Objective To evaluate the effects of cilostazol on plasma platelet-related indicators in trauma fractures patients with postoperative venous thrombosis.Methods 104 cases of trauma fractures patients with postoperative venous thrombosis from February 2012 to July 2013 in Laiwu City People’s Hospital were collected and randomly divided into group A and group group B,each had 52 cases Two groups were given conventional nursing, and group A were added cilostaeol group B were added aspirin.Other 50 cases of current healthy people for medical examination were selected for controls (group C).The effects of cilostazol on plasma platelet-related indicators in each treatment group were analyzed. Result Before treatment,the levels of IL-6 and IL-8 in group A and B were significantly higher than group C(P<0.01),but there is no difference between group A and B.The plasma CRP, FIB and DD in group A and B were significantly decreased than group C(P<0.01,P<0.05).After appropriate treatment,the levels of CRP,FIB and DD in group A and B were significantly reduced than pre-treatment(P<0.01,P<0.05).CRP content in group A was lower than that in group B,the difference was statistically significant(P<0.05 ),FIB and DD in group A showed no differencewith group B.Before treatment,PDW,PLT,MPV and P-LCR in group A and B were significantly higher than group C(P<0.01,P<0.05).After appropriate treatment,the levels of PDW,PLT,MPV and P-LCR in two groups were significantly decreased (P <0.01,P <0.05 ),but there was no difference between two groups.Before treatment,the expression of platelet activation in group A,B were significantly higher than group C(P<0.01),but significantly reduced after appropriate treatment (P<0.01),and the difference between the two groups was not statistically significant.Conclusion Cilostazol could reduce the expression of inflammatory factors,levels of D-dimer and fibrinogen and platelet related indicators by reduced platelet activation,which plays antithrombotic effect.

To investigate the effects of Tongxinluo capsule on sciatic nerve apoptosis in spontaneous type II diabetic KK/Upj-Ay mice, in order to explore its mechanism for improving diabetic peripheral neuropathy (DPN). KK/Upj-Ay mice were selected as the DPN animal model and randomly divided into the model, Tongxinluo low, middle and high group (1, 2, 4 g x kg(-1)). C57BL/6 mice were selected as the control group. Mice were given intragastrically for 12 weeks. Paw withdrawal latency, motor nerve conduction velocity (MNCV) and sensory nerve conduction velocity (SNCV) were detected. Apoptotic rate were detected by FCM. Bcl-2, Bax, Caspase-3 mRNA and protein expression in sciatic nerve were examined by Real-time PCR and Western blot. p38MAPK, p-p38MAPK expression were examined by Western blot. In this study,the authors found that Tongxinluo capsule could increase paw withdrawal latency, MNCV and SNCV. Apoptotic rate of sciatic, the expression of Bax and caspase-3 were lower, while Bcl-2 expression was higher in Tongxinluo group than those in model mice. The expression of p-p38MAPK significantly decreased in Tongxinluo group. The results showed that Tongxinluo capsule has protective effects on diabetic peripheral neuropathy of mice via inhibiting cell apoptosis and suppressing the expression of p-p38MAPK.
Animals ; Apoptosis ; drug effects ; Capsules ; administration & dosage ; Diabetic Neuropathies ; drug therapy ; physiopathology ; Disease Models, Animal ; Drugs, Chinese Herbal ; administration & dosage ; Humans ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; Sciatic Nerve ; cytology ; drug effects

Objective To develop a high performance liquid chromatography method for the determination of methylisothiazolinones and chloro-methylisothiazolinoe in cosmetics. Methods The cosmetic samples were extracted with methanol. The analytes were separated with the mobile phase of V(Methanol)∶V(Water)= 25∶75 at the flow rate of 1.0 ml/min and detected at the wavelength of 276 nm. Results The linear range of the method was 0.05-100 mg/ml for methylisothiazolinone and 0.0125-25 mg/ml for chloro-methylisothiazolinoe. The limit of detection of methylisothiazolinone and chloro-methylisothiazolinoe were 0.08 and 0.2 ?g respectively. The recovery rates were 84.5%-93.9% with the RSD of 2.5%-9.4% (n=6) for methylisothiazolinone and 88.3%-92.9% with the RSD of 2.9%-5.8% (n=6) for chloro-methylisothiazolinoe. Conclusion The method is simple,rapid,accurate and is suitable for the determination of methylisothiazolinone and chloro-methylisothiazolinoe in cosmetics.

Objectives To establish an HPLC method for determination of sodium Danshensu, hesperidin and salvianolic acid B in Niaosaitong capsules. Methods Three components were separated by C18 chromatographic column with a gradient mobile phase consisting of acetonitrile and 0.1%phosphoric acid solution at a flow rate of 1.0 mL/min, column temperature of 30 ℃, and detection wavelength was set at 286 nm. Results Sodium Danshensu, hesperidin and salvianolic acid B had good linearity in the range of 0.038 44-1.922 μg, 0.000 207 052 8-4.044 μg, 0.034 28-1.714 μg respectively, and the average recovery rate was 90.9%-107.1%, RSD was 2.1%-3.2%. Conclusion The methods is simple and accurate, and can be used for the quality control of Niaosaitong capsules.

Objective To investigate the present situation and outcome of cardiopulmonary resuscitation (CPR) for pediatric in-hospital cardiopulmonary arrest and to analyze the prognostic risk factors.Methods Data were collected from October 2008 till October 2011 using Ustein style.Patients older than 28 days who received CPR were evaluated.Returning of spontaneous circulation(ROSC) more than 24 hours was the primary outcome.Neurological outcome was assessed by pediatric cerebral performance categories half year after discharge.Results Of the 36 patients,15 (41.7％) achieved sustained ROSC.Seven (19.4％) patients survived to discharge.Single factor analysis indicated that the beginning heart rhythm,defibrillation and original disease were significantly different between the two groups(P ＜0.05).The beginning heart rote of the patient in ROSC ＞ 24 h group was mostly sinus bradycardia.Patients who need defibrillation had bad prognosis.Patients with heart disease had a lower rate of ROSC ＞ 24 h.At half year follow-up study,4 patients had 1 or 2 score,1 patient had 4 and 1 had 5 score in the pediatric cerebral performance categories scales.Condusion The successful rate of CPR in our hospital was the same as that in developed country.The beginning heart rhythm,defibrillation and original disease were associated with the outcome.Most of the patients who survived to discharge had a good neurological outcome.

Mortieralla isbellina (AS 3.3410) was used as the starting strain in the experiment. Through fermentation, the production rate of fungal body was 10%, content of lipid in fungal body was 27%, content of ?-Linolenic acid in lipid was 3.3%, When it was treated by U.V, mutant M_6 was obtained, its production rate of fungai body was 25%, lipid content was 32.8%, ?-linolenic acid content was 8.44%. Pass-generation test indicated that the heredity character of M_6 was stable.The optimum culture medium was obtained through culture condition test, and the result of fermentation in 10L tank showed that the production rate of fungal body was 29.3%, lipid content was 44.7%, GLA content was 9.44%.In the meantime, the method of extraction of fungai lipid was primarily studied, which indicated. that it is preferable to use a combination of ethanol and hexane to extract alternately.

Three hundred cases of E type viral hepatitis were observed with 100 cases of A type as control. We held that the etiology and pathogenesisof E and A types of viral hepatitis were wind, dampness, heat, poisons and stasis, each with its predominance; For A type, its predominant etiology being wind, heat and stasis, while that of E type, dampness, poisons and stasis. For their therapy, the principle of "similar treatment for different diseases " should, adopted. After treatment, ACT, TB, and a markedly decreased, signs and symptoms markedly improved, Epr, IgG also improved to different extents, the cured rate ( rate of negative virus exam) being 80% and 78% for A type and E type respectively. The effect was especially good for simple infection, and rather poor for mixed infection type.

As the patient of hepatitis B and E overlapping infection of liver and gallbladder damp - heat type compared with the patient of simple hepatitis E of liver and gallbladder damp-heat type, attack was very rapid, jaundice exacerbated progressively and hepatic function damaged severely. Aftertreatment the state of illness restored more slowly and the duration of illness was obviously prolonged. At the same time, it was found that the biochemical indexes (hyaluronic acid, total bile acid) increased significantly at acute stage, and the indexes in the patient of overlapping infection in the time for recovering normality at restoration stage was longer than that in the patient of simple hepatitis E, with a significantly difference between the two groups. It is indicated that lesion of the liver in the patient of overlapping infection of hepatitis B and E was more severe than the simple hepatitis E, with no good prognosis.

Objective To improve the capability identifying the causes of dyspepsia. Methods Standardized questionnaires were used to investigate the 2623 dyspeptic outpatients among four hospitals in Guan-zhong area, Shaanxi Province. All of the patients were followed up for 4-24 weeks, and finally diagnosed according to consistent criteria. Results The frequency rate of functional dyspepsia (FD) was 50.5%, benign organic causes were present in 39.3% and malignant diseases accounted for the remaining 10.2%. In the patients who had marked alarm symptoms, the proportion of benign organic causes and malignant diseases rose to 48.3% and 29.8% respectively, significantly higher than that without alarm symptoms (33.9% and 2.7% respectively)(P

Injury of peripheral nerve is a so common complication in trauma, that it is important to improve regeneration to recover the function of peripheral nerve. This paper has reviewed the pathology after injury of peripheral nerve, condition of successful regeneration of peripheral nerve, experiment and clinical study of electrical stimulation promoting regeneration of peripheral nerve and the possible mechanism. The effect of electrical stimulation on promoting regeneration of peripheral nerve has been gradually accredited. However, further study is still needed to discover the many unknown field.