Epstein-Barr virus (EBV) is a DNA virus linked to a variety of human malignant tumors.In the 2008 WHO classification of lymphatic hematopoietic tissue tumor,EBV positive DLBCL of the elderly had been a new variant.The characteristic of this variant is different from other DLBCL subtypes.This paper summarized the etiology,pathogenesis,clinicopathologic features,immunophenotype,genetic changes,differential diagnosis and prognosis of EBV positive DLBCL of the elderly.

Objective:To study the characteristic of energy metabolism in moderate and severe OSA before and after CPAP. Methods:The REE was measured before and after 5-8 week treatment by CPAP in OSA patients. Health staff of single obesity in the hospital were as control. Results:During sleep, the enengy-metabolism was increased significantly in OSA patients. After treatment by CPAP, it was decreased (P

Objective To assess the characteristics of biochcmical parameters of bone metabolism in patients with inflammatory bowel disease (IBD) and the relationship between osteoporosis and the reduction of bone mass so as to identify the risk factors for osteoporosis in IBD. Methods Sixty-nine patients with IBD[49 with Crohn disease (CD) and 20 with ulcerative colitis (UC)] and 20 sex- and age-matched healthy subjects (control group) from the medical examination centre in 2007 were enrolled in the study. The disease activity was estimated according to CD active index and Truelove-Witts score. The biochemical markers of bone metabolism including ostecalcin (OC), 25-bydroxycholecalciferol[-25 (OH)Ds-] and cross-linked N-telopeptides of type I collagen(NTX) were tested using enzyme immunoassay. The body mass index (BMI) and bone mineral density (BMD) were also measured. Results The concentration of 25(OH)D3 was significantly lower in both CD[(44.45±39.38) nmol/L] and UC patients[(34.67±23.79) nmol/L] compared with controls[(98.42±25.84) nmol/L] (P <0.05), while NTX level was significantly higher in both CD [(58.41±15.15) nmol BCE/mmol Cr] and UC[(57. 67±10. 75) nmol BCE/mmol Cr] patients compared with controls[(30. 38±13.35) nmol BCE/mmol Cr] (P<0.05). The levels of OC and 25 (OH)D3 in patients treated with steroids[(17. 3±13. 43) ng/ml and (26. 99 ± 9. 12) nmol/L, respectively] were lower than those in patients untreated with steroids[(27.33 ± 16.86) ng/ml and (45.33±39.03) nmol/L,respectively] . BMD examination revealed that the incidence of osteoporosis in CD or UC groups were 14.3%(7/49) or 3/20,respectively, with no difference between two groups (P>0. 05). The T score of lumbar spine in patients treated with steroids (-1.19±0. 93) was significantly lower than that in patients untreated with steroids (-0.80±1.29) (P<0.05), but there was no difference in T score of femoral between two groups (P>0.05). Stepwise regression analysis revealed that advanced age and reduced BMI were risk factors for osteoporosis in CD patients.Conclusions The lower BMI in patients with IBS contributes to prevalence of osteoporosis, and advanced age and reduced BMI are risk factors for osteoporosis in CD patients. NTX and 25 (OH)D3are important bone metabolic markers to predict osteoporosis in patients with IBD.

Parkinson's disease is a commonly encountered central nervous retrograde affection in elder persons. According to the theories of traditional Chinese medicine, Parkinson's disease is characterized by deficiency in the Ben (root) and excess in the Biao (branch). The Ben (root) is insufficiency of liver and kidney and deficiency of qi and blood; and the Biao (branch) is wind, fire, phlegm and stasis. Good therapeutic effects have been obtained by treatment based on syndrome differentiation, treatment with specific prescriptions and acupuncture. The further study on literatures, standardized criterion of symptoms differentiation and therapeutic effect determination as well as the screening and further research on the effective prescriptions and herbs should be paid more attention, meanwhile, the integration of herbs and acupuncture will be conducive to raise the therapeutic effect.

Bone marrow stromal antigen 2 (BST-2) is a kind of host restriction factor. Since it was discovered to be responsible for the defect in virion release of HIV-1 mutants lacking the accessory gene vpu in 2008, it was thought to mainly restrict the viruses by directly tethering viral particles at the plasma membrane. Recent reports suggest that BST-2 also can inhibit the the release of HBV particles, which are budding in the intracellular vesicles, expanding the antiviral spectrum of BST-2. Futhermore, the machanism that BST-2 used to restrict HBV release in multivesicular bodies (MVBs) is similar to that used to restrict HIV at the plasma membrane. However, HBV have evolved strategies to antagonize the antiviral action of BST-2. There are two different opinions about the antagonist. One is HBV inactivated BST-2 by HBx requiring a hepatocyte-specific environment. Another thought envelope protein HBs counteract the antiviral action of BST-2. In this review, we focus on the current advances in the anti-HBV activity of BST-2.
Animals ; Antigens, CD ; genetics ; immunology ; GPI-Linked Proteins ; genetics ; immunology ; Hepatitis B ; genetics ; immunology ; virology ; Hepatitis B virus ; genetics ; physiology ; Host-Pathogen Interactions ; Humans ; Virus Release

Cell Differentiation ; Choristoma ; pathology ; Humans ; Soft Tissue Neoplasms ; pathology ; Thymoma ; pathology ; Thymus Neoplasms ; pathology

Objective To investigate the effects of emulsified isoflurane preconditioning on lung ischemia-reperfusion (Ⅰ/R) injury in rats.Methods Thirty-two male Sprague-Dawley rats,weighing 250-320 g,were randomly divided into 4 groups (n =8 each) using a random number table:sham operation group (group S),group Ⅰ/R,intralipid group (group IL),and emulsified isoflurane preconditioning group (group EI).Lung Ⅰ/R was produced by clamping the left hilum of lungs for 1 h followed by 2 h of reperfusion in anesthetized rats.In group S,intraperitoneal normal saline 10.5 ml/kg was injected,but the hilum was only exposed 24 h later.In groups Ⅰ/R,IL and EI,intraperitoneal normal saline 10.5 ml/kg,30％ intralipid 10.5 ml/kg and 8％ emulsified isoflurane 10.5 ml/kg were injected intraperitoneally,respectively,and then the model was established 24 h later.At the end of reperfusion,blood samples were collected from the left ventricle for measurement of PaO2,PaCO2,plasma malondialdehyde (MDA) concentration (by ELISA).The rats were then sacrificed,and left lungs were removed immediately for microscopic examination and for determination of myleoperoxidase (MPO) activity (using colorimetric method) in pulmonary specimens.Wet/dry lung weight ratio (W/D ratio) was calculated.Results Compared with group S,W/D ratio,MPO activity,plasma MDA concentration and PaCO2 were significantly increased,and PaO2 was decreased in the other three groups.Compared with group Ⅰ/R,W/D ratio,MPO activity,plasma MDA concentration and PaCO2 were significantly decreased,and PaO2 was increased in IL and EI groups.W/D ratio,MPO activity,plasma MDA concentration and PaCO2 were significantly lower,and PaO2 was higher in group EI than in group IL.The pathological changes of the lung were significantly attenuated in group EI compared with group Ⅰ/R.Conclusion Emulsified isoflurane preconditioning can reduce lung Ⅰ/R injury in rats,and inhibition of pulmonary inflammatory responses and of production of oxygen free radicals are involved in the mechanism.

Objective To investigate the status of Epstein Barr virus (EBV) latent infection, the subtypes of EBV, and the expression of EBV genome in intestinal T cell lymphoma (ITCL). Methods In situ hybridization for EBER1/2 and polymerase chain reaction for EBV nuclear antigen gene (EBNA 3C) of two EBV subtypes were performed in forty two cases of ITCL. Some cases which were positive in both in situ hybridization and immunohistochemistry were detected by double staining. Amplified product of EBNA 3C gene was analyzed by DNA sequencing. For immunohistochemical staining, EBNA 2 and LMP 1 monoclonal were applied. Results EBV infection was detected in 41 of the 42 cases ( 97.6% ). Thirty eight cases ( 90.5% ) exhibited specific bands by polymerase chain reaction, in which 32 (84.2%) were EBV type A, 2 (5.3%) were type B and 4 ( 10.5% ) were mixtures of type A and B. There were seldom base insertion and base deletion in EBNA 3C gene. In 36 ( 85.7% ) cases, the tumor cells showed positive in EBER1/2 ISH, and they were demonstrated to express CD 45 RO, CD 4, CD 8, CD 56 , or TIA 1 by in situ hybridization immunohistochemistry double staining. The expression frequency of LMP 1 was 38.1% (16/42). None case presented EBNA 2 positive reaction. There were two patterns of EBV latent infection in ITCL, the more common one was type Ⅰ and the other was type Ⅱ. Conclusions A high level EBV latent infection is present in ITCL in China, and EBV type A frequently occurs. The patterns of EBV latent infection in ITCL are either type Ⅰ or type Ⅱ. ITCL and nasal NK/T cell lymphoma might belong to the same spectrum.

Objective To establish HPLC fingerprint of Radix Puerariae from Ankang,Shaanxi Province.Methods Inertisil C18 column(250 mm?4.6 mm,5 ?m) was used,with methanol-acetonitrile-0.25% acetic acid solution as mobile phase in a gradient elution.Flow rate was 1.0 mL/min.The wavelength was 250 nm.Results For the relative retention time and relative area of common peaks,the RSD of ten batches of crude drug samples were below 10%.The similarities accorded with the regulation.Conclusion The analytical method can be used for the quality control of Radix Puerariae and of its sterilized powder for injection.