Humans ; Silicosis ; prevention & control

ObjectiveTo explore the feasibility and safety of the spleen-preserving surgery in the treatment of traumatic rupture.Methods47 patients with traumatic rupture were treated with spleen-preserving surgery:the biological glue which local physical bleeding had 7 cases,suture repair had 13 cases,splenic artery ligation had 10 cases,splenic artery ligation and splenic repair had 9 cases,partial splenectomy had 8 cases.Results47 patients had four cases of infection,and cured after dressing,1 case had postoperative bleeding symptoms and used splenectomy surgery.46 patients with spleen-preserving operation is successful,the average length of stay was 15 days,there is no deaths.After 6 months,all patients used abdominal B-utrasoundor CT scans to confirm the survival of spleen is good and indicator of the examination is normal.Follow-up of these patients had no dangerous infections and complications.ConclusionSpleen-preserving surgery in the treatment of traumatic rupture was safe,feasible,could retain the greatest degree of function,and could be used in clinical practice.

China ; Humans ; Occupational Health ; Occupational Medicine ; Research

Apoptosis ; drug effects ; Asbestos ; toxicity ; Humans ; Mineral Fibers ; toxicity

Besides 36 (28 single-tablets and 8 fixed-dose combinations) used antiretroviral drugs clinically, there are a number of investigational antiretroviral agents currently in phase 2-3 clinical trial. Tenofoviralafenamidefumarate (TAF) is a novel nucleoside analogue reverse transcriptase inhibitor that is potent and less toxicity than tenofovir (TDF). Doravirine is a non-nucleoside analogue reverse transcriptase inhibitor that demonstrates activity against NNRTI-resistant viral strains. GSK744 is an integrase inhibitor with a long acting preparation. In addition, several drugs with new mechanisms are also noted, for example, BMS-663 068 is a small molecule CD4 attachment inhibitors and cenicriviroc is a novel CCR5/CCR2 antagonist with antiretroviral activity and anti-inflammatory effects. Several drug classes that target known pathways in HIV latency have being developed, and leading the list are histone deacetylase inhibitors. Other agents include protein kinase C activators, positive transcription elongation factor activators, DNA methyl-transferase inhibitors and histone methyl-transferase inhibitors and so on. This review is focused on the above-mentioned drug candidates that may be used in clinical in next couple of years and those compounds that can reverse latent HIV infections.
Adenine ; analogs & derivatives ; therapeutic use ; Anti-HIV Agents ; therapeutic use ; HIV Infections ; drug therapy ; Humans ; Organophosphates ; therapeutic use ; Organophosphonates ; therapeutic use ; Piperazines ; therapeutic use ; Pyridones ; therapeutic use ; Reverse Transcriptase Inhibitors ; therapeutic use ; Tenofovir

Objective To study the biological effects of hirudin on the cell circle of fibroblasts in hypertrophic scar.Methods Fibroblasts were taken from normal skin and hypertrophic scar,and cultured in vitro with various concentrations of hirudin.Then,flow cytometry was used for observing cellcycles,and Western blot for some proteins (p27 and cyclin E) related with cell circle.Results With the increasing of the concentrations of hirudin,fibroblasts on G1 phase increased and on S phase decreased,and the expression of protein p27 increased,but that of protein cyclin E decreased.Conclusion Hirudin can influence the cell circle of fibroblasts derived from normal skin and hypertrophic scar by means of the expression of some proteins related with celI circle.Hirudin causes G1 phase arrest and inhibits the production of fibroblasts.Therefore,it seems that hypertrophic scar could be prevented and treated with hirudin.

Objective To establish a simple and rapid method for determination of trace chromium(Ⅵ) in drinking water. Methods In the weak acid solution containing n-butyl alchhol,trace chromium (Ⅵ) in the water connected with diphenylcarbazide and sodium dodecyl sulfate into purplish red ternary iron compound,which could be extracted by chloroform and quantified at wavelength of 550 nm. Results The detection limit was 0.001 mg/L,the RSDs were 1.1%-1.4% and the recovery rate was 96%-113%. Conclusion The method was rapid,simple and the sensitivity and accuracy will meet the requirement of standard test methods for drinking water.

Objective To evaluate the effect of conditioned medium made up of ConA-activated spleen cells supernatant on cultured rabbit corneal endothelial cells (RCECs).Methods RCECs were primarily cultured in vitro by revealing descemet membrane and endothelium combining tissues masses.Culture medium was RPMI 1640 with 10% FBS and varied concentrations of conA-activated spleen cells supernatant.Experimental groups were divided by different concentrations of ConA-conditioned supernatant:5%,10%,15%,and 20%.In the control group only RPMI1640 and 10% FBS were used.Methyl thiazolyl tetrazolium colorimetry analysis was used to evaluate the proliferation of RCECs cellular population.The cultured cells were identified by immunohistochemical staining for neuronal specific enolase (NSE).Results The RCECs were cultured successfully.RCECs presented positive expression in cytoplasm with NSE immunohistochemical assay.There were significant differences in the experimental groups compared with the control group (P

Objective To investigate the effects of adiponectin on cell activity and expression of T-cadherin in human retinal pigment epithelial(hRPE) cells cultured with high glucose.Methods ①The hRPE cells were divided into three groups:normal glucose group(5.5mmol/L glucose),high glucose group (33mmol/L glucose),high glucose plus adiponectin group (33mmol/L glucose+2.5ug/ml adiponectin).48h later,the cell activity was observed by MTT assay.②After the above groups were cultured respectively for 24h,48h,72h again,the expression of T-cadherin mRNA was observed by RT-PCR. Results ①Cell activity in high glucose group significantly decreased. Cell activity adiponectin group was higher than that in the high glucose group.②With the extension of time ,the expression of T-cadherin decreased in high glucose group,but increased in adiponectin group. Conclusion Adiponectin may be protect the hRPE cells by T-cadherin.

Objective To study the expression of Livin and its relationship with Caspase-3, FHIT in skin squamous cell carcinoma. Methods lmmunohistochemical assay was used to detect the expression of Livin, Caspase-3 and FHIT in 48 cases of sSCC, 20 cases of chronic ulcer and 12 cases of normal skin tissues. Results The rate of positive expression of Livin in SCC tissues was significantly higher than that in the chronic ulcer tissues and normal skin tissues (P <0.01). The rate of positive expression of Caspase-3 and FHIT in SCC tissues was significantly lower than that in the chronic ulcer tissues and normal skin tissues (P < 0.01).The expression of Livin and Caspase-3 had relationship with the lymphatic metastasis and histologic differentiation of SCC(P <0.05). The expression of Livin and Caspase-3 had not relationship with age, sex, the sizes of SCC (P >0.05). There was negative significant correlation between the expression of Livin and the expression of Caspase-3, FHIT. Conclusion There may be relationship between the abnormal expression of Livin and Caspase-3, FHIT and the occurrence, progression of skin squamous cell carcinomas. They may become a new method for preservation and treatment skin of squamous cell carcinomas.