Objective To analysis the hot spots of innovative technology of Chinese medicine industry in Shanghai during the recent years.Methods Patent Database of State Intelletual Property Office was retrieved by IPC class numbers combined with keywords and the IPC classification of the Chinese medicine patents in Shanghai area during 2002-2013 were under statistical analysis.Results The hot spot distribution of therapeutic activity of TCM preparations:Antineoplastic is the most important activity of Chinese medicine development, followed by the activities of treatment of inadequate blood flow or cardiovascular system diseases, such as hyperglycemia, high cholesterol, and so on. The activity of treatment of the digestive tract or digestive system diseases, followed by the activities of anti infection, antiviral, antibacterial, anti-inflammatory treatment, etc. The hot spot distribution of dosage:Researches on new dosage of Chinese medicine were mainly reflected in the improvement of traditional dosage form and development of new formulations. The capsules, tablets, oral liquid, and injections were the new dosage forms under the most researches. The distribution of medical resources:Medicinal plants or animals mainly came from “angiosperm”. Medicinal animals were less than medicinal plants, which mainly belonged to insecta.ConclusionThe hot spots of innovative technology of Chinese medicine industry in Shanghai are mainly distributed in the medicine of anti-tumor, cardiocerebral vascular diseases, digestive system diseases and so on in recent years. The researches of new formulation focus on the traditional dosage form improvement and new formulations development. “Angiosperm” is the key to the development of medicinal plants.

BACKGROUND:At present, the study to balance ability of functional ankle instability patients is less. The
balance ability deficiency may also be one of the important reasons for ankle repeated injury, so the research can effectively reduce the damage probability.
OBJECTIVE:To explore the static balance ability characteristics of functional ankle instability patients.
METHODS:Ten male subjects with unilateral functional ankle instability were enrol ed as the experimental group in the study, and other 10 healthy match-paired subjects were served as the control group. Their static balance ability was measured under double feet standing and single foot standing using win-pod system, the double feet testing lasted for 30 seconds and the single foot testing lasted for 10 seconds. Evaluation parameters of balance ability were obtained.
RESULTS AND CONCLUSION:During double feet testing, there were no significant differences in balance
ability parameters between the experimental group and the control group whether eyes be opened or closed (P>0.05), but the index values of double feet testing were obviously less than those of single foot testing. So we
concluded that in the double feet standing, there was no significant difference in the static balance ability between functional ankle instability patients and normal persons. Moreover, the static balance ability of double feet was
better than that of single feet. In the single foot standing of the affected foot, the static balance ability of functional ankle instability patients was slightly worse than that of the normal persons, especial y with eyes closed.
Comparison of the average swing between the X-axis and Y-axis found that in the process of maintaining balance posture, the swing direction of the body center of gravity was mainly in the anteroposterior direction.

BACKGROUND: The coating induced by conventional simulated body fluid (SBF) and 1.5-folds SBF as biomimetic method has the shortcomings of slowly growth with weeks or months growth cycle. OBJECTIVE: To evaluate Ti-6Al-4V alloy surface deposition of hydroxyapatite coating induced by 3-folds SBF. DESIGN, TIME AND SETTING: In vitro biological analysis. The experiment was performed at the Institute of Metal Research, Chinese Academy of Sciences from November 2008 to March 2009. MATERIALS: Ti-6A1-4V alloy was supported by Boda Metel Materials Co., Ltd. METHODS: The pretreated Ti-6Al-4V alloy was immersed in 37 'C 3-folds SBF. The SBF was replaced every 24 hours for 3 days. MAIN OUTCOME MEASURES: The morphology, phase and elemental composition of calcium phosphate coating were analyzed by scanning electron microscopy, X-ray diffraction and infrared spectroscopy analysis. RESULTS: The scanning electron microscopy exhibited that the surface of Ti-6A1 -4V alloy was covered with deposited film with granular crystal on local areas at day 1 after immersion. The deposited film covered surface of Ti-6A1-4V alloy at day 3 after immersion, and granular or scale-shaped crystal could be seen. The crystal deposition displayed sustained growth with certain area as center. The product comprised Ca, P, O, C elements without Al, V. X-ray diffraction and infrared spectroscopy analysis revealed that the main component of the coating was hydroxyapatite. CONCLUSION: The 3-folds SBF can accelerate the deposition as well as shorten formation duration of hydroxyapatite coating on Ti-6A1-4V alloy.

Objective To explore whether the inhibited expression of fibrocystin by RNA interference can increase epidermal growth factor (EGF)-induced cell proliferation and its possible mechanism . Methods A stable PKHD1-silenced HEK 293 cell line was established . Cell proliferation rate, intracellular Ca2+ concentration and extracellular signal-reguhted kinase 1/2(ERK1/2) activity were assessed after treatment with EGF, verapamil and Bay K8644 . Results The proliferation rate of PKHD1-silenced HEK-293 cells was found to be significantly higher after EGF stimulation compared to the control HEK 293 cell (231 .5% vs 152 .8%, P＜0 .01) . PKHD1-silencing lowered the intracellular Ca2+ concentration and caused EGF-induced ERK1/2 overactivation in the cells(P＜0 .01 ) . When cells were treated with verapamil for 4 hours to lower the intracellular Ca2+ concentration, the cell proliferation rate was significantly increased after 20 ng EGF for 24 hours . The verapamil treatment increased the level of activated ERK1/2 in EGF-treated cells . An increase of intracellular Ca2 + in PKHD1-silenced ceils repressed the EGF-dependent ERK1/2 activation and the hyperproliferative response to EGF stimulation . Conclusions Inhibition of fibrocystin can cause EGF-induced excessive proliferation through decreasing intracellular Ca2+ resulting in EGF-induced ERK1/2 activation . The loss of fibrocystin may lead to abnormal proliferation in kidney epithelial cells and cyst formation in ARPKD through modulation of intracellular Ca2+ concentration .

From the soon-to-be-completed Human Genome Project to Combinatorial Chemistry and High-Throughput Screening combined with bioinformatic tools, scie n tific advances are poised to revolutionize drug discovery and even health care. The research directions in the State Key Laboratory of Natural and Biomimetic Dr ugs of Peking University mainly focus on the study of interaction between biolog ical targets and active compounds with structural diversity including endogeno us and exogenous substances, especially the natural products that come from anim als, plants and inorganic medicines. The new lead compounds have been designed and s ynthesized based on the structure-activity relationship and computer modeling. T he research activities are involved in 76 national research projects. From 1996 -20 00, 310 papers have been published, 8 patents obtained an d 14 prizes of scientific achievements awarded by the Chinese government and min istries.

Objective.To observation the change of thyrohyoid muscle(THM)after recurrent laryngeal nerve(RLN)has been cutting off.The animal experimental results were reported.Method.The animal experimental model of RLN paralytic was completed in 9 dogs.The specimens of THM were treated by histochemiscal staining after 2,4,6,8,10,20 day operation respectively and the diameter and areas of three types muscle fibers were determined by microscope.Results.The diameters and areas about 3 types muscle fibers of THM descended after operation,the value of them reached the minimum simutaneously,then recovered step by step.Conclusion.The changes of THM was related to operative trauma and nutrition disorder probably,while it was not related to RLN cutting.

Objective:To study histocompatibility of self-made stents made of NiTi alloy in rabbits.Methods: To place 30 stents made of NiTi alloy into 30 rabbits trachea through mouth under general anethesia,to observe local tissue reaction from 1 week to 6 months and to try to find pathological changes of main organs of rabbits in the 6 th month. Results:During observation,apart from dyspnea in two rabbits,one died at 3 day and the other died at 6 week after implantation. Autopsy showed the implanta.tion sites were occluded by granulation tissue. All other rabbits were normal and implantation sites were patent. In the first three weeks we could observe acute inflammation at the implantation sites while after 5 weeks we could observe chronic inflammation there. We observed that the structure of epithelial cells tended to be normal by telemicroscope. There are no pathological changes in liver, spleen, kidney, lung, heart and brain tissue. Conclusion:Self-expanding stents possess good histocompatibility and have no toxic reaction to whole body and fit to implantation into human body.

Objective To explore the changes of the survival quality in stomach cancer patients before and after surgery and its influencing factors.Methods The patoents with stomach cancer have been diagnosed in 202 cases,through the QLQ-C30 scale before another opponent live quality investigation,as well as many variables (conditional Logistic regression) method,the life quality of correlation factors of gastric cancer patients were anelyzed.Results Compared with before treatment,after a month QLQ-C30 various quality of life index compared to baseline state basic recovery,the difference was not statistically significant ( P ＞ 0.05 ),and a week after QLQ-C30 various quality of life index and before treatment is statistically significant ( all P ＜ 0.05 ).Conclusion Gastric cancer patients before and after surgery survival influence factors on the quality of the main factors for the population sociology characteristics,social support,male,operation methods and medical expenses,should strengthen the psychological and social function of patients to improve.

Glycohemoglobin (HbA1c) is an important indicator for assessing long-term glycemic levels and diabetic complications of blood vessels in diabetic patients.However the test methods,reagents and technological capabilities in different laboratories are various,so there are significantly differences of the results in bias.In 2010 HbA1c was included in new guidelines from ADA for diagnosing diabetes,and recommended by WHO in 2011,which promoted the standardization process of HbA1c tests.In this article we analysed and reviewed main detection methods,the international advancement of standardization,current status of the establishment of HbA1c Reference system in China and its clinical application.

Objective To evaluate prognostic effects and complications of interventional embolization at different time points on patients with ruptured intracranial aneurysms of Hunt -Hess Ⅳ-Ⅴlevels.Methods Through a retrospective analysis from 2008.1 to 2012.12, we collected 162 from 200 patients who underwent interventional embolization due to ruptured intracranial aneurysms according to the criteria.Patients with serious neurological disorders , coma, and end-stage diseases were excluded.Brain CT scan was performed to identify the spontaneous subarachnoid hemorrhage of the patients , followed by CTA or digital subtraction angiography ( DSA) to ensure the accurate diagnosis of intracranial aneurysms.All of the patients at Hunt-HessⅣ-Ⅴlevels were treated with interventional embolization , and were divided into two study groups:early stage therapy group of 79 patients ( embolization at the time <72 hours after the onset) and late stage therapy group of 83 patients ( embolization at the time ≥72 hours after the onset ).Factors including pre-and post-interventional Glasgow coma score ( GCS ) and Glasgow outcome score , ( GOS) as well as barthel index ( BI ) were statistically compared between the study groups.T-test was performed to compare the data between the two study groups.Chi-square test was taken to evaluate the enumeration data.And the correlation of therapeutic time and prognosis of patients was demonstrated by binary regression analysis.Results In the patients with ruptured intracranial aneurysms at Hunt-Hess Ⅳ-Ⅴlevels, early stage intervention (63.5 ±13.5) resulted in significantly better BI after three months , and demonstrated better recovery in comparison to the late stage intervention group (34.8 ±12.7) ( t=6.127, P<0.05).But no statistical significance of post-operative GCS (6.6 ±1.6 in early therapy group;6.1 ± 1.4 in late therapy group ) and discharging GOS ( 3.0 ±0.7 in early therapy group; 2.9 ±0.7 in late therapy group) was found between the two groups (t=1.822, 0.631 respectively; P>0.05).The early therapy group also presented a significant decrease of complications , such as hydrocephalus (χ2 =6.369, P<0.05 ) and venous thrombosis (χ2 =5.158, P <0.05 ), compared to the late therapy group.Furthermore, Statistical analysis revealed that therapeutic time closely correlated with the prognosis of patients with ruptured intracranial aneurysms at Hunt-Hess Ⅳ-Ⅴ levels ( P =0.009, OR =3.897).Conclusion The early stage therapy can improve the prognosis and decrease incidence of complications , which should benefit the efficient management of the patients with interventional embolization of ruptured intracranial aneurysms.