Mesenchymal stem cells (MSC) are non-hematopoietic, multipotent progenitor cells which can be isolated from various human adult tissues. In recent years, MSC have been shown to possess broad immunoregulatory function and tissue regeneration. This review discusses mesenchymal stem cells in hematopoietic stem cell transplantation, including disorders as diverse as acute and chronic graft-versus-host disease, graft failure, pure red cell aplasia, autoimmune thrombocytopenic purpura, hemorrhagic cystitis.

Objective To investigate the influence of different head and neck positions on airway sealing pressure (OLP) with i-gel laryngeal mask airway (LMA) in patients undergoing elective surgery under general anesthesia with mechanical ventilation.Methods Thirty ASA Ⅰ or Ⅱ patients of both sexes aged 32-64 yr with a body mass index of 18-30 kg/m2 undergoing elective surgery under general anesthesia with LMA were studied.Size 4 i-gel LMA was placed blindly after induction of anesthesia and connected to anesthetic circuit.The criteria for successful placement were easy inflation with small leak,peak inspiratory pressure (PPeak) within normal range and normal PETCO2 wave form.Fiberoptic bronchoscopy was performed to check the position of LMA.OLP,expiratory tidal volume ( VTE ),PPeak and efficiency of mechanical ventilation were measured in different head and neck positions.OLP was measured by gradually increasing airway pressure until PPeak was reached.The measurement was stopped when there was large leak or OLP ＞ 35 cm H2 O.The efficacy of ventilation was evaluated by manually inflating the lungs (perfect:easy inflation,no audible leak; satisfactory:easy inflation,small leak; poor:difficult inflation,large leak).Results Flexion of the head significantly increased OLP and PPeak and decreased VTE,while hyperextension significantly decreased OLP and PPeak and increased VTE as compared with middle position of the head.Lung ventilation was less satisfactory when the head was flexed.Turning the head to either side did not affect ventilation.Conclusion Flexion of the head should be avoided when i-gel LMA is used during anesthesia.

China ; Health Surveys ; Humans ; Physical Fitness ; Students

Objective:To investigate the cause and preventive measures of chemotherapy-related death from human malignant neoplasms.Methods:A retrospective study was carried out to evaluate the clinical data of 15 patients with chemotherapy-related death of human neoplasms in 2008.The cause,treatment,survival time after chemotherapy and preventive measures were analyzed.Results:The average age of the 15 patients was 52.4 years,and 5 of them were treated with docetaxel.The mean survival time after chemotherapy was 16.8 days.5 cases presented severe sehematological toxicity, and 2,breath failure.Conclusion:Chemotherapy-related death from human malignant neoplasms was correlated with disease history,age and chemotherapeutic agents,etc.;therefore,no use of docetaxel for patients aged over 60 years or with hepatic and lung diseased history could decrease the chemotherapy-related death.

Objective To investigate the applied value of diffusion tensor tractography(DTT) and visual evoked potential(VEP) for monitoring lumpectomy of lesion adjacent to the optic radiation.Methods Six patients with intracranial lesions adjacent to the optic radiation performed MRI DTT before operation in order to know relationship between the optic radiation and lesion.Neuronavigation was performed and VEP was recorded continuously during the operation.Results Pathological results of specimens showed that 2 cases were astrocytoma(WHOⅡ),1 case was oligodendroglioma(WHOⅡ),1 case was oligodendro-astrocytoma(WHOⅡ),1 case was anaplastic astrocytoma(WHOⅢ～Ⅳ),1 case was cavernous angioma.No patients were found visual deficit and VEP of 6 cases were normal postoperatively.Conclusions Combination neuronavigation with optic radiation DTT preoperatively and neuronavigation with real-time VEP intraoperatively are reliable to monitor the visual function and is helpful to perform neurosurgical planning near the visual pathway to avoid vision postoperative dysfunction.

Objective To review and analyse the CT distinguishing features of pulmonary cryptococcosis (PC)for improving the level of image diagnosis of the disease.Methods CT images and clinical details of 19 patients with biopsy or surgery pathology con-firmed PC were reviewed and analysed.Results Nodules or masses were presented in 10 cases,in which most casescases were nod-ules,lesions ranged in diameter from 1 cm to 2.5 cm.Multiple nodules were easy to fuse.Consolidation were presented in 6 cases,with high attenuation and clear border.3 cases were mixed type,with nodule,mass consolidation and ground glass attenuation.The lesions were mostly located in the inferior lobes(47%)and peripheral lung(68%).The features of pleural thickening,air bronchus sign,halo sign and cavitations were observed in 42%,36%,26%,21% of patients respectively.Conclusion CT findings of PC are diversity.The lesions are mostly located in the inferior and peripheral lung.Lesions are mild to moderate enhanced.Pleural thickening,air bronchus sign,halo sign,cavitations will be useful for the diagnosis.

Objective :To investigate expression of E-Cadherin(E-CD) in breast cancer cells and its relationship to clinical and pathological character and to the incidence of tumor cells shed from the surgical field. In an attempt to study more deeply about the mechanisms of invasion and metastasis of breast cancer and give some reference to the diagnosis and treatment of breast cancer. Methods: The expressions of breast carcinoma cells were studied using immunohistochemical methods(ABC methods).Semi-quantitative methods were used to judge the results. For statistical analysis, Chi-square test and the exact probability in 2 ?2 table were used. Results : E-CD positive was located on cell membranes and cytoplasms, mainly on membranes, showing different intensity of brown pellet. Of 52 cases, twenty nine percent retained normal E-CD expression, seventy one percent have reduced or even none expression. E-CD expression has significant correlation with histological grade. Reduced and lost expression rate in Grade 1(36.4%,4/11) were much lower than grade 2(77.8%,28/36)and grade 3(75%,6/8) (p

Objective To evaluate the treatment effect of PFNA clinical analysis in patients with intertro-chanteric fracture application.Methods 40 cases of intertrochanteric fractures PFNA internal fixation treatment were selected,the operation time,intraoperative blood loss and fractures healing time,degree of hip joint function recovery after operation and the postoperative complications were observed.Results Operating time was 60 -120minutes,with an average of (90.0 ±12.5)minutes.Intraoperative blood loss volume was 200 -600mL,and the average volume was 380.8 plus or minus 58 ml.Fracture clinical healing time was 8 to 15 weeks,with an average of (10.5 ±2.5)weeks. According to Harris hip joint function score,the overall good rate was 85.5%.There were no postoperative complica-tions occurred.Conclusion PFNA applies to all types of intertrochanteric fractures,especially for the old type osteo-porosis bone fracture,which with simple operation,small trauma,fixed firmly,fracture healing fast and less complica-tions.It is worthy of clinical use.

Objective: To study the effects of static tensile strain on the cytoskeleton of human periodontal ligament fibroblasts (HPLFs). Methods: Tensile strain with the strain variate of 8%,12%,16% and 20% was applied in the culture of HPLFs for 1,2 and 3 d respectively with a self-devised loading apparatus. With the aid of laser scanning confocal microscope, the projected areas of each observed cell and its nuclear were calculated and the cell cytosketeton was observed. The data analysis was carried out with SPSS using Dunnett test. Results:The projected areas of the cells and nucleus incereased with the increace of loading time and tensile strain value in trial groups of 8%,12% and 16%(P

Objective:To observe the expression of nuclear factor-kappa B( NF-κB) in rats with acute lung injury ( ALI) caused by oleic acid ( OA) and investigate the protective effect of puerarin ( Pue) in ALI. Methods:Twenty-four rats were randomly divided into three groups:the control group, OA group and Pue group. Morphological changes in lung tissues were observed under a light mi-croscope and the expression of NF-κB in lung tissues were examined. Results:Morphological changes in lung tissues showed that pul-monary inflammation was relieved in Pue group compared with that in OA group. Compared with the control group, the expression of NF-κB was increased obviously in OA group (0. 39 ± 0. 07 vs 0. 12 ± 0. 04, P<0. 05), and that was decreased obviously in Pue group (0. 24 ± 0. 05), which was still higher than that in the control group (P<0. 05). Conclusion:Puerarin can alleviate the inflammation reaction in ALI through inhibiting the expression of NF-κB.