Objective: To study the clinical manifestations of the head and neck angiosarcoma and the re-lationship of the staging with clinical treatment and prognosis. Methods: The clinical data of 21 patients with head and neck angiosarcoma treated in our hospital between January 1993 and January 2008 were retro-spectively analyzed. By studying the medical records of all patients and reviewing the related literatures, we discussed the clinical manifestations of these patients and the relationship of staging with clinical treatment and prognosis of head and neck angiosarcoma based on soft tissue sarcoma staging of AJCC2002' 6th. SPSS 17.0 software was used for statistical analysis. We calculated the survival rate using Kaplan-Meier method and compared the survival curves using log rank test. Results: Among these 21 cases, 10 were treat-ed with non-combined treatment, and the other 11 cases were treated with combined treatment. There were 6 stage Ⅰ cases, 3 stage Ⅱ cases, 1 stage Ⅲ case, and 11 stage Ⅳ cases. The 1-, 3-, 5- and 10-year total sur-vival rates were 72.2%, 41.3%, 27.5% and 13.8%, respectively. The average time of recurrence or metastasis was 4 months. Twelve cases had local recurrence (accounting for 57.1%), and 11 cases had distant metasta-sis (accounting for 52.4%). The metastatic sites included lymph node in 5 cases, lung in 4 cases, liver in 1 case, and bone in 1 case. Different treatment modalities (χ~2=8.31, P=0.004) and tumor staging (χ~2=9.74, P=0.002) were prognostic factors. Conclusion: There are no unified treatment strategies for angiosarcoma of the head and the neck. The overall prognosis is still not ideal. Biological treatment has been applied to clinical practice. Preoperative combined treatment has positive results. Tumor staging is an important factor affecting the prognosis. Surgery-based combined treatment can improve patient survival.

Base Sequence ; Carcinoma, Medullary ; genetics ; surgery ; Codon ; genetics ; Exons ; genetics ; Female ; Humans ; Middle Aged ; Mutation ; Proto-Oncogene Proteins c-ret ; genetics ; Thyroid Neoplasms ; genetics ; surgery ; Thyroidectomy

BACKGROUND:There are two ways to treat the enamel before bracket bonding: etching and sandblasting, but the few studies focus on the direct use of sandblasting technology on untreated enamel surface.
OBJECTIVE:To observe the damage of etchingversus sandblasting to the enamel surface, and to compare the bonding strength of metal brackets adhesive to isolated teeth with these two kinds of surface treatments.
METHODS:(1) Nine premolar teeth removed for orthodontic treatment were randomized into three groups: sandblasting, acid etching and polishing treatment groups. Surface roughening effects of these three kinds of treatments were observed under scanning electron microscope. (1) Another 40 premolar teeth removed for orthodontic treatment were randomized into two groups: sandblasting and acid etching groups. At 24 hours after bracket bonding, the shear strength was detected using mechanical testing machine, and the adhesive residue index of tooth surface was statisticaly calculated.
RESULTS AND CONCLUSION: (1) Under the scanning electron microscope, polishing treatment had no damage to the enamel surface; but in the other two groups, the enamel surface was damaged to varying degrees, especialy in the sandblasting group. (2) The bonding strength in the sandblasting group was significantly higher than that in the acid etching group (P < 0.05), but there was no difference in the adhesive residue index of tooth surface between the two groups (P > 0.05). These findings indicate that compared with the acid etching technology, the sandblasting technology can increase the bonding strength between the enamel and metal bracket, but it also results in more damage.

Objective To observe the changes of type 2 diabetes mellitus patients in blood sugar,TC,TG,HDL C,plasma insulin,body mass index(BMI), microalbuminuria,etc before and after moderate intensity exercise training.Methods 28 patients with mean age of 59.0?7.6 years did moderate intensity exercise training for 1～1.5 hours every day for 12 month.At the same time they received the treatment of diet and oral antidiabetic drug com pared with 40 controls without exercise training. Before and after the observing period we measured the values of items above.Results Plasma glucose level , HbAlc,AUCGS(the area under the curve of glucose sugar) and BMI in fat diabetes with training team were obviously decreased compared with those before observation( P 0.05).Conclusion Exercise training can significantly descend blood sugar, insulin resistance and BMI of obese people ,improve lipidmetabolism and ascend insulin sensitivity. So the action of exercise can't be replaced by the diet and oral drug.

Objective:to study cognitive impairment and social function of patients with Alzheimer disease (AD). Method:22 patients with AD and 12 with depression in elderly were followed for 3 months, both at the very beginning and the end of 3 months, they were tested with CASI (cognitive abilities screening instrument) and FAQ (functional activities questionnaire).Result:scores of short memory, attention, mathematics, abstract and association of CASI were significantly lower in AD group than that in depression group. AD patients had also poorer outcome in FAQ, such as in that of shopping, learn new things, and recall of engagement.Conclusion:Cognitive impairment and social function of patients with AD were poorer than elderly patients with depression.

Objective To evaluate correlations between left atrial(LA) and left ventricular(LV) function in patients with hypertension by acoustic quantification(AQ) technique. Methods Forty-eight hypertensive patients and twenty control subjects were studied. Patients with hypertension were divided into two groups by left ventricular mass index(LVMI),normal LVMI group(32 cases) and left ventricular hypertrophy(LVH) group(16 cases). Left atrial ejection fraction(LAEF),atrial emptying volume(AE),conduit volume(CV) and reservoir volume(RV) were measured with AQ technique. Results RV and AE significantly increased in normal LVMI subgroup and LVH subgroup;CV and LAEF respectively decreased or increased in LVH subgroup. LA booster pump function positively correlated to onset atrial emptying volume and RV,negatively correlate to CV and LV diastolic function. LA conduit function positively correlated to LV diastolic function,negatively correlated to LAEF and AE,RV as well as onset atrial emptying volume. LA reservoir function positively correlated to onset atrial emptying volume and LAEF,negatively correlated to CV. There was no significant correlation between LA reservoir function and LV diastolic function. Conclusions LV diastolic dysfunction induces decreased LA conduit function and increased reservoir function,which may facilitate early diastolic filling of the left ventricle. The changes of LA conduit function and reservoir function may increase of LA preload. Enhancement of LA preload and LA systole makes increased LA booster pump function,which facilitate late diastolic filling of left ventricle.

Objective To explore the effect of bone marrow stromal cells (BMSC) transferred by Nell-1 gene combined with biomaterial fibrin glue (FG) to enhance segmental bone defect healing in dog mandible. Methods Nell-1 gene vector was reconstructed in retroviral vector and then transfected BMSC. The protein of Nell-1 gene in transferred cells was determined by immunohistochemistry. Segmental defects were created surgically in the dog’s mandible. The defect was repaired with BMSC transfected by Nell-1 retroviral granules in presence of FG,untransfected BMSC in combination with FG,and FG alone. The control group was left untreated. The defect-repairing capability for each treatment were assessed by gross observation,radiography,and histology at 8th week and 16th week. Results Cells transfected by Nell-1 retroviral granules expressed abundant Nell-1 mRNA and protein in the cytoplasm. Positive results were not found in those cells that were not transferred. The use of BMSC transferred by Nell-1 retroviral granules combined with FG materials exhibited the strongest defect-repairing ability. Gross observation,radiographical and histomorphometric analyses revealed a significantly greater total area of bone formation,increased amount of the new bone in the defects than in those treated with the untransfected BMSC. Conclusion Nell-1 gene transfection may be used to promote the osteogenic ability of BMSC.

As a tumor suppressor, p53 is activated by numerous cellular and environmental signals, and plays a criticalrole in the cell cycle regulation, cell apoptosis and senenscence. The murine double minute (MDM)2 and double minute mu?rine 4 (MDMX) are two important regulators. MDMX is a p53 binding protein with strong sequence homology to MDM2, but lacks ubiquitin ligase activity, and which is unable to target p53 for proteasomal degradation. MDMX regulates p53 activity through its binding with p53 and its postranscriptional modification. MDMX in the closed and open structure binds to p53 to regulate its activity. As the main partner of MDMX, casein kinase 1 alpha (CK1α) disrupts the intramolecular binding in MD?MX in the cooperation to regulate p53 activity. The process of MDMX and CK1αin the regulation of p53 is multi-step and complicated. In this paper the mechanism of MDMX and CK1αin the regulation of p53 protein was reviewed.

Objective To clone the human bone morphogenetic protein-2(hBMP2) gene cDNA and construct its replication-deficient type 5 adenoviral vector.Methods Using osteoblastic sarcoma tissue to extract cell total RNA,hBMP-2 cDNA was amplified by RT-PCR.The hBMP-2 cDNA was then inserted into cloning vector pGEM-T.The recombinant plasmid was screened and the recombinant plasmid which was confirmed by restriction enzyme,PCR and sequence analysis was gotten.To construct the recombinant adenoviral vector,the BMP-2 cDNA was subcloned into a shuttle vector and the recombinant pShuttle-BMP2 vector was obtained.It was subcloned into Adeno-X adenoviral genomic DNA and the pAd-BMP2 DNA was obtained.Results A length of 1 200 bp BMP-2 cDNA was cloned,and it was subcloned into adenoviral vector,the recombinant adenovirus was constructed successfully.After measuring the titre of virus(1?1011 nfu?L-1),the target gene was evaluated by PCR.Conclusion The hBMP-2 adenoviral is obtained.The successful construction of pAd-BMP2 may provide us useful target for the further research.

Objective:To compare the choriocapillaris flow density and choroidal vessel volume (CVV) of acute and chronic central serous chorioretinopathy (CSC).Methods:A cross-sectional observational clinical study. A total of 64 eyes of 64 patients (CSC group) diagnosed with CSC at Department of Ophthalmology of West China Hospital of Sichuan University from May 2019 to October 2020, and a total of 64 eyes of 64 age and gender matched healthy volunteers (control group) during the same period were included in this study. In the CSC group, there were 34 patients with acute CSC (acute CSC group) and 30 patients with chronic CSC (chronic CSC group). There was no significant difference in age ( t=-0.041) and sex composition ratio ( χ2=0.191) between CSC group and control group ( P>0.05). There were statistically significant differences in age ( t=-1.872) and sex composition ratio ( χ2=8.778) between acute CSC group and chronic CSC group( P<0.05). Swept source optical coherence tomography angiography (SS-OCTA) was performed using VG200D. The scanning mode was 512×512 and scannig range was 12 mm × 12 mm. The choriocapillaris flow density of the 3 mm, 6 mm, 12 mm circular area and 1-3 mm ring, 3-6 mm ring, and 6-12 mm ring, and the CVV of the of the 3 mm, 6 mm, 12 mm circular area was automatically generated by the built-in software (v1.28.6). The age, choriocapillaris flow density and CVV were compared between two groups using independent sample t test. Results:Compared with the control group, the choriocapillaris flow density decreased in the CSC group, and there were statistically significant differences in the 3 mm, 6 mm circular area ( t=-7.210, -4.040; P<0.001). There were statistically significant differences between CSC group and control group in the 3 mm, 6 mm, 12 mm circular area ( t=1.460, 12.270, 11.250; P<0.05). Compared with the acute CSC group, the choriocapillaris flow density decreased in the chronic CSC group, and there were statistically significant differences ( P<0.05) in the 3 mm, 6 mm circular area ( t=3.230, 2.330), the total and four quadrants of 1-3 mm ring ( t=2.780, 2.060, 2.140, 2.620, 3.770), the superior quadrants of the 3-6 mm ring ( t=2.550), and the superior and temporal of 6-12 mm ring ( t=3.070, 2.610). There was no significant difference of CVV in the 3 mm, 6 mm and 12 mm circular area between the acute CSC group and the chronic CSC group ( t=0.250, 0.070, -0.110; P>0.05). Conclusion:Compared with acute CSC, chronic CSC exhibits significant decreased choriocapillaris flow density and no change in CVV.