1.Analysis of the effect of managing the proper use of antimicrobial agents
Wenying XUE ; Yiping ZHANG ; Fengjun HU
Chinese Journal of Hospital Administration 1996;0(01):-
At present improper use of antimicrobial agents in China is rather prevalent. For this reason we started from January 2000 to take general management methods and corresponding intervention measures with regard to the use of antimicrobial agents. We then made a survey of the use of antimicrobial agents before and after the adoption of the above methods so as to evaluate the effect of the intervention measures.
2.Clinical Study on Ginger-partitioned Moxibustion in Improving Blood Flow Velocity in Lower Limbs of Post-stroke Patients
Xue ZHANG ; Yanqun HU ; Rui ZHAO
Shanghai Journal of Acupuncture and Moxibustion 2015;(5):406-408
Objective To observe the clinical efficacy of ginger-partitioned moxibustion in improving blood flow velocity in lower limbs of post-stroke patients.Methods Ninety post-stroke patients were randomized into a treatment group and a control group, 45 in each group. The treatment group was intervened by ginger-partitioned moxibustion, while the control group was by conventional acupuncture. Before and after intervention, the blood flow velocity in lower limbs, National Institute of Health Stroke Scale (NIHSS) and modified Barthel Index (MBI) were evaluated.Results After 4-week treatment, the maximum blood flow velocity (Vmax) was significantly changed in the treatment group (P<0.05), and there was a significant difference in comparing the Vmax between the two groups after intervention (P<0.05); the NIHSS and MBI scores were significantly changed after intervention in the treatment group (P<0.05), and there was a significant difference in comparing MBI score between the two groups after intervention (P<0.05).Conclusions Ginger-partitioned moxibustion can accelerate the blood flow velocity in lower limbs of the post-stroke patients, improve blood circulation and the activities of life.
3.EFFECT OF RABBIT RETICULOCYTES 5srRNA ON DNA SYNTHESIS OF MOUSE MYELOMA CELLS SP2/0
Yan XU ; Xue HU ; Peixuan ZHANG ; Shepu XUE
Acta Anatomica Sinica 1954;0(02):-
5srRNA was isolated and purified from the reticulocytes of rabbit. It labeled with ~(125)I, then incubated with mouse myeloma cells (SP2/0). By autoradiography it was observed that ~(125)I-SsrRNA could pass into the nuclei of the cells. In a separate experiment, it showed that the incorporation rate of ~3H-TdR into nuclei of SP2/0 after incubation with 5srRNA was decreased as compared with that f control group, hence the result indicates that 5srRNA inhibits DNA synthesis of the SP2/0 cells and it seems to play a role in the regulation of gene expression through its hybridization with DNA sequences of the SP2/0 cells. Thus it is likely that 5srRNA might act as "erythroid denucleation factor".
4.3D-TOF MRA in the Etiologic Diagnosis of Hemifacial Spasm and Trigeminal Neuralgia
Jibo HU ; Shizheng ZHANG ; Xingyue HU ; Jingbing XUE ; Xianli ZHU
Journal of Practical Radiology 2000;0(12):-
Objective To study the clinical significance of 3 dimensional time of flight magnetic resonance angiography(3D-TOF-MRA) for the pathogenesis of hemifacial spasm (HFS) and trigeminal neuralgia(TN).Methods 48 patients with HFS and 46 patients without HFS and 42 patients with TN and 40 patients without TN were examined by MRI and 3D-TOF-MRA by the enhancement of DTPA. Diagnosis of the presence of compressions in the root exit zone(REZ) of facial nerves and trigeminal nerves were done by two radiologists on an independent console. Results (1)In the patients, compression of the REZ of the facial nerves and trigeminal nerves were detected on 45 spastic sides (93.8%,neurovascular on 44 sides and tumor on 1 side) and 36 spastic sides ( 85.7%,neurovascular on 32 sides and tumor on 4 sides ), 8 and 4 on the asymptomatic sides (16.7% and 9.5%, all neurovascular ). In the controls, 4 and 5 sides ( 4.4% and 6.3% ) were found in the compression of the REZ of the facial nerves and trigeminal nerves. ( 2 ) The offending vessels of compression of the REZ of the facial nerves were the anterior inferior cerebellar artery (AICA) in 17 cases ( 38.6% ), the posterior inferior cerebellar artery (PICA) in 12 cases (27.3%), the vertebral artery (VA) in 6 cases (13.6%). The offending vessels of compression of the REZ of the trigeminal nerves were the superior cerebellar artery ( SCA ) in 18 cases ( 56.3% ), the anterior inferior cerebellar artery in 5 cases (15.6%), the difficult identified vessels (DIV) in 4 cases (12.5%). (3)The relative risks of microvascular compressions which cause HFS and TN were 26.6 and 9.84. (4) The compressions of the REZ of the facial nerves and trigeminal nerves were proved in 4 cases (neurovascular 3 cases and tumor 1 case) and 10 cases (neurovascular 6 cases and tumor 4 casee) in the operation.Conclusion MRI and enhanced 3D-TOF-MRA appeare to be the best imaging technology for the pathogenesis of HFS and TN now. The major causes of HFS and TN may be different neurovascular compressions in the REZ of the facial nerves and trigeminal nerves, some cases are caused by tumor compression.
5.Dual-energy CT lung perfusion imaging and perfusion scintigraphy in rabbit models of acute peripheral pulmonary embolism
Xue CHAI ; Longjiang ZHANG ; Yuxiao HU ; Yane ZHAO ; Yangbo XUE ; Xiaobo HU ; Hong ZHU ; Guangming LU
Chinese Journal of Medical Imaging Technology 2010;26(2):221-224
Objective To explore the clinical value of dual-energy CT lung perfusion imaging and perfusion scintigraphy in acute pulmonary embolism (PE) rabbit models. Methods Acute PE models were established in 20 rabbits with femoral vein injection of sponge as embolus materials, and 4 rabbits were injected saline as control group. Two hours after embolization, dual-energy CT and perfusion scintigraphy imaging were performed respectively. Taking the pathologicall findings as the reference standards, the sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of both imaging methods were compared. CT values and enhancement degree (overlay value) of the embolic areas and normal pulmonary parenchyma were measured in DEPI image after embolization. Results Normal lung was color-coded as homogeneously yellowish red, perfusion scintigraphy displayed rarefaction or absence. The sensitivity, specificity, PPV, NPV of DEPI was 100%, 97.50%, 95.24%, 100%, and of perfusion scintigraphy was 67.50%, 81.25%, 64.29%, 83.33%, respectively. Increased CT values and overlay values of embolism areas in DEPI images were lower than those of normal pulmonary parenchyma (P<0.05). Conclusion DEPI has higher sensitivity and specificity than perfusion scintigraphy for detection of pulmonary emboli in an acute PE rabbit models.
6.Effects of different concentrations of recombinant human erythropoietin on proliferation of neural stem cells cultured in vitro
Zhengmin XUE ; Meng HU ; Changhai ZHANG ; Xiancheng ZHANG ; Xiaopeng ZHOU
Chinese Journal of Tissue Engineering Research 2011;15(23):4194-4198
BACKGROUND: Recombinant human erythropoietin (rhEPO) is a glycoprotein. Recent studies have demonstrated that rhEPO regulates many functional activities of neural cells. OBJECTIVE: To investigate the effects of different concentrations of rhEPO on proliferation of neural stem cells (NSCs) cultured in vitro. METHODS: Newborn Sprague-Dawley rat NSCs were harvested and cultured with serum-free culture medium containing different concentrations (5, 50, 500 U/mL) of rhEPO and 20 μg/L basic fibroblast growth factors (5, 50, and 500 U/mL rhEPO groups) and serum-free culture medium only containing 20 μg/L basic fibroblast growth factors (control group). After 7 days of culture, the cloning efficiency of NSCs was calculated. After 10 days of culture, neuron specific enolase (NSE)-and glial fibrillary acidic protein (GFAP)-immunoreactive cells were quantified. RESULTS AND CONCLUSION: In the rhEPO groups, cells proliferated rapidly, and the number of NSC microspheres was greater, in particular in the 50 U/mL rhEPO group, compared with the control group. NSCs grew faster in the 50 U/mL rhEPO group than in the control group. The number of NSE-and GFAP-immunoreactive cells was greater in the 50 U/mL rhEPO group than in the control group (P<0.01). These findings suggest that rhEPO promotes the in vitro culture and proliferation of NSCs, in particular 50 U/mL rhEPO.
7.Mechanism and clinical progress of molecular targeted cancer therapy.
Hong-xiang HU ; Xue-qing WANG ; Hua ZHANG ; Qiang ZHANG
Acta Pharmaceutica Sinica 2015;50(10):1232-1239
Molecular target-based cancer therapy is playing a more and more important role in cancer therapy because of its high specificity, good tolerance and so on. There are different kinds of molecular targeted drugs such as monoclonal antibodies and small molecular kinase inhibitors, and more than 50 drugs have been approved since 1997. When the first monoclonal antibody, rituximab, was on the market. The development of molecular target-based cancer therapeutics has become the main approach. Based on this, we summarized the drugs approved by FDA and introduced their mechanism of actions and clinical applications. In order to incorporate most molecular targeted drugs and describe clearly various characteristics, we divided them into four categories: drugs related to EGFR, drugs related to antiangiogenesis, drugs related to specific antigen and other targeted drugs. The purpose of this review is to provide a current status of this field and discover the main problems in the molecular targeted therapy.
Angiogenesis Inhibitors
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Antibodies, Monoclonal
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Drug Delivery Systems
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Humans
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Molecular Targeted Therapy
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Neoplasms
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drug therapy
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Protein Kinase Inhibitors
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Receptor, Epidermal Growth Factor
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antagonists & inhibitors
8.Modified procedure for the histochemical demonstration of myosin ATPase
Tao ZHANG ; Qian ZHANG ; Xijie HU ; Qian XUE ; Daixiong CHEN
Journal of Xi'an Jiaotong University(Medical Sciences) 1981;0(02):-
Objective To study the effect of self-modified Guth & Samaha's myosin-ATPase staining method on the classification of SD rat skeletal muscle fiber types.Methods 8 ?m-thick cryostat sections from rat chest muscles and biceps brachii muscles were transfected with cryostat.Myosin-ATPase staining was carried out according to the following procedure: ① Fixing sections for 5 min in fixative solution containing 40 g/L paraformaldehyde;② Rinsing slides in Tris-rinse solution and then preincubating them in alkaline preincubation solution for 15 min;③ Rinsing slides in Tris-rinse solution twice and then incubating them for 60 min in incubation solution;④ Washing slides for three times in 10 g/L CaCl2 and then placing them in 20 g/L CoCl2 for 3 min;⑤ Washing sides in distal water and then placing them in 10 g/L(NH4)2S for 3 min and;⑥ Washing slides in running tap water for 3 min,dehydrating in graded ethanol,clearing in xylene and mounting in neutral balsam.Results After being stained by modified myosin-ATPase staining method,both chest muscle and biceps brachii muscle samples from SD rats could be clearly identified as type Ⅰ fibers and type Ⅱfibers as the fibers of type Ⅰ were stained white while the fibers of type Ⅱ were stained brown.Conclusion Modified myosin-ATPase staining method is a simple and effective way for muscle fiber type classification and can be applied in skeletal muscle related study.
9.Ultrasonic Characteristics of Complex Fibroadenoma of Breast and Its Differential Diagnosis from Invasive Ductal Carcinoma
Meilian ZHANG ; Hu CHEN ; Ensheng XUE ; Liwu LIN ; Yimi HE
Chinese Journal of Medical Imaging 2017;25(5):365-369
Purpose To explore ultrasonic characteristics of complex fibroadenoma (CF) of breast and its differential diagnosis from invasive ductal carcinoma (IDC).Materials and Methods Sixty-one patients with CF and 81 patients with IDC from January 2010 to February 2016 in the Affiliated Union Hospital of Fujian Medical University were enrolled,and the CF and IDC were confirmed by pathology after operation.The preoperative sonographic findings of CF and IDC were retrospectively analyzed and compared.Results Fifty-nine cases with CF were detected by ultrasonography in 61 cases.Ultrasonic findings of 59 cases of CF showed as follows:79.7% (47/59) of the CF cases with an aspect ratio <0.7;79.7% (47/59) with blood flow signal of 0 to 1 level;72.9% (43/59) with liquid dark area,including 67.8% (40/59) with round or oval cystic fluid area showing local sievelike or diffused distribution;72.9% (43/59) with irregular shape;61.0% (36/59) with obscure boundary;61.0% (36/59) with the largest diameter of tumor <2 cm;59.3% (35/59) with calcifications,including 33.9% (20/59) with coarse calcification,25.4% (15/59) with microcalcification and 85.7% (30/35) with distribution of calcified lesions along the linear high echo zone;54.2% (32/59) with echo heterogenicity showing linear high echo and high echo in the middle and low echo.There were significant differences in the detection rates of internal regular fluid area,internal linear high echo or high echo area,and alcification distribution along the linear high echo between CF and IDC lesions (all P<0.01).Compared with IDC,there were higher rates in CF for detecting lesions of largest diameter of tumor <2 cm,more regular shape,more clear border,poorer blood flow signal (P<0.05),but there were lower rates in CF for detecting axillary lymph node enlargement,lesions with edge or spiculation and hyperechoic halo (P<0.01).Conclusion Color Doppler ultrasonography is of great value in the diagnosis of CF and its differential diagnosis from IDC.
10.Clinical value of MRI in early evaluation of the neonatal purulent meningitis
Dan ZHANG ; Jianhua FU ; Xindong XUE ; Jing GUO ; Yi HU
Chinese Pediatric Emergency Medicine 2011;18(3):217-219,229,后插1
Objective To analyze MRI manifestation of the neonatal purulent meningitis retrospectively,including conventional MRI and diffusion-weighted imaging (DWI),and to evaluate the value of MRI in early assessment of the neonatal purulent meningitis.Methods From Jul 2004 to Jul 2009,20 full-term newborns (14 male,6 female) from Shengjing hospital were included in this study,all of which were diagnosed as purulent meningitis.MRI and DWI examinations were performed within 72h after the diagnosis.Results (1) Among those 20 infants,19 infants (95%) had positive findings,including 7 cases of cerebral infarction,4 cases of subdural effusion,3 cases of encephalomalacia,1 case of hydrocephalus,1 case of ependymitis and 7 cases of intracranial hemorrhage.(2) Among the 7 cases of infarction,5 cases showed hypointensity on T1WI and hyperintensity on DWI and T2WI,but the other 2 cases only showed hyperintensity on DWI.Conclusion MRI can detect infarction and hemorrhage in the early stage of purulent meningitis.So MRI,especially DWI,is very important for the early diagnosis and evaluation of neonatal purulent meningitis.