2.Direct Molecular Analysis of Garlic Using Internal Extractive Electrospray Ionization Mass Spectrometry
Hua ZHANG ; Liang ZHU ; Huanwen CHEN
Chinese Journal of Analytical Chemistry 2014;(11):1634-1639
Theapplicationofinternalextractiveelectrosprayionizationmassspectrometry(iEESI-MS)was extended to direct molecular analysis of garlic tissues. By obviating time-consuming sample preparations, fragile active garlic substances such as organosulfur compounds ( e. g. , alliin, allicin ) were successfully detected and identified via collision-induced dissociation ( CID) , together with amino acids ( e. g. , arginine) and saccharides ( glucose, polysaccharides) . Mass spectral fingerprints of different kinds of garlic cloves, as well as various post-treatment ones, were further processed via principal component analysis ( PCA) to better visualize the differences. Our experimental results indicated that iEESI-MS allowed rapid recognition of metabolic changes in the garlic tissue subject to various external stimuli. The merits included simplicity of analysis, high speed ( less than 2 min per sample ) , good specificity, and minimal disturbance to the bioactivity of analytes.
3.Scientific Research and Clinical Application of Stem Cell:a New Ethical Topic in Life Science Century
Chinese Medical Ethics 2015;(5):659-663
Based on the analysis on the most common type of scientific research and clinical application of stem cells, including embryonic stem cells , hematopoietic stem cells and mesenchymal stem cells as the breakthrough point, discusses common phenomenon and problems of ethics , scientific research and clinical study the moral life science century new topic, hope scientists, physicians, ethicists, caused by the administrative departments for pub-lic health thought , promote stem cells healthy and steady development .
4.Plasma Inflammatory Cytokines in Severe Acute Respiratory Syndrome Animal Model.
Hua ZHU ; Yangqing ZHANG ; Xinming TU
Journal of Medical Research 2006;0(03):-
Objective Severe acute respiratory syndrome is recently emerged infectious disease caused by a novel coronavirus, but its immmunopathological mechanism has not yet been fully elucidated. Method We established the SARS animel model and investigated changes in plasma inflammatoy cytokines monkeys and rats. 8 monkeys with PCR and antibody positive were detected. Serum levels of inflammatory cytokines interleukin(IL)-6,IL-10,Th-1 cytokine,interferon(INF)-? and tumor necrosis factor(TNF)-? were measured by enzyme-linked immunosorbent assays(ELISA).Result The concentration of IL-10 and TNF-?were not significantly different in model and control group.IL-6 showed marked elevation of at least 10 days, and there was a positive relationship between the level of IL-6 and pulmonary pathological changes. The INF-? level decreased. Conclusion The result of sera level of SARS animal model avoid the disturbance of anti-viral drug and corticosteroid, it suggest that there are different immunoregulatory events during SARS and may contribute to our understanding of the pathogenesis.
5.DIVERGENT COLLATERALS FROM FOREBRAIN AND BRAINSTEM TO THE DORSAL HIPPOCAMPUS IN THE RAT
Jinxia ZHU ; Xiangting ZHOU ; Hua ZHANG
Acta Anatomica Sinica 1955;0(03):-
Divergent axon collaterals from forebrain and brainstem to the dorsal hippocampus in the rat were examined by means of HRP method and fluorescent retrograde double labeling method.HRP solution was injected into the left or right dorsal htppocampus of 3 rats. HRP labeled cells were observed in the bilateral medial parts of medial septal areas, vertical limbs of the diagonal band of Broca, supramammillary nuclei, submammillothalamic nuclei, superior central nucleus and locus ceruleus.PI and Bb solution were injected into the left and right hippocampus of 3 rats separately. Many retrograde double labeled cells were found in the nuclei mentioned above.The results indicate that the retrograde double labeled ceils in these nuclei may divergently projected to both the left and right dorsal hippocampi. They play an important role in harmonizing the activity of bilateral hippocampi.
6.Analysis on Epidemiological Characteristics and its Related Risk Factors of Chronic Diseases among Adult Residents in Hebei Province
Junqing ZHU ; Hua LI ; Jianxin ZHANG
Chinese Journal of Prevention and Control of Chronic Diseases 2006;0(06):-
Objective To understand the epidemiological status of chronic diseases and its related risk factors among adult residents in Hebei province.Methods A total of 4 200 adults from 20 districts in Hebei province were selected by multistage stratified random sampling method,and they were investigated with questionnaire,and the body measurement and blood sample were analyzed.Results The prevalent rates of overweight,obesity,central obesity,hypertension,dyslipidemia and diabetes mellitus were 35.19%,16.38%,52.98%,38.50%,39.62% and 5.81%,respectively.The age-adjusted rates were 30.28%,14.05%,44.32%,27.86%,32.71% and 4.00%,respectively.The prevalence of chronic diseases was highly associated with age.The prevalence of chronic diseases in male was higher than that in female among people aged under 55 yrs,but lower among people aged over 55 yrs.The rates were higher in urban areas than in rural areas.The prevalence rates of cigarette smoking and alcohol-drinking in male were higher than in female(P
7.Research progress of subchondral bone in osteoarthritis
Hua ZHU ; Faming TIAN ; Liu ZHANG
Journal of Medical Postgraduates 2014;(10):1095-1098
Osteoarthritis ( OA) is a chronic joint disease characterized by degeneration of articular cartilage and changes of subchondral bone play an important role in the occurrence and development of OA .Recent studies have found that change in the struc-ture and mechanical properties of subchondral bone is one of the main pathological processes in OA .To confirm the role of subchondral bone in OA process can provide not only more details about the pathogenesis of OA , but also new targets for treatment .Early diagnosis and treatment of OA may be possible by detecting radiographic and genomics of subchondral bone .We review subchondral bone chan-ges andits role in OA process in aspects of biomechanics , biology, radiological and genomics .
8.Evaluate the value of anti-CCP antibody and RF in diagnosis of rheumatoid arthritis
Jiwen ZHU ; Xiaojun ZHANG ; Hua ZHU ; Baoping DAI ; Tao WANG
International Journal of Laboratory Medicine 2015;(18):2681-2682,2685
Objective To evaluate the effectiveness of using anti‐cyclic citrullinated peptide (anti‐CCP) antibody or rheumatoid factor(RF) as the single laboratory parameter for rheumatoid arthritis(RA) diagnosis ,versus to using the two parameters in com‐bination .Methods A total of 56 cases of patients with RA were enrolled into RA group ,other 34 cases of patients with out RA were enrolled into non‐RA group .Levels of anti‐CCP antibody and RF were detected by using electro‐chemiluminescence immunoas‐say(ECLIA)and compared between the two groups .And the diagnostic efficacy of single and combined detection of anti‐CCP anti‐body and RF were evaluated .Results The levels of anti‐CCP antibody and RF in the RA group were higher than those in the non‐RA group ,had statistically significant differences(P<0 .05) .In diagnosis of RA ,the areas under receiver operating characteristic (ROC)curve of anti‐CCP antibody and RF were 0 .925 and 0 .822 respectively .The sensitivity and specificity of anti‐CCP antibody were both higher than those of RF .Compared with single detection of anti‐CCP antibody ,there was no significant increase of sensi‐tivity in using anti‐CCP antibody/RF ,whereas the specificity dropped significantly .Combined detection of anti‐CCP antibody and RF ,compared with single detection of anti‐CCP antibody ,had significantly lower sensitivity ,but no significant changes were found in specificity .Conclusion Single detection of anti‐CCP antibody is more effective than RF for diagnosing RA ,while combined detec‐tion of anti‐CCP antibody and RF could not significantly improve the specificity and sensitivity .It is suggested to only use anti‐CCP antibody for diagnosis of RA .
9.Minute on the 5th Guangdong Provincial Conference of integrated medicine on deficiency syndrome and geriatrics.
Li ZHANG ; Rong-hua ZHANG ; Bing-kuang ZHU
Chinese Journal of Integrated Traditional and Western Medicine 2003;23(6):478-479
Aged
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Animals
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China
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Drugs, Chinese Herbal
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therapeutic use
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Humans
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Osteoporosis
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drug therapy
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Phytotherapy
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Stroke
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drug therapy
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Yang Deficiency
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drug therapy
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Yin Deficiency
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drug therapy
10.Nasal septal perforation combined with chronic invasive fungal rhinosinusitis: three cases report.
Hua-ming ZHU ; Wei-tian ZHANG ; Yu-jun ZHANG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2012;47(8):692-693
Aged
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Humans
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Male
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Middle Aged
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Mycoses
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complications
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Nasal Septal Perforation
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complications
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microbiology
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Sinusitis
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complications
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microbiology