Objective To explore the effect and intracellular signal transduction pathway of insulin-like growth factor 1 (IGF-1) on the expression of stem cell factor (SCF) in spesis rats colonic smooth muscle cells (SMC).Methods The model of spesis rats was established,colonic SMC from spesis rats were cultured by enzymolysis and identified by α-actin immunofluoresence methods.Colonic SMC incubated with different concentrations (0,50,100 and 150 μg/L) of IGF-1 for different durations (0,12,24,36 and 48 h),and another SMC were pretreated with specific mitogen-activated protein kinase (MEK) inhibitor PD-98059 and phosphatidylinostiol 3-kinase(PI 3-kinase) inhibitor LY-294002 before IGF-1 induced.The expression of SCF in colonic SMC was examined by Western blot and quantitative reverse transcription polymerase chain reaction.Results (1) A very low level of SCF was expressed in colonic SMC cultured in bovine serum free medium.The protein and mRNA were 0.223 ± 0.047 and 0.149 ± 0.023 respectively.The expression of SCF protein and mRNA from SMC was increased to 0.398±0.033 and 0.243±0.013(P＜0.05) when concentration of IGF-1 was 50 μg/L.The final effective concentration of IGF-1 in vitro was 100 μg/L,the levels of SCF protein and mRNA were increased to 0.719±0.017 and 0.360±0.006(P＜0.05).(2) For the time point induced the SCF expression by IGF-1,the expression level of protein and mRNA in 0 h were 0.347±0.031 and 0.092 ±0.018 respectively.The time point of SCF peak level occurred at the 24 h,the expression level of SCF protein and mRNA were increased to 0.893 ± 0.049 and 0.396 ± 0.048 (P ＜ 0.05).(3) The IGF-1-induced SCF expression was reduced significantly by a pretreatment of PD-98059 (SCF protein and mRNA were reduced 37％ and 45％,P＜0.05),and LY-294002 had no effect on the expression of SCF (P＞0.05).Conclusion The expression of SCF from colon SMC in sepsis rat is stimulated by IGF-1 in both dose and time-dependent manners,in which ERKMAPK signal transduction may play an important role.

Objective To study the clinical experience of pure laparoacopic left hemihepatectomy. Methods Pure laparoscopic left hemibepatectomy was attempted in 10 patients including 3 cases of primary hepatic carcino-mma,3 cases of cavernous hemangioma of liver,2 cases of hepatic cyst,and 2 cases of calculus of intrahepatic duct. Results Fully pure laparoscopic left hemihepatectomy was accomplished for all 10 cases. Operation time was 190-340(253.0±51.2) min,blood loss was 200-800(342.0 ± 191.7) ml,and hospital stay was(5.2 ± 1.3) d . No com-plicatious occurred. Conclusion Laparoscopic left bemibepatectomy is safe and feasible, which can be considered asan excellent choice for selected cases.

Retinoblastoma 94(RB94)gene,a new tumor suppressor gene,is found in the past several years.It has been found that Rb94 gene has a more powerful antitumor effection compared with Rb110gene.After transfection,Rb94 gene could adjust telomerase activation,induce apoptosis and regulate cell cycle.It also has a synergistic action associated with radiotherapy or chemotherapy.

The severe hand,foot and mouth disease(HFMD) caused by Enterovirus 71 (EV71) has become a serious threat to children's health.It is an important issues which need to be faced and resolved by pediatrician and disease prevention and control system,especially pediatric critical care specialist.The pathogenesis of severe HFMD caused by EV71 has not been clearly defined so far,and the clinical treatmnent is controversial.So,to further explore the mechanism of EV71 infection in severe HFMD and explore new effective prevention and control methods,have important significance in reducing the mortality and improve the quality of life of patients with severe HFMD.

Objective To study the expression of Sox2 in human prostate cancer and pericarcinomatous tissue,and the relation between its expression and the pathological type and clinical stage of prostate cancer.Methods Immunohistochemisty,Western blot and reverse transcription-polymerase chain reaction (RT-PCR)were used to detect the expression of Sox2 in 86 cases of prostate cancer tissues and 40 cases of pericarcinomatous tissues.The relation between Sox2 expression and the pathological type and clinical stage of prostate cancer was analyzed.Results The expression of Sox2 in prostate cancer tissue (71.9％) was significantly higher than that in pericarcinomatous tissue (7.5％),and the difference was statistically significant (x2 =53.98,P ＜ 0.05).Sox2 expression in prostate cancer tissues was significantly related to Gleason score (x2 =19.49,P ＜ 0.05).The positive rate of Sox2 in lymphatic metastasis group and negative lymphatic metastasis group was 92.3％ and 73.3％ respectively,and the difference was statistically significant (x2 =3.95,P ＜ 0.05).While there was no significant difference among clinical stages (x2 =0.16,P ＞ 0.05).Moreover,Western blot and RT-PCR revealed that the expression of Sox2 in prostate cancer tissue was significantly higher than that in pericarcinomatous tissue (t =-2.93,P ＜ 0.05 ; t =3.29,P ＜ 0.05).Conclusion The high expression of Sox2 is closely related to the Gleason score and lymphatic metastasis of prostate cancer,but not to the clinical stage.The overexpression of Sox2 may play an important role in formation,differentiation and metastasis of prostate cancer.

0.05).Conclusion The polymorphism of rs2285676 in KCNJ11 may not be associated with T2DM in Hanethnic population in Chongqing.

Objective:To investigate the effect of EBV protein on the production of Immunoglobulin(Ig)by cultured B cells.Methods:The purified human umbilical blood B cells were treated respectively with UV and Heat-inactivated EBV and cultured in complete IMDM. CD5, CD3, CD4 and CD8 cells were measured by flow cytometric analysis. IgG and IgM in the supernatant were detected by ELISA. At the mean time, IgG and IgM in the supernatant of cultured EBV-transformed B lymphocytes were detected by ELISA,and compared with that of UV-inactivated EBV group at the same period.Results:In UV-inactivated EBV group,CD3, CD4 and CD8 T cells couldn’t be detected,CD5 + cells occupied about 42% of all detected cells at the 14th day; and at the 28th day, 47%.The IgG A value of the 10th、18th、22th and 26th day in UV-inactivated EBV group have a significant difference compared with that in the control group ( P

ObjectiveTo study the feasibility of laparoscopic assisted splenectomy for massive splenomegaly and devascularization in the treatment of patients with cirrhosis and portal hypertension.MethodA retrospective study was carried out on 12 patients who underwent laparoscopic assisted splenectomy and devascularization from October 2003 to January 2010.ResultsThe operation was successfully carried out in eight patients.The remaining four patients were converted to open surgery due to intra-operative bleeding.The mean operation times for the 12 patients and the 8 patients who underwent laparoscopic surgery were 350 min (range,190-470 min) and 313 min (range,190-410 min),respectively.The corresponding mean intra-operative blood losses were 1300 ml (range,200-2200 ml) and 700 ml (range,200-1700 ml),respectively.For the 8 patients who received laparoscopic surgery,flatus were passed 3-5 days after operation,and the postoperative hospitalization was 10-20 days.ConclusionsLaparoscopic assisted splenectomy for massive splenomegaly and devascularization is feasible.It is a treatment option for patients with cirrhosis and portal hypertension.

Objective:To investigate the development of vestibular system function in 7 to 14 years old normal boys and the ADHD boys;Methods:The present study involved two groups of subjects:148 ADHD boys(age range 7 years to 14 years) diagnosed with DSM-IV diagnostic criteria and 148 normal boys with the same age.Each group was divided into 4 clusters according to two years gap.All subjects were tested for the vestibular system function by the Vestibular Function Test System 2000/ ENG-V600 with the eye movement index.Results:From 7 to 14 years,the development of vestibular system function in normal boys had the characters as the description below.The optokinetic system function exhibited more development from seven years in the normal group.From 7 to 10 years,the time delay of saccade and anti-saccade in normal boys was significantly shorter(In the normal controls,the delay time of left saccade was 149?66 ms in 7-8 years old boys,108?64 ms in 9-10 years old boys,117?72 ms in 9-10 years old boys,the delay time of left anti-saccade was 178?127ms in 7-8 years old boys,101?88 ms in 9-10 years old boys,P

Objective To observe clinical therapeutic effect of Yiqi Huayu Prescription for diabetes peripheral nerve and blood vessel pathological change. Methods One hundred patients with diabetes peripheral nerve and blood vessel pathological change were randomly divided into two groups. The patients in control group (n=48) were treated by routine treatment. The patients in treatment group (n =52) were treated by oral use of Yiqi Huayu Prescription and routine treatment. Their therapeutic effects were compared after three courses of treatment, which was 30 days. Results The total effective rate was 92.9% in the treatment group and 61.1% in the control group. There was significant difference between the two groups (P 0.05). The internal diameter of the dorsal pedal artery and popliteal artery and the peak value of flow rate of the treatment group were improved (P