OBJECTIVE: To prepare fumeisong cream and establish its quality control method. METHODS: Ingredients including carbamide, triamcinolone acetonide acetate, camphor, and laurocapram were mixed in an oil-in-water matrix for preparation of fumeisong cream. HPLC was adopted to determine the content of triamcinolone acetonide in the cream. RESULTS: The prepared cream was white in color, with test items all in line with specifications stipulated in 2005 version of China Pharmacopoeia. The linearity concentration range of triamcinolone acetonide was 2.06～20.6 ?g?mL-1(r=0.999 6) and the average recovery rate was 99.6% (RSD=1.0%,n=9). CONCLUSION: The cream is simple and feasible in preparation techniques, and its quality is stable and controllable.

Serious market failure, as a result of insufficient incentive, exists in the orphan drugs market. Through legislation, the United States designed and established its owns systematic and linked orphan drugs incentive mechanism, which direct sat research, definition, clinical trials and approval of orphan drugs, so as to change the supply and demand status of orphan drugs. Such incentive mechanism erables the United States possess the most ap-proved orphan drugs in the world, and effectively relieves the problem of market failure of the orphan drugs. The ex-perience of the United States provides beneficial reference for the establishment and positive development of orphan drugs incentive mechanism in China.

Chimeric antigen receptor T cells (CAR-T), one of the most promising cancer immunotherapy, has attracted much attention in the 57th American Society of Hematology (ASH) annual meeting. CAR-T therapy has obtained significant effect on leukemia and lymphoma and the latest research results are also inspiring in the 57th ASH annual meeting. It is an important task that how to combine CAR-T therapy with the traditional methods of treatment and the immune checkpoint blocking antibodies and small-molecule-targeted drugs to achieve the best effect. This paper will review the progress of CAR-T in the treatment of hematologic malignancies.

Objective To explore the effect of the different doses of oxytocin(OXT) on stress model aggressive behaviour in Wistar rats.Methods According to random number table,40 pregnant rats were divided into experimental stress groups (A, B, C) and control group(D) with 10 samples in each group.Before forced swimming test, rats in experimental groups (A, B, C)were given OXT via intraperitoneal injection with 0.3 mg/kg, 1.0 mg/kg,2.0 mg/kg;rats in group D were given equal volume of normal saline in the same way.At the 3rd day all rats were tested with maternal aggression,including mothers' following indices as total aggression times,biting times,pinning times,attack latency and duration of attack.Elevated plus maze (EPM) test was applied to analyze the behavioral changes.Results In terms of the duration of attack and attack latency,the indexes of group A and group D were significantly different from relevant data ((147.60±23.92) s and(79.70±9.88) s, P＜0.05), while other indexes had no difference(P＞0.05).The duration of attack and attack latency had a significant difference ((3.10±0.87) times, (13.60±5.14)times, (91.30±9.74)s and(167.20±30.02) s, P＜0.01) between group B and group D except total aggression times(P＞0.05).Group C and group D in given indexes had significant differences showing (6.40± 1.34) times, (15.10±4.35) times, (23.70±3.46) times, (49.80± 5.53) s and (215.60± 39.55) s (P＜0.01).Group A and group B in given indexes had significant differences (P＜ 0.05);and group B and group C had a significant difference (P＜0.01)except pinning times(P＞0.05).In EPM ,the open arm entry ratio and the open arm time ratio of both group A and group D were significantly different from respective percentage (0.47±0.13) and (0.13±0.05) (P＜0.01).The significant difference was found in group B and group D(P＜0.01);group A and group B had a statistical difference(P＜0.05);there was no difference in both groups in open arm time ratio(P＞0.05).Conclusion Oxytocin potentially mediates maternal aggression by attenuating stress, and improves the intimate bonding between mother and pups,which means dose dependency of OXT in this process.

Objective To establish the quality standard of Huangqi-Changyu'an capsule. Method Thin layer chromatography was used to identify psoralen, evodia mtaecarpa, and nutmeg in Huangqi-changyu'an capsule. HPLC was used to determine the content of psoralen. A Hypeisil BDS C18 column(200 mm×4.6 mm,5 μm) was used with mobile phase consisted of acetonitrile-water (30:70) at the rate of 1 ml/min. The detection wavelength was set at 245nm with the column at room temperature. Results The liner range of psoralen was 3.572~53.58 μg/ml (r=0.999 9) with average recoveries of 99.04%, and the RSD was 0.8%. The liner range of isopsoralen was 3.796~56.94 μg/ml (r=0.999 9) with average recoveries of 99.20%, and the RSD was 0.6%. Conclusion This method is sentivive, accurate and rapid for the quality control of Huangqi-Changyu'an capsule.

Objective To investigate the clinical efficacy of kidney-reinfocing and Du meridian-regulating acupuncture plus ginger moxibustion in treating ankylosing spondylitis. Methods Sixty patients with ankylosing spondylitis were randomly allocated to treatment and control groups, 30 cases each. The treatment group received kidney-reinfocing and Du meridian-regulating acupuncture plus ginger moxibustion and the control group, medication. After three months of treatment, the clinical therapeutic effects were compared between the two groups. Results The cure and marked efficacy rate and the total efficacy rate were 76.7%and 100.0%, respectively, in the treatment group and 53.3%and 70.0%, respectively, in the control group. There were statistically significant differences in both the rates between the two groups (P<0.05). Conclusion Kidney-reinfocing and Du meridian-regulating acupuncture plus ginger moxibustion is an effective way to treat ankylosing spondylitis.

Objective:To explore the relationship between periodontitis and coronary heart disease (CHD)by meas-uring changes of serum inflammatory cytokine levels.Methods:A total of 197 patients from our hospital were en-rolled,including CHD + periodontitis group (123 cases),pure CHD group (CHD group,39 cases)and pure peri-odontitis group (periodontitis group,35 cases).Another 45 healthy subjects were enrolled as healthy control group. Concentrations of tumor necrosis factor-α(TNF-α),interleukin-6 (IL-6),serum amyloid-a (SAA),high sensitive C reactive protein (hsCRP)and plasma fibrinogen (Fg)were measured in all groups,and levels of above inflammatory cytokines were compared among four groups.Results:Compared with healthy control group,there were significant rise in levels of above inflammatory cytokines in CHD + periodontitis group (P<0.01 all),Com-pared with CHD group or periodontitis group,there were significant rise in levels of hsCRP [(6.52±2.03)mg/L, (4.34±1.76)mg/L vs.(9.21±2.65)mg/L],IL-6 [(65.32±21.01)ng/L,(65.12±12.34)ng/L vs.(85.76± 25.96)ng/L]and TNF-α[(9.75±3.74)ng/L,(9.34±4.55)ng/L vs.(13.46±8.12)ng/L],SAA [(2.25±1.2) pg/ml,(2.22 ±1.02)pg/ml vs. (2.85±1.45)pg/ml],P<0.05 all in CHD + periodontitis group;there were no significant difference in all serum inflammatory cytokine levels between CHD group and periodontitis group (P>0.05 all).Conclusion:Serum inflammatory cytokine levels significant rise in palients with periodontitis,it is related with coronary heart disease.

Intestinal fibrosis is the complications of inflammatory bowel disease (IBD), intestinal myofibro-blast cells are the key to intestinal fibrosis .Intestinal myofibroblasts and its interaction with inflammatory cells play an im-portant role in IBD-related intestinal fibrosis .

BACKGROUND:In the condition of exercise physiology, adenosine triphosphate-sensitive potassium (KATP) channel plays an important role in many aspects, such as regulation of coronary artery tension, exercise-induced myocardial protection effect and delay of skeletal muscle fatigue. OBJECTIVE:To review and investigate the role of KATP in exercise in order to provide theoretical reference for understanding mechanism underlying exercise regulation of body’s metabolism. METHODS: A computer-based online search of PubMed and VIP databases was performed for articles related to molecular structure, biological function and regulatory role of KATP as wel as correlation of KATP with coronary artery, myocardium, skeletal muscle fatigue and exercise ability published from January 1991 to June 2014. The keywords were “KATP channels; adenosine triphosphate; sports; myocardium; ion channels” in English and Chinese, respectively. Finaly, 42 relevant articles were included in result analysis. RESULTS AND CONCLUSION: Coupling with celular energy metabolism and cel membrane excitability, KATP channel is one of the effectors for myocardial protection in response to various physiological and pathological stresses. Prolonged endurance training can increase the expression of skeletal muscle and myocardial KATP, which may be an adaptive performance of the myocardium and skeletal muscle in response to exercise stress. KATP may participate in regulation of coronary blood flow. Myocardial KATP plays an important protective role for aleviating myocardial ischemia-reperfusion injury induced by exercise. When skeletal muscle fatigue occurs, the activation of KATP is helpful to prevent muscle fiber damage and cel death due to excessive consumption of ATP, in favor for the fast recovery from fatigue. The relation between KATP and exercise ability stil needs further research.

With the further study on heterogeneity and molecular mechanism of diffuse large B-cell lymphoma (DLBCL),many novel targeted drugs have been developed,which also showed clinical benefit in early-stage studies.Therapeutic targets for DLBCL mainly include the surface antigens of B lymphocyte,B cell receptor signaling and the cellular microenvironment.Several new clinical trials about targeted therapy for DLBCL had been reported in the 55th ASH annual meeting,and the results were encouraging.The advances in this field will be summarized in this paper based on the new reports in the 55th ASH annual meeting.