Objective To explore the expressions and significances of apoptotic modulating genes Fas and Bcl-2 mRNA in peripheral blood monocytes(PBMC)of patients with myasthenia gravis (MG). Methods The levels of Fas and Bcl-2 mRNA in PBMC were detected by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) in 44 patients with MG as well as 30 healthy donors served as a normal control (NC).Results (1) The level of Fas mRNA in MG group was no significantly difference compared with NC group. The level of Bcl-2 mRNA in MG group was higher than that in NC group(P

Cathepsin S is one of the cathepsins that distributes generally at lysosomes of histiocyte in humans body.It has variety of actions when it is unbalancing,including promote inflammation,atherosclerosis,diabetes and obesity,etc.Studies in recent years have demonstrated that there are certain correlations of adiponectin with vascular risk factor and cerebrovascular disease.

Objective To determine whether rapid and delayed tolerance to focal cerebral ischemia can be induced by pretreatment with single electroacupuncture (EA) at the Baihui acupoint in rats. Methods Forty-eight healthy male SD rats weighing 280-320 g were randomly divided into 6 equal groups with 8 animals in each group: (1) control group received no pretreatment before focal cerebral ischemia; (2) electroacupuncture group was further divided into 5 groups in which EA was performed at Baihui acupoint and electric stimulation was maintained for30min0.5 h(EAl),1 h(EA2),2 h(EA3),3 h(EA4) or 24 h(EA5) before focal cerebral ischemia. The animals were anesthetized with isoflurane and spontaneous respiration was maintained. Middle cerebral artery occlusion (MCAO) was performed using a nylon thread with a rounded tip inserted into internal carotid artery and thread cranially until resistance was felt. MCAO was maintained for 2 h, then the nylon thread was withdrawn to allow reperfusion . After MCAO the animals were observed for 24 h for neurological deficits which were assessed by a 0-5 grade scoring system (0 = no deficit, 5 = death) . The animals were then sacrificed and brain was immediately removed for estimation for the size of cerebral infarction. Results There were significantly less neurological deficits and the size of infarction was smaller in group EA3 and EA5 than those in control group. There were no significant differences in neurological deficits and size of infarction between group EA1,EA2,EA4 and control group. Conclusion Pretreatment with single electroacupuncture stimulation for 30 min at Baihui acupoint can induce rapid and delayed tolerance to focal cerebral ischemia. Rapid tolerance develops 2 h after pretreatment while delayed tolerance develops 24 h after pretreatment.

Objective To evaluate the effective treatment for and prevention of complications. Methods The diagnosis and treatment of 32 patients with acute arterial ischemia of the lower extremity were analyzed (retrospectively). 4 cases were treated with thrombolytic therapy, 28 cases underwent emergency operation, of which 3 cases underwent amputation after embolectomy, and 25 cases were treated by arteriotomy of femoral artery and embolectomy with Fogarty catheter(7 cases underwent concomitant arterioplasty).Results One (patient) in the nonoperative group died; but there was no mortality in the operated group. Limb amputation was done in 3 cases, and limbs were saved in 28 cases. Conclusions For acute arterial ischemia of the lower (extremity), emergency arteriotomy of femoral artery plus embolectomy is the treatment of choice. This (therapeutic) approach can lower mortality and limb amputation rate.

0.05).The maximum relaxant forces of vitrification and fresh arteries groups were greater than the freezing group(P 0.05).The arteries of three groups began obvious relaxant responses when sodium nitroprusside was 10-7 mol/L, and nearly reached their maximal relaxant responses when sodium nitroprusside was 10-4 mol/L.CONCLUSION:The vitrification could preserve more active smooth muscle cells of arteries than the freezing method.The norepinephrine has better effect on contraction ability of artery, which preserved in the vitrification group than the freezing group;however, there is no difference in relaxation of sodium nitroferricyanide.

The main obstacles and limitations on development of advanced life-rescuing information technology are identified based on analysis of existing information technology.The research ideas on digital information to replace analog information and response technology in lieu of new type life-rescuing information technology are putted forward,which combines RFID technology with biomedical engineering technology to research and design the life transponder that application bottleneck and advantage of the technique are analyzed.

Objective To investigate the teaching effects for students of seven-year program.Method Symposiums were hold among 3 batches for seven-years program and questionnaire collected.Results We need to further modify the curriculum and improve clinical teaching skills,and students also need to have more research ideas.Conclusion We should modify current teaching content,curriculum,and skills.Clinical skills and humanization management should be highlighted.This is the key to ensure that teaching effects and teaching quality can be improved effectively.

MicroRNA are endogenous non-coding small RNA composed of approximately 18 - 23 nucleotides, mainly participating in post-transcription gene regulation. More and more data show that miR-21 is over-expressed in many human cancer cells and it was involved in cell differentiation, proliferation and apoptosis, closely related to tumorigenesis and the development of carcinoma as oncogene. Here, we will make a review about miR-21' s target gene, biosynthesis and regulation and clinical significance in order to provide a valued data for the future study and clinical application.

Objective To detect COX-2 mRNA in cervical cancer and normal cervix, and explore the pathogenesis of adenocarcinoma of the uterine cervix. Methods COX-2 expression was detected by RT-PCR to explore the relationship between COX-2 and the adenocarcinogenon of cervical cancer in 36 cases of adenocarcinoma of the uterine cervix and 15 cases normal cervical tissue. Results The positive rate of COX-2 mRNA in adenocarcinoma of the uterine cervix tissues (55.6%) was higher than that of normal tissues ( 20. 0% ), the difference was significant ( P ＜ 0. 05). The expression of COX-2 mRNA was associated with stages and clinical grading of tumor ( P ＜ 0. 05), but no significant difference was found between stages and clinical grading of uterine cervix adenocarcinoma( P ＞ 0. 05). Conclusion The hyper expression of COX-2 mRNA in uterine cervix adenocarcinoma took part in the carcinogenesis of tumor.