Acupuncture Points ; Child, Preschool ; Dyspepsia ; therapy ; Female ; Fever ; therapy ; Humans ; Male ; Massage

Objective To determine the content of two original constituents of Liuwei Dihuang Wan migrating to blood. Methods HPLC- fingerprint of the preparation and drug- containing serum fingerprint were established by RP- HPLC. Based on the fingerprints, the original constituents migrating to blood were identified and determined by comparing with the standard. Results Loganin and paeonol were identified as the original constituents migrating to blood; the loganin content was 0.93? 0.01 mg/g and the paeonol was 0.37? 0.01 mg/g. Conclusion Using the constituent content migrating to blood as the parameter, this method will provide scientific evidence for improving the quality control of Liuwei Dihuang Wan. 

Objective The aim of this study was to prepare anti-snail polyclonal antibody and make it widely useful in snail detection. Methods The DNA fragment encoding human full length 264 amino acid of snail was obtained by PCR from cDNA library of human umbilical vein epithelial cells and was cloned into pGEX-4T-1 plasmid expressing glutathione S-transferase(GST).The GST-snail fusion protein was expressed by E.coli BL21 after IPTG induction and purified from total proteins of BL21 transformed by the recombinant plasmid pGEX-4T-1/snail.The New Zealand rabbit was immunized with the purified fusion protein to prepare polyclonal antiserum.The antiserum was identified by western blotting and immunofluorescent staining. Results The prokaryotic expression plasmid pGEX-4T-1/ snail was successfully constructed,and the fusion protein GST-snail was expressed efficiently.The polyclonal antibody raised in the rabbit could react specifically with snail in human cells.Conclusion The snail antiserum was of good purity with high titer and specificity which could satisfy the requirement for studying immuno-analysis on snail protein.

Objective To identify whether abnormal clinical manifestations of dysphagia in stroke patients could predict associated imaging abnormalities. Methods Clinical evaluations and videofluoroscopy were performed on 56 consecutive cases of stroke. The clinical and image manifestations of dysphagia were observed and analyzed u-sing logistic regression analysis. Results A bolus leaking from the mouth was found (P =0.037) to predict abnormal lip closure. Raising the head when swallowing (P =0.010) and dysarthria (P =0.025) were found to predict reduced tongue movement. Exertion in swallowing (P = 0.016) could predict poor laryngeal elevation. Abnormal la-ryngeal elevation (P =0.024) and reduced or absent gag reflex (P =0.005) were found to predict insufficient epiglottis tilt down. Coughing caused by swallowing could predict incomplete vocal fold closure (P =0.011) and aspiration (P = 0.042). Conclusion Videofluoroscopic manifestations could be predicted to some extend by some clinical swallowing abnormalities, which could increase the accuracy of clinical evaluation and help in the management of dysphagia in those who could not endure videofluoroscopy.

Objective To investigate the interactions of ovarian carcinoma cells and human peritoneal mesothelial cells (HPMC) involved in matrix metalloproteinases (MMP) expressions of ovarian carcinoma cells. Methods The conditioned medium (CM) of ovarian carcinoma cell SKOV3 was tested by enzyme-linked immunosorbent assay (ELISA) for transforming growth factor ?1 (TGF-?1). The impact of SKOV3-CM in the presence or absence of TGF-?1 neutralizing antibody on fibronectin (Fn) gene expression of HPMC was studied by RT-PCR. HPMC were pretreated with serum-free medium, SKOV3-CM, SKOV3-CM+TGF-?1 neutralizing antibody, and SKOV3-CM+IgG, then the supernatant was collected as HPMC-CM1, HPMC-CM2, HPMC-CM3 and HPMC-CM4. SKOV3 were incubated with different HPMC-CM, HPMC-CM1+antibody against Fn or HPMC-CM1+IgG. MMP-2 and MMP-9 gene mRNA expressions and protein expressions of SKOV3 were detected by RT-PCR and ELISA respectively. Results TGF-?1 in SKOV3-CM was (236?22) ng/L. Fn gene mRNA expressions of HPMC before and after stimulation by SKOV3-CM were 1.328?0.025 and 2.643?0.051, and the latter was higher than the former (P

Objective To research the expression of glucose regulating protein 78(GRP78) in livers of carbon tetrachloride(CCl-4) induced hepatocirrhosis rats.Methods Healthy adult male Wistar rats were randomly divided into control group(n=5)and hepatocirrhosis group(n=5).Oily solution with 0.12ml per 100g rat weight was subcutaneous injected in control group,and 60% carbon tetrachloride oily solution with 0.3ml per 100g rat weight were subcutaneous injected in hepatocirrhosis group for 20 weeks.At the first day of 21 weeks,all rats were killed.Each blood was collected from right ventricle and some hepatic function was measured.The morphological structure in hepatic middle lobule were observed through the staining of H E and Sirius red.The expressions of GRP78 was detected by immnunohistochemistry,Western blotting and RT-PCR.Results The serum ALT in 20 weeks hepatocirrhosis group(278.2?88.42) was evidently higher than that in control group(154.8?9.94,t=3.10,P

Objective To study the changes of biological characteristics of CD34~+/CD133~+/VEGFR-3~+ lymphatic endothelial progenitor cells(EPCs) isolated from umbilical cord blood after induction with VEGF-C and investigate the mechanisms of EPCs differentiation toward lymphatic endothelial cells. Methods Mononuclear cells were isolated from umbilical cord blood using density centrifugation with Percoll solution.CD34~+/CD133~+/VEGFR-3~+ cells were sorted with FACS.Differentiation of the cells was induced with VEGF-C.The ultrastructural changes of the cells were viewed under scanning and transmission electron microscopes.Changes in expression of markers of the cells were viewed under confocal laser scanning microscope. Results Lymphatic EPCs isolated from umbilical cord blood expressed CD34,CD133 and VEGFR-3.At 7 day after induction with VEGF-C,CD34~+/CD133~+/VEGFR-3~+ cells were shuttle-like,there were a lamellipodium,numerous filopodia and many short microvilli.Caveolae were observed.Mitochondrion and rough endoplasmic reticulum were rich in cytoplasm.At 14 day after induction,the cells demonstrated appearance of the endothelial cell and expressed lymphatic endothelial specific makers LYVE-1 and 5'-nucleotidase,the expression of CD133 disappeared.Weibel-Palade body was observed in the cytoplasm of the cell.Conclusion There are CD34~+/CD133~+/VEGFR-3~+ lymphatic EPCs in human umbilical cord blood.The cells may differentiate into lymphatic endothelial cells through VEGF-C/VEGFR-3 signaling pathway after induction with VEGF-C.

Objective To observe the curative effect of Bitongding capsule for RA. Methods 100 cases were randomly divided into 2 groups. The control group (30 cases) was treated by Zhengqing Fengtongning, and the treatment group (70 cases) was treated by Bitongding capsule. One course was three months and two courses of treatment was statistics date. Results The total effective rate of 3 months was 91.43% in treatment group and 76.67% in control group. AF and IgG of treatment group were improved obviously (P 0.05), while the clear effective rate was 65.71% in treatment group and 36.67% in control group (P

0.05),but the difference of serum low density lipoprotein cholesterol(LDL-C),glucose(Glu)and uric acid(UA)levels was significant(P