the number of adverse reactions were lower. Serum MMP-2 and MMP-9 levels were significantly greater. Radiotherapy plan time and total time in hospital for treatment were significantly shorter. Conclusion The combination of Elemene Injection with radiotherapy treatment for patients with brain metastases from lung cancer is very obvious.

Ebosin is a novel exopolysaccharide produced by Streptomyces sp.139 with remarkable activity against rheumatic arthritis in vivo. In this paper, we reported effects of Ebosin on the inflammatory cytokines including interleukin-1beta (IL-1beta), interleukin-6 (IL-6) and tumor necrosis factor alpha (TNFalpha) in THP-1 cells. With the special fluorogenic peptide as substrates, the enzymatic activities of interleukin-1beta converting enzyme (ICE) and TNFalpha-converting enzyme (TACE) were inhibited by Ebosin separately. Using the real-time reverse transcription polymerase chain reaction (real-time PCR), the mRNA synthesis of the three cytokines were identified decline separately by Ebosin. The secretion quantum of three cytokines in THP-1 cells with Ebosin was lower than that of normal THP-1 cells determined by ELISA assay and Western blotting. All of these results showed that Ebosin has remarkably suppressed synthesis of the three cytokines in THP-1 cells through different pathways. The primary study of Ebosin on anti-inflammation mechanism was promoted developing the new drugs treating rheumatic arthritis.

Glaucoma is a neurodegenerative disease, which is characterized by a concave atrophy of the optic disc and a characteristic defect of the visual field.The current diagnosis and evaluation of glaucoma are mainly based on fundus photography, optical coherence tomography and visual field examination.Hemodynamic changes play an important role in the pathophysiology of glaucomatous optic nerve damage, however, blood flow evaluation of the optic disc has been directed to large vessels in the past, and there has been no good evaluation equipment for quantitative evaluation of blood flow in microvessels.Optical coherence tomography angiography quantifies blood flow in the retina and choroid, which provides a new method and index for the evaluation of glaucomatous optic nerve damage.In this paper, the application of optical coherence tomography angiography in the measurement of blood vessel density in the optic disc and macular region in the diagnosis and progress assessment of glaucoma, and the consistency of blood flow parameters with structural parameters such as the optic nerve fiber layer and sieve plate and visual field parameters, etc.were reviewed to evaluate the value of blood flow parameters in predicting and assessing glaucomatous damage.