Objective To investigate the effects of loop electrical excision procedure(LEEP)on pregnancy of patients with cervical epithelial neoplasia(CIN).Methods 83 patients with cervical intraepithelial neoplasia grade II~III have been diagnosed.Cervical loop trip were 38 cases for all of the observation group,did not undergo cervical loop were 45 cases for all the control group.observed pregnancy.Results There are significant differences in premature delivery between two groups(P0.05).Conclusion Reasonable 1oop electrical excision procedure will not increase the risk of pregnancy.

Objective:To explore the approach to analyzing simple effect on factorial design with SAS.Methods:The mean square of error coming from factorial design was considered as standard error(SE),and simple effect analysis under Contrast statement in GLM was conducted by designing the contrast coefficient matrix of the parameter,and SAS program was realized based on an example of 3-way factorial design.Results:he procedures of designing the contrast coefficient matrix of the pa-rameter were achieved.Conclusion:It is convenient and feasible to conduct simple effect analysis under Contrast statement by constructing design matrix.

Objective To investigate mRNA expression levels of cathepsin B,cathepsin L and uPA in non-Hodgkin lymphoma (NHL) samples,and analyze the relationship between three genes and clinicopathological factors.Methods Real-time fluorescence quantitative RT-PCR was used to quantify the relative expression levels of cathepsin B,cathepsin L and uPA mRNA in 39 NHL patients without chemotherapy or radiotherapy and 15 patients with lymph node reactive hyperplasia.Relationship between their expression and clinico-pathological factors was analyzed.Results mRNA expression levels of cathepsin B,cathepsin L and uPA in NHL group were significantly higher than those in the control group (0.403 vs 0.128,0.209 vs 0.057,0.459 vs 0.031,all P ＜ 0.05).The mRNA expression levels of cathepsin B,cathepsin L and uPA were correlated with each other in NHL.The mRNA expression levels of cathepsin B,cathepsin L and uPA in advanced staged NHL were markedly higher than those in early staged NHL (0.453 vs 0.341,0.204 vs 0.085,0.372 vs 0.196,all P ＜ 0.05).mRNA levels of cathepsin B,cathepsin L and uPA were higher in NHL patients sensitive to chemotherapy than in those resistant to chemotherapy(0.401 vs 0.556,0.085 vs 0.205,0.316 vs 0.499,all P ＜ 0.05).Conclusions The high expression of cathepsin B,cathepsin L and uPA could be detected in NHL,and their expression has a close relationship with the biological behavior characters of NHL.The dynamic examination of cathepsin B,cathepsin L and uPA may be used as clinical auxiliary diagnosis and prognosis of NHL.

Objective To investigate whether efflux mechanism is involved in azole resistant Candida albicans strains isolated in China. Methods We compared rhodamine 6G uptake and glucose induced efflux between azole sensitive and azole resistant strains to elucidate resistance to azole due to efflux pump, then measured mRNA level of efflux gene cdr1 and cdr2 by reverse transcriptase polymerase chain reaction (RT PCR). Results Rhodamine 6G moved from extra into the intracellular compartment in both azole resistant and azole sensitive strains quickly when in glucose free incubation condition. However, efflux of rhodanmine 6G was enhanced significantly in azole resistant strains and decreased in azole sensitive strains while adding glucose in the media. The mRNA level of efflux gene cdr1 and cdr2 was higher in clinical azole resistant strains and fluconazole induced resistant strains than strains reverted from resistance to sensitivity. However, all such strains had higher efflux gene expression than azole sensitive strains. Conclusions Efflux mechanism is associated with the resistance to azole in Candida albicans strains isolated in China. Fluconazole can induce efflux pumps expression leading to resistance to azole but the resistance to azoles can be reversed after withdrawing fluconazole.

MicroRNA is a type of endogenous noncoding small molecular RNA with a length of 19-25 nucleotides. MicroRNAs are involved in almost all key biological processes, such as cell proliferation, differentiation, apoptosis, and hematopoiesis, via repres-sion of target gene expression. Acute lymphoblastic leukemia (ALL) is the most common type of pediatric cancer. It is a heterogeneous disease with different clinical signs, morphology, immunophenotype, and cytogenetics. Currently, several microRNAs have implicated aberrant expression in ALL and play an important role in cancer initiation and development. MicroRNA expression pattern is associated with clinical feature, prognosis, and treatment. Therefore, microRNAs could act as a novel marker to help scientists learn about the pathogenesis of ALL and to provide more effective treatment options.

Heart disease is the major disease that threaten human health. ECG is an important tool for the diagnosis of cardiac disease. As traditional ECG measurement devices have many disadvantages, a non-contact ECG measurement system was designed. With the non-contact electrode based on capacitive coupling, the signals were collected and then they were amplified and filtered. The conditioned analog signal was converted to digital data which was sent to the mobile terminal through bluetooth. Finally, the ECG data was analyzed to extract the key ECG parameters. The results showed that the precise ECG signals can be got with the non-contact electrode and the key ECG parameters can be acquired accurately.
Electrocardiography, Ambulatory ; instrumentation ; Electrodes ; Equipment Design ; Signal Processing, Computer-Assisted

Objective To investigate the effect of comb interferon( comb interferon CITF) uniting virazole to cure recurring or non-responding chronic hepatitis C.Methods Hypodermic comb "interferon 15μg,3 times every week,subcutaneously injected,24 weeks course of treatment was adminstered to 74 patients of recurring or non-responding chronic hepatitis C.Clinical symptom,physical sign,ALT,hepatovirus continual response rate and the condition of adverse effect were observed during treatment,chron-culminate,follow to 6 months' withdrawl.Results 72 patients accomplished treatment,HCV-RNA of recurring group and non-responding group to continual negative change rate were 73% and 37% (P＜0.05)after treatment termination,without severity adverse reaction being found.Condusion Comb interferon 15 μg/d combining virazole 900mg/d have better curative effect in curing recurring or nonresponding chronic hepatitis C.

Objective To explore clinical effect and life quality study of Shenyi capsule combined with docetaxel and cisplatin in treatment of advanced breast cancer.Methods 135 cases of advanced breast cancer of Huangshi Central Hospital were collected and diagnosed from July 2013 to December 2014 in accordance with the random number table,were divided into treatment group(n=69) and control group(n=66).Both groups were given regular basis treatment, control group was treated by docetaxel(75 mg/m2,twice a day)and cisplatin(25 mg/m2,once a day), and treatment group combined with Shenyi capsule(20 mg,twice a day)on the basis of control group treatment.4 weeks were 1 course, and all the cases were taken 12 weeks of continuous treatment.Observed and compared the clinical efficiency and life quality of two groups.Results The remission rate of 63.77% in treatment group was higher than control group of 40.91%, difference was statistically significant(χ2 =7.070,P=0.008).After treatment, the SF-36 scale in treatment group significantly increased from ( 54.69 ±8.78 ) to ( 63.87 ±12.08 ) , and higher than that of control group ( 57.95 ±11.95 ) , differences were statistically significant(P<0.05).The adverse reactions in treatment group was lower than control group, difference was statistically significant(Z=2.858,P =0.000).Conclusion The clinical effect in treatment of advanced breast canceris obvious by Shenyi capsule combined docetaxel and cisplatin.It can significantly improve clinical effect and life quality.

Objective To investigate the effect of HgCl2 and drugs on the expression of calcitonin gene related peptide(CGRP) in the spinal cord and dorsal root ganglion(DRG).Methods Forty male SD rats(180-220 g) were randomly divided into 7 groups,5 in each treated group,10 in the control.Group A(high HgCl2),B(high HgCl2 +DMPS),C(high HgCl2+DMPS+ carbamzepine) were administrated with HgCl2 at the dose of 17 mg/kg(1/4 LD50),group D(low HgCl2),E(low HgCl2+DMPS),F(low HgCl2+NS) at the dose of 8.5 mg/kg(1/8 LD50),by gavage,once a day,the control group treated with normal saline(NS) in the same way.The sub-acute mercury poisoning models were developed within 18-22 days.Then five rats of control group and group A and D were killed and group B and E were treated with the chelator(DMPS) by intraperitoneal injection at the dose of 28 mg/kg for 2 periods.Group C was given DMPS+ carbamzepine and group F was given NS only.The carbamzepine was used at the dose of 16.82 mg/kg by gavage daily for 14 days.The spinal cord(L5-6) and the DRG of L5 and L6 were collected after the treatment finished.The immunohistochemistry SABC way was used to detect CGRP.Results The CGRP level in spinal cord and DRG of the rats exposed to HgCl2 increased significantly compared with the control group(P

The activities of acid phosphatase and ?-1,4 glucosidase and the concentration of citric acid in the semen of 51 cases of chronic prostatitis were determined.The mean value was 694.33?346.61 Bodansky units for acid phosphatase,43.84?24.70mIU/ml for ?-1,4 glucosidase,and 3.26 ?2.37 mg/ml for citric acid.The activity of acid phospohatase and the concentration of citric acid were significantly lower in the patients with chronic prostatitis than in the normal subjicts while the activity of ?-1,4 glucosidase showed little changes.The findings suggest that the functions of the prostate was severely imparired and that of the epididymis remained normal in patients with chronic prostatitis.