Objective To explore the correlation of blood glucose variability and TCM syndromes in type 2 diabetic patients. Methods A total of 64 type 2 diabetic patients from China-Japan Friendship Hospital during Jan. 2010 to Dec. 2013 with complete clinical data were measured by continuous glucose monitoring system (CGMs) for 3 days. Patients were divided into empirical group and deficiency group. The empirical group included heat excess and fluid deficiency syndrome, phlegm-dampness stagnation syndrome and blood stasis syndrome. The deficiency group included qi-yin deficiency syndrome and yin-yang deficiency syndrome. The mean blood glucose (MBG), and mean amplitude of glycemic excursions (MAGE) were compared between the groups and types in each group, and the correlation with TCM syndrome types were analyzed. Results There was no significant difference in MBG between two groups (P>0.05). MAGE in deficiency group was higher than that in empirical group (P<0.05). No difference of MAGE existed among TCM syndrome types in empirical group (P>0.05), but in deficiency group, MAGE in yin-yang deficiency syndrome was higher than that in qi-yin deficiency syndrome (P<0.05). Conclusion Blood glucose variability has no correlation with MBG in type 2 diabetic patients. Glucose showed significant variability in deficiency group, and the most serious glucose variability was found in type of yin-yang deficiency syndrome.

0.05).Conclusion CO2-independent culture cell system is parallel to CO2-dependent culture cell system in their effects on cell proliferation and gene expression.

Objective:To investigate the pathophysiological mechanism,clinical menifestation and radiographic diagnosis of cholesterol granuloma following chronic supperative otitis media.Method:Six cases of CG following chronic suppurative otitis media, confirmed by surgery and pathology,were reviewed and analyzed.Result:CG frequently accompanied with other middle ear diseases，and was shown as a high signal intensity on both T1-and T2-weighted images in magnetic resonance imaging(MRI).Conclusion:It was postulated that the obstruction of pneumatized temporal bone air cells,caused by other middle ear diseases such as cholesteatoma and tympanosclerosis,might be the pathophsiological mechanism of CG.The evaluations of computed tomography(CT) and clinical manifestation were limited to distinguish CG from cholesteatoma or other neoplasm,while the MRI can be of great value to characteristic diagnosis.

Objective To explore the lean management model for the application of National Natural Science Foundation of China (NSFC).Methods Under the framework of lean management,Peking University Third Hospital (PUTH) tapped potential through the earlier stage survey and policy guidance,focused on improving efficiency based on discipline and talent,curriculum service were also provided to bolster application quality.This paper reviewed the NSFC application situation of PUTH in 2014 2016 and compared with 2011-2013,summarized the management experience.Results All the indexes increased significantly during 2014-2016,also achieved a breakthrough in talent projects.Compared with 2011 2013,the application number of 2014-2016 increased by 34.8％,meanwhile,both the support number and amount of NSFC increased 44.2％ and 111.6 ％ respectively.We also achieved 71.8％ growth and added 5 experts in high end talent projects.Conclusions The lean management significantly improves the management efficiency,and we hope to make reference to other clinical hospitals in promoting basic research management.

Objective To determine how genetic polymorphisms in norepinephrine transporter (NET) gene influence the response of antidepressant treatment and how they interact with childhood trauma and recent life stress in a Chinese depressive patients.Methods 281 Chinese Han depressive patients received single antidepressant drugs for 6 weeks.Hamilton Depression Scale-17 (HAMD-17),the Childhood Trauma Questionnaire short term (CTQ-SF) and the Life Events Scale (LES) were used to evaluate severity of depressive symptoms and the occurrence of stressful life events respectively.Three single nucleotide polymorphisms (SNPs) in norepinephrine transporter were genotyped.Associations of single locus and haplotypes with antidepressant treatment response were analyzed using UNPHASED 3.0.13.The interaction of gene and life stress was analyzed by SPSS13.0 software.Results One NET SNP rs2242446 was significantly associated with antidepressant response in this Chinese male sample(0.4118vs0.2375,x2=7.046,P=0.0079,OR=0.445,95％ CI (0.243-0.815)),as was the haplotype CG(rs2242446 and rs5569;x2 =5.886,P=0.0153,OR=0.457,95％ CI (0.198-1.054)) and another haplotype CG-G(rs2242446,rs1532701 and rs5569;x2=5.360,P=0.0206,OR=0.530,95％ CI (0.202-1.386)) of NET in male samples.The NET SNPs rs5569 demonstrated interaction with childhood trauma to influence antidepressant response(β=-2.727,SE =1.195,P=0.023,OR=0.065,95％ CI (0.006-0.681)).Conclusion Antidepressant drug response was influenced by not only NET genetic polymorphisms in norepinephrine transporter gene but also interaction between the NET genetic polymorphisms and early life stress.

Background and purpose:Estrogen receptor (ER)-positive breast cancer always presents a dilemma for resistance to endocrine therapy in a long time. The recent studies showed that the expression of estrogen-responsive ifnger protein (Efp) and polo-like kinase 3 (Plk3) had a close relationship with breast cancer development. This study was to explore the expression correlation between Efp and Plk3 in ER-positive breast cancer in order to understand the inlfuence of Efp and Plk3 on the drug resistance.Methods:The expression of Efp and Plk3 in 74 cases of ER-positive breast cancer was detected by SP immunohistochemistry. The clinical signiifcance was then analyzed. Real-time lfuorescent quantitative polymerase chain reaction (RTFQ-PCR) and Western blot were used to detect the expression of Efp and Plk3 in ER-positive MCF-7 cells.Results:No signiifcant relationship was found between Efp and Plk3 expression and the clinicopathological features of 74 cases of ER-positive breast cancer (P>0.05). The number of cases whose Efp showed positive expression was 51 (68.9%), while the number of cases whose Plk3 showed positive expression was 23 (31.1%). Chi-square test analysis showed the expression of Efp and Plk3 was negatively correlated in 74 cases of ER-positive breast cancer (χ2=8.837,P<0.05). The result of RTFQ-PCR showed that the expression of Efp mRNA in MCF-7 cells was up-regulated by estrogen stimulation, whereas Plk3 mRNA was not changed. The result of Western blot showed that the expression of Efp protein in MCF-7 cells was increased by estrogen and MG132 stimulation, whereas Plk3 protein was decreased.Conclusion:The expression of the Efp protein is negatively correlated with Plk3 protein in ER-positive breast cancer. High expression of Efp may be involved in the resistance to endocrine therapy.

Objective:To compare the models of guinea pig allergic rhinitis induced by different allergens. Methods: Ovalbumin (OVA), 2,4-tolylene diisocyanate (TDI) and alternariaalternata was respectively used as the allergens to establish the model of guinea pigs allergic rhinitis. The conformity of the models and human allergic rhinitis was studied through the behavioral indices, such as the times of nose itches, nasal discharge flow, histological properties and serum HA and IgE indices. Results:The times of sneezing and scratching nose, serum HA and IgE in OVA group was significantly different from those in the control group (P<0. 001 or P<0. 01). Conclusion:The models of allergic rhinitis induced by OVA are the same as allergic rhinitis in typical symptoms and pathological changes.

AIM:To study the development of autologous peripheral blood stem cells in the fibrous liver after mobilizing recombinant human granulocyte colony stimulating factor and the therapeutical effect of autologous peripheral blood stem cell transplantation.METHODS:Experiments were performed at the Central Laboratory of First Affiliated Hospital,China-Japan Clinical Pathological Center and Central Laboratory of Dalian Medical University from April 2005 to April 2006.①Rat models of hepatic fibrosis were made by injecting carbon tetrachloride subcutaneously.Thirty-two successfully established rat models of hepatic fibrosis were divided into group A,group B,group C and group D with 8 in each group,male and female(half and half).②Rat models of group A were only administered with recombinant human granulocyte colony stimulating factor subcutaneously.Rat models of group B were administered with recombinant human granulocyte colony stimulating factor subcutaneously.Peripheral blood mononuclear cells containing peripheral blood stem cells were collected by density gradient centrifugation.These peripheral blood mononuclear cells were grafted in liver after labeling with PKH26-GL.Rat models of group C did not undergo any management.Serum and hepatic tissues were collected from rat models of group A,group B and group C six weeks later.Successful rat models of liver fibrosis of group D were sacrificed instantly after the last time of carbon tetrachloride administration.Serum and hepatic tissues were collected for measurement.③The alanine aminotransferase,aspartate aminotransferase,albumin and total bilirubin levels and hepatic pathological changes of rat models of all the four groups were measured.Immunofluorescent method was employed to observe the development of autologous peripheral blood stem cells in the liver.RESULTS:A total of 32 rats were involved in the result analysis.①Total bilirubin levels were lower in group A and group B than that of group C(P 0.05).②Hepatic fibrosis was significantly improved in the group A and group B as compared with the group C(P

ObjectiveTo identify the effect of Xianlinggubao Tablet on the osteoporosis bone pain and bone density in a population of 50 cases at age of 50 and above. MethodsIn a prospective study, the changes of the clinic bone pain and bone density were observed before and after 4—8 weeks treatment. Results & ConclusionsThe bone pain in 36 of 50 was relieved, but no significant difference was observed in bone density. Therefore, Xianligubao Tablet can relieve the osteoporosis bone pain, which may change the space structure of bone trabecula.