Objective A clinical trial of intradiscal injection of hypertonic saline to treat lumbar disc herniation, and observation of its effect, complications and changes in intervertebral disc tissue were performed for clinical application. Methods Discography was performed under local anesthesia, followed by a single injection of 1.0～2.5 ml 10% hypertonic saline. Results 262 cases were included in the study, with 4 years of average follow up. The final outcome ranked excellent in 93 cases and good in 100 cases. As a whole, the effective rate was 75.7% and the improvement rate was 71.5%. The procedure was effective in protrusion and confined extrusion types. 13 cases (6.7%) relapsed after the injection. No complication occurred. 26 cases resorted to surgery because of failure in intradiscal therapy, but no change was found, in the nerve and surrounding tissue of the intervertebral disc attributable to the injection during the surgical operation. The main causes of failure were the marked protrusion or non-contained extrusion, or the combination with spinal canal stenosis. Changes in the nucleus pulposus shown microscopically were similar to those resulted by the action of enzyme injection, but the injury to the disc was much milder.Conclusions Intradiscal therapy with 10% hypertonic saline for lumbar disc herniation is effective, mini-traumatic and safe.

Objective The levels of endothelin-1(ET-1) and calcitonin gene-related peptide(CGRP) in the plasma and cerebrospinal fluid(CSF)after acute cervical spinal cord injury. Method ET-1 and CGRP were determined by RIA in order to observe the effects of naloxone on levels of ET-1 and CGRP and neuronal death of cervical spinal cord. Result Levels of ET-1 in the plasma and cerebrospinal fluid(CSF)after acute cerical spinal cord injury increased significantly; but the levels of CGRP in the plasma and cerebrospinal fluid(CSF) decreased markedly. The levels of ET-1 in the plasma and cerebropinal fluid(CSF)decreased markedly after the threapy of naloxone; but levels of CGRP in the plama and cerebrospinal fluid(CSF)increased sinificantly. Conclusion These deta suggest that the lessening of CGRP and increasing of ET-1 may be some important reson causing death of neuron after acute cervical spinal cord injury, and naloxone can protecet cervical spinal cord neuron by increasing levels of CGRP and decreasing levels of ET-1.

Objective To promote the level of morphological diagnosis and estimate prognosis combining the result of bone marrow histotomy and the swear of 82 cases of malignant hemology disease. Methods We collected 82 cases of malignant hemology disease, extracted marrow by one step and routinly made bone marrow swear and dyeing, made histotomy by embedding plastic. Diagnosis of malignant hemology disease refered to the standard of WHO 2001. Results Bone marrow histotomy was superior to bone marrow swear in diagnosing MF and hypocellular MDS. Bone marrow histotomy was easier to find cell of lymphoma than bone marrow swear. Bone marrow histotomy was a more sensitive index of tumor load of MM.It was also helpful for judging acute change of CML and relapse of acute leukemia. Conclusion Level of diagnosing malignant hemology disease can be promoted efficiently by combining marrow histotomy with bone marrow swear

Objective:To study the clinical efficacy and safety of BCG-polysaccharide nucleic acid combined with isotretinoin soft capsules in the treatment of plane warts. Methods:Seventy patients with plane warts were randomly divided into the observation group and the control group(n=35). The control group were treated with isotretinoin soft capsules, limp 10mg, bid, 4 weeks to 10mg, qd. The observation group were given BCG-polysaccharide nucleic acid 1ml, im, every otherday, on the basis of the contrl group. After 8 weeks of treatment, T cell populations and immunoglobulin levels were detected, and the efficacy and adverse reactions were recorded. Results:After the treatment, T cell population level, IgG and IgA of the two groups were significantly improved(P<0. 01), and the improvement in the observation group was significantly better than that in the control group (P<0. 05). The onset time of the observa-tion group was shorter than that of the control group ( P<0. 01). The total effective rate of the observation group was higher than, that of the control group(P<0. 05). The occurrence of adverse reactions in the two groups were no significant(P>0. 05). Conclusion:The clinical efficacy of BCG-polysaccharide nucleic acid combined with isotretinoin soft capsules in the treatment of plane warts is sig-nificant with promising security.

OBJECTIVE:To study the characteristics of adverse drug reactions(ADR) induced by gatifloxacin.METHODS:A total of 866 ADR cases induced by gatifloxacin collected in Zhejiang ADR Monitoring Center between Oct.30,2003 and Oct.30,2006 were analyzed with Excel.RESULTS:The common ADR induced by gatifloxacin were lesions of skin and appendages,gastro-intestinal system,systemic and nervous system.Most of the cases were slight in symptoms,but gatifloxacin could also induce severe ADR such as dysglycemia etc that resulting in potential risks to vital human organs.CONCLUSION:ADR monitoring should be emphasized when using gatifloxacin so as to decrease or avoid the occurrence of ADR.

The elderly in the community who is the focus of the family doctor services , because of his special physical and mental condition , their demands for health care services showing the features of dependence , Recur-rence and urgency .Therefore, as the provided entity of community health service , the family doctor should care for the elderly in the community .In order to promote the realization of Healthy aging , we can build the system of the humane care for the elderly in the community , through the education and guidance of health、conducting psychologi-cal counseling , providing emotional support、building family sickbeds , providing regular visits、emphasizing clinical narrative, establishing mutual participation model and other measures .

Objective To compare the different expression patterns of survivin and caspase-3 between the larger part and the smaller part of the dissymmetric enlarged prostates in BPH patients, and to clarify the role of apoptosis in BPH etiology. Methods BPH tissues from 25 patients were embedded in paraffin. The specimens constituted of two prostate parts from each patient. One part was from the larger enlarged gland and the other was from the smaller enlarged counterpart. 50 paraf-fin blocks were cut into sections and stained with survivin and caspase-3 polyclonal antibodies against human. All the data was analyzed by SPSS13.0 software. Results Survivin immunoreactivity was localized in prostatic cell nuclei. Survivin expression in larger enlarged parts (22.08±16. 33)0% was significantly higher than the smaller parts (7. 645±5. 45)%(P<0.01). Positive nuclear or cytoplasm staining was also observed in the Caspase-3 immunoreactivity. Caspase-3 expression was higher in smaller enlarged parts (51. 525±27. 27%) than in larger enlarged parts (33. 125±21.01%) (P< 0.01). Survivin expression in larger part of the enlarged prostates was inversely correlated with caspase-3 expression (r=-0. 474, P<0. 05). However, in smaller part of the enlarged prostates, the expression of the two factors had no relation (r=-0. 313,P>0. 05). Conclusions Survivin and caspase-3 expression patterns are different in the two parts of the dissymmetric enlarged prostates. These different patterns indicate apoptosis playing an important role in BPH development.

OBJECTIVE The short-term efficacy of endoscopic adenoid hypothermic plasma ablation and its effect on Eustachian tube function. METHODS 75 patients with obstructive sleep apnea hypopnea syndrome who were treated in our hospital from March 2013 to March 2015 were divided into two groups according to the different operative methods: nasal power cutting group(30 cases) and low temperature plasma ablation group(45 cases), comparison of the two groups of patients with surgery time, intraoperative blood loss and follow-up after 6 months of clinical efficacy and Eustachian tube function. RESULTS The time of resection, operation time and intraoperative blood loss were significantly better in the low-temperature plasma ablation group than that in the rhinectomy group, the difference was statistically significant(P<0.05); The incidence of complications in the low-temperature plasma ablation group was significantly higher than that in the rhinectomy group, and the difference was significant(P<0.05); there was no significant difference between the two groups in the total effective rate and the postoperative disease-specific quality of life questionnaire(OSA-18) and there was no significant difference in the quality of life between the two groups(P>0.05). CONCLUSION The clinical efficacy of low-temperature plasma ablation is close to nasal dynamic cutting, but low-temperature plasma ablation surgery in the amount of bleeding was significantly less than the nasal excision. This surgical approach is minimally invasive and safe, but its postoperative secondary infection and secondary bleeding is high, so this need for further study.

To investigate the expression of mRNA and protein of telomerase reverse transcriptase(TERT) in guinea pig asthmatic lung tissues and human circulating lymphocytes and to discuss the role of TERT in the pathogenesis of asthma and the clinical significance of expression of TERT in human asthmatic circulating lymphocytes in monitoring the state of asthma.Methods: In 6 guinea pig asthmatic lung tissues, 6 guinea pig normal lung tissues, 16 samples of circulating lymphocytes of asthmatic patients and 14 normal circulating lymphocytes the expression of mRNA and protein of TERT were detected with nuclei acid in situ hybridization and immunostaining. The expression of TERT in circulating lymphocyte were compared with pulmonary function and serum IgE level. Results:The expression of mRNA and protein were up-regulated in asthmatic airway epithelium and lymph nodule germinal center in guinea pig. Compared with control group,high expression were also showed in human asthmatic circulating lymphocytes.The index of TERT expressive positive lymphocytes showed negative correlation with FEV1/ predictive value and MW/predicn've value,positive correlation with serum IgE level. Conclusion: (1 )TERT expression in asthmatic airway epithelium and lymph nodule germinal centers of guinea pig and human circulating lymphocytes were up-regulated; (2)Detection of TERT expression of asthmatic circulating lymphocytes may be useful to monitor the state of asthma.

After the necessity of using medical news information and the advances in its automatic indexing were analyzed, a novel automatic controlled indexing method of medical news text was put forward. The method intro-duced translated MeSH vocabulary as the main indexing words, merging Chinese commonly used word segmentation dictionary, then calculated word frequency for document text which added split token and sorted it, choose top 5 high-frequency words in MeSH vocabulary indexed document after deleting high-frequency words not in MeSH vo-cabulary.