BACKGROUND:The polylactic acid/poly plycolic acid (PLGA) exhibits excellent biomechanical property with poor cell adhesion. However,acellular cartilaginous matrix had good cell adhesion and hydrophilia,which can mediate signal conduction and interaction among cells,yet the biomechanical property,is poor. PLGA-acellular cartilaginous matrix scaffold is a remedy for their shortcomings,which can be a newly scaffold materials. OBJECTIVE:To observe the ability of PLGA,acellular cartilaginous matrix,PLGA-acellular cartilaginous matrix scaffold in supporting the growth of porcine chondrocytes for cartilage tissue engineering. DESIGN,TIME AND SETTING:The comparative observation experiment was performed at the Laboratory of Shanxi Medical University from March to September 2008. MATERIALS:The average aperture of PLGA is from 100 -200 ?m,with porosity rate of 94%. The average aperture of the acellular matrix scaffold ranged 70-100 ?m,with porosity rate of 85%. The average aperture of PLGA-acellular matrix aperture is 100-300 ?m,and the porosity rate is approximate 90%. METHODS:The experiment was divided into three groups according to the scaffolds:PLGA,acellular cartilaginous matrix,and PLGA-acellular cartilaginous matrix group. The free chondrocytes isolated from porcine knee articular were seeded onto 3 kinds of scaffolds after cartilage amplification,followed by 8 weeks culture in vitro. MAIN OUTCOME MEASURES:The expression of type Ⅱ collagen was detected by immunohistochemistry. The contents of mRNA of type Ⅱ collagen were determined by RT-PCR. Meantime,the content of DNA was measured by Hoechst 33258 method. RESULTS:Chondrocytes grows vigorous on the PLGA-acellular cartilaginous matrix scaffold,which can maintain phaenotype after 8 weeks of culture,and the secretion of type Ⅱ collagen was superior to other 2 groups. Meanwhile,the content of DNA and mRNA of type Ⅱ collagen of PLGA-acellularcartilaginous matrix groups were greatest,followed by acellular cartilaginous matrix group,and the content of PLGA was smallest. The one-factor analysis of variance showed the difference had significant (P

Images corresponded to the right knee of a 27 years old, healthy, male volunteer, weighing 68 Kg, 175 cm height, with no knee joint disease, were selected. The scanning was performed using 1.5T Twinspeed/Excite magnetic resonance tomography. The image was uninterrupted sagittal planes picture, and the scanning way was from the inside to out. The three-dimensional tibia-femoral joint model was established by the MR1 images after they were handled by correlation analysis software (Mimics10.0, Geomagic Studio 8.0). At last, transformed the model format STL file to IGS file, which can be acceptable by analysis software 11.0. On the base of this way, a highly simulation tibia-femoral model was established. It provides a guarantee for the further finite element analysis concerning checking the surface of femoral condyle cartilage to repair interphalangeal joint injuries. The result demonstrated that 3-D computer model established by using 2-D MRI pictures makes up the CT scanning deficiencies on soft tissue, which confirms the model closer to the real anatomical structure.

Objective To investigate expression change of Ku70 protein before and after radiotherapy in patients with cervical cancer, as well as its relationship with clinical characteristics. Methods Seventy cervical cancer patients who received three dimensional conformal radiotherapy combined with chemotherapy were registered. Specimens tissues were obtained from cervical tumors of irradiation dose of ≤10 Gy and ≥20 Gy group. Immunohistochemistry was used to detect the expression of Ku70 in 70 cases, and its relative expression was analyzed by image software. Results The relative expression levels of Ku70 in cervical cancer patients treated with radiation doses of ≥20 Gy group (1.31 ± 0.83) and ≤10 Gy group (0.71 ± 0.60) were significantly higher than that before radiotherapy (0.50 ± 0.19), and there was a significant difference (t=7.143, P< 0.01; t= 2.655, P< 0.05). The expression of Ku70 was related with clinical stage, differential degree and tumor diameter (all P< 0.05), but not with age and tumor type (both P> 0.05). The expression of Ku70 in radiotherapy resistant group was significantly higher than that in radiotherapy sensitive group (0.55 ± 0.24 vs. 0.25 ± 0.17, t= 2.979, P< 0.01). Conclusions The expression of Ku70 protein in cervical cancer tissues is increased with the rise of radiotherapy dose. The expression of Ku70 in radiotherapy resistant group is significantly higher than that in radiotherapy sensitive group, indicating that Ku70 protein is correlated with the radiosensitivity, clinical stage, differential degree and tumor diameter.

Objective To investigate the clinical curative effect and security of Kanglaite injection in combination with low power ultrasound microbubble agents (cavitation) in the treatment of vascular embolization therapy.Methods Thirty-eight patients with abdominal malignant tumor,in accordance with the random number table,were divided into two groups:treatment group (Kanglaite combined with ultrasonic cavitation,21 cases) and control group (Kanglaite,17 cases).Intravenous drip with Kanglaite injection for 21 days,200 ml per day.Ultrasonic cavitation therapy treatment for three weeks,5 days a week and once a day.Tumors size,Karnofsky score,grade of the degree of pain and blood biochemical indicator detection were measyred before and after treatment.Results There was no complete remission,4 cases with partial remission,10 cases with stable disease in the treatment group,and the clinical benefit rate was 66.7％ (14/21).There was no complete remission,1 case with partial remission,3 cases with stable diseasein in control group,and the clinical benefit rate was 23.5％ (4/17).The treatment group was better than control group in clinical benefit rate (66.7％ ∶ 23.5％),pain improvement (76.2％ ∶ 41.2％),Karnofsky score [(66.67 ± 5.77) ∶ (82.86 ± 6.44);(64.12 ±5.07) ∶ (69.41 ±6.59)],and one year survival rate (57.1％ ∶23.5％) (x2 =7.012,P =0.008;x2 =4.821,P=0.028;t=4.575,P＜0.001;x2 =4.354,P=0.037).Conclusion Kanglaite injection in combination with cavitation shows higher clinical efficient,tolerated adverse recations,and significant improvement of quality of life.

The setups of internal medicine and surgery, confronted with the challenges of the development of modern clinical sciences, must be regrouped so as to conform to the demands of technological development. The set ups of internal medicine and surgery have the following drawbacks in division: ①hindering the scientific research on modern clinical medicine for lacking the theoretical basis of preclinical medicine; ②making it difficult for diseases to get treated systematically; ③affecting innovations in advanced medical technology; ④resulting in a greater number of problems of medical control; ⑤leading to an increasingly worsening situation of hospital economy. It is, therefore, necessary to regroup departments of internal medicine and surgery, set up a mode of treatment by systemic diseases, and readjust the structure of human resources and medical regimens according to this mode. Other departments, such as gynecology and obstetrics, otorhinolaryngology, ophthalmology, hematology, and endocrinology, can be treated as independent medical setups.

0.05). CONCLUSION: Long-term biocompatibility of titanium alloy is better than stainless-steel, and there are no significant differences between crossing group and non-crossing group, which do not affect the healing of fractures.

We studied and reviewed the PDCA cycle theory related literature, and summarized the research related to PDCA cycle application in the research management in hospitals in china.We also analyzed the present problems and explored future direction of development.

Professor Zhang Yonghong thinks that the deficiency of lung qi always exists in advanced lung cancer cases. Pathogen infection, phlegm and blood stasis are key factors to the occurrence and development of lung cancer. Clinical treatment in advanced lung cancer should emphasize on the benefiting of qi and supplementing the lung as well as reducing phlegm, removing blood stasis and disintoxication. Attention should be paid to both syndrome differentiation and disease differentiation with the combination of strengthening the body resistance and eliminating evil factors according to different stages of lung cancer.

Objective To observe the efficacy and toxicity of S -1 monotherapy in elderly patients with advanced non -small lung cancer (NSCLC).Methods Fifty-six patients aged 70 or over with stage IIIb-IV NSCLC were enrolled.S-1 was given orally twice a day according to body surface area on days 1 to 14 for 21-day cycle, the evaluation of efficacy and toxicity were performed on all patients after 2 cycles.Results For all 56 patients, CR 0, PR 15, SD 29 and PD 23 were observed.The objective response rate (ORR) and the disease control rate (DCR) were 26.8% and 78.6%,respectively.The 1-year survival rate was 30.9%, with a medi-an survival of 10.0 months.The myelotoxicity and gastrointestinal toxicity were major side effects .The major toxicity could be tolerated by the patients.Conclusions S-1 monotherapy is an effective chemotherapy for advanced NSCLC in elderly patients with moderate side effects and safety in clinical practice .

Seed cell, biomaterials, constructing of tissue are three essential elements of tissue engineering. The core is the technology of tissue constructing. Bioreactor for tissue engineering is a sort of systematic equipment constructing ttissues in vitro. Cardiac tissue engineering may hold great potentials for not only replacing or supporting an infarcted cardiac tissue but also treating and saving the lives of patients with heart diseases. This paper mainly introduced the progress of domestic and international technology for constructing engineered cardiac tissue in vitro, especially in the research of bioreactors used for engineered cardiac tissue construction.