From 38 pieces of deep\|sea samples,a strain,DY\|A,which produces protease was isolated.It can grow at a wide range of pH and salt concentration and best at 10℃.The strain produced maximum protease activity after growth at 10℃,initial pH 10 0 and inoculation volume 0 5%for 48～72h on a shaker by speed of 200r/min.The optimum pH and temperature for the protease activity production are pH 10.0 and 40℃.The enzyme is alkaline protease.Some other properties of the strain and the protease were discussed in detail.

Objective To explore the roles of IκBα and TGF-β1 on airway inflammation in rats with chronic bronchitis induced by smoking and study the effects and mechanism of anti-inflammation of pretreatment with ambroxol. Methods Sixty male wistar rats were randomly divided into four groups: Normal dosage group, model group, high dosage group and low dosage group. The rats with chronic bronchitis were established by smoking. For high and low dosage group, rats were pretreated respectively with ambroxol group, rats were pretreated with normal saline through peritoneal injection as much as the low dosage group.After 76 days, the histopathologic changes stained in hemotoxylin and eosin (H. E.) of bronchopulmonary tissues were observed under opticalmicroscope, white cell counts and differential analysis were performed in BALF, the expression of IκBα and TGF-β1 were detected by immunohistochemistry. Results The pathological changes of model group were in consistent with that of chronic bronchitis, but the degrees were significantly reduced in high and low dosage groups. Compared with those in normal group, the white cell count and the neutrophilic granulocyte count of BALF in model group were significantly increased and the macrophagocyte count decreased, and the expression of IκBαwas significantly decreased(t =3.24,3.31,3.29,3.48, P ＜0. 05) and the TGF-β1 significantly increased (P ＜0. 05). Compared with those in the model group, the white cell count and the neutrophilic granulocyte count of BALF in high and low dosage group were significantly decreased and the macrophagocyte count increased, the expression of IκBαwas significantly increased (t =2. 86,2. 97,2. 92,3.52,2.42,2. 88,2. 58,3.48, P ＜0. 05) and the TGF-β1 significantly decreased (P ＜0. 05) . Compared with those in low dosage group, the expression of TGF-β1 was decreased and the expression of IκBαincreased in high dosage group (t =2. 82,3.64, P ＜0. 05). Conclusions Down-regulating the expression of IκBα and up-regulating of TGF-β1 may be involved in the process of airway inflammation in rats with chronic bronchitis induced by smoking. Ambroxol might have better effects on ameliorating airway inflammation by up-regulating the expression of IκBα and down-regulating of TGF-β1.

Objective To investigate the origin of prostatic blood-supply by digital subtraction angiography (DSA) and provide references for the arterial embolization of prostatic hyperplasia. Methods A total of 72 male patients with successful selective and super-selective prostatic arterial catheterization angiographies were observed and recorded the original arterial blood-supply of the prostate, number of the prostatic arteries and the predominant blood supply. Results The blood-supply of prostate is very complicated including the original prostatic arterial supply from the inferior vesicle arteries (69); the internal iliac arteries (63); the internal pudendal arteries (52); the inferior rectum arteries (29); the superior vesicle arteries (14). The predominant arterial supply include the internal iliac arteries (37); the inferior vesicle arteries (20); the internal pudendal arteries (6) and the inferior rectum arteries (2). Conclusion Arterial contrast medium angiography can correctly demonstrate and assess the original arterial supply of prostate, providing significant guidance for further interventional embolization.

Cardiomyopathies ; congenital ; Heart Ventricles ; pathology ; Humans ; Infant, Newborn ; Male ; Myocardium ; pathology

This study reported the analysis of plasma phospholipid metabolism of the rats and the pathological biomarkers between the type 2 diabetes model control group (MC) and the normal control group (NC). SD rats were randomly divided into 2 groups: NC and MC. To investigate state of plasma metabolite profiling in normal body, type 2 diabetes mellitus (T2DM) model group using UPLC-Q-TOF/MS which was used as analysis tool in this research. The compounds were identified by UPLC-Q-TOF/MS based on MS/MS fragment ions information, element composition in MassLynx 4.1 and the Lipid Maps database. The sign of two groups of samples in specific markers for screening was through a software package in R software (BioMark software). The results show that the pathological markers were mainly phosphatidylcholine (PC) and triglycerides (TG); the 2-acyl PC in the MC group was less more obviously than that in the NC group; high carbon number and high degree of unsaturation of the TG was reduced under the condition of type 2 diabetes. In the state of type 2 diabetes, metabolic changes occurred in rat plasma phospholipids obviously, which had a close relationship with the occurrence and development of T2DM.

BACKGROUND:In the deep caries treatment, the pulp capping agent used in indirect pulp can induce the formation of dentin bridge (the complex of dental pulp and dentin), to protect the pulp tissue from stimulus and save the pulp vitality, completely avoid the occurrence of complications, with a higher clinical success rate. OBJECTIVE:To evaluate the differences in clinical efficacy of five kinds of calcium hydroxide preparations. METHODS:Totaly 236 patients with deep caries were selected and randomized to undergo indirect pulp with the double-component calcium hydroxide powder (n=46), Metapex with one-component injection dosage form and the chemical curing calcium hydroxide preparation (n=47), Dycal with the chemical curing calcium hydroxide preparation (n=45), Ionosit-Baseliner (n=49) and Calcimol LC (n=49) with one-component and the light curing calcium hydroxide preparation. The status of the dental pulp, the restoration and the root tip were observed at 1, 6, 12 and 24 months after operation. RESULTS AND CONCLUSION: There was no significant difference between the five groups in the success rate of indirect pulp capping at 1, 6 and 12 months (P> 0.05). At 24 months, the success rate of indirect pulp capping in the double-component calcium hydroxide powder group was lower than that in the other four groups (P < 0.05), but there was no significant difference between the latter four groups (P > 0.05). Metapex, Dycal, Ionosit-Baseliner and Calcimol LC al have a good effect on indirect pulp capping to deep caries, which can be used as agents for long-term pulp protection.

Objective To analyze TORCH infection situation in 6 027 cases of pregnant women,puerperae and neonates.Methods The TORCH IgM and IgG was detected by using ELISA for 6 027 cases of pregnant women,puerperae and neonates,and the positive rates of the antibodies to variant TORCH pathogens were analyzed.Results The positive rates of HSVⅠ-IgM,HSVⅡ-IgM,RV-IgM,TOXO-IgM and CMV-IgM were 0.02%,0.02%,0.12%,0.12% and 0.20% respectively.The positive rates of HSVⅠ-IgG,HSVⅡ-IgM,RV-IgM,TOXO-IgM,and CMV-IgM were 63.32%,13.31%,52.83%,12.68% and 58.57% respective-ly.Positive rate of CMV-IgM in neonates was higher than that in pregnant women,the difference was statistically significant(P <0.05).There were totally 28 cases of neonataes detected with acute CMV infection.Conclusion TORCH screening in pregnant women,puerperae and neonates helps the detection of infection disease in perinatal period.

BACKGROUND:Temporary crown has been used widely in clinic. The physical properties and biological safety of temporary crown and cement materials are more superior along with the development of material science. OBJECTIVE:To summarize clinical application, physical properties and biological safety of the temporary crown and cement materials. METHODS:PubMed database was searched by the key words of temporary crown, provisional crown, temporary restoration, provisional restoration, interim restoration in English to retrieve relevant articles published from January 2004 to December 2014. Literatures addressing the temporary crown and cement materials were included, and the repetitive researches were excluded. RESULTS AND CONCLUSION:Through the clinical evaluation and experimental study, the physical properties (polymerization shrinkage, polymerization heat production, polymerization monomer release) and biological safety (cytotoxicity, irritation to the soft tissue) of the temporary crown and cement materials are the key problems needed to overcome. With the appearance of new materials and new fabrication methods, the temporary crown and adhesive materials have great application prospect and higher clinical value, but in the exploration process of the laboratory research and clinical application, there are many problems to be solved.

BACKGROUND:In the comprehensive treatment of cracked teeth, the temporary protection using the orthodontic band and resin temporary crown can effectively prevent tooth fracture and protect the cracked teeth with a higher clinical success rate. OBJECTIVE:To evaluate the differences in clinical efficacy of orthodontic band and resin temporary crown on the temporary protection of cracked teeth. METHODS: Totaly 189 patients (97 males and 92 females, aged 19-65 years) with cracked teeth were selected, randomly divided into two groups and respectively underwent the temporary protection to cracked teeth with the orthodontic band (n=93) and resin temporary crown (n=96) in the comprehensive treatment. Retention effect, periodontal status and patient satisfaction were observed. RESULTS AND CONCLUSION:There were three cases with loose band and one case with faling band in the group of orthodontic band; there were two cases with faling band in the group of resin temporary crown. No significant difference between two groups in the retention effect was found. There were 11 cases of gingivitis or exploring hemorrhage in the group of orthodontic band and 2 cases in the group of resin temporary crown, indicating the periodontal status in the group of resin temporary crown was better than that in the group of orthodontic band (P< 0.05). There were 18 cases of lower satisfaction for poor color, expand feeling caused by the rubber band and discomfort caused by the band protruding from tooth surface in the group of orthodontic band; there were 3 cases of lower satisfaction in the group of resin temporary crown, and the patient satisfaction in the group of resin temporary crown were higher than that in the group of orthodontic band (P < 0.05). There is a better clinical effect in the temporary protection to cracked teeth by using the resin temporary crown, especialy in the terms of retention effect, periodontal status and patient satisfaction.

Adult ; Aluminum Silicates ; toxicity ; Humans ; Middle Aged ; Mining ; Occupational Exposure ; Prevalence ; Silicosis ; epidemiology