Objective To boost the transformation and sustainable development of medical scientific achievements. Methods Investigations in the form of questionnaires were made and descriptive statistics was conducted. Results Eighty to ninety percent of the achievements awards were allocated to the research project teams or the principal prize winners. Most of the achievements transformation awards were retained by the units while only ten to twenty percent went to the principal awardees and only two to three percent went to the management and intermediary personnel. There also existed such problems as weak awareness of achievements transformation and lack of scientificalness in the achievements review and evaluation system, assessment system and assessment indexes. Conclusion The awareness of scientific achievements transformation should be enhanced and the transformation should be implemented on a market oriented and commercial basis; the scientificalness of achievements review and evaluation should be improved; more awards should be given and more social fundraising activities should be conducted; and scientific research management should be included among the factors to be considered when allocating money awards.

Objective:To locate the region of antigenic epitopes recognized by monoclonal antibody(Z8) against human tumor necrosis factor ?(hTNF ?).Methods:Several recombinant fusion expression vectors were constructed which contained hTNF ? mutant genes deleted different parts respectively. After induction with IPTG, the fusion proteins were expressed and analyzed by SDS PAGE and Western blot.Results:Z8 specially recognized the fusion products which contained amino acids from 92 to 157 of hTNF ?, but not GST and its fusion protein with amino acids from 1 to 91 of hTNF ?.Conclusion:The antigenic epitopes recognized by Z8 were located in the sequence region 92 157 of hTNF ?.

Osteosarcoma and ewing sarcoma are the two most common types of primary malignant bone tumors. Receptor tyrosine kinases(RTKs),including varieties of cell growth factors and proto-oncogene pro-ducts,are the high-affinity cell surface receptors which play an important role in the normal physiological func-tion of cells. A large number of studies have demonstrated that the abnormal RTKs signaling pathways may pro-mote the tumorigenesis and development of osteosarcoma and ewing sarcoma by affecting tumor cell survival, proliferation,invasion and metastasis. Targeting treatment of RTKs is a promising therapeutic approach for osteosarcoma and ewing sarcoma.

Objective To investigate the perinatal outcomes of isolated single umbilical artery (ISUA) in full-term birth. Methods We performed a retrospective study of 82 252 pregnant women (aged<35 years;singleton; full-term live birth; no known chromosomal/structural anomalies) who delivered between January 1, 2011 and January 1, 2012 in 39 hospitals in 14 cities in China. There were 273 cases with ISUA as study group and 1 092 cases with a three-vessel cord selected in a 1∶4 ratio as control group. Perinatal outcomes including delivery modes (vaginal delivery or emergency cesarean section for fetal reasons), birth weight and height, placental weight, umbilical cord length, Apgar scores at 1 min, and rate of neonatal asphyxia were analyzed retrospectively. Data were analyzed using the Chi-square test, two independent samples t-test or rank sum test. Results The incidence of ISUA was 0.3%(273/82 252). There was no difference between the two groups with regard to maternal age, parity, gravidity, smoking status, gestational age at birth, or rate of complications during pregnancy (gestational diabetes mellitus or pregnancy-induced hypertension) (all P>0.05). The birth weight in ISUA group and control group was (3 259±463) and (3 344±411) g (t=3.689), birth hight was (48.6±2.7) and (49.7±1.9) cm (t=8.368), placental weight was (508±72) and (543±153) g (t=3.114), umbilical cord length was (52.6±8.1) and (53.7±7.5) cm (t=1.983), and Apgar scores at 1 min were 9.0 (8.0-10.0) and 10.0 (9.0-10.0) (Z=11.831), respectively; the values in the ISUA group were lower than those in control group (all P < 0.05). No significant differences were observed in the rate of vaginal delivery and emergeny cesarean section for fetal reasons between the ISUA group and control group [52.8%(144/273) and 49.9%(545/1 092),χ2=0.704;19.4%(25/273) and 15.7% (86/1 092), χ2=1.162; both P>0.05]. Conclusions ISUA does not increase emergency cesarean delivery rates for the fetal reasons, but the fetal growth and development may fall behind those with a three-vessel umbilical cord. Monitoring during pregnancy and delivery should be intensified for fetuses with ISUA.

BACKGROUND: 1200009K10 gene is from the lung cDNA pool of adult mouse and announced in January 2002, but its function is still not known.The predicted protein has five domains: Kelch motif, Kelch domain, BTB/POZ domain, BTB domain and β propeller domain, all of which are involved in protein-protein interactions and some enzymatic activities.OBJECTIVE: To explore the correlation between the expression of 1200009K10 gene and retinitis pigmentosa in mice.DESIGN: Observational and comparative trial.SETTING: Institute of Zhongshan Ophthalmic Center of Sun Yat-sen University.MATERIALS: The rd mice, rds mice and passage mice from C3B mice as normal control were purchased from Jackson Laboratory (Bar Harbor,Maine 04609, USA), and raised in second class unit of Experimental Animal Center of Sun Yat-sen University.METHODS: The retinal RNA of mice was extracted and isolated and 1200009K10 gene was amplified by rapid amplification of eDNA ends method (RACE). The gene expressions in the retina of rds, rd and C3B mice were analyzed, respectively by gene-specific real-time quantitative PCR.MAIN OUTCOME MEASURES: The expression contents and patterns of 1200009K10 gene in retina of three kinds of mice.RESULTS: One clone was obtained after RACE of retinal RNA extracted from retina of mice, which had an almost identical sequence with the 1200009K10 gene. Expression of 1200009K10 gene was classified into 6stages: 7, 12, 25, 37, 50 (near sex maturity) and 150 days after born. The expressions of 1200009K10 gene in the retina of rds, rd and C3B mice were low at postnatal day 7 (P7), higher between P7-12, stable between P12-50, and finally increased a little. The expression trend and level of 1200009K10 gene of C3B mice was nearly the same with that of the rds and rd mice, and higher than that of rds and rd mice at P12. Although there were differences in expression levels of 1200009K10 gene among the three kinds of mice, the expression patterns were almost the same.CONCLUSION: 1200009K10 gene may not participate in the development of retinal degeneration.

PurposeTo determine the correlation between expression of MHC classⅠon ovarian tumor cells and T-cell infiltration in vivo on ovarian tumor micro-environment.MethodsThirty three samples of primary epithelial ovarian carcers were analysed with flow cytometry for MHC class Ⅰ expression. Immunohistochemical analysis was performed using monoclonal antibodies that recognize leukocyte differentiation antigens ( LCA+ , CD3 + ) on cryostat sections of primary epithelial ovarian carcinoma. The results were compared with the expression of MHC class Ⅰ expression. ResultsClose correlation were revealed bewteen the expression of MHC class Ⅰ molecules and the numbers of infiltrating LCA+ positive cells ( r = 0. 846, P = 0. 000 1) and CD3 + cells ( r = 0. 738, P = 0. 000 1 ).ConclusionsExpression of MHC class Ⅰ molecules may influence the status of human immunosurveillance in tumor micro-environment on ovarian carcinoma and the assay may be helpful in selection patients for adoptive immnotherapy.

Proximal transparent cap of endoscope has an important effect in endoscopic diagnosis and treatment, including endoscopic submucosal tumor ligation, endoscopic mucosal resection, endoscopic removal of foreign body, endoscopic variceal ligation and sclerotherapy, endoscopic submucosal dissection, peroral endoscopic myotomy, endoscopic submucosal dissection through tunnel.This article reviewed the effect of different transparent caps in endoscopic diagnosis and treatment.

Objective To investigate the effect of combined medication of psychological intervention and retention enema on wound healing and plasma endotoxin in patients with thrombotic hemorrhoid. Methods According to the different postoperative intervention will be January 2014-2016 year in December with hemorrhoids thrombosis in our hospital group in 90 cases as control group with normal saline retention enema,the observation group with Chinese medicine retention enema+psychological intervention;the comparative analysis of two groups of patients with various experimental data and detailed records,discusses the thrombosis of hemorrhoids after the drug retention enema combined with psychological intervention effects on wound healing and plasma endotoxin. Results The observation group(traditional Chinese medicine retention enema plus psychological intervention)clinical treatment effect is better than that of control group(saline enema)clinical curative effect,clinical symptoms of the patients were better than those in control group,plasma endotoxin level was lower than the control group,the difference between groups was statistically significant (P<0.05). Conclusion Thrombosis after hemorrhoid surgery patients choose herbal retention enema+psychological intervention clinical effect significantly ,can effectively promote wound healing ,and fully reduce plasma endotoxin.

To reveal the mechanism of Cu enrichment of alfalfa, in-situ micro-X-ray fluorescence spectrometry (μ-XRF) and fractional extraction were used to explore in-situ Cu distribution information in alfalfa seedlings and different combination forms of Cu in organs.The results showed that alfalfa roots were enriched with Cu up to 12.06 mg/g, which was 8 times of stem and 4.9 times of leaves.The in-situ μ-XRF result showed that the root of alfalfa was the main site of enrichment of Cu, and there was a barrier of Cu at the rhizome junction to alleviate the toxic effect of excess Cu on the shoots.Excess Cu (more than 50 μmol/L) also inhibited the uptake of Zn and Ca in alfalfa, enhanced the uptake of Fe in alfalfa, but had no obvious effect on the uptake of K and Mn.Plant fractional extraction showed that the Cu in root cells was mainly fixed in the form of insoluble residues (41%) and cell wall chelate (20%), while in the stem, Cu existed as four forms including hydrophobic protein binding, cell wall binding state, residual state and water-soluble state, which further reduce the excess transport of Cu to the leaves.In the leaves, excess Cu in the leaf cells was mainly in the vacuole and insoluble residue to achieve tolerance and detoxification to Cu.

Objective To explore the related factors that affect the incidence of uterine fibroids to provide a theoretical basis for the clinical diagnosis and prevention.Methods Based on the clinical data selected from June 2014 to July 2016 in pathology of our hospital,159 cases of patients were confirmed with uterine fibroids,a comparative study was employed and the single factor test and the logistic regression analysis were both used to analyze the related risk factors that affecting the incidence of uterine fibroids.Results There were all significant level (P ＜ 0.05) between case and control groups in 40 years to 50 years,the number of abortions (≥2) as well as gynecological diseases,which were the independent factors for the incidence of uterine fibroids.In the present,the regression coefficients of former two factors were 0.186 (P ＜ O.05),and 0.584 (P ＜ 0.05),respectively.The corresponding regression coefficients of breast hyperplasia,vaginitis,cervicitis,endometritis and pelvic inflammatory disease,and other gynecological diseases and gynecological diseases were 0.221 (P ＜ 0.05),O.363 (P ＜ 0.05),O.539 (P ＜ 0.05),0.361 (P ＜ 0.05),and 0338 (P ＜ 0.05),respectively.It suggests that these were independent factors affecting the incidence of uterine fibroids.Conclusions The more the increasing age and the number of abortions,kinds of gynecological diseases infected will increase the risk of patients suffering from uterine fibroids.