The leaders of our sanitarium pay much attention to the work of military medical metrology which has formed into a normative,scientific,institutional managing system and the work of military medical metrology develops according to the laws and rules. In continuous five years,the probability of metrology equipments being checked reached into 100%. The development of military medical metrology work is great and healthy.

0.05).Motor block with ropivacaine was recorded earlier than that with bupivacaine(P0.05).Conclusion Ropivacaine is superior to bupivacaine in postoperative analgesia and in motor recovery in children undergoing subabdominal surgery.

Objective To observe clinical effect of acupuncture combined with rehabilitation training on spastic cerebral palsy. Methods 160 patients with cerebral palsy were divided into acupuncture group(n=80) and control group(n=80). The acupuncture group received acupuncture, including the acupoints such as Baihui, Sishengcong, Yamen, Fengchi, etc., and combined with function training. The control group received Bobath appoach as rehabilitation training. Two groups were treated for 3 months. Results The acupuncture group(n=77) showed powerfully for 26 cases, effective for 46 cases, and invalid for 5 cases, and the efficient rate was 93.5%. The control group (n=78) showed powerfully for 20 cases, effective for 45 cases, invalid for 13 cases, and the efficient rate was 83.3% (P<0.05). Conclusion Acupuncture combined with rehabilitation training can facillitate to reduce muscle tone of spastic cerebral palsy, and improve effect of rehabilitation.

Humans ; Otitis Media ; etiology ; pathology ; Toll-Like Receptors

Child, Preschool ; Female ; Fructose Intolerance ; pathology ; Humans

Objective To establish an in vitro model of primnary osteoblasts fluorosis and to detect the influences of different doses of fluorosis on promoter methylation,transcription and expression of p16,then study the epigenetic effects of p16 gene on skeletal fluorosis.Methods Osteoblasts were isolated from Sprague-Dawley neonatal rats by enzyme digestion,and identified by morphology,alkaline phosphatasc staining and alizarin red staining.Osteoblast were treated with 0 (control group),200,400,800 and 1 600 μmol/L NaF for 72 h.The pl6 gene promoter region was amplified in the transcription initiation site-88-+ 143 region by bisulfatesequencing polymerase chain reaction (BSP).The mRNA transcription and the protein expression of p16 were detected by real-time quantitative PCR and Western blotting.Results Among the groups of osteohlasts treated with 200,400,800,1 600 μmol/L NaF,the positive rates of DNA methylation of promoter region in p16 gene of osteoblasts were 5.88％ (10/170),12.94％ (22/170),17.65％ (30/170) and 33.53％ (57/170),respectively.No DNA methylation was observed in the control group.There were significant differences between the control group and the NaF-treated osteoblasts groups (x2 =92.87,P ＜ 0.05).Average levels of p16 mRNA were 1.050 ± 0.073,0.869 ±0.037,1.065 ± 0.118 and 0.786 ± 0.148 in 200,400,800 and 1 600 μmol/L NaF-treated osteoblasts groups,compared with the control group (1.110 ± 0.315),there were significant differences among groups (all P ＜ 0.05) and 1 600 μmol/L NaF-treated osteoblasts group was much lower than other groups (P ＜ 0.05).Average levels of p16protein were 1.190 ± 0.050,1.214 ± 0.058,1.122 ± 0.123 and 0.320 ± 0.074 in 200,400,800 and 1 600 μmol/LNaF-treated osteoblasts groups,compared with the control group (1.115 ± 0.057),there were significant differences among groups (all P ＜ 0.05) and 1 600 μmol/L group was much lower than other groups.Conclusion NaF can cause hypermethylation in the promoter region of p16 gene,then suppress the expression of mRNA and protein,which might be one of the important mechanisms of cell proliferation change and cell cycle disorder in skeletal fluorosis.

Objective To understand the microorganism pollution in the indoor air in the campus of Liaocheng University. Methods In April and May, 2004, the content of airborne microbes in the indoor air in Liaocheng University campus was determined by the gravity plate method. Results The average content of bacteria in the indoor air was 2.39?103 cfu/m3, the average content of mildew was 0.62?103 cfu/m3. The predominant microorganism in the internet bars in the campus was bacteria. Conclusion In general, the indoor air quality in Liaocheng University campus is at comparative clear level.

0.05). Compared with the control group,an obvious retarded development was observed from PND21-70 and the viscera coefficient of testis were increased significantly in 2.5 or 5.0 mg/(kg?d) group (P

0.05).Compared with the control group,body weights gains from PND21-70 were decreased significantly in the 5 and 2.5 mg/kg group(P0.05).No abnormal structure of testis was observed in all exposure groups.Conclusion Exposure to TBTC during critical period for sex differentiation may decrease body gain and increase spermatogenesis and the activities of LDH and SDH.

Objective To explore the effects of gestation and lactation exposure to low dose tributyltin(TBT) on development of offspring of Kunming mice.Methods The healthy adult pregnant Kunming mice were randomly divided into 4 groups,6 in each group,they were given doses of TBT(100,10 and 1 ?g/kg) by gavage from days 6 of gestation(GD6)to the end of lactation.The number of pups and the number of male,female of each dams were determined,and the body weight of pups on PNDs 1,3,5,7,14,21 was weighed.The eye opening from PND12 and the testes descent from PND13 and the vagina opening from PND20 were examined.Results Compared with the control group,the gestational body weight gain of dams decreased in 100 ?g/kg group(P