Objective To discuss the relationship between matrix metalloproteinases 9 (MMP-9) and the pathogenesis of preeclampsia , birth weight and placental weight , through detecting the expression of MMPS-9 in the placenta of the patients with preeclampsia. Methods The levels of MMP-9 in the placenta were detected by immunohistochemical SP method in 60 patients with preeclampsia and 30 pregnancy women. Results (1) The optical density value of MMP-9 in preeclampsia group was lower than the control group , the optical density value of MMP-9 in SPE group was lower than the MPE group (P < 0.05). (2) The placenta weight and birth weight in preeclampsia group were lower than the control group , the placenta weight and birth weight in SPE group was lower than the MPE group (P < 0.05). (3) The expression level of MMP-9 were positively correlated with placental weight and birth weight (P < 0.05) in the control group. (4) The average optical density value of MMP-9 in neonatus with weight < 2 500 g was lower than neonatus with weight≥2 500 g (P < 0.05). Conclusion The MMP-9 in the placenta of patients with preeclampsia was down-regulated , and its expression was abate when the illness grew worse. The MMP-9 may be involved in the pathogenesis of preeclampsia , and it is associated with fetal growth restriction.

With the increased use of contrast agents, the incidence of contrast induced nephropathy has Contrast induced nephropathy has become the third most common cause of renal injury caused by the hospital , following renal hypoperfusion and renal toxicity. But its pathogenesis is not clear, and there is no specific treatment. Therefore, prevention is particularly important. Intravenous hydration is widely recognized as an effective method, but is not suitable for all the patients. More researches on the current drug prevention of contrast induced nephropathy provide possibility in individual prevention of contrast nephropathy.

With the increased use of contrast agents, the incidence of contrast induced nephropathy has become the third most common cause of renal injury caused by the hospital, following renal hypoperfusion and renal toxicity. But its pathogenesis is not clear, and there is no specific treatment. Therefore, prevention is particularly important. The method of remote ischemic preconditioning is simple, noninvasive and effective, and should be the first method.

Accidents, Occupational ; Acute Disease ; Aniline Compounds ; poisoning ; Humans ; Occupational Diseases

liver after i.v. administration. The distribution of osthole in blood and kidney were siginificantly higher( P

Objective To investigate the effects of various intensity of 1 Hz repetitive transcranial magnetic stimulation (rTMS) on up-per limbs motor function after stroke. Methods From December, 2014 to December, 2015, sixty patients with ischemic stroke were random-ly divided into high density group (HD, n=20), low density group (LD, n=20) and control group (n=20). They all received routine rehabilita-tion and medication. The HD group received rTMS over contralesional motor cortex twice a day, while the LD group received rTMS once a day, and the control group received sham stimulation, 10 minutes a time for 10 days. They were tested with motor-evoked potentials (MEPs), Fugl-Meyer Assessment (FMA) of upper limbs and modified Barthel Index (MBI) before and after treatment. Results The ampli-tude of MEPs of the affected cortex, the scores of FMA and MBI were more in HD and LD groups than in the control group after treatment (P<0.01), and were more in HD group than in LD group (P<0.01). No serious side-effect was found. Conclusion rTMS was feasible for pa-tients with stroke to improve the upper limbs motor function. It is more effective as more times a day.

Objective To evaluate the patient-controlled paravertebral block (PCPB) in optimizing the cellular immune function when used after radical resection of pulmonary carcinoma performed via video-assisted thoracoscope in patients.Methods Forty-one ASA physical status Ⅰ or Ⅱ patients of both sexes,aged 50-64 yr,with body mass index of 20-25 kg/m2,of TNM staging Ⅰ or Ⅱ,undergoing radical resection of pulmonary carcinoma performed via video-assisted thoracoscope,were randomly divided into 2 groups using a random number table:PCIA group (n =21) and PCPB group (n =20).PCIA solution contained sufentanil 2 μg/kg in 100 ml of normal saline.The PCIA pump was set up to deliver a 2 ml bolus dose with a 15-min lockout interval and background infusion at 2 ml/h.In PCPB group,the patients received paravertebral injection of 0.2％ ropivocaine 5 ml at T5 level on the affected side under ultrasound guidance at the end of operation,and then received PCPB.PCPB solution contained 0.75％ ropivacaine 67 ml in 250 ml of normal saline,and the pump was set up to deliver a 5 ml bolus dose,with a 15-min lockout interval and background infusion at 5 ml/h.VAS score was maintained ≤ 3,and analgesia lasted until 50 h after operation.Before induction of anesthesia (baseline),at end of operation,and at 1,3 and 5 days after operation,peripheral venous blood samples were collected to determine the levels of regulatory T cells,natural killer cells and natural killer T cells (by flow cytometry) and plasma concentrations of interleukin-10 and transforming growth factor-β (by ELISA).Results Compared with group PCIA,the level of regulatory T cells was significantly decreased,the levels of natural killer cells and natural killer T cells were increased,and the plasma concentrations of interleukin-10 and transforming growth factor-β were decreased at 1 and 3 days after operation,and no significant change was found in the rate of cellular immune function decline after operation in group PCPB.Conclusion PCPB provides no significant difference clinically in optimizing the cellular immune function when used after radical resection of pulmonary carcinoma performed via video-assisted thoracoscope in the patients.

Objective To investigate the altered expression of TLR4 in alveolar macrophages of diabetic rats after lipopolysaccharide (LPS) stimulation and the effect of these changes on defending the infection. Methods Thirty-two male Wistar rats were divided into 4 groups, group A: the control group; group B: the diabetic group; group C: the LPS stimulated group; and group D: the diabetic group with LPS stimulation. TLR4 in alveolar macrophages were measured by immunocytochemistry, RT-PCR and Western blot analysis. Results The expressions of TLR4 in group B and group C were higher than that in group A (P < 0. 001). The expression of TLR4 in group D was obviously higher than that of group B and group C (P < 0.001). Conclusion The expression of TLR4 of diabetic rats was higher than that of normal rats and became more higher after LPS stimulation, which is indicated that diabetic bodies were in the proinflammatory state, the mechanism remains to be explored in detail.

Objective To investigate the safety and clinical prognosis of breast conserving operation in treat-ment of triple negative breast cancer.Methods 157 patients with breast cancer were divided into the non triple nega-tive breast cancer group (n=76) and triple negative breast cancer (n=81) according to its receptor expression,clin-ical pathological features, local recurrence after 5 years.Distant metastasis and survival situation were compared between two groups.Results The age<35 years patients of triple negative group accounted 17.3%,significantly higher than 7.9%of the non triple negative group(χ2 =5.331,P<0.05);the size of tumor≥5cm of triple negative group accounted 79.0%,significantly higher than 59.2% of the non triple negative group(χ2 =7.022,P<0.05);The patients with positive family history and lymph nodes of the triple negative group were 9.9%and 28.4%,signifi-cantly higher than 2.6%and 17.1%of non triple negative group,the difference between the two groups was statisti-cally significant (χ2 =6.283,4.865,all P<0.05);The local recurrence,distant metastasis rate and 5 year survival rates between the two group were not significantly different(χ2 =2.559,2.829,2.361,all P>0.05);The 5 year dis-ease free survival rate of the triple negative group was 80.25%,significantly lower than 94.74%of the non triple neg-ative group (χ2 =6.572,P<0.05).Conclusion The patients with triple negative breast cancer is younger,their tumor size is large and has a high proportion of family history.Their recurrence rate,distant metastasis rate and 5 year overall survival rate are as better as the patients with non triple negative breast cancer after breast preservation opera-tion,which has high security and good clinical application value.

This paper described the practice of real-time booking registration, in which the patients may freely choose their visit time points, by such means as the outpatient clinic, website, telephone or short messaging. Such booking registration is an important measure to improve service quality at outpatient clinics; information technology is a prerequisite for real-time booking registration; pooling of outpatient ID number resources is key to setting up the booking registration; the prepaid card deposit practice makes booking registration more efficient; enhanced management for supporting services safeguards booking registration; cooperation with qualified third parties is an effective way to accomplish booking registration in a convenient manner. This paper also probed into other issues in relation to real-time booking registration.