For the purpose of creating a hospital culture, the present paper summarizes theories on research-oriented organizations, addresses the significance of developing a research-oriented hospital, summarizes the means and methods for developing a research-oriented hospital according to the model of a research-oriented organization, and demonstrates the effects of a research-oriented hospital and promoting functions for hospital culture.

Pancreatic cancer is a common malignant neoplasm of digestive system with a fairly poor prognosis, the diagnosis rate in its early stage is low, and in recent years its incidence rate has become more and more high. Surgical remove is the only radical means, whereas only 20% of patients can get the oppotunity of surgery when the diagnosis is confirmed. More unfortunately, the five-year overall survival rate after the surgery is as low as 15 - 20%. Therefore, it is of clinical significance to improve the survival rate and to prolong survival time. Comprehensive interventional therapy has many advantages, as the technique is safe and mini-invasive with satisfactory short-term efficacy. Nowadays, interventional therapy has become the hot point in the research of pancreatic cancer. This paper aims to make a review about the current situation and progress of interventional treatment for advanced pancreatic cancer.

OBJECTIVE: To explore the mechanism of Kechuanluo oral liquid, a compound Chinese herbal medicine, in inhibiting allergic airway inflammation by observing the effects of Kechuanluo on eosinophil (EOS) apoptosis and its regulation factors in asthmatic mice. METHODS: Fifty-six BALB/c mice were randomly divided into normal control group (n=16), untreated group (n=16), Western medicine group (n=12) and Kechuanluo group (n=12). Except for the mice in normal control group, asthma was induced in BALB/c mice by using ovalbumin (OVA) and potassium aluminium sulfate. The mice were intragastrically administered with normal saline, Kechuanluo (30 ml/kg daily) and prednisolone tablets (10 mg/kg daily) respectively for two weeks. At 0 hour, the 3rd, 7th and 14th day after the end of OVA sensitization, the EOSs of lung tissues were counted by improved-Giemsa staining method; immunohistochemical method and image analysis were used to detect the expressions of Fas, FasL and Bcl-XL in the EOSs in the four groups; and apoptotic rates of the EOSs in the lung tissues of asthmatic mice were detected by terminal deoxynucleotidyl transferase-mediated biotin-dUTP nick end labeling (TUNEL) technique. RESULTS: Compared with the untreated group, airway inflammations of the mice in the Kechualuo group and Western medicine group were lessened and the EOS counts decreased on the 3rd, 7th and 14th day after the last OVA sensitization (P<0.05). On the 3rd day, the EOSs apoptotic rates, the expression areas of Fas in the EOSs and FasL in the lung tissues were significantly higher in the Kechuanluo group than those in the untreated group (P<0.01), furthermore, the EOS apoptotic rate reached the peak level. Inversely, the EOS count and the expression area of Bcl-XL in the EOSs were obviously lower in the Kechuanluo group (P<0.05). On the 7th and 14th day, the expression areas of Fas and Bcl-XL in the EOSs were significantly decreased in the Kechuanluo group (P<0.01), and on the 14th day, the EOS apoptotic rate and the expression area of FasL in the lung tissues were obviously lower either. The effects exhibited in the Western medicine group were similar to those in the Kechuanluo group. CONCLUSION: There is airway inflammation with eosinophilic infiltration in asthmatic mice, accompanied with suppressed apoptosis and delayed apoptosis. Kechuanluo can induce and accelerate EOS apoptosis in early inflammation by inhibiting eosinophilic inflammation, improving Fas expression in EOSs and FasL expression in the lung tissues, and reducing Bcl-XL expression in EOSs.

Objective To investigate the application value of warming humidification oxygen therapy in patients after tracheotomy.Methods Forty-one patients after tracheotomy were collected consecutively for this study.All patients were randomly assigned into the treatment group (21 cases)and the control group (20 cases).The treatment group adopted warming humidification oxygen therapy,and the control group adopted common airway moistening method.Moist effect judgment,viscosity of sputum,phlegm scab formation,respiratory irritation symptoms,airway mucosal bleeding,air way resistance,lung infection cases,average days in the ICU were investigated.Results There were statistically significant differences in moist effect judgment,viscosity of sputum,phlegm scab formation,respiratory irritation symptoms,airway mucosal bleeding,air way resistance,lung infection cases,average days in the ICU between the two groups.Conclusions Warming humidification oxygen therapy is more suitable to patients with tracheotomy than common airway moistening method.

Objective To explore medication rule of vertigo and headache of TCM master Yan Zhenghua.Methods In this study,44 prescriptions for vertigo and 32 prescriptions for headache were collected and analyzed based on ACCESS database.Results The three most commonly used herbs in prescription for vertigo are red peony root (100％),white peony root (79.5％),and gastrodia tuber (65.9％),and the most commonly used two-drug compatibility is red peony root and gastrodia tuber (61.4％) ; the three most commonly used herbs in prescription for headache are red peony root (68.8％),tribulus terrestris (68.8％),and white chrysanthemums (65.6％),and the most commonly used two-drug compatibility is red peony root and tribulus terrestris (59.4％).Conclusion Professor Yah Zhenghua's basic governing method to treat vertigo and headache ispromote blood circulation and relieve painandpacify the liver to subdue yang.

Many problems have been found in the inspection of assets in universities conducted　by the Ministry of Finance,Accordingly,some measures were taken to solve the problems,and as a　result the management was enhanced and the efficiency of the assets were improved.The author,　working in the front line of the inspectiong,reported the experience and in-depth reflection over the　experience to propose for the management of laboratories in medical colleges.

10.3969/j.issn.2095-4344.2013.26.020

Objective To explore the clinical application value of serum procalcitonin (PCT) detection in diagnosis of neonatal infection disease.Methods The serum PCT,CRP values,routine blood test,blood cultures of 50 children with suspected infection was detected at admission and five days after admission.According to the clinical manifestations and the test results,50 children were divided into the bacterial infection group (32 cases) of and non-bacterial infection group (18 cases),and the value of PCT,CRP,routine blood test,blood culture was compared.Results Of bacterial infection group at admission,the serum PCT values in 27 cases were more than or equal with 2μg/L,white blood cell (WBC) increased in 6 cases,CRP of 7 cases were positive,blood cultures (3d) of 4 cases were positive,and of non-bacterial infection group at admission,the serum PCT value in 2 cases were more than or equal with 2μg/L,WBC increased in 2 cases,blood cultures (3d) of 1 case was positive ; Of bacterial infection group 5 days after admission,the serum PCT value in 23 cases were more than or equal with 2μg/L,WBC increased in 15 cases,CRP of 6 cases were positive,blood culture (5d) of 9 case were positive,and of non-bacterial infection group 5 days after admission,the serum PCT value in 1 cases was more than or equal with 2μg/L,WBC increased in 3 cases,CRP of 4 cases were positive,blood culture (5d) of 1 case was positive.At admission the cases of WBC increasing,CRP positive,blood culture positive of the two groups had no statistically significant differences (x2 =0.50,0.19,0.62,all P ＞ 0.05),but the cases of the PCT value which was more than or equal with 2μg/L had statistically significant differences between the two groups (x2 =25.30,P ＜ 0.05).5 days after admission the cases of WBC increasing,PCT,blood culture positive of the two groups had no statistically significant differences (x2 =4.39,20.30,8.56,all P ＜ 0.05),but the cases of the CRP value significant differences between the two groups (x2 =0.83,P ＞ 0.05).Conclusion The detection of serum PCT can be used as an important index of diagnosis for early neonatal infections,and its sensitivity and specificity are better than,blood routine and blood culture.

Objective To investigate the expressions of signal transducer and activator of transcription factor 3 (STAT3),Bcl-2,and matrix metalloproteinase 2 (MMP2) in colorectal adenomas and adenocarcinomas,and the relationship of those factors with the colorectal clinicopathological features and prognosis of intestinal adenocarcinomas,and explore their roles in invasion,metastasis,and prognosis of a colorectal cancer.Methods The samples were selected from Dongyang City People's Hospital from January 2011 to January 2012,including 50 cases of paraffin-coded colorectal mucosas with chronic inflammation,50 cases of paraffincoded colorectal adenomas,and 100 cases of paraffin-coded colorectal adenocarcinomas (35 cases with metastasis and 65 cases without distant metastasis).Envison two method was used to detect the expressions of STAT3,Bcl-2,and MMP2 in each sample.Results The expressions of STAT3,Bcl-2,and MMP2 in colorectal adenomas and adenocarcinomas were significantly higher than those in colorectal mucosas with chronic inflammation (P ＜ 0.05).The expressions of STAT3 and MMP2 in colorectal adenocarcinomas with distant metastasis were significantly higher than that those without distant metastasis (P ＜ 0.05).The expression of BCL-2 had no significant difference between colorectal adenocarcinomas with and without distant metastasis (P ＞ 0.05).Conclusions STAT3,Bcl-2 and MMP2 were associated with occurrence and development of colorectal carcinoma.STAT3 was associated with distant metastasis of colorectal carcinoma and might indicate a poor prognosis.STAT3 might be used as a candidate clinical sign for recurrence,metastasis,and poor survival prognosis of colorectal adenocarcinoma.

Objective:To evaluate the usefulness of Periocheck in clinical application before and after periodontal curettage.Methods:Periocheck was applied in 65 sites in 65 adult patients with periodontitis within 15 min before and after scaling and root planing to monitor peptidase activity produced by Porphyromones gingivalis (Pg),Treponema denticola(Td) and Bacterides forsythus (Bf) in subgingival plaque. The peptidase activity was determined both spectrophotometrically and by using color standard.Results:The peptidase concentrations (Try U/L) before and after treatment were 0.44±0.23 and 0.34±0.26 (P<0.05),while Periocheck positiveness was found in 65/65 and 43/65 (P<0.01) of the patients,respectively.After treatment clinical parameters such as GI, PD,AL and peptidase concentration decreased (P<0.05) in 22 Periocheck positive patients,while those did not (P>0.05) in 43 negitive.Conclusion:Periocheck might provide a promising chair-side monitoring tool for the evaluation of scaling and root planing.